UniProtKB - Q8VEE4 (RFA1_MOUSE)
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Protein
Replication protein A 70 kDa DNA-binding subunit
Gene
Rpa1
Organism
Mus musculus (Mouse)
Status
Functioni
As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates, that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair. Plays also a role in base excision repair (BER) probably through interaction with UNG. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance.By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 206 – 290 | OBAdd BLAST | 85 |
GO - Molecular functioni
- chromatin binding Source: MGI
- damaged DNA binding Source: UniProtKB
- G-rich strand telomeric DNA binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- single-stranded DNA binding Source: UniProtKB
GO - Biological processi
- base-excision repair Source: UniProtKB
- chromosome organization Source: MGI
- DNA repair Source: MGI
- DNA replication Source: UniProtKB
- double-strand break repair via homologous recombination Source: MGI
- hemopoiesis Source: MGI
- homeostasis of number of cells within a tissue Source: MGI
- in utero embryonic development Source: MGI
- meiotic cell cycle Source: MGI
- mismatch repair Source: UniProtKB
- nucleotide-excision repair Source: UniProtKB
- positive regulation of cell proliferation Source: MGI
- protein localization to chromosome Source: UniProtKB
- telomere maintenance Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA damage, DNA recombination, DNA repair, DNA replication |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-110312. Translesion synthesis by REV1. R-MMU-110314. Recognition of DNA damage by PCNA-containing replication complex. R-MMU-110320. Translesion Synthesis by POLH. R-MMU-174437. Removal of the Flap Intermediate from the C-strand. R-MMU-176187. Activation of ATR in response to replication stress. R-MMU-3108214. SUMOylation of DNA damage response and repair proteins. R-MMU-3371453. Regulation of HSF1-mediated heat shock response. R-MMU-5358565. Mismatch repair (MMR) directed by MSH2:MSH6 (MutSalpha). R-MMU-5358606. Mismatch repair (MMR) directed by MSH2:MSH3 (MutSbeta). R-MMU-5651801. PCNA-Dependent Long Patch Base Excision Repair. R-MMU-5655862. Translesion synthesis by POLK. R-MMU-5656121. Translesion synthesis by POLI. R-MMU-5656169. Termination of translesion DNA synthesis. R-MMU-5685938. HDR through Single Strand Annealing (SSA). R-MMU-5685942. HDR through Homologous Recombination (HRR). R-MMU-5693607. Processing of DNA double-strand break ends. R-MMU-5693616. Presynaptic phase of homologous DNA pairing and strand exchange. R-MMU-5696395. Formation of Incision Complex in GG-NER. R-MMU-5696397. Gap-filling DNA repair synthesis and ligation in GG-NER. R-MMU-5696400. Dual Incision in GG-NER. R-MMU-6782135. Dual incision in TC-NER. R-MMU-6782210. Gap-filling DNA repair synthesis and ligation in TC-NER. R-MMU-6783310. Fanconi Anemia Pathway. R-MMU-6804756. Regulation of TP53 Activity through Phosphorylation. R-MMU-68962. Activation of the pre-replicative complex. R-MMU-69166. Removal of the Flap Intermediate. R-MMU-69473. G2/M DNA damage checkpoint. R-MMU-912497. Meiotic Recombination. |
Names & Taxonomyi
Protein namesi | Recommended name: Replication protein A 70 kDa DNA-binding subunitShort name: RP-A p70 Alternative name(s): Replication factor A protein 1 Short name: RF-A protein 1 |
Gene namesi | Name:Rpa1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915525. Rpa1. |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000097261 | 1 – 623 | Replication protein A 70 kDa DNA-binding subunitAdd BLAST | 623 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Cross-linki | 22 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 88 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 172 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 172 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 176 | N6-acetyllysine; alternateCombined sources | 1 | |
Cross-linki | 176 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Modified residuei | 189 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 192 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 200 | PhosphothreonineBy similarity | 1 | |
Cross-linki | 229 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 253 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 268 | N6-acetyllysine; alternateBy similarity | 1 | |
Cross-linki | 268 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity | ||
Cross-linki | 276 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 340 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 393 | PhosphoserineBy similarity | 1 | |
Cross-linki | 419 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 458 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity | ||
Cross-linki | 467 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 562 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 586 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity |
Post-translational modificationi
DNA damage-induced 'Lys-63'-linked polyubiquitination by PRPF19 mediates ATRIP recruitment to the RPA complex at sites of DNA damage and activation of ATR. Ubiquitinated by RFWD3 at stalled replication forks in response to DNA damage: ubiquitination by RFWD3 does not lead to degradation by the proteasome and promotes removal of the RPA complex from stalled replication forks, promoting homologous recombination.By similarity
Sumoylated on lysine residues Lys-458 and Lys-586, with Lys-458 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombination. Desumoylated by SENP6 (By similarity).By similarity
Keywords - PTMi
Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q8VEE4. |
MaxQBi | Q8VEE4. |
PaxDbi | Q8VEE4. |
PeptideAtlasi | Q8VEE4. |
PRIDEi | Q8VEE4. |
PTM databases
iPTMneti | Q8VEE4. |
PhosphoSitePlusi | Q8VEE4. |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000000751. |
CleanExi | MM_RPA1. |
ExpressionAtlasi | Q8VEE4. baseline and differential. |
Genevisiblei | Q8VEE4. MM. |
Interactioni
Subunit structurei
Component of the canonical replication protein A complex (RPA), a heterotrimer composed of RPA1, RPA2 and RPA3. The DNA-binding activity may reside exclusively on the RPA1 subunit. Interacts with PRPF19; the PRP19-CDC5L complex is recruited to the sites of DNA repair where it ubiquitinates the replication protein A complex (RPA). Interacts with RIPK1. Interacts with the polymerase alpha subunit POLA1/p180; this interaction stabilizes the replicative complex and reduces the misincorporation rate of DNA polymerase alpha by acting as a fidelity clamp. Interacts with RAD51 and SENP6 to regulate DNA repair. Interacts with HELB; this interaction promotes HELB recruitment to chromatin following DNA damage. Interacts with PRIMPOL. Interacts with XPA; the interaction is direct and associates XPA with the RPA complex. Interacts with ETAA1; the interaction is direct and promotes ETAA1 recruitment at stalled replication forks.By similarity
Protein-protein interaction databases
BioGridi | 212778. 7 interactors. |
CORUMi | Q8VEE4. |
IntActi | Q8VEE4. 3 interactors. |
STRINGi | 10090.ENSMUSP00000000767. |
Structurei
3D structure databases
ProteinModelPortali | Q8VEE4. |
SMRi | Q8VEE4. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the replication factor A protein 1 family.Curated
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0851. Eukaryota. COG1599. LUCA. |
GeneTreei | ENSGT00390000012403. |
HOGENOMi | HOG000162322. |
HOVERGENi | HBG010502. |
InParanoidi | Q8VEE4. |
KOi | K07466. |
PhylomeDBi | Q8VEE4. |
Family and domain databases
CDDi | cd04476. RPA1_DBD_C. 1 hit. cd04477. RPA1N. 1 hit. |
InterProi | View protein in InterPro IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA. IPR013955. Rep_factor-A_C. IPR007199. Rep_factor-A_N. IPR031657. REPA_OB_2. IPR004591. Rfa1. |
Pfami | View protein in Pfam PF04057. Rep-A_N. 1 hit. PF08646. Rep_fac-A_C. 1 hit. PF16900. REPA_OB_2. 1 hit. PF01336. tRNA_anti-codon. 1 hit. |
SUPFAMi | SSF50249. SSF50249. 4 hits. |
TIGRFAMsi | TIGR00617. rpa1. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q8VEE4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVGHLSEGAI EVMIQQENTS IKPILQVINI RPISTGNRSP RYRLLMSDGL
60 70 80 90 100
NTLSSFMLAT QLNTLVEGGQ LASNCVCQVH KFIVNTLKDG RKVVVLMDLE
110 120 130 140 150
VMKSAEDVGL KIGNPVPYNE GYGQQQQQQQ QQQQQAVPSP ASAATPPASK
160 170 180 190 200
PQPQNGSLGM GSTAAKAYGA SKPFGKPAGT GLLQPSGGTQ SKVVPIASLT
210 220 230 240 250
PYQSKWTICA RVTNKSQIRT WSNSRGEGKL FSLELVDESG EIRATAFNEQ
260 270 280 290 300
VDKFFPLIEV NKVYYFSKGA LKIANKQFSA VKNDYEMTFN NETSVLPCED
310 320 330 340 350
GHHLPTVQFD FTGIGDLESK AKDALVDIIG ICKSYEDSIK ITVKSNNREV
360 370 380 390 400
AKRNIYLMDM SGKVVTTTLW GEDADKFDGS RQPVMAIKGA RVSDFGGRSL
410 420 430 440 450
SVLSSSTVIV NPDIPEAYKL RGWFDSEGQA LDGVSISDHR SGGAGGGNTN
460 470 480 490 500
WKTLHEAKSE NLGQGDKADY FSTVAAVVFL RKENCMYQAC PTQDCNKKVI
510 520 530 540 550
DQQNGLYRCE KCDREFPNFK YRMILSANIA DFQENQWVTC FQESAEAILG
560 570 580 590 600
QNTMYLGELK EKNEQAFEEV FQNANFRSFT FRIRVKLETY NDESRIKATV
610 620
MDVKPVDFRD YGRRLIANIR KNM
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 134 – 135 | Missing 1 Publication | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK148035 mRNA. Translation: BAE28302.1. AK150785 mRNA. Translation: BAE29849.1. AK151590 mRNA. Translation: BAE30530.1. AK153144 mRNA. Translation: BAE31755.1. AK165316 mRNA. Translation: BAE38134.1. AK165944 mRNA. Translation: BAE38476.1. AK167598 mRNA. Translation: BAE39655.1. AK169599 mRNA. Translation: BAE41250.1. AL603834 Genomic DNA. No translation available. BC019119 mRNA. Translation: AAH19119.1. |
CCDSi | CCDS25044.1. |
RefSeqi | NP_001157695.1. NM_001164223.1. NP_080929.1. NM_026653.2. |
UniGenei | Mm.102574. Mm.180734. Mm.254027. Mm.472099. |
Genome annotation databases
Ensembli | ENSMUST00000092907; ENSMUSP00000090585; ENSMUSG00000000751. |
GeneIDi | 68275. |
KEGGi | mmu:68275. |
UCSCi | uc007kdi.2. mouse. |
Keywords - Coding sequence diversityi
PolymorphismSimilar proteinsi
Entry informationi
Entry namei | RFA1_MOUSE | |
Accessioni | Q8VEE4Primary (citable) accession number: Q8VEE4 Secondary accession number(s): Q3TEJ8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 7, 2006 |
Last sequence update: | March 1, 2002 | |
Last modified: | March 28, 2018 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |