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Protein

CD276 antigen

Gene

Cd276

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.5 Publications

GO - Molecular functioni

  • receptor binding Source: MGI

GO - Biological processi

  • negative regulation of inflammatory response Source: MGI
  • negative regulation of interferon-gamma biosynthetic process Source: MGI
  • negative regulation of interleukin-2 biosynthetic process Source: MGI
  • negative regulation of T cell proliferation Source: MGI
  • positive regulation of bone mineralization Source: MGI
  • positive regulation of interferon-gamma biosynthetic process Source: MGI
  • positive regulation of interleukin-2 secretion Source: MGI
  • positive regulation of osteoblast differentiation Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • regulation of immune response Source: MGI
  • T cell activation Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
CD276 antigen
Alternative name(s):
B7 homolog 3
Short name:
B7-H3
Costimulatory molecule
CD_antigen: CD276
Gene namesi
Name:Cd276
Synonyms:B7h3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2183926. Cd276.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 248ExtracellularSequence analysisAdd BLAST220
Transmembranei249 – 269HelicalSequence analysisAdd BLAST21
Topological domaini270 – 316CytoplasmicSequence analysisAdd BLAST47

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display a lower bone mineral density in cortical bones with femurs more susceptible to bone fracture, but do not exhibit any important skeletal abnormalities. Calvarial cells reveal normal number of osteoblast precursor cells with adequate proliferative capacity, but have impaired osteogenic differentiation. Furthermore, following cardiac transplantation, they display permanent survival under rapamycin regimen and cardiac transplants also show markedly decreased production of key cytokine and chemokine. The incidence of chronic transplant rejection is also inhibited. Mice also develop more severe airway inflammation and experimental autoimmune encephalomyelitis earlier than wild-type littermates as well as accumulate higher concentrations of autoantibodies to DNA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000004580229 – 316CD276 antigenAdd BLAST288

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi104N-linked (GlcNAc...)2 Publications1
Disulfide bondi165 ↔ 220PROSITE-ProRule annotation
Glycosylationi189N-linked (GlcNAc...)2 Publications1
Glycosylationi197N-linked (GlcNAc...); atypical1 Publication1
Glycosylationi215N-linked (GlcNAc...)1 Publication1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ8VE98.
PaxDbiQ8VE98.
PeptideAtlasiQ8VE98.
PRIDEiQ8VE98.

PTM databases

PhosphoSitePlusiQ8VE98.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Developmental stagei

Highly expressed in developing bones during embryogenesis and expression increases as osteoblast precursor cells differentiate into mature osteoblasts.1 Publication

Inductioni

Up-regulated in cells mediating rejection of mouse transplant.1 Publication

Gene expression databases

BgeeiENSMUSG00000035914.
CleanExiMM_CD276.
ExpressionAtlasiQ8VE98. baseline and differential.
GenevisibleiQ8VE98. MM.

Interactioni

Subunit structurei

Interacts with TREML2 and this interaction enhances T-cell activation.By similarity

GO - Molecular functioni

  • receptor binding Source: MGI

Protein-protein interaction databases

DIPiDIP-60156N.
STRINGi10090.ENSMUSP00000042681.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi46 – 48Combined sources3
Turni60 – 62Combined sources3
Beta strandi63 – 69Combined sources7
Turni70 – 72Combined sources3
Beta strandi75 – 80Combined sources6
Beta strandi83 – 89Combined sources7
Turni97 – 99Combined sources3
Helixi100 – 102Combined sources3
Beta strandi107 – 109Combined sources3
Helixi114 – 116Combined sources3
Beta strandi120 – 126Combined sources7
Beta strandi146 – 148Combined sources3
Beta strandi159 – 172Combined sources14
Beta strandi175 – 179Combined sources5
Beta strandi188 – 196Combined sources9
Beta strandi202 – 211Combined sources10
Beta strandi217 – 224Combined sources8
Turni225 – 228Combined sources4
Beta strandi229 – 236Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I0KX-ray2.97A34-247[»]
ProteinModelPortaliQ8VE98.
SMRiQ8VE98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 139Ig-like V-typeAdd BLAST111
Domaini145 – 238Ig-like C2-typeAdd BLAST94

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IR2H. Eukaryota.
ENOG410ZB69. LUCA.
GeneTreeiENSGT00700000104325.
HOGENOMiHOG000293131.
HOVERGENiHBG079786.
InParanoidiQ8VE98.
KOiK06746.
OMAiCRRKIKE.
OrthoDBiEOG091G0IEZ.
PhylomeDBiQ8VE98.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VE98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRGWGGPSV GVCVRTALGV LCLCLTGAVE VQVSEDPVVA LVDTDATLRC
60 70 80 90 100
SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYS NRTALFPDLL
110 120 130 140 150
VQGNASLRLQ RVRVTDEGSY TCFVSIQDFD SAAVSLQVAA PYSKPSMTLE
160 170 180 190 200
PNKDLRPGNM VTITCSSYQG YPEAEVFWKD GQGVPLTGNV TTSQMANERG
210 220 230 240 250
LFDVHSVLRV VLGANGTYSC LVRNPVLQQD AHGSVTITGQ PLTFPPEALW
260 270 280 290 300
VTVGLSVCLV VLLVALAFVC WRKIKQSCEE ENAGAEDQDG DGEGSKTALR
310
PLKPSENKED DGQEIA
Length:316
Mass (Da):34,001
Last modified:March 1, 2002 - v1
Checksum:i7BA30B1E67F55827
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY190318 mRNA. Translation: AAP04007.1.
AK155114 mRNA. Translation: BAE33058.1.
BC019436 mRNA. Translation: AAH19436.1.
BC056608 mRNA. Translation: AAH56608.1.
CCDSiCCDS23244.1.
RefSeqiNP_598744.1. NM_133983.4.
UniGeneiMm.5356.

Genome annotation databases

EnsembliENSMUST00000039788; ENSMUSP00000042681; ENSMUSG00000035914.
ENSMUST00000165365; ENSMUSP00000129418; ENSMUSG00000035914.
GeneIDi102657.
KEGGimmu:102657.
UCSCiuc009pxb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY190318 mRNA. Translation: AAP04007.1.
AK155114 mRNA. Translation: BAE33058.1.
BC019436 mRNA. Translation: AAH19436.1.
BC056608 mRNA. Translation: AAH56608.1.
CCDSiCCDS23244.1.
RefSeqiNP_598744.1. NM_133983.4.
UniGeneiMm.5356.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4I0KX-ray2.97A34-247[»]
ProteinModelPortaliQ8VE98.
SMRiQ8VE98.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60156N.
STRINGi10090.ENSMUSP00000042681.

PTM databases

PhosphoSitePlusiQ8VE98.

Proteomic databases

MaxQBiQ8VE98.
PaxDbiQ8VE98.
PeptideAtlasiQ8VE98.
PRIDEiQ8VE98.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000039788; ENSMUSP00000042681; ENSMUSG00000035914.
ENSMUST00000165365; ENSMUSP00000129418; ENSMUSG00000035914.
GeneIDi102657.
KEGGimmu:102657.
UCSCiuc009pxb.1. mouse.

Organism-specific databases

CTDi80381.
MGIiMGI:2183926. Cd276.

Phylogenomic databases

eggNOGiENOG410IR2H. Eukaryota.
ENOG410ZB69. LUCA.
GeneTreeiENSGT00700000104325.
HOGENOMiHOG000293131.
HOVERGENiHBG079786.
InParanoidiQ8VE98.
KOiK06746.
OMAiCRRKIKE.
OrthoDBiEOG091G0IEZ.
PhylomeDBiQ8VE98.
TreeFamiTF331083.

Miscellaneous databases

PROiQ8VE98.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000035914.
CleanExiMM_CD276.
ExpressionAtlasiQ8VE98. baseline and differential.
GenevisibleiQ8VE98. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR003598. Ig_sub2.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 2 hits.
SM00408. IGc2. 2 hits.
SM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 2 hits.
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD276_MOUSE
AccessioniPrimary (citable) accession number: Q8VE98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Gene transfer of B7-H3 leads to complete regression of 50 per cent tumors, or significantly slows tumor growth.
In primates, B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.