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Protein

Dual specificity protein phosphatase 18

Gene

Dusp18

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.1 PublicationPROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei104 – 1041Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. MAP kinase tyrosine/serine/threonine phosphatase activity Source: InterPro
  2. phosphatase activity Source: MGI
  3. protein tyrosine/serine/threonine phosphatase activity Source: GO_Central
  4. protein tyrosine phosphatase activity Source: MGI

GO - Biological processi

  1. peptidyl-tyrosine dephosphorylation Source: MGI
  2. protein localization to organelle Source: MGI
  3. protein targeting to membrane Source: MGI
  4. protein targeting to mitochondrion Source: MGI
  5. response to antibiotic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 18 (EC:3.1.3.16, EC:3.1.3.48)
Gene namesi
Name:Dusp18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:1922469. Dusp18.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus By similarity. Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication
Note: Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment.

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. extrinsic component of mitochondrial inner membrane Source: MGI
  3. intrinsic component of mitochondrial inner membrane Source: MGI
  4. mitochondrial inner membrane Source: MGI
  5. mitochondrial intermembrane space Source: MGI
  6. mitochondrion Source: MGI
  7. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 188188Dual specificity protein phosphatase 18PRO_0000094829Add
BLAST

Proteomic databases

PRIDEiQ8VE01.

PTM databases

PhosphoSiteiQ8VE01.

Expressioni

Gene expression databases

BgeeiQ8VE01.
CleanExiMM_DUSP18.
ExpressionAtlasiQ8VE01. baseline and differential.
GenevestigatoriQ8VE01.

Structurei

3D structure databases

ProteinModelPortaliQ8VE01.
SMRiQ8VE01. Positions 19-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 14970Tyrosine-protein phosphataseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni95 – 14147Sufficient for mitochondrial localizationAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ8VE01.
KOiK14165.
OMAiASPCAFP.
OrthoDBiEOG7PK90H.
PhylomeDBiQ8VE01.
TreeFamiTF316009.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VE01-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTSPWSAFPV QIPQPSIRGL SQITKSLFIS NGVAANNKLL LSSNQITTVI
60 70 80 90 100
NVSVEVANTF YEDIQYVQVP VVDAPVARLS NFFDSVADRI HSVEMQKGRT
110 120 130 140 150
LLHCAAGVSR SAALCLAYLM KYHAMSLVDA HTWTKSCRPI IRPNSGFWEQ
160 170 180
LIHYELQLFG KNTMQMMDSP MGRIPDIYEK ETRLMIPL
Length:188
Mass (Da):21,119
Last modified:March 1, 2002 - v1
Checksum:i86CB43E390DC7DE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015917 mRNA. Translation: BAC25470.1.
AK081916 mRNA. Translation: BAC38371.1.
AL731853 Genomic DNA. Translation: CAI51861.1.
BC020036 mRNA. Translation: AAH20036.1.
CCDSiCCDS24369.1.
RefSeqiNP_776106.1. NM_173745.5.
XP_006514936.1. XM_006514873.1.
UniGeneiMm.32588.

Genome annotation databases

EnsembliENSMUST00000055931; ENSMUSP00000057346; ENSMUSG00000047205.
ENSMUST00000109996; ENSMUSP00000105624; ENSMUSG00000047205.
GeneIDi75219.
KEGGimmu:75219.
UCSCiuc007htu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015917 mRNA. Translation: BAC25470.1.
AK081916 mRNA. Translation: BAC38371.1.
AL731853 Genomic DNA. Translation: CAI51861.1.
BC020036 mRNA. Translation: AAH20036.1.
CCDSiCCDS24369.1.
RefSeqiNP_776106.1. NM_173745.5.
XP_006514936.1. XM_006514873.1.
UniGeneiMm.32588.

3D structure databases

ProteinModelPortaliQ8VE01.
SMRiQ8VE01. Positions 19-179.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8VE01.

Proteomic databases

PRIDEiQ8VE01.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055931; ENSMUSP00000057346; ENSMUSG00000047205.
ENSMUST00000109996; ENSMUSP00000105624; ENSMUSG00000047205.
GeneIDi75219.
KEGGimmu:75219.
UCSCiuc007htu.2. mouse.

Organism-specific databases

CTDi150290.
MGIiMGI:1922469. Dusp18.

Phylogenomic databases

eggNOGiCOG2453.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233766.
HOVERGENiHBG051422.
InParanoidiQ8VE01.
KOiK14165.
OMAiASPCAFP.
OrthoDBiEOG7PK90H.
PhylomeDBiQ8VE01.
TreeFamiTF316009.

Miscellaneous databases

ChiTaRSiDusp18. mouse.
NextBioi342484.
PROiQ8VE01.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VE01.
CleanExiMM_DUSP18.
ExpressionAtlasiQ8VE01. baseline and differential.
GenevestigatoriQ8VE01.

Family and domain databases

Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020420. Atypical_DUSP_famB.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR020422. Dual-sp_phosphatase_subgr_cat.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01910. ADSPHPHTASEB.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary tumor.
  4. "Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane."
    Rardin M.J., Wiley S.E., Murphy A.N., Pagliarini D.J., Dixon J.E.
    J. Biol. Chem. 283:15440-15450(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiDUS18_MOUSE
AccessioniPrimary (citable) accession number: Q8VE01
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2002
Last modified: February 4, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.