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Q8VE73 (CUL7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cullin-7

Short name=CUL-7
Alternative name(s):
p185
p193
Gene names
Name:Cul7
Synonyms:Kiaa0076
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1689 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of a probable SCF-like E3 ubiquitin-protein ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. In complex with FBXW8, mediates ubiquitination and consequent degradation of GORASP1, acting as a component of the ubiquitin ligase pathway that regulates Golgi morphogenesis and dendrite patterning in brain By similarity. Probably plays a role in the degradation of proteins involved in endothelial proliferation and/or differentiation. Ref.5

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone. Interacts with CUL9. Interacts with FBXW8; interaction is mutually exclusive of binding to CUL9 or TP53. Interacts with TP53; the interaction preferentially involves tetrameric and dimeric TP53. The CUL7-CUL9 heterodimer seems to interact specifically with TP53 By similarity. Interacts with OBSL1 By similarity. Interacts with CUL1; the interaction seems to be mediated by FBXW8. Ref.6

Subcellular location

Cytoplasm By similarity. Cytoplasmperinuclear region By similarity. Golgi apparatus By similarity. Note: Colocalizes with FBXW8 at the Golgi apparatus in neurons; localization to Golgi is mediated by OBSL1 By similarity.

Disruption phenotype

Mice are neonatal lethal and show vascular defects in both the embryo and the placenta. Ref.5

Sequence similarities

Belongs to the cullin family.

Contains 1 DOC domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VE73-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VE73-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1472-1472: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 16891689Cullin-7
PRO_0000119803

Regions

Domain793 – 972180DOC
Region349 – 449101Interaction with TP53 By similarity
Compositional bias1308 – 137063Glu-rich

Amino acid modifications

Cross-link1567Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in NEDD8) Potential

Natural variations

Alternative sequence14721Missing in isoform 2.
VSP_038331

Experimental info

Sequence conflict3951G → V in BAE27723. Ref.1
Sequence conflict5181D → E in BAD32160. Ref.4
Sequence conflict777 – 7793IQM → DAW in AAH19645. Ref.3
Sequence conflict855 – 92268Missing in AAH59865. Ref.3
Sequence conflict8881G → R in BAE27723. Ref.1
Sequence conflict9591Q → E in AAH59865. Ref.3
Sequence conflict14821S → L in BAB25811. Ref.1
Sequence conflict14951P → L in BAB25811. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 9, 2007. Version 2.
Checksum: 6ED4641B577E53CD

FASTA1,689192,292
        10         20         30         40         50         60 
MVGELRYREF RVPLGPGLHA YPDELIRQRV GHNGHPEYQI RWLILRRGDD GDRDSTVDCK 

        70         80         90        100        110        120 
AEHILLWMSD DEIYANCHKM LGENGQVIAP SRESTEAGAL DKSVLGEMET DVKSLIQRAL 

       130        140        150        160        170        180 
RQLEECVGTV PPAPLLHTVH VLSAYASIEP LTGIFKDRRV VNLLMHMLSS PDYQIRWSAG 

       190        200        210        220        230        240 
RMIQALSSHD AGTRTQILLS LSQQEAIEKH LDFDSRCALL ALFAQATLTE HPMSFEGVQL 

       250        260        270        280        290        300 
PQVPGRLLFS LVKRYLHVTF LLDRLNGDAG DQGAQNNFIP EELNVGRGRL ELEFSMAMGT 

       310        320        330        340        350        360 
LISELVQAMR WDGASSRPES SSSSTFQPRP AQFRPYTQRF RRSRRFRPRA SFASFNTYAL 

       370        380        390        400        410        420 
YVRDTLRPGM RVRMLENYEE IAAGDEGQFR QSNDGVPPAQ VLWDSTGHTY WVHWHMLEIL 

       430        440        450        460        470        480 
GFEEDIEDVI DIEELQELGA NGALSIVPPS QRWKPITQLF AEPYVVPEEE DREESENLTQ 

       490        500        510        520        530        540 
AEWWELLFFI RQLSEAERLH IVDLLQDHLE EERVLDYDML PELTVPVDLA QDLLLSLPQQ 

       550        560        570        580        590        600 
LEDSALRDLF SCSVYRKYGP EVLVGHLSYP FVPGAQPNLF GANEESEAKD PPLQSASPAL 

       610        620        630        640        650        660 
QRLVESLGPE GEVLVELEQA LGSEAPQETE VKSCLLQLQE QPQPFLALMR SLDTSASNKT 

       670        680        690        700        710        720 
LHLTVLRILM QLVNFPEALL LPWHEAMDAC VTCLRSPNTD REVLQELIFF LHRLTTTSRD 

       730        740        750        760        770        780 
YAVILNQLGA RDAISKVLEK HRGKLELAQE LRDMVSKCEK HAHLYRKLTT NILGGCIQMV 

       790        800        810        820        830        840 
LGQIEDHRRT HRPIQIPFFD VFLRYLCQGS SEEMKKNRYW EKVEVSSNPQ RASRLTDRNP 

       850        860        870        880        890        900 
KTYWESSGRA GSHFITLHMR PGVIIRQLTL LVAGEDSSYM PAWVVVCGGN SIKSVNKELN 

       910        920        930        940        950        960 
TVNVMPSASR VTLLENLTRF WPIIQIRIKR CQQGGINTRI RGLEVLGPKP TFWPVFREQL 

       970        980        990       1000       1010       1020 
CRHTRLFYMV RAQAWSQDIA EDRRSLLHLS SRLNGALRHE QNFAERFLPD MEAAQALSKT 

      1030       1040       1050       1060       1070       1080 
CWEALVSPLV QNITSPDEDS TSSLGWLLDQ YLGCREAAYN PQSRAAAFSS RVRRLTHLLV 

      1090       1100       1110       1120       1130       1140 
HVEPREAAPP VVAIPRSKGR NRIHDWSYLI TRGLPSSIMK NLTRCWRSVV EEQMNKFLTA 

      1150       1160       1170       1180       1190       1200 
SWKDDDFVPR YCERYYVLQK SSSELFGPRA AFLLAMRNGC ADAVLRLPFL RAAHVSEQFA 

      1210       1220       1230       1240       1250       1260 
RHIDQRIQGS RMGGARGMEM LAQLQRCLES VLIFSPLEIA TTFEHYYQHY MADRLLSVGS 

      1270       1280       1290       1300       1310       1320 
SWLEGAVLEQ IGPCFPSRLP QQMLQSLNVS EELQRQFHVY QLQQLDQELL KLEDTEKKIQ 

      1330       1340       1350       1360       1370       1380 
VAHEDSGRED KSKKEEAIGE AAAVAMAEEE DQGKKEEGEE EGEGEDEEEE RYYKGTMPEV 

      1390       1400       1410       1420       1430       1440 
CVLVVTPRFW PVASVCQMLN PATCLPAYLR GTINHYTNFY SKSQSRSSLE KEPQRRLQWT 

      1450       1460       1470       1480       1490       1500 
WQGRAEVQFG GQILHVSTVQ MWLLLHLNNQ KEVSVESLQA ISELPPDVLH RAIGPLTSSR 

      1510       1520       1530       1540       1550       1560 
GPLDLQEQKN VPGGVLKIRD DSEEPRPRRG NVWLIPPQTY LQAEAEEGRN MEKRRNLLNC 

      1570       1580       1590       1600       1610       1620 
LVVRILKAHG DEGLHVDRLV YLVLEAWEKG PCPARGLVSS LGRGATCRSS DVLSCILHLL 

      1630       1640       1650       1660       1670       1680 
VKGTLRRHDD RPQVLYYAVP VTVMEPHMES LNPGSAGPNP PLTFHTLQIR SRGVPYASCT 


DNHTFSTFR 

« Hide

Isoform 2 [UniParc].

Checksum: 8AD13BBC66EAA8A5
Show »

FASTA1,688192,163

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C57BL/6J.
Tissue: Stomach.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 299-1689 (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 777-1689 (ISOFORM 1).
Strain: C57BL/6 and Czech II.
Tissue: Brain, Jaw, Limb and Mammary gland.
[4]"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 182-1689 (ISOFORM 1).
Tissue: Fetal brain.
[5]"Targeted disruption of p185/Cul7 gene results in abnormal vascular morphogenesis."
Arai T., Kasper J.S., Skaar J.R., Ali S.H., Takahashi C., DeCaprio J.A.
Proc. Natl. Acad. Sci. U.S.A. 100:9855-9860(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[6]"Fbxw8 is essential for Cul1-Cul7 complex formation and for placental development."
Tsunematsu R., Nishiyama M., Kotoshiba S., Saiga T., Kamura T., Nakayama K.I.
Mol. Cell. Biol. 26:6157-6169(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CUL1 AND FBXW8.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK008655 mRNA. Translation: BAB25811.1.
AK147156 mRNA. Translation: BAE27723.1.
CT030702 Genomic DNA. No translation available.
BC019645 mRNA. Translation: AAH19645.1.
BC026946 mRNA. Translation: AAH26946.2.
BC059865 mRNA. Translation: AAH59865.1.
BC100544 mRNA. Translation: AAI00545.1.
AK172882 mRNA. Translation: BAD32160.1.
CCDSCCDS28833.1. [Q8VE73-1]
RefSeqNP_079887.3. NM_025611.5. [Q8VE73-1]
UniGeneMm.329078.

3D structure databases

ProteinModelPortalQ8VE73.
SMRQ8VE73. Positions 349-424.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid211530. 6 interactions.

PTM databases

PhosphoSiteQ8VE73.

Proteomic databases

MaxQBQ8VE73.
PaxDbQ8VE73.
PRIDEQ8VE73.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000043464; ENSMUSP00000049128; ENSMUSG00000038545. [Q8VE73-1]
GeneID66515.
KEGGmmu:66515.
UCSCuc008ctp.1. mouse. [Q8VE73-1]

Organism-specific databases

CTD9820.
MGIMGI:1913765. Cul7.
RougeSearch...

Phylogenomic databases

eggNOGNOG48148.
GeneTreeENSGT00700000104406.
HOVERGENHBG103792.
InParanoidQ8VE73.
KOK10613.
OMATFEHYYQ.
OrthoDBEOG70S74H.
PhylomeDBQ8VE73.
TreeFamTF101154.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressQ8VE73.
BgeeQ8VE73.
CleanExMM_CUL7.
GenevestigatorQ8VE73.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.25.10.10. 3 hits.
2.30.30.30. 1 hit.
2.60.120.260. 1 hit.
InterProIPR004939. APC_su10/DOC_dom.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR021097. CPH_domain.
IPR016157. Cullin_CS.
IPR016158. Cullin_homology.
IPR001373. Cullin_N.
IPR008979. Galactose-bd-like.
IPR014722. Rib_L2_dom2.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF03256. APC10. 1 hit.
PF11515. Cul7. 1 hit.
PF00888. Cullin. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 2 hits.
SSF49785. SSF49785. 1 hit.
SSF75632. SSF75632. 2 hits.
PROSITEPS01256. CULLIN_1. 1 hit.
PS50069. CULLIN_2. 1 hit.
PS51284. DOC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCUL7. mouse.
NextBio321916.
PROQ8VE73.
SOURCESearch...

Entry information

Entry nameCUL7_MOUSE
AccessionPrimary (citable) accession number: Q8VE73
Secondary accession number(s): Q3UHY3 expand/collapse secondary AC list , Q497I2, Q6A0D6, Q6PB63, Q8R3W4, Q9CVD5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: January 9, 2007
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot