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Q8VE98

- CD276_MOUSE

UniProt

Q8VE98 - CD276_MOUSE

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Protein
CD276 antigen
Gene
Cd276, B7h3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Modulates T-cell-mediated immune responses and the development of acute and chronic transplant rejection. Plays a positive regulatory role in bone formation and has a dual role in the bone-immune interface. Induces antitumor immunity as it activates both acquired and innate immunity leading to natural killer cell and CD8 T-cell dependent killing of tumor cells.5 Publications

GO - Molecular functioni

  1. receptor binding Source: MGI
Complete GO annotation...

GO - Biological processi

  1. T cell activation Source: Ensembl
  2. negative regulation of T cell proliferation Source: MGI
  3. negative regulation of inflammatory response Source: MGI
  4. negative regulation of interferon-gamma biosynthetic process Source: MGI
  5. negative regulation of interleukin-2 biosynthetic process Source: MGI
  6. positive regulation of T cell proliferation Source: MGI
  7. positive regulation of bone mineralization Source: MGI
  8. positive regulation of interferon-gamma biosynthetic process Source: Ensembl
  9. positive regulation of interleukin-2 secretion Source: MGI
  10. positive regulation of osteoblast differentiation Source: MGI
  11. regulation of immune response Source: MGI
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
CD276 antigen
Alternative name(s):
B7 homolog 3
Short name:
B7-H3
Costimulatory molecule
CD_antigen: CD276
Gene namesi
Name:Cd276
Synonyms:B7h3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2183926. Cd276.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 248220Extracellular Reviewed prediction
Add
BLAST
Transmembranei249 – 26921Helical; Reviewed prediction
Add
BLAST
Topological domaini270 – 31647Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display a lower bone mineral density in cortical bones with femurs more susceptible to bone fracture, but do not exhibit any important skeletal abnormalities. Calvarial cells reveal normal number of osteoblast precursor cells with adequate proliferative capacity, but have impaired osteogenic differentiation. Furthermore, following cardiac transplantation, they display permanent survival under rapamycin regimen and cardiac transplants also show markedly decreased production of key cytokine and chemokine. The incidence of chronic transplant rejection is also inhibited. Mice also develop more severe airway inflammation and experimental autoimmune encephalomyelitis earlier than wild-type littermates as well as accumulate higher concentrations of autoantibodies to DNA.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed prediction
Add
BLAST
Chaini29 – 316288CD276 antigen
PRO_0000045802Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi104 – 1041N-linked (GlcNAc...)2 Publications
Disulfide bondi165 ↔ 220 By similarity
Glycosylationi189 – 1891N-linked (GlcNAc...)2 Publications
Glycosylationi197 – 1971N-linked (GlcNAc...); atypical1 Publication
Glycosylationi215 – 2151N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ8VE98.

PTM databases

PhosphoSiteiQ8VE98.

Expressioni

Tissue specificityi

Ubiquitous.1 Publication

Developmental stagei

Highly expressed in developing bones during embryogenesis and expression increases as osteoblast precursor cells differentiate into mature osteoblasts.1 Publication

Inductioni

Up-regulated in cells mediating rejection of mouse transplant.1 Publication

Gene expression databases

ArrayExpressiQ8VE98.
BgeeiQ8VE98.
CleanExiMM_CD276.
GenevestigatoriQ8VE98.

Interactioni

Subunit structurei

Interacts with TREML2 and this interaction enhances T-cell activation By similarity.

Protein-protein interaction databases

DIPiDIP-60156N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi46 – 483
Turni60 – 623
Beta strandi63 – 697
Turni70 – 723
Beta strandi75 – 806
Beta strandi83 – 897
Turni97 – 993
Helixi100 – 1023
Beta strandi107 – 1093
Helixi114 – 1163
Beta strandi120 – 1267
Beta strandi146 – 1483
Beta strandi159 – 17214
Beta strandi175 – 1795
Beta strandi188 – 1969
Beta strandi202 – 21110
Beta strandi217 – 2248
Turni225 – 2284
Beta strandi229 – 2368

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4I0KX-ray2.97A34-247[»]
ProteinModelPortaliQ8VE98.
SMRiQ8VE98. Positions 35-239.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini29 – 139111Ig-like V-type
Add
BLAST
Domaini145 – 23894Ig-like C2-type
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG85729.
GeneTreeiENSGT00700000104325.
HOGENOMiHOG000293131.
HOVERGENiHBG079786.
InParanoidiQ8VE98.
KOiK06746.
OMAiETHASVT.
OrthoDBiEOG7FBRH9.
PhylomeDBiQ8VE98.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VE98-1 [UniParc]FASTAAdd to Basket

« Hide

MLRGWGGPSV GVCVRTALGV LCLCLTGAVE VQVSEDPVVA LVDTDATLRC    50
SFSPEPGFSL AQLNLIWQLT DTKQLVHSFT EGRDQGSAYS NRTALFPDLL 100
VQGNASLRLQ RVRVTDEGSY TCFVSIQDFD SAAVSLQVAA PYSKPSMTLE 150
PNKDLRPGNM VTITCSSYQG YPEAEVFWKD GQGVPLTGNV TTSQMANERG 200
LFDVHSVLRV VLGANGTYSC LVRNPVLQQD AHGSVTITGQ PLTFPPEALW 250
VTVGLSVCLV VLLVALAFVC WRKIKQSCEE ENAGAEDQDG DGEGSKTALR 300
PLKPSENKED DGQEIA 316
Length:316
Mass (Da):34,001
Last modified:March 1, 2002 - v1
Checksum:i7BA30B1E67F55827
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY190318 mRNA. Translation: AAP04007.1.
AK155114 mRNA. Translation: BAE33058.1.
BC019436 mRNA. Translation: AAH19436.1.
BC056608 mRNA. Translation: AAH56608.1.
CCDSiCCDS23244.1.
RefSeqiNP_598744.1. NM_133983.4.
UniGeneiMm.5356.

Genome annotation databases

EnsembliENSMUST00000039788; ENSMUSP00000042681; ENSMUSG00000035914.
ENSMUST00000165365; ENSMUSP00000129418; ENSMUSG00000035914.
GeneIDi102657.
KEGGimmu:102657.
UCSCiuc009pxb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AY190318 mRNA. Translation: AAP04007.1 .
AK155114 mRNA. Translation: BAE33058.1 .
BC019436 mRNA. Translation: AAH19436.1 .
BC056608 mRNA. Translation: AAH56608.1 .
CCDSi CCDS23244.1.
RefSeqi NP_598744.1. NM_133983.4.
UniGenei Mm.5356.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4I0K X-ray 2.97 A 34-247 [» ]
ProteinModelPortali Q8VE98.
SMRi Q8VE98. Positions 35-239.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-60156N.

PTM databases

PhosphoSitei Q8VE98.

Proteomic databases

PRIDEi Q8VE98.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000039788 ; ENSMUSP00000042681 ; ENSMUSG00000035914 .
ENSMUST00000165365 ; ENSMUSP00000129418 ; ENSMUSG00000035914 .
GeneIDi 102657.
KEGGi mmu:102657.
UCSCi uc009pxb.1. mouse.

Organism-specific databases

CTDi 80381.
MGIi MGI:2183926. Cd276.

Phylogenomic databases

eggNOGi NOG85729.
GeneTreei ENSGT00700000104325.
HOGENOMi HOG000293131.
HOVERGENi HBG079786.
InParanoidi Q8VE98.
KOi K06746.
OMAi ETHASVT.
OrthoDBi EOG7FBRH9.
PhylomeDBi Q8VE98.
TreeFami TF331083.

Miscellaneous databases

NextBioi 355600.
PROi Q8VE98.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q8VE98.
Bgeei Q8VE98.
CleanExi MM_CD276.
Genevestigatori Q8VE98.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR013162. CD80_C2-set.
IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
[Graphical view ]
Pfami PF08205. C2-set_2. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    Strain: C57BL/6.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Colon and Mammary tumor.
  5. "Duplication of primate and rodent B7-H3 immunoglobulin V- and C-like domains: divergent history of functional redundancy and exon loss."
    Ling V., Wu P.W., Spaulding V., Kieleczawa J., Luxenberg D., Carreno B.M., Collins M.
    Genomics 82:365-377(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOMIC DOMAIN DUPLICATION.
  6. Cited for: FUNCTION, GENE TRANSFER.
  7. Cited for: FUNCTION.
  8. Cited for: FUNCTION, INDUCTION.
  9. "The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
    Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
    Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-189 AND ASN-197.
    Tissue: Myoblast.
  10. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-104; ASN-189 AND ASN-215.

Entry informationi

Entry nameiCD276_MOUSE
AccessioniPrimary (citable) accession number: Q8VE98
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Gene transfer of B7-H3 leads to complete regression of 50 per cent tumors, or significantly slows tumor growth.
In primates, B7-H3 locus underwent genomic duplication leading to tandemly repeated immunoglobulin-like V and C domains (VC domains). The dominantly expressed human B7-H3 isoform contains tandemly duplicated VC domains. In contrast, mouse B7-H3 transcript contains only one single VC domain form due to an exon structure corresponding to V domain-(pseudoexon C)-(pseudoexon V)-C domain. This duplication appearing in primates is suggested to be very recent supporting a model of multiple independent emergence of tandem VC repeats within human and monkey species.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi