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Protein

LIM and cysteine-rich domains protein 1

Gene

Lmcd1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional cofactor that restricts GATA6 function by inhibiting DNA-binding, resulting in repression of GATA6 transcriptional activation of downstream target genes. Represses GATA6-mediated trans activation of lung- and cardiac tissue-specific promoters. Inhibits DNA-binding by GATA4 and GATA1 to the cTNC promoter. Plays a critical role in the development of cardiac hypertrophy via activation of calcineurin/nuclear factor of activated T-cells signaling pathway.2 Publications

GO - Molecular functioni

  • transcription corepressor activity Source: UniProtKB
  • zinc ion binding Source: InterPro

GO - Biological processi

  • mitophagy in response to mitochondrial depolarization Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
  • positive regulation of defense response to virus by host Source: MGI
  • regulation of cardiac muscle hypertrophy Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
  • xenophagy Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
LIM and cysteine-rich domains protein 1
Gene namesi
Name:Lmcd1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1353635. Lmcd1.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication

  • Note: May shuttle between the cytoplasm and the nucleus.

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • extracellular matrix Source: Ensembl
  • extracellular space Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice display a marked increase in cardiac hypertrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 365365LIM and cysteine-rich domains protein 1PRO_0000075816Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei16 – 161PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VEE1.
PaxDbiQ8VEE1.
PRIDEiQ8VEE1.

PTM databases

PhosphoSiteiQ8VEE1.

Expressioni

Tissue specificityi

Expressed in distal airway epithelium of the lung, vascular smooth muscle, and myocardium.1 Publication

Developmental stagei

At embryonic day 9.5 (E9.5) expressed in the myocardium and forming anterior foregut epithelium. By E12.5, expression is observed in the airways of the developing lung as well as in the myocardium. At E14.5, when proximal-distal differentiation in the lung is proceeding rapidly, expression is observed at high levels in the distal but not proximal airways of the developing lung. By E16.5, expression decreases in airway epithelium in the lung and expression is also observed in the vascular smooth muscle of pulmonary arteries (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000057604.
CleanExiMM_LMCD1.
ExpressionAtlasiQ8VEE1. baseline and differential.
GenevisibleiQ8VEE1. MM.

Interactioni

Subunit structurei

Interacts with beta-dystroglycan (By similarity). Interacts with GATA1, GATA4 and GATA6.By similarity1 Publication

Protein-protein interaction databases

BioGridi206014. 1 interaction.
IntActiQ8VEE1. 1 interaction.
STRINGi10090.ENSMUSP00000032376.

Structurei

3D structure databases

ProteinModelPortaliQ8VEE1.
SMRiQ8VEE1. Positions 243-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 206108PETPROSITE-ProRule annotationAdd
BLAST
Domaini241 – 30666LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini307 – 36559LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 5831Cys-richAdd
BLAST

Domaini

The LIM zinc-binding domains and the Cys-rich region mediate interaction with GATA6.1 Publication

Sequence similaritiesi

Contains 2 LIM zinc-binding domains.PROSITE-ProRule annotation
Contains 1 PET domain.PROSITE-ProRule annotation

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiENOG410INGI. Eukaryota.
ENOG410ZPX3. LUCA.
GeneTreeiENSGT00550000074438.
HOGENOMiHOG000231628.
HOVERGENiHBG001038.
InParanoidiQ8VEE1.
OMAiAGYSKQW.
OrthoDBiEOG091G07C5.
PhylomeDBiQ8VEE1.
TreeFamiTF313265.

Family and domain databases

CDDicd09829. PET_testin. 1 hit.
Gene3Di2.10.110.10. 2 hits.
InterProiIPR010442. PET_domain.
IPR033724. PET_testin.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 2 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
PS51303. PET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VEE1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVAKDLNP GVQKMSLGQQ QSARGVACLR CKGMCSGFEP HSWRKICKSC
60 70 80 90 100
KCSQEEHCLS SDLDDDRKIG RLLMDSKYAT LTARVKGGDG IRIYKRNRMI
110 120 130 140 150
MTNPIATGKD PTFDTITYEW APPGVTQKLG LQYMELIPKE RQPVTGTEGA
160 170 180 190 200
LYRRRQLMHQ LPIYDQDPSR CRGLVENELK AMEEFVKHYK SEALGVGEVA
210 220 230 240 250
LPGQGGLPKE ENKTQEKPEG TETTAPTTNG SLGDPSKEVE YVCELCKGAA
260 270 280 290 300
PVDSPVVYAD RAGYSKQWHP TCFQCIKCSE PLVDLIYFWK DGAPWCGRHY
310 320 330 340 350
CESVRPRCSG CDEIIFSEDY QRVEDLAWHR KHFICEGCEQ LLSGRAYIVT
360
KGQLLCPTCS KSKRS
Length:365
Mass (Da):40,996
Last modified:March 1, 2002 - v1
Checksum:iDB8AA836364E47D3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019124 mRNA. Translation: AAH19124.1.
CCDSiCCDS20403.1.
RefSeqiNP_659048.1. NM_144799.2.
UniGeneiMm.234441.

Genome annotation databases

EnsembliENSMUST00000032376; ENSMUSP00000032376; ENSMUSG00000057604.
GeneIDi30937.
KEGGimmu:30937.
UCSCiuc009ddv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019124 mRNA. Translation: AAH19124.1.
CCDSiCCDS20403.1.
RefSeqiNP_659048.1. NM_144799.2.
UniGeneiMm.234441.

3D structure databases

ProteinModelPortaliQ8VEE1.
SMRiQ8VEE1. Positions 243-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206014. 1 interaction.
IntActiQ8VEE1. 1 interaction.
STRINGi10090.ENSMUSP00000032376.

PTM databases

PhosphoSiteiQ8VEE1.

Proteomic databases

MaxQBiQ8VEE1.
PaxDbiQ8VEE1.
PRIDEiQ8VEE1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032376; ENSMUSP00000032376; ENSMUSG00000057604.
GeneIDi30937.
KEGGimmu:30937.
UCSCiuc009ddv.2. mouse.

Organism-specific databases

CTDi29995.
MGIiMGI:1353635. Lmcd1.

Phylogenomic databases

eggNOGiENOG410INGI. Eukaryota.
ENOG410ZPX3. LUCA.
GeneTreeiENSGT00550000074438.
HOGENOMiHOG000231628.
HOVERGENiHBG001038.
InParanoidiQ8VEE1.
OMAiAGYSKQW.
OrthoDBiEOG091G07C5.
PhylomeDBiQ8VEE1.
TreeFamiTF313265.

Enzyme and pathway databases

ReactomeiR-MMU-5683826. Surfactant metabolism.

Miscellaneous databases

ChiTaRSiLmcd1. mouse.
PROiQ8VEE1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000057604.
CleanExiMM_LMCD1.
ExpressionAtlasiQ8VEE1. baseline and differential.
GenevisibleiQ8VEE1. MM.

Family and domain databases

CDDicd09829. PET_testin. 1 hit.
Gene3Di2.10.110.10. 2 hits.
InterProiIPR010442. PET_domain.
IPR033724. PET_testin.
IPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 2 hits.
PF06297. PET. 1 hit.
[Graphical view]
SMARTiSM00132. LIM. 2 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 2 hits.
PS50023. LIM_DOMAIN_2. 2 hits.
PS51303. PET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLMCD1_MOUSE
AccessioniPrimary (citable) accession number: Q8VEE1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: March 1, 2002
Last modified: September 7, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.