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Protein

Palmitoyltransferase ZDHHC5

Gene

Zdhhc5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Palmitoyl acyltransferase for the G-protein coupled receptor SSTR5 and for FLOT2.2 Publications

Miscellaneous

In neural stem cells, rapidly degraded through the proteasome pathway following growth factors withdrawal, a strategy used to induce differentiation.

Catalytic activityi

Palmitoyl-CoA + [protein]-L-cysteine = [protein]-S-palmitoyl-L-cysteine + CoA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei134S-palmitoyl cysteine intermediate1

GO - Molecular functioni

GO - Biological processi

  • protein palmitoylation Source: MGI

Keywordsi

Molecular functionAcyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.3.1.225 3474

Names & Taxonomyi

Protein namesi
Recommended name:
Palmitoyltransferase ZDHHC5 (EC:2.3.1.225)
Alternative name(s):
Zinc finger DHHC domain-containing protein 5
Short name:
DHHC-5
Gene namesi
Name:Zdhhc5
Synonyms:Kiaa1748
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1923573 Zdhhc5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Topological domaini35 – 52ExtracellularSequence analysisAdd BLAST18
Transmembranei53 – 73HelicalSequence analysisAdd BLAST21
Topological domaini74 – 148CytoplasmicSequence analysisAdd BLAST75
Transmembranei149 – 169HelicalSequence analysisAdd BLAST21
Topological domaini170 – 191ExtracellularSequence analysisAdd BLAST22
Transmembranei192 – 212HelicalSequence analysisAdd BLAST21
Topological domaini213 – 715CytoplasmicSequence analysisAdd BLAST503

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi134C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002128691 – 715Palmitoyltransferase ZDHHC5Add BLAST715

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei91PhosphotyrosineCombined sources1
Modified residuei247PhosphoserineBy similarity1
Modified residuei294PhosphothreonineCombined sources1
Modified residuei296PhosphoserineCombined sources1
Modified residuei299PhosphoserineCombined sources1
Modified residuei303PhosphothreonineCombined sources1
Modified residuei345PhosphoserineCombined sources1
Modified residuei348PhosphothreonineCombined sources1
Modified residuei350PhosphothreonineCombined sources1
Modified residuei380PhosphoserineCombined sources1
Modified residuei398PhosphoserineBy similarity1
Modified residuei406PhosphoserineBy similarity1
Modified residuei409PhosphoserineBy similarity1
Modified residuei411PhosphothreonineBy similarity1
Modified residuei415PhosphoserineBy similarity1
Modified residuei425PhosphoserineBy similarity1
Modified residuei429PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei436PhosphothreonineCombined sources1
Modified residuei529PhosphoserineBy similarity1
Modified residuei554PhosphoserineBy similarity1
Modified residuei617Omega-N-methylarginineBy similarity1
Modified residuei621PhosphoserineCombined sources1
Modified residuei659PhosphothreonineBy similarity1
Modified residuei684PhosphoserineBy similarity1
Modified residuei694PhosphoserineBy similarity1
Modified residuei697Omega-N-methylarginineCombined sources1

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Phosphoprotein

Proteomic databases

EPDiQ8VDZ4
PaxDbiQ8VDZ4
PeptideAtlasiQ8VDZ4
PRIDEiQ8VDZ4

PTM databases

iPTMnetiQ8VDZ4
PhosphoSitePlusiQ8VDZ4
SwissPalmiQ8VDZ4

Expressioni

Inductioni

In neural stem cells, rapid up-regulation by EGF, combined with FGF2 and heparin.

Gene expression databases

BgeeiENSMUSG00000034075
CleanExiMM_ZDHHC5
GenevisibleiQ8VDZ4 MM

Interactioni

Protein-protein interaction databases

IntActiQ8VDZ4, 2 interactors
MINTiQ8VDZ4
STRINGi10090.ENSMUSP00000048198

Structurei

3D structure databases

ProteinModelPortaliQ8VDZ4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 154DHHCPROSITE-ProRule annotationAdd BLAST51

Domaini

The DHHC domain is required for palmitoyltransferase activity.By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1311 Eukaryota
COG5273 LUCA
GeneTreeiENSGT00550000074293
HOGENOMiHOG000234346
HOVERGENiHBG057186
InParanoidiQ8VDZ4
KOiK20030
OMAiYSSQKAQ
OrthoDBiEOG091G02XW
PhylomeDBiQ8VDZ4
TreeFamiTF354263

Family and domain databases

InterProiView protein in InterPro
IPR001594 Palmitoyltrfase_DHHC
PfamiView protein in Pfam
PF01529 DHHC, 1 hit
PROSITEiView protein in PROSITE
PS50216 DHHC, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VDZ4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAESGKRFK PSKYVPVSAA AIFLVGATTL FFAFTCPGLS LNVSPAVPIY
60 70 80 90 100
NAIMFLFVLA NFSMATFMDP GIFPRAEEDE DKEDDFRAPL YKTVEIKGIQ
110 120 130 140 150
VRMKWCATCR FYRPPRCSHC SVCDNCVEEF DHHCPWVNNC IGRRNYRYFF
160 170 180 190 200
LFLLSLTAHI MGVFGFGLLY VLYHIEELSG VRTAVTMAVM CVAGLFFIPV
210 220 230 240 250
AGLTGFHVVL VARGRTTNEQ VTGKFRGGVN PFTNGCCNNV SRVLCSSPAP
260 270 280 290 300
RYLGRPKKEK TIVIRPPFLR PEVSDGQITV KIMDNGIQGE LRRTKSKGSL
310 320 330 340 350
EITESQSADA EPPPPPKPDL SRYTGLRTHL SLATNEDSSL LGKDSPPTPT
360 370 380 390 400
MYKYRPGYSS SSTSAAMPHS SSAKLSRGDS LKEPTSIADS SRHPSYRSEP
410 420 430 440 450
SLEPESFRSP TFGKSFHFDP LSSGSRSSSL KSAQGTGFEL GQLQSIRSEG
460 470 480 490 500
TTSTSYKSLA NQTRNGSLSY DSLLTPSDSP DFESVQAGPE PDPPLGYTSP
510 520 530 540 550
FLSARLAQQR EAERHPRLLP TGPPHREPSP VRYDNLSRHI VASLQEREKL
560 570 580 590 600
LRQSPPLAGR EEEPGLGDSG IQSTPGSGHA PRTSSSSDDS KRSPLSKTPL
610 620 630 640 650
GRPAVPRFGK PDGLRSRGLG SPEPGTTAPY LGRSISYSSQ KAPSGVSETE
660 670 680 690 700
EVALQPLLTP KDEVQLKTTY SKSNGQPKSI GSASPGPGQP PLSSPTRGGV
710
KKVSGVGGTT YEISV
Length:715
Mass (Da):77,501
Last modified:March 1, 2002 - v1
Checksum:i6FD8E83FA3D3F961
GO
Isoform 2 (identifier: Q8VDZ4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     641-652: KAPSGVSETEEV → NPHLVSQRQRK
     653-715: Missing.

Note: No experimental confirmation available.
Show »
Length:651
Mass (Da):71,269
Checksum:iE62675A11139C243
GO

Sequence cautioni

The sequence BAD32529 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_006936641 – 652KAPSG…ETEEV → NPHLVSQRQRK in isoform 2. 1 PublicationAdd BLAST12
Alternative sequenceiVSP_006937653 – 715Missing in isoform 2. 1 PublicationAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY894891 mRNA Translation: AAX73370.1
AK082513 mRNA Translation: BAC38513.1
AK075641 mRNA Translation: BAC35875.1
AK173251 mRNA Translation: BAD32529.1 Different initiation.
BC020051 mRNA Translation: AAH20051.1
BC027047 mRNA Translation: AAH27047.1
BC065155 mRNA Translation: AAH65155.1
CCDSiCCDS16190.1 [Q8VDZ4-1]
RefSeqiNP_659136.1, NM_144887.4 [Q8VDZ4-1]
UniGeneiMm.288508

Genome annotation databases

EnsembliENSMUST00000035840; ENSMUSP00000048198; ENSMUSG00000034075 [Q8VDZ4-1]
GeneIDi228136
KEGGimmu:228136
UCSCiuc008kiz.2 mouse [Q8VDZ4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiZDHC5_MOUSE
AccessioniPrimary (citable) accession number: Q8VDZ4
Secondary accession number(s): Q2TGE8, Q69ZB5, Q8R2X7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2003
Last sequence update: March 1, 2002
Last modified: May 23, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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