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Protein

Ubiquitin-like modifier-activating enzyme 5

Gene

Uba5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

E1-like enzyme which activates UFM1 and SUMO2.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811ATP; via amide nitrogenBy similarity
Binding sitei102 – 1021ATPBy similarity
Binding sitei125 – 1251ATPBy similarity
Binding sitei148 – 1481ATPBy similarity
Binding sitei182 – 1821ATPBy similarity
Metal bindingi224 – 2241ZincBy similarity
Metal bindingi227 – 2271ZincBy similarity
Active sitei248 – 2481Glycyl thioester intermediateBy similarity
Metal bindingi301 – 3011ZincBy similarity
Metal bindingi306 – 3061ZincBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like modifier-activating enzyme 5
Short name:
Ubiquitin-activating enzyme 5
Alternative name(s):
UFM1-activating enzyme
Ubiquitin-activating enzyme E1 domain-containing protein 1
Gene namesi
Name:Uba5
Synonyms:Ube1dc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1913913. Uba5.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Death at embryonic day 12.5.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 403403Ubiquitin-like modifier-activating enzyme 5PRO_0000194971Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei357 – 3571PhosphoserineBy similarity
Modified residuei392 – 3921PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VE47.
MaxQBiQ8VE47.
PaxDbiQ8VE47.
PeptideAtlasiQ8VE47.
PRIDEiQ8VE47.

PTM databases

iPTMnetiQ8VE47.
PhosphoSiteiQ8VE47.
SwissPalmiQ8VE47.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032557.
CleanExiMM_UBA5.
ExpressionAtlasiQ8VE47. baseline and differential.
GenevisibleiQ8VE47. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8VE47. 3 interactions.
MINTiMINT-4119979.
STRINGi10090.ENSMUSP00000035166.

Structurei

3D structure databases

ProteinModelPortaliQ8VE47.
SMRiQ8VE47. Positions 67-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-activating E1 family. UBA5 subfamily.Sequence analysis

Phylogenomic databases

eggNOGiKOG2336. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00810000125467.
HOGENOMiHOG000256352.
HOVERGENiHBG056496.
InParanoidiQ8VE47.
KOiK12164.
OMAiDVQIETH.
OrthoDBiEOG091G0BHP.
PhylomeDBiQ8VE47.
TreeFamiTF314168.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR029752. D-isomer_DH_CS1.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VE47-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADSVERLRQ RVEELEQELA RERTRRSGGD GHCGRTRIQE MSDEVLDSNP
60 70 80 90 100
YSRLMALKRM GIVSDYKKIR TYAVAIVGVG GVGSVTAEML TRCGIGKLLL
110 120 130 140 150
FDYDKVELAN MNRLFFQPYQ AGLSKVHAAE HTLRNINPDV LFEVHNYNIT
160 170 180 190 200
TVEHFEHFMN RISNGGLEEG QPVDLVLSCV DNFEARMAIN TACNELGQTW
210 220 230 240 250
MESGVSENAV SGHIQLMIPG ESACFACAPP LVVASNIDEK TLKREGVCAA
260 270 280 290 300
SLPTTMGVVA GILVQNVLKF LLKFGTVSFY LGYNAMQDFF PTMFMKPNPQ
310 320 330 340 350
CDDKNCRKQQ EEYKKRAAAL PTQEAEPQEE AEVVHEDNEW GIELVSEVSE
360 370 380 390 400
EELKNSSGPV PTLPEGITVA YTVPKKTEDS ASEVTVEDSG ESLEDLMARM

KNM
Length:403
Mass (Da):44,790
Last modified:March 2, 2010 - v2
Checksum:i6C022AF9E0B7C83B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 112QR → HG in BAB30933 (PubMed:16141072).Curated
Sequence conflicti30 – 301D → G in BAE30888 (PubMed:16141072).Curated
Sequence conflicti153 – 1531E → G in BAB30933 (PubMed:16141072).Curated
Sequence conflicti156 – 1561E → G in BAB30933 (PubMed:16141072).Curated
Sequence conflicti156 – 1561E → K in BAE30720 (PubMed:16141072).Curated
Sequence conflicti156 – 1561E → K in BAE30888 (PubMed:16141072).Curated
Sequence conflicti164 – 1641N → K in BAB30933 (PubMed:16141072).Curated
Sequence conflicti224 – 2241C → L in BAB30933 (PubMed:16141072).Curated
Sequence conflicti300 – 3001Q → H in AAH19764 (PubMed:15489334).Curated
Sequence conflicti402 – 4021N → T in AAH19764 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017787 mRNA. Translation: BAB30933.1.
AK151823 mRNA. Translation: BAE30720.1.
AK152029 mRNA. Translation: BAE30888.1.
AK168907 mRNA. Translation: BAE40721.1.
CH466560 Genomic DNA. Translation: EDL21084.1.
BC019764 mRNA. Translation: AAH19764.1.
CCDSiCCDS23457.1.
RefSeqiNP_079968.2. NM_025692.3.
UniGeneiMm.224935.
Mm.479441.

Genome annotation databases

EnsembliENSMUST00000035166; ENSMUSP00000035166; ENSMUSG00000032557.
GeneIDi66663.
KEGGimmu:66663.
UCSCiuc009rhf.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017787 mRNA. Translation: BAB30933.1.
AK151823 mRNA. Translation: BAE30720.1.
AK152029 mRNA. Translation: BAE30888.1.
AK168907 mRNA. Translation: BAE40721.1.
CH466560 Genomic DNA. Translation: EDL21084.1.
BC019764 mRNA. Translation: AAH19764.1.
CCDSiCCDS23457.1.
RefSeqiNP_079968.2. NM_025692.3.
UniGeneiMm.224935.
Mm.479441.

3D structure databases

ProteinModelPortaliQ8VE47.
SMRiQ8VE47. Positions 67-320.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VE47. 3 interactions.
MINTiMINT-4119979.
STRINGi10090.ENSMUSP00000035166.

PTM databases

iPTMnetiQ8VE47.
PhosphoSiteiQ8VE47.
SwissPalmiQ8VE47.

Proteomic databases

EPDiQ8VE47.
MaxQBiQ8VE47.
PaxDbiQ8VE47.
PeptideAtlasiQ8VE47.
PRIDEiQ8VE47.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035166; ENSMUSP00000035166; ENSMUSG00000032557.
GeneIDi66663.
KEGGimmu:66663.
UCSCiuc009rhf.2. mouse.

Organism-specific databases

CTDi79876.
MGIiMGI:1913913. Uba5.

Phylogenomic databases

eggNOGiKOG2336. Eukaryota.
COG0476. LUCA.
GeneTreeiENSGT00810000125467.
HOGENOMiHOG000256352.
HOVERGENiHBG056496.
InParanoidiQ8VE47.
KOiK12164.
OMAiDVQIETH.
OrthoDBiEOG091G0BHP.
PhylomeDBiQ8VE47.
TreeFamiTF314168.

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiQ8VE47.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032557.
CleanExiMM_UBA5.
ExpressionAtlasiQ8VE47. baseline and differential.
GenevisibleiQ8VE47. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR029752. D-isomer_DH_CS1.
IPR016040. NAD(P)-bd_dom.
IPR000594. ThiF_NAD_FAD-bd.
[Graphical view]
PfamiPF00899. ThiF. 1 hit.
[Graphical view]
SUPFAMiSSF69572. SSF69572. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBA5_MOUSE
AccessioniPrimary (citable) accession number: Q8VE47
Secondary accession number(s): Q3TG27
, Q3U8X9, Q3U9E7, Q9CYD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 2, 2010
Last modified: September 7, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.