Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dedicator of cytokinesis protein 6

Gene

Dock6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as guanine nucleotide exchange factor (GEF) for CDC42 and RAC1 small GTPases (By similarity). Through its activation of CDC42 and RAC1, regulates neurite outgrowth in an vitro differentiation system.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Names & Taxonomyi

Protein namesi
Recommended name:
Dedicator of cytokinesis protein 6
Gene namesi
Name:Dock6
Synonyms:Kiaa1395
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1914789. Dock6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20802080Dedicator of cytokinesis protein 6PRO_0000189994Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei178 – 1781PhosphoserineCombined sources
Modified residuei870 – 8701PhosphoserineCombined sources
Modified residuei878 – 8781PhosphoserineCombined sources
Modified residuei882 – 8821PhosphoserineCombined sources
Modified residuei1341 – 13411PhosphoserineBy similarity
Modified residuei2064 – 20641PhosphothreonineCombined sources
Modified residuei2065 – 20651PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VDR9.
MaxQBiQ8VDR9.
PaxDbiQ8VDR9.
PRIDEiQ8VDR9.

PTM databases

iPTMnetiQ8VDR9.
PhosphoSiteiQ8VDR9.

Expressioni

Tissue specificityi

Widely expressed with highest levels in lung and heart.1 Publication

Developmental stagei

Expressed at 9.5 dpc in the growing edge of the limb buds and in the developing heart. At 10.5 dpc, strongly expressed at the edge of the limb buds, while expression in the heart maintained. At 11.5 dpc, detected in the apical ectodermal ridge of all 4 limbs, with higher expression in hindlimbs than in forelimbs. By 12.5 and 13.5 dpc, expression pattern more diffused in the limbs. At 13.5 dpc, clearly observed in the developing digits.1 Publication

Inductioni

Up-regulated during differentiation of the N1E-115 neuroblastoma cell line.1 Publication

Gene expression databases

CleanExiMM_DOCK6.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034728.

Structurei

3D structure databases

ProteinModelPortaliQ8VDR9.
SMRiQ8VDR9. Positions 1781-2053.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini546 – 712167DHR-1PROSITE-ProRule annotationAdd
BLAST
Domaini1620 – 2056437DHR-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The DHR-2 domain may mediate some GEF activity.By similarity

Sequence similaritiesi

Belongs to the DOCK family.PROSITE-ProRule annotation
Contains 1 DHR-1 domain.PROSITE-ProRule annotation
Contains 1 DHR-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1997. Eukaryota.
ENOG410XNVY. LUCA.
HOGENOMiHOG000230910.
HOVERGENiHBG051390.
InParanoidiQ8VDR9.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR026798. DOCK6.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 3 hits.
PTHR23317:SF65. PTHR23317:SF65. 3 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VDR9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASERRAFA HKINRTVAAE VRKQVSRERS GSPHSSRRSS SSLGVPLTEV
60 70 80 90 100
IEPLDFEDVL LSRPPEVEPG PLRDLIEFPV DDLELLKQPR ECRTTESGVP
110 120 130 140 150
EDGQLDAQVR AAVEMYSEDW VIVRRRYQHL STAYSPITTE TQREWQKGLT
160 170 180 190 200
CQVFEQDTPG DERTGPEDVD DPQHCSGSPE DTPRSSGASG IFSLRNLAAD
210 220 230 240 250
SLLPTLLEQA APEDVDRRNE ALRRQHRAPT LLTLYPAPDE DEAVERCSRP
260 270 280 290 300
EPPREHFGQR ILVKCLSLKF EIEIEPIFGT LALYDVREKK KISENFYFDL
310 320 330 340 350
NSDSVKGLLR AHGTHPAIST LARSAIFSVT YPSPDIFLVV KLEKVLQQGD
360 370 380 390 400
ISECCEPYMV MKEADTAKNK EKLEKLRLAA EQFCTRLGRY RMPFAWTAVH
410 420 430 440 450
LANIVSRPQD RDSDSEGERR PTWAERRRRG PQDRGYSGDD ACSFSSFRPA
460 470 480 490 500
TLTVTNFFKQ EAERLSDEDL FKFLADMRRP SSLLRRLRPV TAQLKLDISP
510 520 530 540 550
APENLHFCLS PDLLHVKPYP DPRGRPTKEI LEFPAREVYA PHSCYRNLLF
560 570 580 590 600
VYPHSLNFSS RQGSVRNLAV RIQYMAGEDQ SQALPVIFGK SSCSEFTREA
610 620 630 640 650
FTPVVYHNKS PEFYEEFKLR LPACVTENHH LFFTFYHVSC QPRPGTALET
660 670 680 690 700
PVGFTWIPLL QHGRLRTGPF CLPVSVDQPP PSYSVLTPDV ALPGMRWVDG
710 720 730 740 750
HKGVFSVELT AVSSVHPQDP HLDKFFTLVH VLEEGIFPFR LKETVLSEGT
760 770 780 790 800
MEQELRASLA ALRLASPEPL VAFSHLVLDK LVRLVVRPPI ICGQMVNLGR
810 820 830 840 850
GAFEAMAHVA SLVHRNLEAV QDSRGHCPLL ASYVHYAFRL PGGDLSLPGE
860 870 880 890 900
APPATVQAAT LARGSGRPAS LYLARSKSIS SSNPDLAVVP GSVDDEVSRI
910 920 930 940 950
LASKGVDRSH SWVNSAYAPG GSKAVLRRVP PYCGADPRQL LHEELALQWV
960 970 980 990 1000
VSGSAVRELV LQHAWFFFQL MVKSMELHLL LGQRLDTPRK LRFPGRFLDD
1010 1020 1030 1040 1050
IAALVASVGL EVITRVHKDM KLAERLNASL AFFLSDLLSI ADRGYIFSLV
1060 1070 1080 1090 1100
RAHYKQVATR LQSAPNPTAL LTLRMDFTRI LCSHEHYVTL NLPCCPLSPP
1110 1120 1130 1140 1150
ASPSPSVSST TSQSSTFSSQ APDPKVTSMF ELSGPFRQQH FLSGLLLTEL
1160 1170 1180 1190 1200
ALALDPEAEG ASLLHKKAIS AVHSLLCSHD VDSRYAEATV KAKVAELYLP
1210 1220 1230 1240 1250
LLSLARDTLP QLHGFAEGSG QRSRLASMLD SDTEGEGDIG STINPSVAMA
1260 1270 1280 1290 1300
IAGGPLAPGS RTSISQGPST AARSGCPLSA ESSRTLLVCV LWVLKNAEPT
1310 1320 1330 1340 1350
LLQRWAADLA LPQLGRLLDL LYLCLAAFEY KGKKAFERIN SLTFKKSLDM
1360 1370 1380 1390 1400
KARLEEAILG TIGARQEMVR RSRERSPFGN QENVRWRKSA THWRQTSDRV
1410 1420 1430 1440 1450
DKTKDEMEHE ALVDGNLATE ASLVVLDTLE TIVQTVMLSE ARESILSAVL
1460 1470 1480 1490 1500
KVVLYSLGSA QSALFLQHGL ATQRALVSKF PELLFEEDTE LCADLCLRLL
1510 1520 1530 1540 1550
RHCGSRISTI RMHASASLYL LMRQNFEIGH NFARVKMLVT MSLSSLVGTT
1560 1570 1580 1590 1600
QNFSEEHLRK SLKTILTYAE EDIGLRDSTF AEQVQDLMFN LHMILTDTVK
1610 1620 1630 1640 1650
MKEHQEDPEM LMDLMYRIAR GYQGSPDLRL TWLQNMAGKH AELGNHAEAA
1660 1670 1680 1690 1700
QCMVHAAALV AEYLALLEDS RHLPVGCVSF QNVSSNVLEE SAISDDILSP
1710 1720 1730 1740 1750
DEEGFCSGKN FTELGLVGLL EQAAGYFTMG GLYEAVNEVY KNLIPILEAH
1760 1770 1780 1790 1800
RDYKKLAAVH GKLQEAFTKI MHQSSGWERV FGTYFRVGFY GTRFGDLDEQ
1810 1820 1830 1840 1850
EFVYKEPSIT KLAEISHRLE EFYTERFGDD VVEIIKDSNP VDKSKLDPQK
1860 1870 1880 1890 1900
AYIQITYVEP HFDTYELKDR VTYFDRNYGL RAFLFCTPFT PDGRAHGELA
1910 1920 1930 1940 1950
EQHKRKTLLS TEHAFPYIKT RIRVCHREET VLTPVEVAIE DMQKKTRELA
1960 1970 1980 1990 2000
FATEQDPPDA KMLQMVLQGS VGPTVNQGPL EVAQVFLSEI PEDPKLFRHH
2010 2020 2030 2040 2050
NKLRLCFKDF CKKCEDALRK NKALIGPDQK EYHRELERHY SRLREALQPL
2060 2070 2080
LTQRLPQLLA PSSTSLRSSM NRSSFRKADL
Length:2,080
Mass (Da):233,267
Last modified:July 27, 2011 - v4
Checksum:iAC9541FB05DB78D0
GO
Isoform 2 (identifier: Q8VDR9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     905-939: Missing.
     1019-1062: DMKLAERLNA...HYKQVATRLQ → VRKDSAQGCS...WPWLDVETQL
     1063-2080: Missing.

Note: No experimental confirmation available.
Show »
Length:1,027
Mass (Da):115,582
Checksum:i95426603C75A5DCF
GO
Isoform 3 (identifier: Q8VDR9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     241-244: DEAV → VGAY
     245-2080: Missing.

Note: No experimental confirmation available.
Show »
Length:244
Mass (Da):27,270
Checksum:i4F45CF7C640A6A83
GO

Sequence cautioni

The sequence AAH21414.1 differs from that shown.Contaminating sequence. Vector contamination at the N-terminus.Curated
The sequence AAH21414.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAC37843.1 differs from that shown. Reason: Frameshift at position 160. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti145 – 1451W → R in BAE26239 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei241 – 2444DEAV → VGAY in isoform 3. 1 PublicationVSP_022257
Alternative sequencei245 – 20801836Missing in isoform 3. 1 PublicationVSP_022258Add
BLAST
Alternative sequencei905 – 93935Missing in isoform 2. 1 PublicationVSP_022259Add
BLAST
Alternative sequencei1019 – 106244DMKLA…ATRLQ → VRKDSAQGCSVVRDPVCHVG LFIHGLFLEHLWFTWPWLDV ETQL in isoform 2. 1 PublicationVSP_022260Add
BLAST
Alternative sequencei1063 – 20801018Missing in isoform 2. 1 PublicationVSP_022261Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016777 mRNA. Translation: BAB30423.2.
AK080190 mRNA. Translation: BAC37843.1. Frameshift.
AK145109 mRNA. Translation: BAE26239.1.
AC161371 Genomic DNA. No translation available.
AC166992 Genomic DNA. No translation available.
BC021414 mRNA. Translation: AAH21414.1. Different initiation.
BC043042 mRNA. Translation: AAH43042.1.
BC057368 mRNA. Translation: AAH57368.2.
RefSeqiNP_796004.2. NM_177030.3.
XP_011240856.1. XM_011242554.1. [Q8VDR9-1]
UniGeneiMm.128153.

Genome annotation databases

GeneIDi319899.
KEGGimmu:319899.
UCSCiuc009omn.1. mouse. [Q8VDR9-1]
uc009omq.1. mouse. [Q8VDR9-2]
uc009omt.1. mouse. [Q8VDR9-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK016777 mRNA. Translation: BAB30423.2.
AK080190 mRNA. Translation: BAC37843.1. Frameshift.
AK145109 mRNA. Translation: BAE26239.1.
AC161371 Genomic DNA. No translation available.
AC166992 Genomic DNA. No translation available.
BC021414 mRNA. Translation: AAH21414.1. Different initiation.
BC043042 mRNA. Translation: AAH43042.1.
BC057368 mRNA. Translation: AAH57368.2.
RefSeqiNP_796004.2. NM_177030.3.
XP_011240856.1. XM_011242554.1. [Q8VDR9-1]
UniGeneiMm.128153.

3D structure databases

ProteinModelPortaliQ8VDR9.
SMRiQ8VDR9. Positions 1781-2053.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034728.

PTM databases

iPTMnetiQ8VDR9.
PhosphoSiteiQ8VDR9.

Proteomic databases

EPDiQ8VDR9.
MaxQBiQ8VDR9.
PaxDbiQ8VDR9.
PRIDEiQ8VDR9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi319899.
KEGGimmu:319899.
UCSCiuc009omn.1. mouse. [Q8VDR9-1]
uc009omq.1. mouse. [Q8VDR9-2]
uc009omt.1. mouse. [Q8VDR9-3]

Organism-specific databases

CTDi57572.
MGIiMGI:1914789. Dock6.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG1997. Eukaryota.
ENOG410XNVY. LUCA.
HOGENOMiHOG000230910.
HOVERGENiHBG051390.
InParanoidiQ8VDR9.

Miscellaneous databases

PROiQ8VDR9.
SOURCEiSearch...

Gene expression databases

CleanExiMM_DOCK6.

Family and domain databases

InterProiIPR027007. DHR-1_domain.
IPR027357. DHR-2.
IPR026791. DOCK.
IPR026798. DOCK6.
IPR010703. DOCK_C.
IPR021816. DOCK_C/D_N.
[Graphical view]
PANTHERiPTHR23317. PTHR23317. 3 hits.
PTHR23317:SF65. PTHR23317:SF65. 3 hits.
PfamiPF06920. DHR-2. 1 hit.
PF14429. DOCK-C2. 1 hit.
PF11878. DUF3398. 1 hit.
[Graphical view]
PROSITEiPS51650. DHR_1. 1 hit.
PS51651. DHR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 3), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1871-2080 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Aorta, Mammary gland and Testis.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 746-2080 (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Brain and Mammary tumor.
  4. "Dock6, a Dock-C subfamily guanine nucleotide exchanger, has the dual specificity for Rac1 and Cdc42 and regulates neurite outgrowth."
    Miyamoto Y., Yamauchi J., Sanbe A., Tanoue A.
    Exp. Cell Res. 313:791-804(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-178; SER-870; SER-878; SER-882; THR-2064 AND SER-2065, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "Recessive mutations in DOCK6, encoding the guanidine nucleotide exchange factor DOCK6, lead to abnormal actin cytoskeleton organization and Adams-Oliver syndrome."
    Shaheen R., Faqeih E., Sunker A., Morsy H., Al-Sheddi T., Shamseldin H.E., Adly N., Hashem M., Alkuraya F.S.
    Am. J. Hum. Genet. 89:328-333(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiDOCK6_MOUSE
AccessioniPrimary (citable) accession number: Q8VDR9
Secondary accession number(s): E9QKQ0
, Q3UM59, Q6PFY0, Q8BJS1, Q9D461
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 3, 2003
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 101 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.