Q8VDZ4 (ZDHC5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Palmitoyltransferase ZDHHC5
EC=2.3.1.-

Alternative name(s):
Zinc finger DHHC domain-containing protein 5
Short name=DHHC-5

Gene names

Name:	Zdhhc5
Synonyms:	Kiaa1748

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	715 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Palmitoyl acyltransferase for the G-protein coupled receptor SSTR5 and for FLOT2. Ref.10 Ref.11
Catalytic activity	Palmitoyl-CoA + protein-cysteine = S-palmitoyl protein + CoA.
Subcellular location	Cell membrane. Membrane; Multi-pass membrane protein Potential Ref.10.
Induction	In neural stem cells, rapid up-regulation by EGF, combined with FGF2 and heparin.
Domain	The DHHC domain is required for palmitoyltransferase activity By similarity.
Miscellaneous	In neural stem cells, rapidly degraded through the proteasome pathway following growth factors withdrawal, a strategy used to induce differentiation.
Sequence similarities	Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily. Contains 1 DHHC-type zinc finger.
Sequence caution	The sequence BAD32529.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein palmitoylation Inferred from direct assay PubMed 22325201. Source: MGI
Cellular_component	dendrite Inferred from direct assay PubMed 22325201. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.10. Source: MGI
Molecular_function	palmitoyltransferase activity Inferred from direct assay PubMed 22325201. Source: MGI zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VDZ4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VDZ4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     641-652: KAPSGVSETEEV → NPHLVSQRQRK
     653-715: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 715	715	Palmitoyltransferase ZDHHC5		PRO_0000212869
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 34	21	Helical; Potential
	Topological domain	35 – 52	18	Extracellular Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Topological domain	74 – 148	75	Cytoplasmic Potential
	Transmembrane	149 – 169	21	Helical; Potential
	Topological domain	170 – 191	22	Extracellular Potential
	Transmembrane	192 – 212	21	Helical; Potential
	Topological domain	213 – 715	503	Cytoplasmic Potential
	Zinc finger	104 – 154	51	DHHC-type
Sites
	Active site	134	1	S-palmitoyl cysteine intermediate
Amino acid modifications
	Modified residue	91	1	Phosphotyrosine Ref.5
	Modified residue	247	1	Phosphoserine Ref.6
	Modified residue	296	1	Phosphoserine Ref.7 Ref.9
	Modified residue	299	1	Phosphoserine Ref.7 Ref.9
	Modified residue	345	1	Phosphoserine Ref.7
	Modified residue	348	1	Phosphothreonine By similarity
	Modified residue	380	1	Phosphoserine Ref.8 Ref.9
	Modified residue	385	1	Phosphothreonine Ref.7
	Modified residue	409	1	Phosphoserine By similarity
	Modified residue	432	1	Phosphoserine Ref.8
	Modified residue	436	1	Phosphothreonine By similarity
	Modified residue	529	1	Phosphoserine Ref.9
	Modified residue	554	1	Phosphoserine By similarity
	Modified residue	621	1	Phosphoserine Ref.9
	Modified residue	659	1	Phosphothreonine By similarity
	Modified residue	684	1	Phosphoserine By similarity
	Modified residue	694	1	Phosphoserine Ref.6
Natural variations
	Alternative sequence	641 – 652	12	KAPSG…ETEEV → NPHLVSQRQRK in isoform 2.		VSP_006936
	Alternative sequence	653 – 715	63	Missing in isoform 2.		VSP_006937
Experimental info
	Mutagenesis	134	1	C → S: Loss of activity. Ref.11

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 6FD8E83FA3D3F961	FASTA	715	77,501
	10 20 30 40 50 60 MPAESGKRFK PSKYVPVSAA AIFLVGATTL FFAFTCPGLS LNVSPAVPIY NAIMFLFVLA 70 80 90 100 110 120 NFSMATFMDP GIFPRAEEDE DKEDDFRAPL YKTVEIKGIQ VRMKWCATCR FYRPPRCSHC 130 140 150 160 170 180 SVCDNCVEEF DHHCPWVNNC IGRRNYRYFF LFLLSLTAHI MGVFGFGLLY VLYHIEELSG 190 200 210 220 230 240 VRTAVTMAVM CVAGLFFIPV AGLTGFHVVL VARGRTTNEQ VTGKFRGGVN PFTNGCCNNV 250 260 270 280 290 300 SRVLCSSPAP RYLGRPKKEK TIVIRPPFLR PEVSDGQITV KIMDNGIQGE LRRTKSKGSL 310 320 330 340 350 360 EITESQSADA EPPPPPKPDL SRYTGLRTHL SLATNEDSSL LGKDSPPTPT MYKYRPGYSS 370 380 390 400 410 420 SSTSAAMPHS SSAKLSRGDS LKEPTSIADS SRHPSYRSEP SLEPESFRSP TFGKSFHFDP 430 440 450 460 470 480 LSSGSRSSSL KSAQGTGFEL GQLQSIRSEG TTSTSYKSLA NQTRNGSLSY DSLLTPSDSP 490 500 510 520 530 540 DFESVQAGPE PDPPLGYTSP FLSARLAQQR EAERHPRLLP TGPPHREPSP VRYDNLSRHI 550 560 570 580 590 600 VASLQEREKL LRQSPPLAGR EEEPGLGDSG IQSTPGSGHA PRTSSSSDDS KRSPLSKTPL 610 620 630 640 650 660 GRPAVPRFGK PDGLRSRGLG SPEPGTTAPY LGRSISYSSQ KAPSGVSETE EVALQPLLTP 670 680 690 700 710 KDEVQLKTTY SKSNGQPKSI GSASPGPGQP PLSSPTRGGV KKVSGVGGTT YEISV
	Isoform 2 [UniParc]. Checksum: E62675A11139C243	FASTA	651	71,269
	10 20 30 40 50 60 MPAESGKRFK PSKYVPVSAA AIFLVGATTL FFAFTCPGLS LNVSPAVPIY NAIMFLFVLA 70 80 90 100 110 120 NFSMATFMDP GIFPRAEEDE DKEDDFRAPL YKTVEIKGIQ VRMKWCATCR FYRPPRCSHC 130 140 150 160 170 180 SVCDNCVEEF DHHCPWVNNC IGRRNYRYFF LFLLSLTAHI MGVFGFGLLY VLYHIEELSG 190 200 210 220 230 240 VRTAVTMAVM CVAGLFFIPV AGLTGFHVVL VARGRTTNEQ VTGKFRGGVN PFTNGCCNNV 250 260 270 280 290 300 SRVLCSSPAP RYLGRPKKEK TIVIRPPFLR PEVSDGQITV KIMDNGIQGE LRRTKSKGSL 310 320 330 340 350 360 EITESQSADA EPPPPPKPDL SRYTGLRTHL SLATNEDSSL LGKDSPPTPT MYKYRPGYSS 370 380 390 400 410 420 SSTSAAMPHS SSAKLSRGDS LKEPTSIADS SRHPSYRSEP SLEPESFRSP TFGKSFHFDP 430 440 450 460 470 480 LSSGSRSSSL KSAQGTGFEL GQLQSIRSEG TTSTSYKSLA NQTRNGSLSY DSLLTPSDSP 490 500 510 520 530 540 DFESVQAGPE PDPPLGYTSP FLSARLAQQR EAERHPRLLP TGPPHREPSP VRYDNLSRHI 550 560 570 580 590 600 VASLQEREKL LRQSPPLAGR EEEPGLGDSG IQSTPGSGHA PRTSSSSDDS KRSPLSKTPL 610 620 630 640 650 GRPAVPRFGK PDGLRSRGLG SPEPGTTAPY LGRSISYSSQ NPHLVSQRQR K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A superfamily of membrane-associated DHHC type zinc finger proteins." Huang C.-H., Chen Y., Ye T. Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: C57BL/6J.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cerebellum.
[3]	"Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H. DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: Czech II and FVB/10. Tissue: Brain and Mammary gland.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-91, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-247 AND SER-694, MASS SPECTROMETRY. Tissue: Brain cortex.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296; SER-299; SER-345 AND THR-385, MASS SPECTROMETRY. Tissue: Liver.
[8]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-380 AND SER-432, MASS SPECTROMETRY. Tissue: Melanoma.
[9]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-296; SER-299; SER-380; SER-529 AND SER-621, MASS SPECTROMETRY. Tissue: Macrophage.
[10]	"Somatostatin receptor 5 is palmitoylated by the interacting ZDHHC5 palmitoyltransferase." Kokkola T., Kruse C., Roy-Pogodzik E.M., Pekkinen J., Bauch C., Honck H.H., Hennemann H., Kreienkamp H.J. FEBS Lett. 585:2665-2670(2011) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, TOPOLOGY, SUBCELLULAR LOCATION.
[11]	"DHHC5 protein palmitoylates flotillin-2 and is rapidly degraded on induction of neuronal differentiation in cultured cells." Li Y., Martin B.R., Cravatt B.F., Hofmann S.L. J. Biol. Chem. 287:523-530(2012) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF CYS-134.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY894891 mRNA. Translation: AAX73370.1. AK082513 mRNA. Translation: BAC38513.1. AK075641 mRNA. Translation: BAC35875.1. AK173251 mRNA. Translation: BAD32529.1. Different initiation. BC020051 mRNA. Translation: AAH20051.1. BC027047 mRNA. Translation: AAH27047.1. BC065155 mRNA. Translation: AAH65155.1.
IPI	IPI00124064. IPI00467627.
RefSeq	NP_659136.1. NM_144887.4.
UniGene	Mm.288508.
3D structure databases
ProteinModelPortal	Q8VDZ4.
ModBase	Search...
PTM databases
PhosphoSite	Q8VDZ4.
Proteomic databases
PaxDb	Q8VDZ4.
PRIDE	Q8VDZ4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000035840; ENSMUSP00000048198; ENSMUSG00000034075.
GeneID	228136.
KEGG	mmu:228136.
Organism-specific databases
CTD	25921.
MGI	MGI:1923573. Zdhhc5.
Rouge	Search...
Phylogenomic databases
eggNOG	COG5273.
GeneTree	ENSGT00550000074293.
HOGENOM	HOG000234346.
HOVERGEN	HBG057186.
InParanoid	Q8VDZ4.
OMA	VPRFGKP.
OrthoDB	EOG40S0FK.
Gene expression databases
ArrayExpress	Q8VDZ4.
Bgee	Q8VDZ4.
CleanEx	MM_ZDHHC5.
Genevestigator	Q8VDZ4.
GermOnline	ENSMUSG00000034075. Mus musculus.
Family and domain databases
InterPro	IPR001594. Znf_DHHC_palmitoyltrfase. [Graphical view]
Pfam	PF01529. zf-DHHC. 1 hit. [Graphical view]
PROSITE	PS50216. ZF_DHHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	ZDHHC5. mouse.
NextBio	378935.
SOURCE	Search...

Entry information

Entry name

ZDHC5_MOUSE

Accession

Primary (citable) accession number: Q8VDZ4
Secondary accession number(s): Q2TGE8, Q69ZB5, Q8R2X7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2003
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program