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Q8VEA6

- CRDL2_MOUSE

UniProt

Q8VEA6 - CRDL2_MOUSE

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Protein

Chordin-like protein 2

Gene

Chrdl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Implicated in tumor angiogenesis (By similarity). May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation.By similarity2 Publications

GO - Biological processi

  1. cartilage development Source: UniProtKB-KW
  2. cell differentiation Source: MGI
  3. negative regulation of BMP signaling pathway Source: MGI
  4. ossification Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Chondrogenesis, Differentiation, Osteogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Chordin-like protein 2
Gene namesi
Name:Chrdl2
Synonyms:Chl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1916371. Chrdl2.

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525By similarityAdd
BLAST
Chaini26 – 426401Chordin-like protein 2PRO_0000005372Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ8VEA6.

PTM databases

PhosphoSiteiQ8VEA6.

Expressioni

Tissue specificityi

Weakly expressed in the liver and kidney. In reproductive organs expressed in connective tissues such as ligaments of the ovary and oviduct in females, and of testis, epididymis and certain male accessory sex glands in males. Expression was high in uterine myometrium. Weakly expressed in cartilage of the femoral head, patella, articular facets of vertebrae, in the annulus fibrosus of intervertebral disks. In normal cartilage, expression was confined to articular chondrocytes especially in the superficial zone.1 Publication

Developmental stagei

In embryo expressed to the surface chondrocytes of developing joint cartilage and to the connective tissue of reproductive organs.1 Publication

Gene expression databases

CleanExiMM_CHRDL2.
GenevestigatoriQ8VEA6.

Interactioni

Subunit structurei

Interacts with GDF5. May interact with INHBA, BMP2, BMP4, BMP5, BMP6, and BMP7.1 Publication

Protein-protein interaction databases

BioGridi213242. 6 interactions.
STRINGi10090.ENSMUSP00000102699.

Structurei

3D structure databases

ProteinModelPortaliQ8VEA6.
SMRiQ8VEA6. Positions 33-76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini31 – 9666VWFC 1PROSITE-ProRule annotationAdd
BLAST
Domaini109 – 17567VWFC 2PROSITE-ProRule annotationAdd
BLAST
Domaini246 – 31166VWFC 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 VWFC domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiNOG147797.
HOGENOMiHOG000049211.
HOVERGENiHBG051113.
InParanoidiQ8VEA6.
KOiK17280.
PhylomeDBiQ8VEA6.

Family and domain databases

InterProiIPR001007. VWF_C.
[Graphical view]
PfamiPF00093. VWC. 3 hits.
[Graphical view]
SMARTiSM00214. VWC. 3 hits.
[Graphical view]
PROSITEiPS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: Additional isoforms seem to exist. Differential expression of isoforms was observed during myoblast and osteoblast differentiation and maturation.

Isoform 1 (identifier: Q8VEA6-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPGVRIIPS LLGLVMFWLP LDSQARSRSG KVCLFGEKIY TPGQSWHPYL
60 70 80 90 100
EPQGTIYCVR CTCSENGHVN CYRLRCPPLH CSQPVMEPQQ CCPRCVDPHV
110 120 130 140 150
PSGLRVPLKS CQLNETTYQH GEIFSAQELF PARLSNQCVL CSCIEGHTYC
160 170 180 190 200
GLMTCPEPSC PTTLPLPDSC CQTCKDRTTE SSTEENLTQL QHGERHSQDP
210 220 230 240 250
CSERRGPSTP APTSLSSPLG FIPRHFQSVG MGSTTIKIIL KEKHKKACTH
260 270 280 290 300
NGKTYSHGEV WHPTVLSFGP MPCILCTCID GYQDCHRVTC PTQYPCSQPK
310 320 330 340 350
KVAGKCCKIC PEDEAEDDHS EVISTRCPKV PGQFHVYTLA SPSPDSLHRF
360 370 380 390 400
VLEHEASDQV EMYIWKLVKG IYHLVQIKRV RKQDFQKEAQ NFRLLTGTHE
410 420
GYWTVFLAQT PELKVTASPD KVTKTL
Length:426
Mass (Da):47,765
Last modified:March 1, 2002 - v1
Checksum:i45BAB7BEE09AFE04
GO

Sequence cautioni

The sequence AAK50575.1 differs from that shown. Reason: Frameshift at position 388.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261R → L in AAK50575. 1 PublicationCurated
Sequence conflicti335 – 3351H → Q in AAK50575. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF338222 mRNA. Translation: AAK50575.1. Frameshift.
BC019399 mRNA. Translation: AAH19399.1.
CCDSiCCDS21492.1. [Q8VEA6-1]
RefSeqiNP_001278249.1. NM_001291320.1.
NP_598470.3. NM_133709.3.
UniGeneiMm.285295.

Genome annotation databases

GeneIDi69121.
KEGGimmu:69121.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF338222 mRNA. Translation: AAK50575.1 . Frameshift.
BC019399 mRNA. Translation: AAH19399.1 .
CCDSi CCDS21492.1. [Q8VEA6-1 ]
RefSeqi NP_001278249.1. NM_001291320.1.
NP_598470.3. NM_133709.3.
UniGenei Mm.285295.

3D structure databases

ProteinModelPortali Q8VEA6.
SMRi Q8VEA6. Positions 33-76.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 213242. 6 interactions.
STRINGi 10090.ENSMUSP00000102699.

PTM databases

PhosphoSitei Q8VEA6.

Proteomic databases

PRIDEi Q8VEA6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 69121.
KEGGi mmu:69121.

Organism-specific databases

CTDi 25884.
MGIi MGI:1916371. Chrdl2.

Phylogenomic databases

eggNOGi NOG147797.
HOGENOMi HOG000049211.
HOVERGENi HBG051113.
InParanoidi Q8VEA6.
KOi K17280.
PhylomeDBi Q8VEA6.

Miscellaneous databases

NextBioi 328650.
PROi Q8VEA6.
SOURCEi Search...

Gene expression databases

CleanExi MM_CHRDL2.
Genevestigatori Q8VEA6.

Family and domain databases

InterProi IPR001007. VWF_C.
[Graphical view ]
Pfami PF00093. VWC. 3 hits.
[Graphical view ]
SMARTi SM00214. VWC. 3 hits.
[Graphical view ]
PROSITEi PS01208. VWFC_1. 3 hits.
PS50184. VWFC_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel chordin-like BMP inhibitor, CHL2, expressed preferentially in chondrocytes of developing cartilage and osteoarthritic joint cartilage."
    Nakayama N., Han C.-Y.E., Cam L., Lee J.I., Pretorius J., Fisher S., Rosenfeld R., Scully S., Nishinakamura R., Duryea D., Van G., Bolon B., Yokota T., Zhang K.
    Development 131:229-240(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH BMPS; GDF5 AND INHBA.
  2. Coffinier C.C., De Robertis E.M.
    Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  4. "hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation."
    Oren A., Toporik A., Biton S., Almogy N., Eshel D., Bernstein J., Savitsky K., Rotman G.
    Gene 331:17-31(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ALTERNATIVE SPLICING.

Entry informationi

Entry nameiCRDL2_MOUSE
AccessioniPrimary (citable) accession number: Q8VEA6
Secondary accession number(s): Q925I3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

According to PubMed:14660436 interacts with human BMP2, BMP4, BMP5, BMP6, BMP7 but not human INHBA. According to PubMed:15094188 interacts with human INHBA but not human BMP2, BMP4 and BMP6.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3