Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8VE98

- CD276_MOUSE

UniProt

Q8VE98 - CD276_MOUSE

Protein

CD276 antigen

Gene

Cd276

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.5 Publications

    GO - Molecular functioni

    1. receptor binding Source: MGI

    GO - Biological processi

    1. negative regulation of inflammatory response Source: MGI
    2. negative regulation of interferon-gamma biosynthetic process Source: MGI
    3. negative regulation of interleukin-2 biosynthetic process Source: MGI
    4. negative regulation of T cell proliferation Source: MGI
    5. positive regulation of bone mineralization Source: MGI
    6. positive regulation of interferon-gamma biosynthetic process Source: Ensembl
    7. positive regulation of interleukin-2 secretion Source: MGI
    8. positive regulation of osteoblast differentiation Source: MGI
    9. positive regulation of T cell proliferation Source: MGI
    10. regulation of immune response Source: MGI
    11. T cell activation Source: Ensembl

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD276 antigen
    Alternative name(s):
    B7 homolog 3
    Short name:
    B7-H3
    Costimulatory molecule
    CD_antigen: CD276
    Gene namesi
    Name:Cd276
    Synonyms:B7h3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:2183926. Cd276.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: MGI
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice display a lower bone mineral density in cortical bones with femurs more susceptible to bone fracture, but do not exhibit any important skeletal abnormalities. Calvarial cells reveal normal number of osteoblast precursor cells with adequate proliferative capacity, but have impaired osteogenic differentiation. Furthermore, following cardiac transplantation, they display permanent survival under rapamycin regimen and cardiac transplants also show markedly decreased production of key cytokine and chemokine. The incidence of chronic transplant rejection is also inhibited. Mice also develop more severe airway inflammation and experimental autoimmune encephalomyelitis earlier than wild-type littermates as well as accumulate higher concentrations of autoantibodies to DNA.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 316288CD276 antigenPRO_0000045802Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi104 – 1041N-linked (GlcNAc...)2 Publications
    Disulfide bondi165 ↔ 220PROSITE-ProRule annotation
    Glycosylationi189 – 1891N-linked (GlcNAc...)2 Publications
    Glycosylationi197 – 1971N-linked (GlcNAc...); atypical1 Publication
    Glycosylationi215 – 2151N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ8VE98.

    PTM databases

    PhosphoSiteiQ8VE98.

    Expressioni

    Tissue specificityi

    Ubiquitous.1 Publication

    Developmental stagei

    Highly expressed in developing bones during embryogenesis and expression increases as osteoblast precursor cells differentiate into mature osteoblasts.1 Publication

    Inductioni

    Up-regulated in cells mediating rejection of mouse transplant.1 Publication

    Gene expression databases

    ArrayExpressiQ8VE98.
    BgeeiQ8VE98.
    CleanExiMM_CD276.
    GenevestigatoriQ8VE98.

    Interactioni

    Subunit structurei

    Interacts with TREML2 and this interaction enhances T-cell activation.By similarity

    Protein-protein interaction databases

    DIPiDIP-60156N.

    Structurei

    Secondary structure

    1
    316
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi46 – 483
    Turni60 – 623
    Beta strandi63 – 697
    Turni70 – 723
    Beta strandi75 – 806
    Beta strandi83 – 897
    Turni97 – 993
    Helixi100 – 1023
    Beta strandi107 – 1093
    Helixi114 – 1163
    Beta strandi120 – 1267
    Beta strandi146 – 1483
    Beta strandi159 – 17214
    Beta strandi175 – 1795
    Beta strandi188 – 1969
    Beta strandi202 – 21110
    Beta strandi217 – 2248
    Turni225 – 2284
    Beta strandi229 – 2368

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4I0KX-ray2.97A34-247[»]
    ProteinModelPortaliQ8VE98.
    SMRiQ8VE98. Positions 35-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 248220ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini270 – 31647CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei249 – 26921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini29 – 139111Ig-like V-typeAdd
    BLAST
    Domaini145 – 23894Ig-like C2-typeAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG85729.
    GeneTreeiENSGT00700000104325.
    HOGENOMiHOG000293131.
    HOVERGENiHBG079786.
    InParanoidiQ8VE98.
    KOiK06746.
    OMAiETHASVT.
    OrthoDBiEOG7FBRH9.
    PhylomeDBiQ8VE98.
    TreeFamiTF331083.

    Family and domain databases

    Gene3Di2.60.40.10. 2 hits.
    InterProiIPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view]
    PfamiPF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VE98-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRGWGGPSV GVCVRTALGV LCLCLTGAVE VQVSEDPVVA LVDTDATLRC    50
    SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYS NRTALFPDLL 100
    VQGNASLRLQ RVRVTDEGSY TCFVSIQDFD SAAVSLQVAA PYSKPSMTLE 150
    PNKDLRPGNM VTITCSSYQG YPEAEVFWKD GQGVPLTGNV TTSQMANERG 200
    LFDVHSVLRV VLGANGTYSC LVRNPVLQQD AHGSVTITGQ PLTFPPEALW 250
    VTVGLSVCLV VLLVALAFVC WRKIKQSCEE ENAGAEDQDG DGEGSKTALR 300
    PLKPSENKED DGQEIA 316
    Length:316
    Mass (Da):34,001
    Last modified:March 1, 2002 - v1
    Checksum:i7BA30B1E67F55827
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY190318 mRNA. Translation: AAP04007.1.
    AK155114 mRNA. Translation: BAE33058.1.
    BC019436 mRNA. Translation: AAH19436.1.
    BC056608 mRNA. Translation: AAH56608.1.
    CCDSiCCDS23244.1.
    RefSeqiNP_598744.1. NM_133983.4.
    UniGeneiMm.5356.

    Genome annotation databases

    EnsembliENSMUST00000039788; ENSMUSP00000042681; ENSMUSG00000035914.
    ENSMUST00000165365; ENSMUSP00000129418; ENSMUSG00000035914.
    GeneIDi102657.
    KEGGimmu:102657.
    UCSCiuc009pxb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY190318 mRNA. Translation: AAP04007.1 .
    AK155114 mRNA. Translation: BAE33058.1 .
    BC019436 mRNA. Translation: AAH19436.1 .
    BC056608 mRNA. Translation: AAH56608.1 .
    CCDSi CCDS23244.1.
    RefSeqi NP_598744.1. NM_133983.4.
    UniGenei Mm.5356.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4I0K X-ray 2.97 A 34-247 [» ]
    ProteinModelPortali Q8VE98.
    SMRi Q8VE98. Positions 35-239.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60156N.

    PTM databases

    PhosphoSitei Q8VE98.

    Proteomic databases

    PRIDEi Q8VE98.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000039788 ; ENSMUSP00000042681 ; ENSMUSG00000035914 .
    ENSMUST00000165365 ; ENSMUSP00000129418 ; ENSMUSG00000035914 .
    GeneIDi 102657.
    KEGGi mmu:102657.
    UCSCi uc009pxb.1. mouse.

    Organism-specific databases

    CTDi 80381.
    MGIi MGI:2183926. Cd276.

    Phylogenomic databases

    eggNOGi NOG85729.
    GeneTreei ENSGT00700000104325.
    HOGENOMi HOG000293131.
    HOVERGENi HBG079786.
    InParanoidi Q8VE98.
    KOi K06746.
    OMAi ETHASVT.
    OrthoDBi EOG7FBRH9.
    PhylomeDBi Q8VE98.
    TreeFami TF331083.

    Miscellaneous databases

    NextBioi 355600.
    PROi Q8VE98.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VE98.
    Bgeei Q8VE98.
    CleanExi MM_CD276.
    Genevestigatori Q8VE98.

    Family and domain databases

    Gene3Di 2.60.40.10. 2 hits.
    InterProi IPR013162. CD80_C2-set.
    IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR003599. Ig_sub.
    IPR013106. Ig_V-set.
    [Graphical view ]
    Pfami PF08205. C2-set_2. 1 hit.
    PF07686. V-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
      Strain: C57BL/6.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Colon and Mammary tumor.
    5. "Duplication of primate and rodent B7-H3 immunoglobulin V- and C-like domains: divergent history of functional redundancy and exon loss."
      Ling V., Wu P.W., Spaulding V., Kieleczawa J., Luxenberg D., Carreno B.M., Collins M.
      Genomics 82:365-377(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOMIC DOMAIN DUPLICATION.
    6. Cited for: FUNCTION, GENE TRANSFER.
    7. Cited for: FUNCTION.
    8. Cited for: FUNCTION, INDUCTION.
    9. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
      Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
      Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-189 AND ASN-197.
      Tissue: Myoblast.
    10. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-189 AND ASN-215.

    Entry informationi

    Entry nameiCD276_MOUSE
    AccessioniPrimary (citable) accession number: Q8VE98
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2006
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Gene transfer of B7-H3 leads to complete regression of 50 per cent tumors, or significantly slows tumor growth.
    In primates, B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3