Q8VEA6 (CRDL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified February 19, 2014. Version 80. History...
Names and origin
|Protein names||Recommended name:|
Chordin-like protein 2
|Organism||Mus musculus (Mouse) [Reference proteome]|
|Taxonomic identifier||10090 [NCBI]|
|Taxonomic lineage||Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus|
|Sequence length||426 AA.|
|Sequence processing||The displayed sequence is further processed into a mature form.|
|Protein existence||Evidence at protein level|
General annotation (Comments)
Implicated in tumor angiogenesis By similarity. May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation. Ref.1 Ref.4
Interacts with GDF5. May interact with INHBA, BMP2, BMP4, BMP5, BMP6, and BMP7. Ref.1
Secreted By similarity.
Weakly expressed in the liver and kidney. In reproductive organs expressed in connective tissues such as ligaments of the ovary and oviduct in females, and of testis, epididymis and certain male accessory sex glands in males. Expression was high in uterine myometrium. Weakly expressed in cartilage of the femoral head, patella, articular facets of vertebrae, in the annulus fibrosus of intervertebral disks. In normal cartilage, expression was confined to articular chondrocytes especially in the superficial zone. Ref.1
In embryo expressed to the surface chondrocytes of developing joint cartilage and to the connective tissue of reproductive organs. Ref.1
Contains 3 VWFC domains.
|Coding sequence diversity||Alternative splicing|
|Molecular function||Developmental protein|
|Technical term||Complete proteome|
|Gene Ontology (GO)|
Inferred from electronic annotation. Source: UniProtKB-KWcell differentiationnegative regulation of BMP signaling pathwayossification
Inferred from electronic annotation. Source: UniProtKB-KW
Inferred from electronic annotation. Source: UniProtKB-SubCell
|Complete GO annotation...|
|This entry describes 1 isoform produced by alternative splicing. [Select]|
Note: Additional isoforms seem to exist. Differential expression of isoforms was observed during myoblast and osteoblast differentiation and maturation.
|Isoform 1 (identifier: Q8VEA6-1) |
This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Sequence annotation (Features)
|Feature key||Position(s)||Length||Description||Graphical view||Feature identifier|
|Signal peptide||1 – 25||25||By similarity|
|Chain||26 – 426||401||Chordin-like protein 2||PRO_0000005372|
|Domain||31 – 96||66||VWFC 1|
|Domain||109 – 175||67||VWFC 2|
|Domain||246 – 311||66||VWFC 3|
Amino acid modifications
|Glycosylation||114||1||N-linked (GlcNAc...) Potential|
|Glycosylation||186||1||N-linked (GlcNAc...) Potential|
|Sequence conflict||26||1||R → L in AAK50575. Ref.2|
|Sequence conflict||335||1||H → Q in AAK50575. Ref.2|
|||"A novel chordin-like BMP inhibitor, CHL2, expressed preferentially in chondrocytes of developing cartilage and osteoarthritic joint cartilage."|
Nakayama N., Han C.-Y.E., Cam L., Lee J.I., Pretorius J., Fisher S., Rosenfeld R., Scully S., Nishinakamura R., Duryea D., Van G., Bolon B., Yokota T., Zhang K.
Development 131:229-240(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH BMPS; GDF5 AND INHBA.
|||Coffinier C.C., De Robertis E.M.|
Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
|||"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."|
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Mammary tumor.
|||"hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation."|
Oren A., Toporik A., Biton S., Almogy N., Eshel D., Bernstein J., Savitsky K., Rotman G.
Gene 331:17-31(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ALTERNATIVE SPLICING.
|+||Additional computationally mapped references.|
|AF338222 mRNA. Translation: AAK50575.1. Frameshift.|
BC019399 mRNA. Translation: AAH19399.1.
|RefSeq||NP_598470.3. NM_133709.3. |
3D structure databases
|SMR||Q8VEA6. Positions 31-100, 244-316. |
Protein-protein interaction databases
|BioGrid||213242. 6 interactions.|
Protocols and materials databases
Genome annotation databases
|MGI||MGI:1916371. Chrdl2. |
Gene expression databases
Family and domain databases
|InterPro||IPR001007. VWF_C. |
|Pfam||PF00093. VWC. 3 hits. |
|SMART||SM00214. VWC. 3 hits. |
|PROSITE||PS01208. VWFC_1. 3 hits. |
PS50184. VWFC_2. 3 hits.
|Accession||Primary (citable) accession number: Q8VEA6|
Secondary accession number(s): Q925I3
|Entry status||Reviewed (UniProtKB/Swiss-Prot)|
|Annotation program||Chordata Protein Annotation Program|