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Q8VDU0 (GPSM2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
G-protein-signaling modulator 2
Alternative name(s):
Pins homolog
Gene names
Name:Gpsm2
Synonyms:Lgn, Pins
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length679 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays an important role in spindle pole orientation By similarity. Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions.

Subunit structure

Interacts with LLGL2 By similarity. Interacts with INSC/inscuteable and F2RL2. Ref.4

Subcellular location

Cytoplasm By similarity. Cytoplasmcell cortex By similarity. Note: Localizes in the cytoplasm in the interphase and at cell periphery in the metaphase By similarity.

Tissue specificity

Expressed in many tissues, but its expression is enriched in the ventricular zone of the developing central nervous systems.

Sequence similarities

Belongs to the GPSM family.

Contains 4 GoLoco domains.

Contains 8 TPR repeats.

Caution

It is uncertain whether Met-1 or Met-8 is the initiator.

Sequence caution

The sequence AAH21308.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

The sequence AAL87447.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Dlg1Q626967EBI-7575403,EBI-389325From a different organism.
DLG4P783527EBI-7575403,EBI-80389From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 679679G-protein-signaling modulator 2
PRO_0000106359

Regions

Repeat24 – 5734TPR 1
Repeat62 – 9534TPR 2
Repeat102 – 13534TPR 3
Repeat142 – 18443TPR 4
Repeat202 – 23534TPR 5
Repeat242 – 27534TPR 6
Repeat282 – 31534TPR 7
Repeat322 – 35534TPR 8
Domain490 – 51223GoLoco 1
Domain543 – 56523GoLoco 2
Domain594 – 61623GoLoco 3
Domain628 – 65023GoLoco 4

Amino acid modifications

Modified residue4081Phosphoserine Ref.5
Modified residue4841Phosphoserine By similarity
Modified residue4871Phosphothreonine By similarity
Modified residue5401Phosphoserine By similarity
Modified residue5641Phosphoserine Ref.5

Secondary structure

......................................... 679
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Q8VDU0 [UniParc].

Last modified April 20, 2010. Version 2.
Checksum: 8005F2A52FE66D77

FASTA67975,591
        10         20         30         40         50         60 
MEGNLISMRE DHSFHVRYRM EASCLELALE GERLCKSGDC RAGVSFFEAA VQVGTEDLKT 

        70         80         90        100        110        120 
LSAIYSQLGN AYFYLHDYAK ALEYHHHDLT LARTIGDQLG EAKASGNLGN TLKVLGNFDE 

       130        140        150        160        170        180 
AIVCCQRHLD ISRELNDKVG EARALYNLGN VYHAKGKSFG CPGPQDTGEF PEDVRNALQA 

       190        200        210        220        230        240 
AVDLYEENLS LVTALGDRAA QGRAFGNLGN THYLLGNFRD AVIAHEQRLL IAKEFGDKAA 

       250        260        270        280        290        300 
ERRAYSNLGN AYIFLGEFET ASEYYKKTLL LARQLKDRAV EAQSCYSLGN TYTLLQDYEK 

       310        320        330        340        350        360 
AIDYHLKHLA IAQELKDRIG EGRACWSLGN AYTALGNHDQ AMHFAEKHLE ISREVGDKSG 

       370        380        390        400        410        420 
ELTARLNLSD LQMVLGLSYS TNNSMMSENI EIDGSLHGAG AKLGRRHSME NLELMKLTPE 

       430        440        450        460        470        480 
KVPNWNSEIL AKQKPLIAKP SAKLLFVNRL KGKKYKSGSA CTKVLQDASN SVDHRAPRSQ 

       490        500        510        520        530        540 
KKISSDTIGD EGFFDLLRRF QSNRMDDQRC HLQGNCRTTS TAAASATPKL MKAPSVSVVS 

       550        560        570        580        590        600 
PNTDEFLDLL ASSQSRRLDD QRASFSNLPG LRLTKGNSPS VLERLMTNDK KEPDEDFFDI 

       610        620        630        640        650        660 
LVKCQGSRLD DQRCAPPSAA TKGPTVPDED FFSLILRSQA KRMDEQRVLL QRDPNRDSEF 

       670 
GLKELLQNNA LLEFKHSGK 

« Hide

References

« Hide 'large scale' references
[1]"A mouse homologue of Drosophila pins can asymmetrically localize and substitute for pins function in Drosophila neuroblasts."
Yu F., Morin X., Kaushik R., Bahri S., Yang X., Chia W.
J. Cell Sci. 116:887-896(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N-3.
Tissue: Mammary tumor.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-640.
Strain: C57BL/6J.
Tissue: Aorta and Vein.
[4]"Asymmetric cell divisions promote stratification and differentiation of mammalian skin."
Lechler T., Fuchs E.
Nature 437:275-280(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH INSC AND F2RL2.
Strain: CD-1.
Tissue: Epidermis.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-408 AND SER-564, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY081187 mRNA. Translation: AAL87447.1. Different initiation.
BC021308 mRNA. Translation: AAH21308.1. Different initiation.
AK040996 mRNA. Translation: BAC30775.1.
RefSeqNP_083798.2. NM_029522.2.
UniGeneMm.226941.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3RO2X-ray2.30A22-357[»]
3RO3X-ray1.10A198-357[»]
4G2VX-ray2.40A22-357[»]
4G5OX-ray2.90E/F/G/H628-653[»]
4G5QX-ray2.90E/F/G/H628-652[»]
4G5RX-ray3.48E/F/G/Z628-652[»]
4G5SX-ray3.62E/F/G/Z594-618[»]
4JHRX-ray2.80A/B96-651[»]
ProteinModelPortalQ8VDU0.
SMRQ8VDU0. Positions 20-648.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid217976. 1 interaction.
DIPDIP-60164N.
IntActQ8VDU0. 3 interactions.
MINTMINT-4096561.

PTM databases

PhosphoSiteQ8VDU0.

Proteomic databases

PaxDbQ8VDU0.
PRIDEQ8VDU0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029482; ENSMUSP00000029482; ENSMUSG00000027883.
GeneID76123.
KEGGmmu:76123.
UCSCuc008qzo.2. mouse.

Organism-specific databases

CTD29899.
MGIMGI:1923373. Gpsm2.

Phylogenomic databases

eggNOGCOG0457.
GeneTreeENSGT00530000063126.
HOGENOMHOG000231543.
HOVERGENHBG051823.
InParanoidQ8VDU0.
KOK15837.
OMAGTEDLQV.
OrthoDBEOG7WHH8W.
PhylomeDBQ8VDU0.
TreeFamTF328344.

Gene expression databases

ArrayExpressQ8VDU0.
BgeeQ8VDU0.
GenevestigatorQ8VDU0.

Family and domain databases

Gene3D1.25.40.10. 2 hits.
InterProIPR024807. G_prot_signal_mod_2.
IPR003109. GoLoco_motif.
IPR013026. TPR-contain_dom.
IPR011990. TPR-like_helical.
IPR001440. TPR_1.
IPR019734. TPR_repeat.
[Graphical view]
PANTHERPTHR10098:SF39. PTHR10098:SF39. 1 hit.
PfamPF02188. GoLoco. 4 hits.
PF00515. TPR_1. 2 hits.
PF13176. TPR_7. 1 hit.
[Graphical view]
SMARTSM00390. GoLoco. 4 hits.
SM00028. TPR. 6 hits.
[Graphical view]
PROSITEPS50877. GOLOCO. 4 hits.
PS50005. TPR. 6 hits.
PS50293. TPR_REGION. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio344635.
PROQ8VDU0.
SOURCESearch...

Entry information

Entry nameGPSM2_MOUSE
AccessionPrimary (citable) accession number: Q8VDU0
Secondary accession number(s): Q8BLX3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: April 20, 2010
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot