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Protein

Sodium/potassium-transporting ATPase subunit alpha-1

Gene

Atp1a1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients (By similarity).By similarity

Catalytic activityi

ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei3764-aspartylphosphate intermediateBy similarity1
Binding sitei487ATPBy similarity1
Metal bindingi717MagnesiumBy similarity1
Metal bindingi721MagnesiumBy similarity1

GO - Molecular functioni

  • ADP binding Source: Ensembl
  • ATP binding Source: UniProtKB-KW
  • chaperone binding Source: MGI
  • phosphatase activity Source: MGI
  • potassium ion binding Source: Ensembl
  • sodium:potassium-exchanging ATPase activity Source: MGI
  • sodium ion binding Source: Ensembl
  • steroid hormone binding Source: MGI

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: GO_Central
  • cellular potassium ion homeostasis Source: MGI
  • cellular response to mechanical stimulus Source: Ensembl
  • cellular response to steroid hormone stimulus Source: MGI
  • cellular sodium ion homeostasis Source: MGI
  • membrane hyperpolarization Source: Ensembl
  • membrane repolarization Source: MGI
  • negative regulation of glucocorticoid biosynthetic process Source: MGI
  • negative regulation of heart contraction Source: MGI
  • positive regulation of heart contraction Source: MGI
  • positive regulation of striated muscle contraction Source: MGI
  • potassium ion import Source: MGI
  • potassium ion import across plasma membrane Source: Ensembl
  • regulation of blood pressure Source: MGI
  • regulation of cardiac muscle cell contraction Source: Ensembl
  • regulation of sodium ion transport Source: UniProtKB
  • regulation of the force of heart contraction Source: MGI
  • response to drug Source: MGI
  • sodium ion export from cell Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

Enzyme and pathway databases

ReactomeiR-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/potassium-transporting ATPase subunit alpha-1 (EC:3.6.3.9)
Short name:
Na(+)/K(+) ATPase alpha-1 subunit
Alternative name(s):
Sodium pump subunit alpha-1
Gene namesi
Name:Atp1a1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:88105. Atp1a1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini6 – 96CytoplasmicSequence analysisAdd BLAST91
Transmembranei97 – 117HelicalSequence analysisAdd BLAST21
Topological domaini118 – 129ExtracellularSequence analysisAdd BLAST12
Transmembranei130 – 150HelicalSequence analysisAdd BLAST21
Topological domaini151 – 291CytoplasmicSequence analysisAdd BLAST141
Transmembranei292 – 312HelicalSequence analysisAdd BLAST21
Topological domaini313 – 319ExtracellularSequence analysis7
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21
Topological domaini341 – 789CytoplasmicSequence analysisAdd BLAST449
Transmembranei790 – 810HelicalSequence analysisAdd BLAST21
Topological domaini811 – 865ExtracellularSequence analysisAdd BLAST55
Transmembranei866 – 886HelicalSequence analysisAdd BLAST21
Topological domaini887 – 915CytoplasmicSequence analysisAdd BLAST29
Transmembranei916 – 936HelicalSequence analysisAdd BLAST21
Topological domaini937 – 952ExtracellularSequence analysisAdd BLAST16
Transmembranei953 – 973HelicalSequence analysisAdd BLAST21
Topological domaini974 – 979CytoplasmicSequence analysis6
Transmembranei980 – 1000HelicalSequence analysisAdd BLAST21
Topological domaini1001 – 1023ExtracellularSequence analysisAdd BLAST23

GO - Cellular componenti

  • apical plasma membrane Source: MGI
  • basolateral plasma membrane Source: MGI
  • caveola Source: Ensembl
  • endoplasmic reticulum Source: Ensembl
  • endosome Source: Ensembl
  • extracellular exosome Source: MGI
  • extracellular vesicle Source: MGI
  • Golgi apparatus Source: Ensembl
  • integral component of membrane Source: UniProtKB
  • integral component of plasma membrane Source: GO_Central
  • intercalated disc Source: Ensembl
  • intracellular membrane-bounded organelle Source: GO_Central
  • membrane Source: MGI
  • myelin sheath Source: UniProtKB
  • plasma membrane Source: MGI
  • postsynaptic density Source: MGI
  • protein complex Source: MGI
  • sarcolemma Source: BHF-UCL
  • sodium:potassium-exchanging ATPase complex Source: MGI
  • T-tubule Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000024851 – 5By similarity5
ChainiPRO_00000024866 – 1023Sodium/potassium-transporting ATPase subunit alpha-1Add BLAST1018

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei9N6-acetyllysineCombined sources1
Modified residuei10PhosphotyrosineBy similarity1
Modified residuei16PhosphoserineBy similarity1
Modified residuei21N6-acetyllysineCombined sources1
Modified residuei40PhosphoserineBy similarity1
Modified residuei47PhosphoserineBy similarity1
Modified residuei228PhosphoserineCombined sources1
Modified residuei260PhosphotyrosineCombined sources1
Modified residuei452PhosphoserineBy similarity1
Modified residuei484PhosphoserineBy similarity1
Modified residuei542PhosphotyrosineBy similarity1
Modified residuei661N6-succinyllysineCombined sources1
Modified residuei668PhosphoserineCombined sources1
Modified residuei675PhosphoserineCombined sources1
Modified residuei943Phosphoserine; by PKABy similarity1

Post-translational modificationi

Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VDN2.
MaxQBiQ8VDN2.
PaxDbiQ8VDN2.
PeptideAtlasiQ8VDN2.
PRIDEiQ8VDN2.

PTM databases

iPTMnetiQ8VDN2.
PhosphoSitePlusiQ8VDN2.
SwissPalmiQ8VDN2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000033161.
CleanExiMM_ATP1A1.
GenevisibleiQ8VDN2. MM.

Interactioni

Subunit structurei

Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with SIK1 (By similarity). Interacts with SLC35G1 and STIM1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198243. 12 interactors.
DIPiDIP-31885N.
IntActiQ8VDN2. 25 interactors.
MINTiMINT-1797662.
STRINGi10090.ENSMUSP00000039657.

Structurei

3D structure databases

ProteinModelPortaliQ8VDN2.
SMRiQ8VDN2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni82 – 84Phosphoinositide-3 kinase bindingBy similarity3

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ8VDN2.
KOiK01539.
OMAiPVENLCF.
OrthoDBiEOG091G01BB.
PhylomeDBiQ8VDN2.
TreeFamiTF312838.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VDN2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKGVGRDKY EPAAVSEHGD KKGKKAKKER DMDELKKEVS MDDHKLSLDE
60 70 80 90 100
LHRKYGTDLS RGLTPARAAE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF
110 120 130 140 150
SMLLWIGAIL CFLAYGIRSA TEEEPPNDDL YLGVVLSAVV IITGCFSYYQ
160 170 180 190 200
EAKSSKIMES FKNMVPQQAL VIRNGEKMSI NAEDVVVGDL VEVKGGDRIP
210 220 230 240 250
ADLRIISANG CKVDNSSLTG ESEPQTRSPD FTNENPLETR NIAFFSTNCV
260 270 280 290 300
EGTARGIVVY TGDRTVMGRI ATLASGLEGG QTPIAEEIEH FIHLITGVAV
310 320 330 340 350
FLGVSFFILS LILEYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR
360 370 380 390 400
MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA
410 420 430 440 450
DTTENQSGVS FDKTSATWFA LSRIAGLCNR AVFQANQENL PILKRAVAGD
460 470 480 490 500
ASESALLKCI EVCCGSVMEM REKYSKIVEI PFNSTNKYQL SIHKNPNASE
510 520 530 540 550
PKHLLVMKGA PERILDRCSS ILLHGKEQPL DEELKDAFQN AYLELGGLGE
560 570 580 590 600
RVLGFCHLLL PDEQFPEGFQ FDTDDVNFPV DNLCFVGLIS MIDPPRAAVP
610 620 630 640 650
DAVGKCRSAG IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI
660 670 680 690 700
PVNQVNPRDA KACVVHGSDL KDMTSEELDD ILRYHTEIVF ARTSPQQKLI
710 720 730 740 750
IVEGCQRQGA IVAVTGDGVN DSPALKKADI GVAMGIVGSD VSKQAADMIL
760 770 780 790 800
LDDNFASIVT GVEEGRLIFD NLKKSIAYTL TSNIPEITPF LIFIIANIPL
810 820 830 840 850
PLGTVTILCI DLGTDMVPAI SLAYEQAESD IMKRQPRNPK TDKLVNERLI
860 870 880 890 900
SMAYGQIGMI QALGGFFTYF VILAENGFLP FHLLGIRETW DDRWVNDVED
910 920 930 940 950
SYGQQWTYEQ RKIVEFTCHT AFFVSIVVVQ WADLVICKTR RNSVFQQGMK
960 970 980 990 1000
NKILIFGLFE ETALAAFLSY CPGMGAALRM YPLKPTWWFC AFPYSLLIFV
1010 1020
YDEVRKLIIR RRPGGWVEKE TYY
Length:1,023
Mass (Da):112,982
Last modified:March 1, 2002 - v1
Checksum:i1E806D3FDFC5AD80
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC010319 mRNA. Translation: AAH10319.1.
BC021496 mRNA. Translation: AAH21496.1.
BC023794 mRNA. Translation: AAH23794.1.
BC025618 mRNA. Translation: AAH25618.1.
BC025627 mRNA. Translation: AAH25627.1.
BC025811 mRNA. Translation: AAH25811.1.
BC032187 mRNA. Translation: AAH32187.1.
BC033435 mRNA. Translation: AAH33435.1.
BC033471 mRNA. Translation: AAH33471.1.
BC042435 mRNA. Translation: AAH42435.1.
CCDSiCCDS17683.1.
RefSeqiNP_659149.1. NM_144900.2.
UniGeneiMm.193670.
Mm.280103.

Genome annotation databases

EnsembliENSMUST00000036493; ENSMUSP00000039657; ENSMUSG00000033161.
GeneIDi11928.
KEGGimmu:11928.
UCSCiuc008qrj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC010319 mRNA. Translation: AAH10319.1.
BC021496 mRNA. Translation: AAH21496.1.
BC023794 mRNA. Translation: AAH23794.1.
BC025618 mRNA. Translation: AAH25618.1.
BC025627 mRNA. Translation: AAH25627.1.
BC025811 mRNA. Translation: AAH25811.1.
BC032187 mRNA. Translation: AAH32187.1.
BC033435 mRNA. Translation: AAH33435.1.
BC033471 mRNA. Translation: AAH33471.1.
BC042435 mRNA. Translation: AAH42435.1.
CCDSiCCDS17683.1.
RefSeqiNP_659149.1. NM_144900.2.
UniGeneiMm.193670.
Mm.280103.

3D structure databases

ProteinModelPortaliQ8VDN2.
SMRiQ8VDN2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198243. 12 interactors.
DIPiDIP-31885N.
IntActiQ8VDN2. 25 interactors.
MINTiMINT-1797662.
STRINGi10090.ENSMUSP00000039657.

PTM databases

iPTMnetiQ8VDN2.
PhosphoSitePlusiQ8VDN2.
SwissPalmiQ8VDN2.

Proteomic databases

EPDiQ8VDN2.
MaxQBiQ8VDN2.
PaxDbiQ8VDN2.
PeptideAtlasiQ8VDN2.
PRIDEiQ8VDN2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036493; ENSMUSP00000039657; ENSMUSG00000033161.
GeneIDi11928.
KEGGimmu:11928.
UCSCiuc008qrj.2. mouse.

Organism-specific databases

CTDi476.
MGIiMGI:88105. Atp1a1.

Phylogenomic databases

eggNOGiKOG0203. Eukaryota.
COG0474. LUCA.
GeneTreeiENSGT00850000132256.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiQ8VDN2.
KOiK01539.
OMAiPVENLCF.
OrthoDBiEOG091G01BB.
PhylomeDBiQ8VDN2.
TreeFamiTF312838.

Enzyme and pathway databases

ReactomeiR-MMU-5578775. Ion homeostasis.
R-MMU-936837. Ion transport by P-type ATPases.

Miscellaneous databases

ChiTaRSiAtp1a1. mouse.
PROiQ8VDN2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033161.
CleanExiMM_ATP1A1.
GenevisibleiQ8VDN2. MM.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR005775. P-type_ATPase_IIC.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 1 hit.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT1A1_MOUSE
AccessioniPrimary (citable) accession number: Q8VDN2
Secondary accession number(s): Q91Z09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 5, 2004
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.