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Protein

Prostaglandin reductase 2

Gene

Ptgr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.1 Publication

Catalytic activityi

11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.1 Publication

Kineticsi

  1. KM=49.6 µM for 15-keto-PGE21 Publication
  2. KM=34.4 µM for 15-keto-PGE11 Publication
  3. KM=108.8 µM for 15-keto-PGF2-alpha1 Publication
  4. KM=59.2 µM for 15-keto-PGF2-beta1 Publication
  5. KM=94.6 µM for NADPH1 Publication
  1. Vmax=178.4 µmol/min/mg enzyme for 15-keto-PGE21 Publication
  2. Vmax=115.0 µmol/min/mg enzyme for 15-keto-PGE11 Publication
  3. Vmax=230.9 µmol/min/mg enzyme for 15-keto-PGF2-alpha1 Publication
  4. Vmax=206.4 µmol/min/mg enzyme for 15-keto-PGF2-beta1 Publication
  5. Vmax=144.7 µmol/min/mg enzyme for NADPH1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921NADP1 Publication
Binding sitei208 – 2081NADP1 Publication
Binding sitei231 – 2311NADP1 Publication
Binding sitei337 – 3371NADP1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi165 – 1684NADP1 Publication
Nucleotide bindingi253 – 2597NADP1 Publication
Nucleotide bindingi287 – 2893NADP1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.48. 3474.
ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
SABIO-RKQ8VDQ1.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 2 (EC:1.3.1.48)
Short name:
PRG-2
Alternative name(s):
15-oxoprostaglandin 13-reductase
Zinc-binding alcohol dehydrogenase domain-containing protein 1
Gene namesi
Name:Ptgr2
Synonyms:Zadh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1916372. Ptgr2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi259 – 2591Y → F: Significant reduction in catalytic efficiency. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 351351Prostaglandin reductase 2PRO_0000218071Add
BLAST

Proteomic databases

MaxQBiQ8VDQ1.
PaxDbiQ8VDQ1.
PeptideAtlasiQ8VDQ1.
PRIDEiQ8VDQ1.

PTM databases

iPTMnetiQ8VDQ1.
PhosphoSiteiQ8VDQ1.
SwissPalmiQ8VDQ1.

Expressioni

Tissue specificityi

Widely expressed with highest levels in adipose tissues.1 Publication

Developmental stagei

Highly expressed in the late phase of adipocyte differentiation (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000072946.
ExpressionAtlasiQ8VDQ1. baseline and differential.
GenevisibleiQ8VDQ1. MM.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

BioGridi218562. 1 interaction.
IntActiQ8VDQ1. 2 interactions.
MINTiMINT-4126675.
STRINGi10090.ENSMUSP00000115704.

Structurei

Secondary structure

1
351
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi20 – 223Combined sources
Beta strandi23 – 286Combined sources
Beta strandi38 – 4710Combined sources
Helixi51 – 555Combined sources
Beta strandi57 – 593Combined sources
Beta strandi75 – 8511Combined sources
Beta strandi95 – 11016Combined sources
Helixi111 – 1133Combined sources
Beta strandi115 – 1173Combined sources
Helixi119 – 1224Combined sources
Helixi126 – 1305Combined sources
Turni131 – 1333Combined sources
Helixi135 – 14713Combined sources
Beta strandi157 – 1626Combined sources
Helixi166 – 17712Combined sources
Beta strandi181 – 1899Combined sources
Helixi190 – 1989Combined sources
Beta strandi203 – 2075Combined sources
Helixi208 – 2103Combined sources
Helixi213 – 2208Combined sources
Beta strandi225 – 2328Combined sources
Helixi234 – 2429Combined sources
Beta strandi244 – 2529Combined sources
Helixi256 – 2583Combined sources
Helixi271 – 28010Combined sources
Beta strandi283 – 2864Combined sources
Helixi289 – 2913Combined sources
Helixi293 – 2953Combined sources
Helixi296 – 30813Combined sources
Beta strandi316 – 3205Combined sources
Helixi322 – 3243Combined sources
Helixi325 – 3339Combined sources
Beta strandi338 – 3447Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VJ1X-ray2.10A1-351[»]
2ZB3X-ray2.00A1-351[»]
ProteinModelPortaliQ8VDQ1.
SMRiQ8VDQ1. Positions 2-346.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VDQ1.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni99 – 1002Substrate bindingBy similarity
Regioni288 – 2903Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8VDQ1.
KOiK13949.
OMAiINIILPA.
OrthoDBiEOG091G0F5B.
TreeFamiTF324201.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VDQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIIQRVVLNS RPGKNGNPVA ENFRVEEFSL PDALNEGQVQ VRTLYLSVDP
60 70 80 90 100
YMRCKMNEDT GTDYLAPWQL AQVADGGGIG VVEESKHQKL TKGDFVTSFY
110 120 130 140 150
WPWQTKAILD GNGLEKVDPQ LVDGHLSYFL GAIGMPGLTS LIGVQEKGHI
160 170 180 190 200
SAGSNQTMVV SGAAGACGSL AGQIGHLLGC SRVVGICGTQ EKCLFLTSEL
210 220 230 240 250
GFDAAVNYKT GNVAEQLREA CPGGVDVYFD NVGGDISNAV ISQMNENSHI
260 270 280 290 300
ILCGQISQYS NDVPYPPPLP PAVEAIRKER NITRERFTVL NYKDKFEPGI
310 320 330 340 350
LQLSQWFKEG KLKVKETMAK GLENMGVAFQ SMMTGGNVGK QIVCISEDSS

L
Length:351
Mass (Da):38,015
Last modified:July 22, 2008 - v2
Checksum:i2766871577A8022F
GO
Isoform 2 (identifier: Q8VDQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-316: VKE → FLF
     317-351: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):34,449
Checksum:i7699BA248C3E09D0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti18 – 181P → T in BAB32284 (PubMed:16141072).Curated
Sequence conflicti31 – 311P → L in AAH21466 (PubMed:15489334).Curated
Sequence conflicti81 – 811V → I in BAE26315 (PubMed:16141072).Curated
Sequence conflicti81 – 811V → I in AAH21466 (PubMed:15489334).Curated
Sequence conflicti91 – 911T → A in BAE26315 (PubMed:16141072).Curated
Sequence conflicti91 – 911T → A in AAH21466 (PubMed:15489334).Curated
Sequence conflicti239 – 2391A → T in BAE26315 (PubMed:16141072).Curated
Sequence conflicti239 – 2391A → T in AAH21466 (PubMed:15489334).Curated
Sequence conflicti260 – 2612SN → NK in BAE26315 (PubMed:16141072).Curated
Sequence conflicti260 – 2612SN → NK in AAH21466 (PubMed:15489334).Curated
Sequence conflicti318 – 3181M → V in BAE26315 (PubMed:16141072).Curated
Sequence conflicti318 – 3181M → V in AAH21466 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei314 – 3163VKE → FLF in isoform 2. 1 PublicationVSP_013528
Alternative sequencei317 – 35135Missing in isoform 2. 1 PublicationVSP_013529Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036168 mRNA. Translation: BAC29329.1.
AK021033 mRNA. Translation: BAB32284.1.
AK145232 mRNA. Translation: BAE26315.1.
AK159932 mRNA. Translation: BAE35493.1.
AK168895 mRNA. Translation: BAE40712.1.
BC021466 mRNA. Translation: AAH21466.1.
CCDSiCCDS36490.1. [Q8VDQ1-1]
RefSeqiNP_001239554.1. NM_001252625.1. [Q8VDQ1-1]
NP_001239555.1. NM_001252626.1.
NP_084156.2. NM_029880.3. [Q8VDQ1-1]
UniGeneiMm.246127.

Genome annotation databases

EnsembliENSMUST00000123614; ENSMUSP00000115704; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000146377; ENSMUSP00000119981; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000147363; ENSMUSP00000114766; ENSMUSG00000072946. [Q8VDQ1-2]
GeneIDi77219.
KEGGimmu:77219.
UCSCiuc007oep.2. mouse. [Q8VDQ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036168 mRNA. Translation: BAC29329.1.
AK021033 mRNA. Translation: BAB32284.1.
AK145232 mRNA. Translation: BAE26315.1.
AK159932 mRNA. Translation: BAE35493.1.
AK168895 mRNA. Translation: BAE40712.1.
BC021466 mRNA. Translation: AAH21466.1.
CCDSiCCDS36490.1. [Q8VDQ1-1]
RefSeqiNP_001239554.1. NM_001252625.1. [Q8VDQ1-1]
NP_001239555.1. NM_001252626.1.
NP_084156.2. NM_029880.3. [Q8VDQ1-1]
UniGeneiMm.246127.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VJ1X-ray2.10A1-351[»]
2ZB3X-ray2.00A1-351[»]
ProteinModelPortaliQ8VDQ1.
SMRiQ8VDQ1. Positions 2-346.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218562. 1 interaction.
IntActiQ8VDQ1. 2 interactions.
MINTiMINT-4126675.
STRINGi10090.ENSMUSP00000115704.

PTM databases

iPTMnetiQ8VDQ1.
PhosphoSiteiQ8VDQ1.
SwissPalmiQ8VDQ1.

Proteomic databases

MaxQBiQ8VDQ1.
PaxDbiQ8VDQ1.
PeptideAtlasiQ8VDQ1.
PRIDEiQ8VDQ1.

Protocols and materials databases

DNASUi77219.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000123614; ENSMUSP00000115704; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000146377; ENSMUSP00000119981; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000147363; ENSMUSP00000114766; ENSMUSG00000072946. [Q8VDQ1-2]
GeneIDi77219.
KEGGimmu:77219.
UCSCiuc007oep.2. mouse. [Q8VDQ1-1]

Organism-specific databases

CTDi145482.
MGIiMGI:1916372. Ptgr2.

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8VDQ1.
KOiK13949.
OMAiINIILPA.
OrthoDBiEOG091G0F5B.
TreeFamiTF324201.

Enzyme and pathway databases

BRENDAi1.3.1.48. 3474.
ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
SABIO-RKQ8VDQ1.

Miscellaneous databases

EvolutionaryTraceiQ8VDQ1.
PROiQ8VDQ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072946.
ExpressionAtlasiQ8VDQ1. baseline and differential.
GenevisibleiQ8VDQ1. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTGR2_MOUSE
AccessioniPrimary (citable) accession number: Q8VDQ1
Secondary accession number(s): Q3TG36
, Q3ULY3, Q8BZA2, Q9D1W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 22, 2008
Last modified: September 7, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.