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Protein

CCR4-NOT transcription complex subunit 6-like

Gene

Cnot6l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Poly(A) nuclease with 3'-5' RNase activity. Catalytic component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Involved in mRNA decay mediated by the major-protein-coding determinant of instability (mCRD) of the FOS gene in the cytoplasm. Involved in deadenylation-dependent degradation of CDKN1B mRNA. Its mRNA deadenylase activity can be inhibited by TOB1. Mediates cell proliferation and cell survival and prevents cellular senescence (By similarity).By similarity

Catalytic activityi

Exonucleolytic cleavage of poly(A) to 5'-AMP.

Cofactori

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei240By similarity1
Metal bindingi240Magnesium 1By similarity1
Metal bindingi410Magnesium 2By similarity1
Metal bindingi412Magnesium 2By similarity1
Active sitei489By similarity1
Active sitei529By similarity1
Metal bindingi529Magnesium 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • poly(A)-specific ribonuclease activity Source: MGI

GO - Biological processi

  • gene silencing by RNA Source: UniProtKB-KW
  • mRNA destabilization Source: MGI
  • mRNA processing Source: UniProtKB-KW
  • nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of cytoplasmic mRNA processing body assembly Source: UniProtKB
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

mRNA processing, RNA-mediated gene silencing, Transcription, Transcription regulation, Translation regulation

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-429947. Deadenylation of mRNA.
R-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Names & Taxonomyi

Protein namesi
Recommended name:
CCR4-NOT transcription complex subunit 6-like (EC:3.1.13.4)
Gene namesi
Name:Cnot6l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2443154. Cnot6l.

Subcellular locationi

GO - Cellular componenti

  • CCR4-NOT complex Source: UniProtKB
  • cytoplasm Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003145881 – 555CCR4-NOT transcription complex subunit 6-likeAdd BLAST555

Proteomic databases

PaxDbiQ8VEG6.
PeptideAtlasiQ8VEG6.
PRIDEiQ8VEG6.

PTM databases

PhosphoSitePlusiQ8VEG6.

Expressioni

Developmental stagei

Expressed in embryonic stem (ES) cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000034724.
CleanExiMM_CNOT6L.
ExpressionAtlasiQ8VEG6. baseline and differential.
GenevisibleiQ8VEG6. MM.

Interactioni

Subunit structurei

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits; the complex contains two deadenylase subunits, CNOT6 or CNOT6L, and CNOT7 or CNOT8 (By similarity). Interacts with CNOT1, CNOT3, CNOT7, CNOT8 and CNOT9 (By similarity). Interacts with TOB1 (By similarity). Interacts with NANOS2 (PubMed:20133598). Interacts with ZFP36 (PubMed:21078877).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Cnot7Q608092EBI-2104661,EBI-2104739

Protein-protein interaction databases

DIPiDIP-46842N.
IntActiQ8VEG6. 5 interactors.
MINTiMINT-4609627.
STRINGi10090.ENSMUSP00000108629.

Structurei

3D structure databases

ProteinModelPortaliQ8VEG6.
SMRiQ8VEG6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati57 – 78LRR 1Add BLAST22
Repeati80 – 101LRR 2Add BLAST22
Repeati103 – 125LRR 3Add BLAST23
Repeati126 – 148LRR 4Add BLAST23

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 152Required for interaction with CNOT1, CNOT3 and CNOT7By similarityAdd BLAST152
Regioni158 – 555Nuclease domainBy similarityAdd BLAST398

Sequence similaritiesi

Belongs to the CCR4/nocturin family.Curated
Contains 4 LRR (leucine-rich) repeats.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG4886. LUCA.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000294222.
HOVERGENiHBG052641.
InParanoidiQ8VEG6.
KOiK12603.
OMAiKDRGYDG.
OrthoDBiEOG091G05MA.
PhylomeDBiQ8VEG6.
TreeFamiTF323175.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56219. SSF56219. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VEG6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRLIGMPKEK YDPPDPRRIY TIMSAEEVAN GKKSHWAELE ISGRVRSLST
60 70 80 90 100
SLWSLTHLTA LHLNDNNLAR IPPDIAKLHN LVYLDLSSNK LRSLPAELGN
110 120 130 140 150
MVSLRELLLN DNYLRVLPYE LGRLFQLQTL GLTGNPLSQD IMSLYQDPDG
160 170 180 190 200
TRKLLNFMLD NLAVHPEQLP PRPWITLKER DQILPSASFT VMCYNVLCDK
210 220 230 240 250
YATRQLYGYC PSWALNWEYR KKGIMEEIVN WDADIISLQE VETEQYFTLF
260 270 280 290 300
LPALKDRGYD GFFSPKSRAK IMSEQERKHV DGCAIFFKTE KFTLVQKHTV
310 320 330 340 350
EFNQVAMANS DGSEAMLNRV MTKDNIGVAV VLEVHKELFG TGMKPIHAAD
360 370 380 390 400
KQLLIVANAH MHWDPEYSDV KLIQTMMFVS EVKNILEKAS SRPGSPTADP
410 420 430 440 450
NSIPLVLCAD LNSLPDSGVV EYLSNGGVAD NHKDFKELRY NECLMNFSCS
460 470 480 490 500
GKNGSSEGRI THGFQLKSAY ENNLMPYTNY TFDFKGVIDY IFYSKTHMNV
510 520 530 540 550
LGVLGPLDPQ WLVENNITGC PHPHIPSDHF SLLTQLELHP PLLPLVNGVH

LPNRR
Length:555
Mass (Da):63,023
Last modified:January 15, 2008 - v2
Checksum:iFDC180DB315919DB
GO
Isoform 2 (identifier: Q8VEG6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRLIGM → M

Show »
Length:550
Mass (Da):62,453
Checksum:i523CFA11207E304D
GO
Isoform 3 (identifier: Q8VEG6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: MRLIGM → M
     440-440: Y → V
     441-555: Missing.

Show »
Length:435
Mass (Da):49,397
Checksum:i2FC620EB985D4E14
GO

Sequence cautioni

The sequence BAC26790 differs from that shown. Reason: Frameshift at positions 38 and 64.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti238L → F in BAE30646 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0303241 – 6MRLIGM → M in isoform 2 and isoform 3. 2 Publications6
Alternative sequenceiVSP_030325440Y → V in isoform 3. 1 Publication1
Alternative sequenceiVSP_030326441 – 555Missing in isoform 3. 1 PublicationAdd BLAST115

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030112 mRNA. Translation: BAC26790.1. Frameshift.
AK151728 mRNA. Translation: BAE30646.1.
AC129600 Genomic DNA. No translation available.
AC149285 Genomic DNA. No translation available.
BC018506 mRNA. Translation: AAH18506.1.
CCDSiCCDS19448.1. [Q8VEG6-2]
CCDS51566.1. [Q8VEG6-1]
RefSeqiNP_001272440.1. NM_001285511.1. [Q8VEG6-2]
NP_001272443.1. NM_001285514.1.
NP_659159.1. NM_144910.2. [Q8VEG6-2]
NP_849185.2. NM_178854.4. [Q8VEG6-1]
XP_006534933.1. XM_006534870.3. [Q8VEG6-2]
XP_006534934.1. XM_006534871.1. [Q8VEG6-2]
UniGeneiMm.28374.
Mm.384746.

Genome annotation databases

EnsembliENSMUST00000113005; ENSMUSP00000108629; ENSMUSG00000034724. [Q8VEG6-1]
ENSMUST00000122003; ENSMUSP00000113821; ENSMUSG00000034724. [Q8VEG6-3]
ENSMUST00000155901; ENSMUSP00000119415; ENSMUSG00000034724. [Q8VEG6-2]
GeneIDi231464.
KEGGimmu:231464.
UCSCiuc008yfd.2. mouse. [Q8VEG6-3]
uc008yfe.2. mouse. [Q8VEG6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK030112 mRNA. Translation: BAC26790.1. Frameshift.
AK151728 mRNA. Translation: BAE30646.1.
AC129600 Genomic DNA. No translation available.
AC149285 Genomic DNA. No translation available.
BC018506 mRNA. Translation: AAH18506.1.
CCDSiCCDS19448.1. [Q8VEG6-2]
CCDS51566.1. [Q8VEG6-1]
RefSeqiNP_001272440.1. NM_001285511.1. [Q8VEG6-2]
NP_001272443.1. NM_001285514.1.
NP_659159.1. NM_144910.2. [Q8VEG6-2]
NP_849185.2. NM_178854.4. [Q8VEG6-1]
XP_006534933.1. XM_006534870.3. [Q8VEG6-2]
XP_006534934.1. XM_006534871.1. [Q8VEG6-2]
UniGeneiMm.28374.
Mm.384746.

3D structure databases

ProteinModelPortaliQ8VEG6.
SMRiQ8VEG6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-46842N.
IntActiQ8VEG6. 5 interactors.
MINTiMINT-4609627.
STRINGi10090.ENSMUSP00000108629.

PTM databases

PhosphoSitePlusiQ8VEG6.

Proteomic databases

PaxDbiQ8VEG6.
PeptideAtlasiQ8VEG6.
PRIDEiQ8VEG6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000113005; ENSMUSP00000108629; ENSMUSG00000034724. [Q8VEG6-1]
ENSMUST00000122003; ENSMUSP00000113821; ENSMUSG00000034724. [Q8VEG6-3]
ENSMUST00000155901; ENSMUSP00000119415; ENSMUSG00000034724. [Q8VEG6-2]
GeneIDi231464.
KEGGimmu:231464.
UCSCiuc008yfd.2. mouse. [Q8VEG6-3]
uc008yfe.2. mouse. [Q8VEG6-1]

Organism-specific databases

CTDi246175.
MGIiMGI:2443154. Cnot6l.

Phylogenomic databases

eggNOGiKOG0620. Eukaryota.
COG4886. LUCA.
COG5239. LUCA.
GeneTreeiENSGT00550000074364.
HOGENOMiHOG000294222.
HOVERGENiHBG052641.
InParanoidiQ8VEG6.
KOiK12603.
OMAiKDRGYDG.
OrthoDBiEOG091G05MA.
PhylomeDBiQ8VEG6.
TreeFamiTF323175.

Enzyme and pathway databases

ReactomeiR-MMU-429947. Deadenylation of mRNA.
R-MMU-6804115. TP53 regulates transcription of additional cell cycle genes whose exact role in the p53 pathway remain uncertain.

Miscellaneous databases

ChiTaRSiCnot6l. mouse.
PROiQ8VEG6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034724.
CleanExiMM_CNOT6L.
ExpressionAtlasiQ8VEG6. baseline and differential.
GenevisibleiQ8VEG6. MM.

Family and domain databases

Gene3Di3.60.10.10. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR005135. Endo/exonuclease/phosphatase.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
[Graphical view]
PfamiPF03372. Exo_endo_phos. 1 hit.
PF13855. LRR_8. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 3 hits.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56219. SSF56219. 1 hit.
PROSITEiPS51450. LRR. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCNO6L_MOUSE
AccessioniPrimary (citable) accession number: Q8VEG6
Secondary accession number(s): Q3U9M0, Q8C0P4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.