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Protein

Ras-related protein Rab-7b

Gene

Rab7b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls vesicular trafficking from endosomes to the trans-Golgi network (TGN). Acts as a negative regulator of TLR9 signaling and can suppress TLR9-triggered TNFA, IL6, and IFNB production in macrophages by promoting TLR9 lysosomal degradation. Also negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4. Promotes megakaryocytic differentiation by increasing NF-kappa-B-dependent IL6 production and subsequently enhancing the association of STAT3 with GATA1. Not involved in the regulation of the EGF- and EGFR degradation pathway.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTPBy similarity
Nucleotide bindingi34 – 407GTPBy similarity
Nucleotide bindingi63 – 675GTPBy similarity
Nucleotide bindingi124 – 1274GTPBy similarity
Nucleotide bindingi154 – 1552GTPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-7b
Gene namesi
Name:Rab7b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2442295. Rab7b.

Subcellular locationi

GO - Cellular componenti

  • Golgi apparatus Source: UniProtKB
  • late endosome Source: UniProtKB
  • lysosome Source: UniProtKB
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • trans-Golgi network Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Golgi apparatus, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 199199Ras-related protein Rab-7bPRO_0000121126Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei186 – 1861PhosphoserineCombined sources
Lipidationi198 – 1981S-geranylgeranyl cysteineBy similarity
Lipidationi199 – 1991S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiQ8VEA8.
PaxDbiQ8VEA8.
PRIDEiQ8VEA8.

PTM databases

iPTMnetiQ8VEA8.
PhosphoSiteiQ8VEA8.
SwissPalmiQ8VEA8.

Expressioni

Gene expression databases

BgeeiQ8VEA8.
CleanExiMM_5430435G22RIK.
ExpressionAtlasiQ8VEA8. baseline and differential.
GenevisibleiQ8VEA8. MM.

Interactioni

Protein-protein interaction databases

BioGridi230511. 1 interaction.
STRINGi10090.ENSMUSP00000065456.

Structurei

3D structure databases

ProteinModelPortaliQ8VEA8.
SMRiQ8VEA8. Positions 6-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector regionBy similarity

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ8VEA8.
KOiK07898.
OMAiYEEYQTT.
OrthoDBiEOG7VDXQK.
PhylomeDBiQ8VEA8.
TreeFamiTF326442.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VEA8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPRKKVDLK LIIVGALGVG KTSLLHQYVH KTFFEEYQTT LGASILSKII
60 70 80 90 100
ILDDTTLKLQ IWDTGGQERF RSMVSTFYKG SDGCILAFDV TDPESFEALD
110 120 130 140 150
IWRDDVLAKI IPMEQSYPMV VLGNKIDLED RKVSQEVVHG WCKEKDMPYF
160 170 180 190
EVSAKNDINV VQAFEVLASR ALLRYQGTAE NHLIDSIKLS PGQPKSRCC
Length:199
Mass (Da):22,502
Last modified:March 1, 2002 - v1
Checksum:i33353842079CF6FC
GO
Isoform 2 (identifier: Q8VEA8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     133-174: Missing.

Show »
Length:157
Mass (Da):17,712
Checksum:i7B31B49C7A169AD9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1871I → V in BAC29291 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei133 – 17442Missing in isoform 2. 1 PublicationVSP_011108Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB232641 mRNA. Translation: BAF02903.1.
AK030688 mRNA. Translation: BAC27078.1.
AK036056 mRNA. Translation: BAC29291.1.
AK079999 mRNA. Translation: BAC37802.1.
CH466520 Genomic DNA. Translation: EDL39704.1.
CCDSiCCDS15272.1. [Q8VEA8-1]
CCDS78677.1. [Q8VEA8-2]
RefSeqiNP_001298025.1. NM_001311096.1. [Q8VEA8-2]
NP_663484.1. NM_145509.3. [Q8VEA8-1]
XP_006529493.1. XM_006529430.1. [Q8VEA8-1]
UniGeneiMm.44508.

Genome annotation databases

EnsembliENSMUST00000064664; ENSMUSP00000066452; ENSMUSG00000052688. [Q8VEA8-2]
ENSMUST00000064679; ENSMUSP00000065456; ENSMUSG00000052688. [Q8VEA8-1]
GeneIDi226421.
KEGGimmu:226421.
UCSCiuc007cnp.1. mouse. [Q8VEA8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB232641 mRNA. Translation: BAF02903.1.
AK030688 mRNA. Translation: BAC27078.1.
AK036056 mRNA. Translation: BAC29291.1.
AK079999 mRNA. Translation: BAC37802.1.
CH466520 Genomic DNA. Translation: EDL39704.1.
CCDSiCCDS15272.1. [Q8VEA8-1]
CCDS78677.1. [Q8VEA8-2]
RefSeqiNP_001298025.1. NM_001311096.1. [Q8VEA8-2]
NP_663484.1. NM_145509.3. [Q8VEA8-1]
XP_006529493.1. XM_006529430.1. [Q8VEA8-1]
UniGeneiMm.44508.

3D structure databases

ProteinModelPortaliQ8VEA8.
SMRiQ8VEA8. Positions 6-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi230511. 1 interaction.
STRINGi10090.ENSMUSP00000065456.

PTM databases

iPTMnetiQ8VEA8.
PhosphoSiteiQ8VEA8.
SwissPalmiQ8VEA8.

Proteomic databases

MaxQBiQ8VEA8.
PaxDbiQ8VEA8.
PRIDEiQ8VEA8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064664; ENSMUSP00000066452; ENSMUSG00000052688. [Q8VEA8-2]
ENSMUST00000064679; ENSMUSP00000065456; ENSMUSG00000052688. [Q8VEA8-1]
GeneIDi226421.
KEGGimmu:226421.
UCSCiuc007cnp.1. mouse. [Q8VEA8-1]

Organism-specific databases

CTDi338382.
MGIiMGI:2442295. Rab7b.

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiQ8VEA8.
KOiK07898.
OMAiYEEYQTT.
OrthoDBiEOG7VDXQK.
PhylomeDBiQ8VEA8.
TreeFamiTF326442.

Miscellaneous databases

PROiQ8VEA8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VEA8.
CleanExiMM_5430435G22RIK.
ExpressionAtlasiQ8VEA8. baseline and differential.
GenevisibleiQ8VEA8. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity."
    Itoh T., Satoh M., Kanno E., Fukuda M.
    Genes Cells 11:1023-1037(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: BALB/cJ.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Blood vessel, Cerebellum and Head.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Lysosome-associated small Rab GTPase Rab7b negatively regulates TLR4 signaling in macrophages by promoting lysosomal degradation of TLR4."
    Wang Y., Chen T., Han C., He D., Liu H., An H., Cai Z., Cao X.
    Blood 110:962-971(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "Late endosome/lysosome-localized Rab7b suppresses TLR9-initiated proinflammatory cytokine and type I IFN production in macrophages."
    Yao M., Liu X., Li D., Chen T., Cai Z., Cao X.
    J. Immunol. 183:1751-1758(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.

Entry informationi

Entry nameiRAB7B_MOUSE
AccessioniPrimary (citable) accession number: Q8VEA8
Secondary accession number(s): Q0PD09, Q8BJT0, Q8BZB1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: March 1, 2002
Last modified: July 6, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.