Q8VEA6 (CRDL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chordin-like protein 2

Gene names

Name:	Chrdl2
Synonyms:	Chl2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	426 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in tumor angiogenesis By similarity. May inhibits BMPs activity by blocking their interaction with their receptors. Has a negative regulator effect on the cartilage formation/regeneration from immature mesenchymal cells, by preventing or reducing the rate of matrix accumulation. May play a role during myoblast and osteoblast differentiation, and maturation. Ref.1 Ref.4
Subunit structure	Interacts with GDF5. May interact with INHBA, BMP2, BMP4, BMP5, BMP6, and BMP7. Ref.1
Subcellular location	Secreted By similarity.
Tissue specificity	Weakly expressed in the liver and kidney. In reproductive organs expressed in connective tissues such as ligaments of the ovary and oviduct in females, and of testis, epididymis and certain male accessory sex glands in males. Expression was high in uterine myometrium. Weakly expressed in cartilage of the femoral head, patella, articular facets of vertebrae, in the annulus fibrosus of intervertebral disks. In normal cartilage, expression was confined to articular chondrocytes especially in the superficial zone. Ref.1
Developmental stage	In embryo expressed to the surface chondrocytes of developing joint cartilage and to the connective tissue of reproductive organs. Ref.1
Sequence similarities	Contains 3 VWFC domains.
Caution	According to Ref.1 interacts with human BMP2, BMP4, BMP5, BMP6, BMP7 but not human INHBA. According to Ref.4 interacts with human INHBA but not human BMP2, BMP4 and BMP6.
Sequence caution	The sequence AAK50575.1 differs from that shown. Reason: Frameshift at position 388.

Ontologies

Keywords
Biological process	Chondrogenesis Differentiation Osteogenesis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Repeat Signal
Molecular function	Developmental protein
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cartilage development Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from direct assay Ref.1. Source: MGI negative regulation of BMP signaling pathway Inferred from direct assay Ref.1. Source: MGI ossification Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: Additional isoforms seem to exist. Differential expression of isoforms was observed during myoblast and osteoblast differentiation and maturation.

Isoform 1 (identifier: Q8VEA6-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	By similarity
	Chain	26 – 426	401	Chordin-like protein 2		PRO_0000005372
Regions
	Domain	31 – 96	66	VWFC 1
	Domain	109 – 175	67	VWFC 2
	Domain	246 – 311	66	VWFC 3
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	26	1	R → L in AAK50575. Ref.2
	Sequence conflict	335	1	H → Q in AAK50575. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 45BAB7BEE09AFE04	FASTA	426	47,765
	10 20 30 40 50 60 MVPGVRIIPS LLGLVMFWLP LDSQARSRSG KVCLFGEKIY TPGQSWHPYL EPQGTIYCVR 70 80 90 100 110 120 CTCSENGHVN CYRLRCPPLH CSQPVMEPQQ CCPRCVDPHV PSGLRVPLKS CQLNETTYQH 130 140 150 160 170 180 GEIFSAQELF PARLSNQCVL CSCIEGHTYC GLMTCPEPSC PTTLPLPDSC CQTCKDRTTE 190 200 210 220 230 240 SSTEENLTQL QHGERHSQDP CSERRGPSTP APTSLSSPLG FIPRHFQSVG MGSTTIKIIL 250 260 270 280 290 300 KEKHKKACTH NGKTYSHGEV WHPTVLSFGP MPCILCTCID GYQDCHRVTC PTQYPCSQPK 310 320 330 340 350 360 KVAGKCCKIC PEDEAEDDHS EVISTRCPKV PGQFHVYTLA SPSPDSLHRF VLEHEASDQV 370 380 390 400 410 420 EMYIWKLVKG IYHLVQIKRV RKQDFQKEAQ NFRLLTGTHE GYWTVFLAQT PELKVTASPD KVTKTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel chordin-like BMP inhibitor, CHL2, expressed preferentially in chondrocytes of developing cartilage and osteoarthritic joint cartilage." Nakayama N., Han C.-Y.E., Cam L., Lee J.I., Pretorius J., Fisher S., Rosenfeld R., Scully S., Nishinakamura R., Duryea D., Van G., Bolon B., Yokota T., Zhang K. Development 131:229-240(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INTERACTION WITH BMPS; GDF5 AND INHBA.
[2]	Coffinier C.C., De Robertis E.M. Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary tumor.
[4]	"hCHL2, a novel chordin-related gene, displays differential expression and complex alternative splicing in human tissues and during myoblast and osteoblast maturation." Oren A., Toporik A., Biton S., Almogy N., Eshel D., Bernstein J., Savitsky K., Rotman G. Gene 331:17-31(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ALTERNATIVE SPLICING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF338222 mRNA. Translation: AAK50575.1. Frameshift. BC019399 mRNA. Translation: AAH19399.1.
IPI	IPI00312036.
RefSeq	NP_598470.3. NM_133709.3.
UniGene	Mm.285295.
3D structure databases
ProteinModelPortal	Q8VEA6.
SMR	Q8VEA6. Positions 30-100, 245-316.
ModBase	Search...
PTM databases
PhosphoSite	Q8VEA6.
Proteomic databases
PRIDE	Q8VEA6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	69121.
KEGG	mmu:69121.
Organism-specific databases
CTD	25884.
MGI	MGI:1916371. Chrdl2.
Phylogenomic databases
eggNOG	NOG147797.
HOGENOM	HOG000049211.
HOVERGEN	HBG051113.
OrthoDB	EOG4MCX0H.
Gene expression databases
CleanEx	MM_CHRDL2.
Genevestigator	Q8VEA6.
GermOnline	ENSMUSG00000030732. Mus musculus.
Family and domain databases
InterPro	IPR001007. VWF_C. [Graphical view]
Pfam	PF00093. VWC. 3 hits. [Graphical view]
SMART	SM00214. VWC. 3 hits. [Graphical view]
PROSITE	PS01208. VWFC_1. 3 hits. PS50184. VWFC_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	328650.
SOURCE	Search...

Entry information

Entry name

CRDL2_MOUSE

Accession

Primary (citable) accession number: Q8VEA6
Secondary accession number(s): Q925I3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program