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Protein

Serine/arginine-rich splicing factor 4

Gene

Srsf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Plays a role in alternative splice site selection during pre-mRNA splicing. Represses the splicing of MAPT/Tau exon 10 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/arginine-rich splicing factor 4
Alternative name(s):
Splicing factor, arginine/serine-rich 4
Gene namesi
Name:Srsf4
Synonyms:Sfrs4
ORF Names:MNCb-2616
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1890577. Srsf4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 489489Serine/arginine-rich splicing factor 4PRO_0000081926Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei78 – 781PhosphoserineBy similarity
Modified residuei441 – 4411PhosphoserineBy similarity
Modified residuei453 – 4531PhosphoserineBy similarity

Post-translational modificationi

Extensively phosphorylated on serine residues in the RS domain.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VE97.
MaxQBiQ8VE97.
PaxDbiQ8VE97.
PRIDEiQ8VE97.

PTM databases

iPTMnetiQ8VE97.
PhosphoSiteiQ8VE97.

Expressioni

Gene expression databases

CleanExiMM_SFRS4.

Interactioni

Subunit structurei

Found in a pre-mRNA splicing complex with SRSF4/SFRS4, SRSF5/SFRS5, SNRNP70, SNRPA1, SRRM1 and SRRM2. Interacts with PNN (By similarity).By similarity

Protein-protein interaction databases

IntActiQ8VE97. 1 interaction.
MINTiMINT-4134372.
STRINGi10090.ENSMUSP00000061474.

Structurei

3D structure databases

ProteinModelPortaliQ8VE97.
SMRiQ8VE97. Positions 3-78, 93-164.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7271RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini104 – 17774RRM 2PROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi72 – 9726Gly-rich (hinge region)Add
BLAST
Compositional biasi180 – 489310Arg/Ser-rich (RS domain)Add
BLAST

Sequence similaritiesi

Belongs to the splicing factor SR family.Curated
Contains 2 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
HOVERGENiHBG002295.
InParanoidiQ8VE97.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VE97-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRVYIGRLS YQARERDVER FFKGYGKILE VDLKNGYGFV EFDDLRDADD
60 70 80 90 100
AVYELNGKDL CGERVIVEHA RGPRRDGSYG SGRSGYGYRR SGRDKYGPPT
110 120 130 140 150
RTEYRLIVEN LSSRCSWQDL KDYMRQAGEV TYADAHKGRK NEGVIEFVSY
160 170 180 190 200
SDMKRALEKL DGTEVNGRKI RLVEDKPGSR RRRSYSRSRS HSRSRSRSRH
210 220 230 240 250
SRKSRSRSGS SKSSHSKSRS RSRSGSHSRS KSRSRSQSRS RSKKEKSRSP
260 270 280 290 300
SKDNKSRSRS RSPDKSRSKS KDHAEDKLQN NDSAGKAKSH SPSRHDSKSR
310 320 330 340 350
SRSQERRAEE ERRRSVSRAR SQEKSRSQEK SLLKSRSRSR SRSRSRSKDK
360 370 380 390 400
RKGRKRSRDE SRSRSRSKSE RSRKHSSKRD SKVSSSSSSS KKKKDTDHSR
410 420 430 440 450
SPSRSVSKER EHAKAESGQR GSRAEGESEA PNPEPRARSR STSKSKPNVP
460 470 480
AESRSRSKSA SKTRSRSKSP SRSASRSPSR SRSRSHSRS
Length:489
Mass (Da):55,979
Last modified:March 1, 2002 - v1
Checksum:i8D5FE8D1EF4624B3
GO

Sequence cautioni

The sequence BAA95070.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3411S → SKVGS (Ref. 2) Curated
Sequence conflicti389 – 3902Missing (Ref. 2) Curated
Sequence conflicti421 – 4211G → E in BAA95070 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019437 mRNA. Translation: AAH19437.1.
AB041587 mRNA. Translation: BAA95070.1. Different initiation.
UniGeneiMm.2478.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019437 mRNA. Translation: AAH19437.1.
AB041587 mRNA. Translation: BAA95070.1. Different initiation.
UniGeneiMm.2478.

3D structure databases

ProteinModelPortaliQ8VE97.
SMRiQ8VE97. Positions 3-78, 93-164.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VE97. 1 interaction.
MINTiMINT-4134372.
STRINGi10090.ENSMUSP00000061474.

PTM databases

iPTMnetiQ8VE97.
PhosphoSiteiQ8VE97.

Proteomic databases

EPDiQ8VE97.
MaxQBiQ8VE97.
PaxDbiQ8VE97.
PRIDEiQ8VE97.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1890577. Srsf4.

Phylogenomic databases

eggNOGiKOG0106. Eukaryota.
COG0724. LUCA.
HOVERGENiHBG002295.
InParanoidiQ8VE97.

Miscellaneous databases

ChiTaRSiSrsf4. mouse.
PROiQ8VE97.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SFRS4.

Family and domain databases

Gene3Di3.30.70.330. 2 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
[Graphical view]
SMARTiSM00360. RRM. 2 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  2. "Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method."
    Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 147-489.
    Strain: C57BL/6J.
    Tissue: Brain.

Entry informationi

Entry nameiSRSF4_MOUSE
AccessioniPrimary (citable) accession number: Q8VE97
Secondary accession number(s): Q9JJC3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2002
Last sequence update: March 1, 2002
Last modified: March 16, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.