Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8VEA6 (CRDL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chordin-like protein 2
Gene names
Name:Chrdl2
Synonyms:Chl2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length426 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in tumor angiogenesis By similarity. May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation. Ref.1 Ref.4

Subunit structure

Interacts with GDF5. May interact with INHBA, BMP2, BMP4, BMP5, BMP6, and BMP7. Ref.1

Subcellular location

Secreted By similarity.

Tissue specificity

Weakly expressed in the liver and kidney. In reproductive organs expressed in connective tissues such as ligaments of the ovary and oviduct in females, and of testis, epididymis and certain male accessory sex glands in males. Expression was high in uterine myometrium. Weakly expressed in cartilage of the femoral head, patella, articular facets of vertebrae, in the annulus fibrosus of intervertebral disks. In normal cartilage, expression was confined to articular chondrocytes especially in the superficial zone. Ref.1

Developmental stage

In embryo expressed to the surface chondrocytes of developing joint cartilage and to the connective tissue of reproductive organs. Ref.1

Sequence similarities

Contains 3 VWFC domains.

Caution

According to Ref.1 interacts with human BMP2, BMP4, BMP5, BMP6, BMP7 but not human INHBA. According to Ref.4 interacts with human INHBA but not human BMP2, BMP4 and BMP6.

Sequence caution

The sequence AAK50575.1 differs from that shown. Reason: Frameshift at position 388.

Ontologies

Keywords
   Biological processChondrogenesis
Differentiation
Osteogenesis
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
   DomainRepeat
Signal
   Molecular functionDevelopmental protein
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcartilage development

Inferred from electronic annotation. Source: UniProtKB-KW

cell differentiation

Inferred from direct assay Ref.1. Source: MGI

negative regulation of BMP signaling pathway

Inferred from direct assay Ref.1. Source: MGI

ossification

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionprotein binding

Inferred from physical interaction Ref.1. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: Additional isoforms seem to exist. Differential expression of isoforms was observed during myoblast and osteoblast differentiation and maturation.
Isoform 1 (identifier: Q8VEA6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 By similarity
Chain26 – 426401Chordin-like protein 2
PRO_0000005372

Regions

Domain31 – 9666VWFC 1
Domain109 – 17567VWFC 2
Domain246 – 31166VWFC 3

Amino acid modifications

Glycosylation1141N-linked (GlcNAc...) Potential
Glycosylation1861N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict261R → L in AAK50575. Ref.2
Sequence conflict3351H → Q in AAK50575. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 45BAB7BEE09AFE04

FASTA42647,765
        10         20         30         40         50         60 
MVPGVRIIPS LLGLVMFWLP LDSQARSRSG KVCLFGEKIY TPGQSWHPYL EPQGTIYCVR 

        70         80         90        100        110        120 
CTCSENGHVN CYRLRCPPLH CSQPVMEPQQ CCPRCVDPHV PSGLRVPLKS CQLNETTYQH 

       130        140        150        160        170        180 
GEIFSAQELF PARLSNQCVL CSCIEGHTYC GLMTCPEPSC PTTLPLPDSC CQTCKDRTTE 

       190        200        210        220        230        240 
SSTEENLTQL QHGERHSQDP CSERRGPSTP APTSLSSPLG FIPRHFQSVG MGSTTIKIIL 

       250        260        270        280        290        300 
KEKHKKACTH NGKTYSHGEV WHPTVLSFGP MPCILCTCID GYQDCHRVTC PTQYPCSQPK 

       310        320        330        340        350        360 
KVAGKCCKIC PEDEAEDDHS EVISTRCPKV PGQFHVYTLA SPSPDSLHRF VLEHEASDQV 

       370        380        390        400        410        420 
EMYIWKLVKG IYHLVQIKRV RKQDFQKEAQ NFRLLTGTHE GYWTVFLAQT PELKVTASPD 


KVTKTL 

« Hide

References

« Hide 'large scale' references
[1]"A novel chordin-like BMP inhibitor, CHL2, expressed preferentially in chondrocytes of developing cartilage and osteoarthritic joint cartilage."
Nakayama N., Han C.-Y.E., Cam L., Lee J.I., Pretorius J., Fisher S., Rosenfeld R., Scully S., Nishinakamura R., Duryea D., Van G., Bolon B., Yokota T., Zhang K.
Development 131:229-240(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH BMPS; GDF5 AND INHBA.
[2]Coffinier C.C., De Robertis E.M.
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
[4]"hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation."
Oren A., Toporik A., Biton S., Almogy N., Eshel D., Bernstein J., Savitsky K., Rotman G.
Gene 331:17-31(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ALTERNATIVE SPLICING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF338222 mRNA. Translation: AAK50575.1. Frameshift.
BC019399 mRNA. Translation: AAH19399.1.
CCDSCCDS21492.1. [Q8VEA6-1]
RefSeqNP_001278249.1. NM_001291320.1.
NP_598470.3. NM_133709.3.
UniGeneMm.285295.

3D structure databases

ProteinModelPortalQ8VEA6.
SMRQ8VEA6. Positions 31-100, 244-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid213242. 6 interactions.
STRING10090.ENSMUSP00000102699.

PTM databases

PhosphoSiteQ8VEA6.

Proteomic databases

PRIDEQ8VEA6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID69121.
KEGGmmu:69121.

Organism-specific databases

CTD25884.
MGIMGI:1916371. Chrdl2.

Phylogenomic databases

eggNOGNOG147797.
HOGENOMHOG000049211.
HOVERGENHBG051113.
KOK17280.
PhylomeDBQ8VEA6.

Gene expression databases

CleanExMM_CHRDL2.
GenevestigatorQ8VEA6.

Family and domain databases

InterProIPR001007. VWF_C.
[Graphical view]
PfamPF00093. VWC. 3 hits.
[Graphical view]
SMARTSM00214. VWC. 3 hits.
[Graphical view]
PROSITEPS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio328650.
PROQ8VEA6.
SOURCESearch...

Entry information

Entry nameCRDL2_MOUSE
AccessionPrimary (citable) accession number: Q8VEA6
Secondary accession number(s): Q925I3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot