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Protein

Protein naked cuticle homolog 2

Gene

Nkd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Required for processing of TGFA and for targeting of TGFA to the basolateral membrane of polarized epithelial cells (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi140 – 15112PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Exocytosis, Transport, Wnt signaling pathway

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein naked cuticle homolog 2
Short name:
Naked-2
Short name:
mNkd2
Gene namesi
Name:Nkd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1919543. Nkd2.

Subcellular locationi

  • Cell membrane By similarity
  • Cytoplasm By similarity
  • Cytoplasmic vesicle By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 461460Protein naked cuticle homolog 2PRO_0000301994Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity

Post-translational modificationi

Ubiquitinated, leading to rapid proteasomal degradation. Interaction with TGFA interferes with RNF25 binding and protects against ubiquitination mediated by RNF25 (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Myristate, Ubl conjugation

Proteomic databases

MaxQBiQ8VE28.
PaxDbiQ8VE28.
PRIDEiQ8VE28.

PTM databases

iPTMnetiQ8VE28.
PhosphoSiteiQ8VE28.

Expressioni

Tissue specificityi

Expressed in the cecum, colon, esophagus, ileum, jejunum, skin and stomach.3 Publications

Developmental stagei

Expressed in the forelimb buds, the branchial arches, the caudal presomitic mesoderm (PSM) and at the anterior and posterior of each somite boundary at 9.5 days postcoitum (dpc). Also expressed in the tailbud.4 Publications

Gene expression databases

BgeeiENSMUSG00000021567.
CleanExiMM_NKD2.
GenevisibleiQ8VE28. MM.

Interactioni

Subunit structurei

Interacts with RNF25, TGFA (via cytoplasmic domain), and PPP2R3A (By similarity). Interacts with DVL1, DVL2 and DVL3.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215287. 5 interactions.
IntActiQ8VE28. 5 interactions.
STRINGi10090.ENSMUSP00000022051.

Structurei

3D structure databases

ProteinModelPortaliQ8VE28.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini127 – 16236EF-handPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni121 – 18666Interaction with DVL1, DVL2 and DVL3Add
BLAST
Regioni307 – 39690Interaction with TGFABy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi441 – 45919His-richAdd
BLAST

Domaini

The N-terminal domain comprising the first 224 amino acid residues is mostly unstructured.By similarity

Sequence similaritiesi

Belongs to the NKD family.Curated
Contains 1 EF-hand domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IFZW. Eukaryota.
ENOG41114G9. LUCA.
GeneTreeiENSGT00440000033589.
HOGENOMiHOG000005649.
HOVERGENiHBG099718.
InParanoidiQ8VE28.
KOiK03213.
OMAiHKRYRQK.
OrthoDBiEOG091G0HUV.
PhylomeDBiQ8VE28.
TreeFamiTF328786.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VE28-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGKFQSKHAA AACKRRESPE GDSFVVPAYG SGRRGAEETD RRAGSGVEHR
60 70 80 90 100
SRDKQELLNG DPKEGPFWDD KGSLEVVLPP EKSEGHEGQG QLFSTDDGEK
110 120 130 140 150
AASREGPLRL SKKHLNIDAL QCDVSVEEDN RQEWTFTLYD FDNSGKVTRE
160 170 180 190 200
DMSSLMHTIY EVVDASVNHS SGSSKTLRVK LTVSPEPSSK KECPLTGQDR
210 220 230 240 250
EPTRGRTEIE LTDEPRVADR RLSAYSRKPN ADPQPCSVRV PYCVDENTER
260 270 280 290 300
RNHYLDLAGI ENYTSKFGPG SPPEQARQEH HGRATHIPSR SRSQESDAHA
310 320 330 340 350
IHHRRSQVLA EHVIPANEPA TRALAAQPRI KGQEKQFLRS PKGPGKPLGT
360 370 380 390 400
PGSGKPGKAL SYCLQAVPLP QSAQDGHHLP QPPPQPPPQP YGHKRYRQKA
410 420 430 440 450
REGHSPLKGH GQPTMVEHEV VRDLPPMLGP EGYVMPVVQR HEHHHHHEHH
460
HHHHHHHFHP S
Length:461
Mass (Da):51,501
Last modified:September 11, 2007 - v2
Checksum:i7C3B550024E87D27
GO
Isoform 2 (identifier: Q8VE28-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     9-20: Missing.

Note: No experimental confirmation available.
Show »
Length:449
Mass (Da):50,231
Checksum:iFC6ADFBE8836D671
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti77 – 771V → A in BAE26264 (PubMed:16141072).Curated
Sequence conflicti77 – 771V → A in AAH19952 (PubMed:15489334).Curated
Sequence conflicti217 – 2171V → A in AAH19952 (PubMed:15489334).Curated
Sequence conflicti317 – 3171N → S in BAB25908 (PubMed:16141072).Curated
Sequence conflicti330 – 3301I → L in BAE26264 (PubMed:16141072).Curated
Sequence conflicti330 – 3301I → L in BAE34467 (PubMed:16141072).Curated
Sequence conflicti330 – 3301I → L in AAH19952 (PubMed:15489334).Curated
Sequence conflicti372 – 3721S → G in BAE26264 (PubMed:16141072).Curated
Sequence conflicti372 – 3721S → G in BAE34467 (PubMed:16141072).Curated
Sequence conflicti372 – 3721S → G in AAH19952 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei9 – 2012Missing in isoform 2. 1 PublicationVSP_027902Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358136 mRNA. Translation: AAK57485.1.
AK008814 mRNA. Translation: BAB25908.1.
AK081904 mRNA. Translation: BAC38367.1.
AK145155 mRNA. Translation: BAE26264.1.
AK146667 mRNA. Translation: BAE27345.1.
AK158350 mRNA. Translation: BAE34467.1.
BC019952 mRNA. Translation: AAH19952.1.
CCDSiCCDS26636.1. [Q8VE28-1]
RefSeqiNP_082462.3. NM_028186.4. [Q8VE28-1]
XP_006517459.1. XM_006517396.2. [Q8VE28-2]
UniGeneiMm.45506.

Genome annotation databases

EnsembliENSMUST00000022051; ENSMUSP00000022051; ENSMUSG00000021567. [Q8VE28-1]
ENSMUST00000118096; ENSMUSP00000113794; ENSMUSG00000021567. [Q8VE28-2]
GeneIDi72293.
KEGGimmu:72293.
UCSCiuc007ref.2. mouse. [Q8VE28-1]
uc007reg.2. mouse. [Q8VE28-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF358136 mRNA. Translation: AAK57485.1.
AK008814 mRNA. Translation: BAB25908.1.
AK081904 mRNA. Translation: BAC38367.1.
AK145155 mRNA. Translation: BAE26264.1.
AK146667 mRNA. Translation: BAE27345.1.
AK158350 mRNA. Translation: BAE34467.1.
BC019952 mRNA. Translation: AAH19952.1.
CCDSiCCDS26636.1. [Q8VE28-1]
RefSeqiNP_082462.3. NM_028186.4. [Q8VE28-1]
XP_006517459.1. XM_006517396.2. [Q8VE28-2]
UniGeneiMm.45506.

3D structure databases

ProteinModelPortaliQ8VE28.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215287. 5 interactions.
IntActiQ8VE28. 5 interactions.
STRINGi10090.ENSMUSP00000022051.

PTM databases

iPTMnetiQ8VE28.
PhosphoSiteiQ8VE28.

Proteomic databases

MaxQBiQ8VE28.
PaxDbiQ8VE28.
PRIDEiQ8VE28.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022051; ENSMUSP00000022051; ENSMUSG00000021567. [Q8VE28-1]
ENSMUST00000118096; ENSMUSP00000113794; ENSMUSG00000021567. [Q8VE28-2]
GeneIDi72293.
KEGGimmu:72293.
UCSCiuc007ref.2. mouse. [Q8VE28-1]
uc007reg.2. mouse. [Q8VE28-2]

Organism-specific databases

CTDi85409.
MGIiMGI:1919543. Nkd2.

Phylogenomic databases

eggNOGiENOG410IFZW. Eukaryota.
ENOG41114G9. LUCA.
GeneTreeiENSGT00440000033589.
HOGENOMiHOG000005649.
HOVERGENiHBG099718.
InParanoidiQ8VE28.
KOiK03213.
OMAiHKRYRQK.
OrthoDBiEOG091G0HUV.
PhylomeDBiQ8VE28.
TreeFamiTF328786.

Miscellaneous databases

PROiQ8VE28.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021567.
CleanExiMM_NKD2.
GenevisibleiQ8VE28. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNKD2_MOUSE
AccessioniPrimary (citable) accession number: Q8VE28
Secondary accession number(s): Q3TYU5
, Q3UM34, Q8C4J8, Q91Y45, Q9D7U9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: September 7, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.