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Protein

Reticulophagy regulator 1

Gene

Retreg1d

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Endoplasmic reticulum-anchored autophagy receptor that mediates ER delivery into lysosomes through sequestration into autophagosomes (PubMed:26040720). Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins (PubMed:26040720). Required for long-term survival of nociceptive and autonomic ganglion neurons (PubMed:19838196, PubMed:26040720).2 Publications

GO - Biological processi

  • negative regulation of neuron apoptotic process Source: GO_Central
  • reticulophagy Source: GO_Central
  • sensory perception of pain Source: UniProtKB

Keywordsi

Biological processAutophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Reticulophagy regulator 1
Alternative name(s):
Reticulophagy receptor 1Curated
Gene namesi
Name:Retreg1d
Synonyms:Fam134b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1913520. Fam134b.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 42CytoplasmicBy similarityAdd BLAST42
Transmembranei43 – 64HelicalSequence analysisAdd BLAST22
Topological domaini65 – 75LumenalCuratedAdd BLAST11
Transmembranei76 – 93HelicalSequence analysisAdd BLAST18
Topological domaini94 – 98CytoplasmicCurated5
Transmembranei99 – 116HelicalSequence analysisAdd BLAST18
Topological domaini117 – 191LumenalCuratedAdd BLAST75
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 480CytoplasmicBy similarityAdd BLAST268

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to an expanded endoplasmic reticulum.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002884671 – 480Reticulophagy regulator 1Add BLAST480

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei340PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VE91.
PaxDbiQ8VE91.
PeptideAtlasiQ8VE91.
PRIDEiQ8VE91.

PTM databases

iPTMnetiQ8VE91.
PhosphoSitePlusiQ8VE91.

Expressioni

Tissue specificityi

Predominantly expressed in sensory and autonomic ganglia has demonstrated by in situ hybridizations of embryonic day 14.5 mouse embryo sections. Detected specifically in organelle-like intracellular structures of the small and large neurons of dorsal root ganglia (DRG) and in Neuro-2a cells, a tumor cell line that is related to autonomic ganglion neurons (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000022270.
GenevisibleiQ8VE91. MM.

Interactioni

Subunit structurei

Interacts with ATG8 family modifier proteins MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2.By similarity

Protein-protein interaction databases

DIPiDIP-61579N.
IntActiQ8VE91. 1 interactor.
STRINGi10090.ENSMUSP00000022881.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni67 – 216Reticulon homology domain1 PublicationAdd BLAST150

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi436 – 441LIR motifBy similarity6

Domaini

The LIR motif interacts with ATG8 family proteins and is necessary to target the ER fragments to autophagosomes for lysosomal degradation.1 Publication
The reticulon homology domain provides capacity to bend the membrane and promotes ER scission (PubMed:26040720). This domain does not show relevant similarities with reticulon domains, preventing any domain predictions within the protein sequence.Curated1 Publication

Sequence similaritiesi

Belongs to the RETREG family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFP1. Eukaryota.
ENOG410XPQB. LUCA.
GeneTreeiENSGT00530000063240.
HOGENOMiHOG000050246.
HOVERGENiHBG071073.
InParanoidiQ8VE91.
OMAiLSESWEV.
OrthoDBiEOG091G08S3.
TreeFamiTF329111.

Family and domain databases

InterProiView protein in InterPro
IPR033358. RETREG1.
PANTHERiPTHR28659:SF3. PTHR28659:SF3. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 4 (identifier: Q8VE91-4) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPAPEEHA TQGCPATEEQ PPRPGVPGEE AGPEGAGPQV EEAAGRVAAA
60 70 80 90 100
LTWLLGEPVL WLGWRADELL SWKRPLRSLL TFLGANLLFW FLALTPWRVY
110 120 130 140 150
HLISVMILGR VIMQIIKEMV LSRTRGAQLW RSLTESWEVI NSKPDERARL
160 170 180 190 200
SQCIAESWMN FSMFLQEMSL FKQQSPGKFC LLVCSVCTFF TILGSYIPGV
210 220 230 240 250
ILSYLLLLFA FLCPLFKCND IGQKIYSKVK SILLKLDFGI GEYINQKKRE
260 270 280 290 300
RSEADKEKSH KDDSELDFSA LCPKISLTVA AKELSVSDTD VSEVSWTDNG
310 320 330 340 350
TFNLSEGYTP QTDTSDDLDR PSEEVFSRDL SDFPSLENGT GTNDEDELSL
360 370 380 390 400
GLPTELKRKK QQLDSAHRPS KERQSAAGLS LPLKSDQALH LMSNLAGDVI
410 420 430 440 450
TAAMTAAIKD QLEGARQALT QVAPTAGEDT DTEEGDDFEL LDQAELDQIE
460 470 480
SELGLTQDQG AEAQQSKKSS GFLSNLLGGH
Note: Predicted by similarity to human, no experimental confirmation available.
Length:480
Mass (Da):52,967
Last modified:September 16, 2015 - v2
Checksum:i6181AF418CBCC29A
GO
Isoform 1 (identifier: Q8VE91-1) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-134: MASPAPEEHA...RGAQLWRSLT → MLGDWAALLPSCLSRCWS

Show »
Length:364
Mass (Da):40,142
Checksum:iF9CABAA6944E3510
GO
Isoform 2 (identifier: Q8VE91-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: MASPAPEEHA...GAQLWRSLTE → MPAGGGCGPGR

Show »
Length:356
Mass (Da):38,963
Checksum:i94B899C82D774FA9
GO
Isoform 3 (identifier: Q8VE91-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-135: MASPAPEEHA...GAQLWRSLTE → M

Show »
Length:346
Mass (Da):38,153
Checksum:iA49678F437BB1459
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0578361 – 135MASPA…RSLTE → MPAGGGCGPGR in isoform 2. Add BLAST135
Alternative sequenceiVSP_0578371 – 135MASPA…RSLTE → M in isoform 3. Add BLAST135
Alternative sequenceiVSP_0578381 – 134MASPA…WRSLT → MLGDWAALLPSCLSRCWS in isoform 1. Add BLAST134

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007506 mRNA. Translation: BAB25077.1.
AK146972 mRNA. Translation: BAE27577.1.
AK015887 mRNA. Translation: BAB30019.1.
AK167327 mRNA. Translation: BAE39429.1.
AC131178 Genomic DNA. No translation available.
AC162303 Genomic DNA. No translation available.
BC019494 mRNA. Translation: AAH19494.1.
BC054415 mRNA. Translation: AAH54415.1.
CCDSiCCDS37050.1. [Q8VE91-4]
CCDS37051.1. [Q8VE91-2]
RefSeqiNP_001030023.1. NM_001034851.2. [Q8VE91-4]
NP_001264244.1. NM_001277315.1. [Q8VE91-2]
NP_001264245.1. NM_001277316.1. [Q8VE91-1]
NP_001264246.1. NM_001277317.1. [Q8VE91-3]
NP_079735.2. NM_025459.3. [Q8VE91-2]
XP_006520197.1. XM_006520134.3. [Q8VE91-2]
XP_017172209.1. XM_017316720.1. [Q8VE91-2]
UniGeneiMm.25311.

Genome annotation databases

EnsembliENSMUST00000022881; ENSMUSP00000022881; ENSMUSG00000022270. [Q8VE91-4]
ENSMUST00000110438; ENSMUSP00000106068; ENSMUSG00000022270. [Q8VE91-2]
GeneIDi66270.
KEGGimmu:66270.
UCSCiuc007vjf.2. mouse.
uc007vjg.2. mouse. [Q8VE91-4]
uc007vji.2. mouse. [Q8VE91-2]
uc033gth.1. mouse. [Q8VE91-3]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiRETR1_MOUSE
AccessioniPrimary (citable) accession number: Q8VE91
Secondary accession number(s): E9QPM1
, Q7TMY5, Q9CUJ4, Q9D8Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: September 16, 2015
Last modified: October 25, 2017
This is version 108 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families