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Q8VEA6

- CRDL2_MOUSE

UniProt

Q8VEA6 - CRDL2_MOUSE

Protein

Chordin-like protein 2

Gene

Chrdl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Implicated in tumor angiogenesis By similarity. May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation.By similarity2 Publications

    GO - Molecular functioni

    1. protein binding Source: MGI

    GO - Biological processi

    1. cartilage development Source: UniProtKB-KW
    2. cell differentiation Source: MGI
    3. negative regulation of BMP signaling pathway Source: MGI
    4. ossification Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Chondrogenesis, Differentiation, Osteogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Chordin-like protein 2
    Gene namesi
    Name:Chrdl2
    Synonyms:Chl2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1916371. Chrdl2.

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525By similarityAdd
    BLAST
    Chaini26 – 426401Chordin-like protein 2PRO_0000005372Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi186 – 1861N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ8VEA6.

    PTM databases

    PhosphoSiteiQ8VEA6.

    Expressioni

    Tissue specificityi

    Weakly expressed in the liver and kidney. In reproductive organs expressed in connective tissues such as ligaments of the ovary and oviduct in females, and of testis, epididymis and certain male accessory sex glands in males. Expression was high in uterine myometrium. Weakly expressed in cartilage of the femoral head, patella, articular facets of vertebrae, in the annulus fibrosus of intervertebral disks. In normal cartilage, expression was confined to articular chondrocytes especially in the superficial zone.1 Publication

    Developmental stagei

    In embryo expressed to the surface chondrocytes of developing joint cartilage and to the connective tissue of reproductive organs.1 Publication

    Gene expression databases

    CleanExiMM_CHRDL2.
    GenevestigatoriQ8VEA6.

    Interactioni

    Subunit structurei

    Interacts with GDF5. May interact with INHBA, BMP2, BMP4, BMP5, BMP6, and BMP7.1 Publication

    Protein-protein interaction databases

    BioGridi213242. 6 interactions.
    STRINGi10090.ENSMUSP00000102699.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VEA6.
    SMRiQ8VEA6. Positions 31-100, 244-316.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini31 – 9666VWFC 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini109 – 17567VWFC 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini246 – 31166VWFC 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 VWFC domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    eggNOGiNOG147797.
    HOGENOMiHOG000049211.
    HOVERGENiHBG051113.
    KOiK17280.
    PhylomeDBiQ8VEA6.

    Family and domain databases

    InterProiIPR001007. VWF_C.
    [Graphical view]
    PfamiPF00093. VWC. 3 hits.
    [Graphical view]
    SMARTiSM00214. VWC. 3 hits.
    [Graphical view]
    PROSITEiPS01208. VWFC_1. 3 hits.
    PS50184. VWFC_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: Additional isoforms seem to exist. Differential expression of isoforms was observed during myoblast and osteoblast differentiation and maturation.

    Isoform 1 (identifier: Q8VEA6-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVPGVRIIPS LLGLVMFWLP LDSQARSRSG KVCLFGEKIY TPGQSWHPYL    50
    EPQGTIYCVR CTCSENGHVN CYRLRCPPLH CSQPVMEPQQ CCPRCVDPHV 100
    PSGLRVPLKS CQLNETTYQH GEIFSAQELF PARLSNQCVL CSCIEGHTYC 150
    GLMTCPEPSC PTTLPLPDSC CQTCKDRTTE SSTEENLTQL QHGERHSQDP 200
    CSERRGPSTP APTSLSSPLG FIPRHFQSVG MGSTTIKIIL KEKHKKACTH 250
    NGKTYSHGEV WHPTVLSFGP MPCILCTCID GYQDCHRVTC PTQYPCSQPK 300
    KVAGKCCKIC PEDEAEDDHS EVISTRCPKV PGQFHVYTLA SPSPDSLHRF 350
    VLEHEASDQV EMYIWKLVKG IYHLVQIKRV RKQDFQKEAQ NFRLLTGTHE 400
    GYWTVFLAQT PELKVTASPD KVTKTL 426
    Length:426
    Mass (Da):47,765
    Last modified:March 1, 2002 - v1
    Checksum:i45BAB7BEE09AFE04
    GO

    Sequence cautioni

    The sequence AAK50575.1 differs from that shown. Reason: Frameshift at position 388.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261R → L in AAK50575. 1 PublicationCurated
    Sequence conflicti335 – 3351H → Q in AAK50575. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF338222 mRNA. Translation: AAK50575.1. Frameshift.
    BC019399 mRNA. Translation: AAH19399.1.
    CCDSiCCDS21492.1. [Q8VEA6-1]
    RefSeqiNP_001278249.1. NM_001291320.1.
    NP_598470.3. NM_133709.3.
    UniGeneiMm.285295.

    Genome annotation databases

    GeneIDi69121.
    KEGGimmu:69121.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF338222 mRNA. Translation: AAK50575.1 . Frameshift.
    BC019399 mRNA. Translation: AAH19399.1 .
    CCDSi CCDS21492.1. [Q8VEA6-1 ]
    RefSeqi NP_001278249.1. NM_001291320.1.
    NP_598470.3. NM_133709.3.
    UniGenei Mm.285295.

    3D structure databases

    ProteinModelPortali Q8VEA6.
    SMRi Q8VEA6. Positions 31-100, 244-316.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 213242. 6 interactions.
    STRINGi 10090.ENSMUSP00000102699.

    PTM databases

    PhosphoSitei Q8VEA6.

    Proteomic databases

    PRIDEi Q8VEA6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 69121.
    KEGGi mmu:69121.

    Organism-specific databases

    CTDi 25884.
    MGIi MGI:1916371. Chrdl2.

    Phylogenomic databases

    eggNOGi NOG147797.
    HOGENOMi HOG000049211.
    HOVERGENi HBG051113.
    KOi K17280.
    PhylomeDBi Q8VEA6.

    Miscellaneous databases

    NextBioi 328650.
    PROi Q8VEA6.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_CHRDL2.
    Genevestigatori Q8VEA6.

    Family and domain databases

    InterProi IPR001007. VWF_C.
    [Graphical view ]
    Pfami PF00093. VWC. 3 hits.
    [Graphical view ]
    SMARTi SM00214. VWC. 3 hits.
    [Graphical view ]
    PROSITEi PS01208. VWFC_1. 3 hits.
    PS50184. VWFC_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel chordin-like BMP inhibitor, CHL2, expressed preferentially in chondrocytes of developing cartilage and osteoarthritic joint cartilage."
      Nakayama N., Han C.-Y.E., Cam L., Lee J.I., Pretorius J., Fisher S., Rosenfeld R., Scully S., Nishinakamura R., Duryea D., Van G., Bolon B., Yokota T., Zhang K.
      Development 131:229-240(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH BMPS; GDF5 AND INHBA.
    2. Coffinier C.C., De Robertis E.M.
      Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.
    4. "hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation."
      Oren A., Toporik A., Biton S., Almogy N., Eshel D., Bernstein J., Savitsky K., Rotman G.
      Gene 331:17-31(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ALTERNATIVE SPLICING.

    Entry informationi

    Entry nameiCRDL2_MOUSE
    AccessioniPrimary (citable) accession number: Q8VEA6
    Secondary accession number(s): Q925I3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Caution

    According to PubMed:14660436 interacts with human BMP2, BMP4, BMP5, BMP6, BMP7 but not human INHBA. According to PubMed:15094188 interacts with human INHBA but not human BMP2, BMP4 and BMP6.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3