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Q8VE65 (TAF12_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Transcription initiation factor TFIID subunit 12
Alternative name(s):
Transcription initiation factor TFIID 20 kDa subunits
Short name=TAFII-20
Short name=TAFII20
Gene names
Name:Taf12
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length161 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription By similarity.

Subunit structure

TFIID and PCAF are composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs). TBP is not part of TFTC. Interacts directly with TBP; additional interactions between TAFII20 and TAFII28 or TAFII30 were detected. Component of the PCAF complex, at least composed of TADA2L/ADA2, TADA3L/ADA3, TAF5L, SUPT3H, TAF6L, TAF9, TAF10, TAF12 and TRRAP. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9. Interacts with ATF7 (via its transactivation domain); the interaction promotes the transactivation of ATF7 and is prevented by sumoylation of ATF7 By similarity.

Subcellular location

Nucleus By similarity.

Sequence similarities

Belongs to the TAF12 family.

Contains 1 histone-fold domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 161161Transcription initiation factor TFIID subunit 12
PRO_0000118908

Amino acid modifications

Modified residue431Phosphothreonine By similarity
Modified residue511Phosphoserine Ref.3 Ref.4
Modified residue591Phosphothreonine By similarity

Experimental info

Sequence conflict771D → G in BAB28669. Ref.1
Sequence conflict1261M → I in BAB28669. Ref.1
Sequence conflict1571K → N in BAB25276. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8VE65 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: B3C11D70B6E94E35

FASTA16117,875
        10         20         30         40         50         60 
MNQFGPSALI NLSSFSSVKP EPASTPPQGS MANSTTVGKI AGTPGTGGRL SPENNQVLTK 

        70         80         90        100        110        120 
KKLQDLVREV DPNEQLDEDV EEMLLQIADD FIESVVTAAC QLARHRKSST LEVKDVQLHL 

       130        140        150        160 
ERQWNMWIPG FGSEEIRPYK KACTTEAHKQ RMALIRKTTK K 

« Hide

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo, Pancreas and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[4]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK007811 mRNA. Translation: BAB25276.1.
AK013133 mRNA. Translation: BAB28669.1.
AK132927 mRNA. Translation: BAE21426.1.
BC019668 mRNA. Translation: AAH19668.1.
CCDSCCDS18721.1.
RefSeqNP_079855.2. NM_025579.3.
XP_006539172.1. XM_006539109.1.
XP_006539173.1. XM_006539110.1.
UniGeneMm.331926.

3D structure databases

ProteinModelPortalQ8VE65.
SMRQ8VE65. Positions 55-128.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-29178N.
IntActQ8VE65. 3 interactions.
MINTMINT-4136826.

PTM databases

PhosphoSiteQ8VE65.

Proteomic databases

PaxDbQ8VE65.
PRIDEQ8VE65.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030731; ENSMUSP00000030731; ENSMUSG00000028899.
GeneID66464.
KEGGmmu:66464.
UCSCuc008vax.3. mouse.

Organism-specific databases

CTD6883.
MGIMGI:1913714. Taf12.

Phylogenomic databases

eggNOGCOG5624.
GeneTreeENSGT00390000002144.
HOVERGENHBG055174.
InParanoidQ8VE65.
KOK03126.
OrthoDBEOG769ZMW.
PhylomeDBQ8VE65.
TreeFamTF323652.

Gene expression databases

ArrayExpressQ8VE65.
BgeeQ8VE65.
GenevestigatorQ8VE65.

Family and domain databases

Gene3D1.10.20.10. 1 hit.
InterProIPR009072. Histone-fold.
IPR003228. TFIID_sub.
[Graphical view]
PfamPF03847. TFIID_20kDa. 1 hit.
[Graphical view]
ProDomPD012998. PD012998. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMSSF47113. SSF47113. 1 hit.
ProtoNetSearch...

Other

NextBio321768.
PROQ8VE65.
SOURCESearch...

Entry information

Entry nameTAF12_MOUSE
AccessionPrimary (citable) accession number: Q8VE65
Secondary accession number(s): Q3V0S7, Q9CZ11, Q9D8Q0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot