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Protein

Prostaglandin reductase 2

Gene

Ptgr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation.1 Publication

Catalytic activityi

11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.1 Publication

Kineticsi

  1. KM=49.6 µM for 15-keto-PGE21 Publication
  2. KM=34.4 µM for 15-keto-PGE11 Publication
  3. KM=108.8 µM for 15-keto-PGF2-alpha1 Publication
  4. KM=59.2 µM for 15-keto-PGF2-beta1 Publication
  5. KM=94.6 µM for NADPH1 Publication
  1. Vmax=178.4 µmol/min/mg enzyme for 15-keto-PGE21 Publication
  2. Vmax=115.0 µmol/min/mg enzyme for 15-keto-PGE11 Publication
  3. Vmax=230.9 µmol/min/mg enzyme for 15-keto-PGF2-alpha1 Publication
  4. Vmax=206.4 µmol/min/mg enzyme for 15-keto-PGF2-beta1 Publication
  5. Vmax=144.7 µmol/min/mg enzyme for NADPH1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192NADP1 Publication1
Binding sitei208NADP1 Publication1
Binding sitei231NADP1 Publication1
Binding sitei337NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi165 – 168NADP1 Publication4
Nucleotide bindingi253 – 259NADP1 Publication7
Nucleotide bindingi287 – 289NADP1 Publication3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.3.1.48. 3474.
ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
SABIO-RKQ8VDQ1.

Chemistry databases

SwissLipidsiSLP:000001623.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 2 (EC:1.3.1.48)
Short name:
PRG-2
Alternative name(s):
15-oxoprostaglandin 13-reductase
Zinc-binding alcohol dehydrogenase domain-containing protein 1
Gene namesi
Name:Ptgr2
Synonyms:Zadh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1916372. Ptgr2.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • extracellular exosome Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi259Y → F: Significant reduction in catalytic efficiency. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180711 – 351Prostaglandin reductase 2Add BLAST351

Proteomic databases

PaxDbiQ8VDQ1.
PeptideAtlasiQ8VDQ1.
PRIDEiQ8VDQ1.

PTM databases

iPTMnetiQ8VDQ1.
PhosphoSitePlusiQ8VDQ1.
SwissPalmiQ8VDQ1.

Expressioni

Tissue specificityi

Widely expressed with highest levels in adipose tissues.1 Publication

Developmental stagei

Highly expressed in the late phase of adipocyte differentiation (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000072946.
ExpressionAtlasiQ8VDQ1. baseline and differential.
GenevisibleiQ8VDQ1. MM.

Interactioni

Subunit structurei

Monomer.2 Publications

Protein-protein interaction databases

BioGridi218562. 1 interactor.
IntActiQ8VDQ1. 2 interactors.
MINTiMINT-4126675.
STRINGi10090.ENSMUSP00000115704.

Structurei

Secondary structure

1351
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi20 – 22Combined sources3
Beta strandi23 – 28Combined sources6
Beta strandi38 – 47Combined sources10
Helixi51 – 55Combined sources5
Beta strandi57 – 59Combined sources3
Beta strandi75 – 85Combined sources11
Beta strandi95 – 110Combined sources16
Helixi111 – 113Combined sources3
Beta strandi115 – 117Combined sources3
Helixi119 – 122Combined sources4
Helixi126 – 130Combined sources5
Turni131 – 133Combined sources3
Helixi135 – 147Combined sources13
Beta strandi157 – 162Combined sources6
Helixi166 – 177Combined sources12
Beta strandi181 – 189Combined sources9
Helixi190 – 198Combined sources9
Beta strandi203 – 207Combined sources5
Helixi208 – 210Combined sources3
Helixi213 – 220Combined sources8
Beta strandi225 – 232Combined sources8
Helixi234 – 242Combined sources9
Beta strandi244 – 252Combined sources9
Helixi256 – 258Combined sources3
Helixi271 – 280Combined sources10
Beta strandi283 – 286Combined sources4
Helixi289 – 291Combined sources3
Helixi293 – 295Combined sources3
Helixi296 – 308Combined sources13
Beta strandi316 – 320Combined sources5
Helixi322 – 324Combined sources3
Helixi325 – 333Combined sources9
Beta strandi338 – 344Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VJ1X-ray2.10A1-351[»]
2ZB3X-ray2.00A1-351[»]
ProteinModelPortaliQ8VDQ1.
SMRiQ8VDQ1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VDQ1.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 100Substrate bindingBy similarity2
Regioni288 – 290Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8VDQ1.
KOiK13949.
OMAiINIILPA.
OrthoDBiEOG091G0F5B.
TreeFamiTF324201.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VDQ1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MIIQRVVLNS RPGKNGNPVA ENFRVEEFSL PDALNEGQVQ VRTLYLSVDP
60 70 80 90 100
YMRCKMNEDT GTDYLAPWQL AQVADGGGIG VVEESKHQKL TKGDFVTSFY
110 120 130 140 150
WPWQTKAILD GNGLEKVDPQ LVDGHLSYFL GAIGMPGLTS LIGVQEKGHI
160 170 180 190 200
SAGSNQTMVV SGAAGACGSL AGQIGHLLGC SRVVGICGTQ EKCLFLTSEL
210 220 230 240 250
GFDAAVNYKT GNVAEQLREA CPGGVDVYFD NVGGDISNAV ISQMNENSHI
260 270 280 290 300
ILCGQISQYS NDVPYPPPLP PAVEAIRKER NITRERFTVL NYKDKFEPGI
310 320 330 340 350
LQLSQWFKEG KLKVKETMAK GLENMGVAFQ SMMTGGNVGK QIVCISEDSS

L
Length:351
Mass (Da):38,015
Last modified:July 22, 2008 - v2
Checksum:i2766871577A8022F
GO
Isoform 2 (identifier: Q8VDQ1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     314-316: VKE → FLF
     317-351: Missing.

Note: No experimental confirmation available.
Show »
Length:316
Mass (Da):34,449
Checksum:i7699BA248C3E09D0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti18P → T in BAB32284 (PubMed:16141072).Curated1
Sequence conflicti31P → L in AAH21466 (PubMed:15489334).Curated1
Sequence conflicti81V → I in BAE26315 (PubMed:16141072).Curated1
Sequence conflicti81V → I in AAH21466 (PubMed:15489334).Curated1
Sequence conflicti91T → A in BAE26315 (PubMed:16141072).Curated1
Sequence conflicti91T → A in AAH21466 (PubMed:15489334).Curated1
Sequence conflicti239A → T in BAE26315 (PubMed:16141072).Curated1
Sequence conflicti239A → T in AAH21466 (PubMed:15489334).Curated1
Sequence conflicti260 – 261SN → NK in BAE26315 (PubMed:16141072).Curated2
Sequence conflicti260 – 261SN → NK in AAH21466 (PubMed:15489334).Curated2
Sequence conflicti318M → V in BAE26315 (PubMed:16141072).Curated1
Sequence conflicti318M → V in AAH21466 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013528314 – 316VKE → FLF in isoform 2. 1 Publication3
Alternative sequenceiVSP_013529317 – 351Missing in isoform 2. 1 PublicationAdd BLAST35

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036168 mRNA. Translation: BAC29329.1.
AK021033 mRNA. Translation: BAB32284.1.
AK145232 mRNA. Translation: BAE26315.1.
AK159932 mRNA. Translation: BAE35493.1.
AK168895 mRNA. Translation: BAE40712.1.
BC021466 mRNA. Translation: AAH21466.1.
CCDSiCCDS36490.1. [Q8VDQ1-1]
RefSeqiNP_001239554.1. NM_001252625.1. [Q8VDQ1-1]
NP_001239555.1. NM_001252626.1.
NP_084156.2. NM_029880.3. [Q8VDQ1-1]
UniGeneiMm.246127.

Genome annotation databases

EnsembliENSMUST00000123614; ENSMUSP00000115704; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000146377; ENSMUSP00000119981; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000147363; ENSMUSP00000114766; ENSMUSG00000072946. [Q8VDQ1-2]
GeneIDi77219.
KEGGimmu:77219.
UCSCiuc007oep.2. mouse. [Q8VDQ1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK036168 mRNA. Translation: BAC29329.1.
AK021033 mRNA. Translation: BAB32284.1.
AK145232 mRNA. Translation: BAE26315.1.
AK159932 mRNA. Translation: BAE35493.1.
AK168895 mRNA. Translation: BAE40712.1.
BC021466 mRNA. Translation: AAH21466.1.
CCDSiCCDS36490.1. [Q8VDQ1-1]
RefSeqiNP_001239554.1. NM_001252625.1. [Q8VDQ1-1]
NP_001239555.1. NM_001252626.1.
NP_084156.2. NM_029880.3. [Q8VDQ1-1]
UniGeneiMm.246127.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1VJ1X-ray2.10A1-351[»]
2ZB3X-ray2.00A1-351[»]
ProteinModelPortaliQ8VDQ1.
SMRiQ8VDQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi218562. 1 interactor.
IntActiQ8VDQ1. 2 interactors.
MINTiMINT-4126675.
STRINGi10090.ENSMUSP00000115704.

Chemistry databases

SwissLipidsiSLP:000001623.

PTM databases

iPTMnetiQ8VDQ1.
PhosphoSitePlusiQ8VDQ1.
SwissPalmiQ8VDQ1.

Proteomic databases

PaxDbiQ8VDQ1.
PeptideAtlasiQ8VDQ1.
PRIDEiQ8VDQ1.

Protocols and materials databases

DNASUi77219.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000123614; ENSMUSP00000115704; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000146377; ENSMUSP00000119981; ENSMUSG00000072946. [Q8VDQ1-1]
ENSMUST00000147363; ENSMUSP00000114766; ENSMUSG00000072946. [Q8VDQ1-2]
GeneIDi77219.
KEGGimmu:77219.
UCSCiuc007oep.2. mouse. [Q8VDQ1-1]

Organism-specific databases

CTDi145482.
MGIiMGI:1916372. Ptgr2.

Phylogenomic databases

eggNOGiKOG1196. Eukaryota.
COG2130. LUCA.
GeneTreeiENSGT00390000009335.
HOGENOMiHOG000294663.
HOVERGENiHBG055024.
InParanoidiQ8VDQ1.
KOiK13949.
OMAiINIILPA.
OrthoDBiEOG091G0F5B.
TreeFamiTF324201.

Enzyme and pathway databases

BRENDAi1.3.1.48. 3474.
ReactomeiR-MMU-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
SABIO-RKQ8VDQ1.

Miscellaneous databases

EvolutionaryTraceiQ8VDQ1.
PROiQ8VDQ1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072946.
ExpressionAtlasiQ8VDQ1. baseline and differential.
GenevisibleiQ8VDQ1. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPTGR2_MOUSE
AccessioniPrimary (citable) accession number: Q8VDQ1
Secondary accession number(s): Q3TG36
, Q3ULY3, Q8BZA2, Q9D1W8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: July 22, 2008
Last modified: November 30, 2016
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.