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Protein

Chromobox protein homolog 7

Gene

Cbx7

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:16537902, PubMed:22226355). PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Promotes histone H3 trimethylation at 'Lys-9' (H3K9me3) (By similarity). Binds to histone H3 trimethylated at 'Lys-9' (H3K9me3) or at 'Lys-27' (H3K27me3) (PubMed:16537902, PubMed:22226355). Trimethylation at 'Lys-27' (H3K27me3) is important for chromatin recruitment (PubMed:22226355, PubMed:16537902). May possibly also bind trimethylated lysine residues in other proteins (in vitro) (PubMed:16537902). Binds non-coding, single-stranded RNA and double-stranded RNA (PubMed:20541999, PubMed:16537902). Plays a role in the timely repression of differentiation-specific genes in pluripotent embryonic stem cells to maintain the undifferentiated state (PubMed:22226355). Regulator of cellular lifespan by maintaining the repression of CDKN2A, but not by inducing telomerase activity (PubMed:14647293).By similarity4 Publications

GO - Molecular functioni

  • chromatin binding Source: MGI
  • methylated histone binding Source: UniProtKB
  • single-stranded RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Repressor, RNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Chromobox protein homolog 7
Gene namesi
Name:Cbx7
Synonyms:D15Ertd417e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1196439. Cbx7.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi11F → A: Abolishes binding to trimethylated histone H3. 1 Publication1
Mutagenesisi17R → A or Q: Strongly reduced RNA binding. Prevents cellular senescence and promotes continued cell division. 1 Publication1
Mutagenesisi31K → A: Strongly reduced RNA binding. 1 Publication1
Mutagenesisi35W → A: Strongly reduced binding to methylated histone H3 (H3K27me3). Causes premature cellular senescence. 2 Publications1

Chemistry databases

ChEMBLiCHEMBL3826868.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000802131 – 158Chromobox protein homolog 7Add BLAST158

Proteomic databases

MaxQBiQ8VDS3.
PaxDbiQ8VDS3.
PRIDEiQ8VDS3.

PTM databases

iPTMnetiQ8VDS3.
PhosphoSitePlusiQ8VDS3.

Expressioni

Tissue specificityi

Expressed in embryonic stem cells.2 Publications

Developmental stagei

In embryonic fibroblast cultured in vitro, expression gradually decreases with passage number and totally disappears in senescent cells.

Gene expression databases

BgeeiENSMUSG00000053411.
CleanExiMM_CBX7.
ExpressionAtlasiQ8VDS3. baseline and differential.
GenevisibleiQ8VDS3. MM.

Interactioni

Subunit structurei

Component of a PRC1-like complex (PubMed:22226355). Distinct PRC1-like core complexes are composed of a RING1 subunit (RING1B or RING1A), one of the six PCGF proteins (PCGF1-6), one PHC protein (PHC1-3) and one of the CBX proteins (CBX2, CBX4, CBX6, CBX7 or CBX8) (PubMed:22226355). The composition of the PRC1 complex may differ between the PRC1 complex in pluripotent embryonic stem cells containing RNF2, CBX7 and PCGF2, and the PRC1 complex in differentiating cells containing RNF2, CBX2, CBX4 and BMI1 (PubMed:22226355). Interacts with RING1 (PubMed:14647293). Interacts with RNF2, PHC1 and PCGF2 (PubMed:22226355). Interacts (via chromodomain) with histone H3K9Me3 and H3K27me3 (PubMed:16537902). Interacts with H3K9Me2 and H4K20Me1 (PubMed:16537902). Interacts (via chromodomain) with single-stranded and double-stranded RNA; RNA binding seems to be required for the localization to chromatin (PubMed:16537902). Interacts with PCGF1, PCGF3, PCGF5 and PCGF6 (By similarity).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • methylated histone binding Source: UniProtKB

Protein-protein interaction databases

BioGridi206690. 27 interactors.
DIPiDIP-38616N.
IntActiQ8VDS3. 30 interactors.
STRINGi10090.ENSMUSP00000105244.

Structurei

Secondary structure

1158
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi10 – 22Combined sources13
Beta strandi25 – 32Combined sources8
Helixi37 – 39Combined sources3
Beta strandi41 – 44Combined sources4
Helixi45 – 47Combined sources3
Helixi51 – 65Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KVMNMR-A1-71[»]
4X3KX-ray1.45A/B7-66[»]
4X3SX-ray1.60A/B7-66[»]
4X3TX-ray2.14A/B/C/D/E/F7-66[»]
4X3UX-ray1.63A/B7-66[»]
5EJWX-ray2.60A1-71[»]
ProteinModelPortaliQ8VDS3.
SMRiQ8VDS3.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VDS3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 69ChromoPROSITE-ProRule annotationAdd BLAST59

Phylogenomic databases

eggNOGiKOG2748. Eukaryota.
ENOG410ZMH7. LUCA.
GeneTreeiENSGT00530000063056.
HOGENOMiHOG000233642.
HOVERGENiHBG019320.
InParanoidiQ8VDS3.
KOiK11454.
PhylomeDBiQ8VDS3.
TreeFamiTF106456.

Family and domain databases

CDDicd00024. CHROMO. 1 hit.
InterProiView protein in InterPro
IPR033773. CBX7_C.
IPR016197. Chromo-like_dom_sf.
IPR000953. Chromo/chromo_shadow_dom.
IPR017984. Chromo_dom_subgr.
IPR023780. Chromo_domain.
IPR023779. Chromodomain_CS.
PfamiView protein in Pfam
PF17218. CBX7_C. 1 hit.
PF00385. Chromo. 1 hit.
PRINTSiPR00504. CHROMODOMAIN.
SMARTiView protein in SMART
SM00298. CHROMO. 1 hit.
SUPFAMiSSF54160. SSF54160. 1 hit.
PROSITEiView protein in PROSITE
PS00598. CHROMO_1. 1 hit.
PS50013. CHROMO_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VDS3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELSAIGEQV FAVESIRKKR VRKGKVEYLV KWKGWPPKYS TWEPEEHILD
60 70 80 90 100
PRLVMAYEEK EERDRASGYR KRGPKPRRLL LQESAAPDVV QTPGDWEPME
110 120 130 140 150
QAPEEEAEAD LTNGPPPWTP TLPSSEVTVT DITANSVTVT FREAQAAEGF

FRDRNEKL
Length:158
Mass (Da):18,109
Last modified:March 1, 2002 - v1
Checksum:iA40021F05B16BEAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY453793 mRNA. Translation: AAR26721.1.
BC021398 mRNA. Translation: AAH21398.1.
CCDSiCCDS37143.1.
RefSeqiNP_659060.1. NM_144811.3.
UniGeneiMm.323442.

Genome annotation databases

EnsembliENSMUST00000109615; ENSMUSP00000105244; ENSMUSG00000053411.
ENSMUST00000109616; ENSMUSP00000105245; ENSMUSG00000053411.
GeneIDi52609.
KEGGimmu:52609.
UCSCiuc007wuv.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiCBX7_MOUSE
AccessioniPrimary (citable) accession number: Q8VDS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: March 1, 2002
Last modified: November 22, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references