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Q8VDN2

- AT1A1_MOUSE

UniProt

Q8VDN2 - AT1A1_MOUSE

Protein

Sodium/potassium-transporting ATPase subunit alpha-1

Gene

Atp1a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients By similarity.By similarity

    Catalytic activityi

    ATP + H2O + Na+(In) + K+(Out) = ADP + phosphate + Na+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei376 – 37614-aspartylphosphate intermediateBy similarity
    Binding sitei487 – 4871ATPBy similarity
    Metal bindingi717 – 7171MagnesiumBy similarity
    Metal bindingi721 – 7211MagnesiumBy similarity

    GO - Molecular functioni

    1. ADP binding Source: Ensembl
    2. ATP binding Source: UniProtKB-KW
    3. phosphatase activity Source: MGI
    4. potassium ion binding Source: Ensembl
    5. protein binding Source: BHF-UCL
    6. sodium:potassium-exchanging ATPase activity Source: MGI
    7. sodium ion binding Source: Ensembl

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. cellular response to mechanical stimulus Source: Ensembl
    3. dephosphorylation Source: GOC
    4. membrane hyperpolarization Source: Ensembl
    5. negative regulation of glucocorticoid biosynthetic process Source: MGI
    6. negative regulation of heart contraction Source: MGI
    7. positive regulation of heart contraction Source: MGI
    8. positive regulation of striated muscle contraction Source: MGI
    9. potassium ion import Source: Ensembl
    10. regulation of blood pressure Source: MGI
    11. regulation of cardiac muscle cell contraction Source: Ensembl
    12. regulation of sodium ion transport Source: UniProtKB
    13. regulation of the force of heart contraction Source: MGI
    14. response to drug Source: MGI
    15. sodium ion transmembrane transport Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Potassium transport, Sodium transport, Sodium/potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Sodium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/potassium-transporting ATPase subunit alpha-1 (EC:3.6.3.9)
    Short name:
    Na(+)/K(+) ATPase alpha-1 subunit
    Alternative name(s):
    Sodium pump subunit alpha-1
    Gene namesi
    Name:Atp1a1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:88105. Atp1a1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Melanosome By similarity

    GO - Cellular componenti

    1. apical plasma membrane Source: MGI
    2. basolateral plasma membrane Source: MGI
    3. caveola Source: Ensembl
    4. endoplasmic reticulum Source: Ensembl
    5. endosome Source: Ensembl
    6. Golgi apparatus Source: Ensembl
    7. integral component of membrane Source: UniProtKB
    8. melanosome Source: UniProtKB-SubCell
    9. plasma membrane Source: MGI
    10. protein complex Source: MGI
    11. sarcolemma Source: BHF-UCL
    12. sodium:potassium-exchanging ATPase complex Source: Ensembl
    13. T-tubule Source: BHF-UCL

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Propeptidei1 – 55By similarityPRO_0000002485
    Chaini6 – 10231018Sodium/potassium-transporting ATPase subunit alpha-1PRO_0000002486Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei9 – 91N6-acetyllysine1 Publication
    Modified residuei10 – 101PhosphotyrosineBy similarity
    Modified residuei16 – 161PhosphoserineBy similarity
    Modified residuei21 – 211N6-acetyllysine1 Publication
    Modified residuei260 – 2601Phosphotyrosine1 Publication
    Modified residuei542 – 5421PhosphotyrosineBy similarity
    Modified residuei661 – 6611N6-succinyllysine1 Publication
    Modified residuei943 – 9431Phosphoserine; by PKABy similarity

    Post-translational modificationi

    Phosphorylation on Tyr-10 modulates pumping activity. Phosphorylation of Ser-943 by PKA modulates the response of ATP1A1 to PKC. Dephosphorylation by protein phosphatase 2A (PP2A) following increases in intracellular sodium, leading to increase catalytic activity By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiQ8VDN2.
    PaxDbiQ8VDN2.
    PRIDEiQ8VDN2.

    PTM databases

    PhosphoSiteiQ8VDN2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8VDN2.
    BgeeiQ8VDN2.
    CleanExiMM_ATP1A1.
    GenevestigatoriQ8VDN2.

    Interactioni

    Subunit structurei

    Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with SIK1 By similarity. Interacts with SLC35G1 and STIM1 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198243. 8 interactions.
    DIPiDIP-31885N.
    IntActiQ8VDN2. 22 interactions.
    MINTiMINT-1797662.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VDN2.
    SMRiQ8VDN2. Positions 30-1023.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini6 – 9691CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini118 – 12912ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini151 – 291141CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini313 – 3197ExtracellularSequence Analysis
    Topological domaini341 – 789449CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini811 – 86555ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini887 – 91529CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini937 – 95216ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini974 – 9796CytoplasmicSequence Analysis
    Topological domaini1001 – 102323ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei97 – 11721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei292 – 31221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei320 – 34021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei790 – 81021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei866 – 88621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei916 – 93621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei953 – 97321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei980 – 100021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni82 – 843Phosphoinositide-3 kinase bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    GeneTreeiENSGT00560000076866.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    InParanoidiQ8VDN2.
    KOiK01539.
    OMAiFLPTHLL.
    OrthoDBiEOG7327N0.
    PhylomeDBiQ8VDN2.
    TreeFamiTF312838.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VDN2-1 [UniParc]FASTAAdd to Basket

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    MGKGVGRDKY EPAAVSEHGD KKGKKAKKER DMDELKKEVS MDDHKLSLDE     50
    LHRKYGTDLS RGLTPARAAE ILARDGPNAL TPPPTTPEWV KFCRQLFGGF 100
    SMLLWIGAIL CFLAYGIRSA TEEEPPNDDL YLGVVLSAVV IITGCFSYYQ 150
    EAKSSKIMES FKNMVPQQAL VIRNGEKMSI NAEDVVVGDL VEVKGGDRIP 200
    ADLRIISANG CKVDNSSLTG ESEPQTRSPD FTNENPLETR NIAFFSTNCV 250
    EGTARGIVVY TGDRTVMGRI ATLASGLEGG QTPIAEEIEH FIHLITGVAV 300
    FLGVSFFILS LILEYTWLEA VIFLIGIIVA NVPEGLLATV TVCLTLTAKR 350
    MARKNCLVKN LEAVETLGST STICSDKTGT LTQNRMTVAH MWFDNQIHEA 400
    DTTENQSGVS FDKTSATWFA LSRIAGLCNR AVFQANQENL PILKRAVAGD 450
    ASESALLKCI EVCCGSVMEM REKYSKIVEI PFNSTNKYQL SIHKNPNASE 500
    PKHLLVMKGA PERILDRCSS ILLHGKEQPL DEELKDAFQN AYLELGGLGE 550
    RVLGFCHLLL PDEQFPEGFQ FDTDDVNFPV DNLCFVGLIS MIDPPRAAVP 600
    DAVGKCRSAG IKVIMVTGDH PITAKAIAKG VGIISEGNET VEDIAARLNI 650
    PVNQVNPRDA KACVVHGSDL KDMTSEELDD ILRYHTEIVF ARTSPQQKLI 700
    IVEGCQRQGA IVAVTGDGVN DSPALKKADI GVAMGIVGSD VSKQAADMIL 750
    LDDNFASIVT GVEEGRLIFD NLKKSIAYTL TSNIPEITPF LIFIIANIPL 800
    PLGTVTILCI DLGTDMVPAI SLAYEQAESD IMKRQPRNPK TDKLVNERLI 850
    SMAYGQIGMI QALGGFFTYF VILAENGFLP FHLLGIRETW DDRWVNDVED 900
    SYGQQWTYEQ RKIVEFTCHT AFFVSIVVVQ WADLVICKTR RNSVFQQGMK 950
    NKILIFGLFE ETALAAFLSY CPGMGAALRM YPLKPTWWFC AFPYSLLIFV 1000
    YDEVRKLIIR RRPGGWVEKE TYY 1023
    Length:1,023
    Mass (Da):112,982
    Last modified:March 1, 2002 - v1
    Checksum:i1E806D3FDFC5AD80
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC010319 mRNA. Translation: AAH10319.1.
    BC021496 mRNA. Translation: AAH21496.1.
    BC023794 mRNA. Translation: AAH23794.1.
    BC025618 mRNA. Translation: AAH25618.1.
    BC025627 mRNA. Translation: AAH25627.1.
    BC025811 mRNA. Translation: AAH25811.1.
    BC032187 mRNA. Translation: AAH32187.1.
    BC033435 mRNA. Translation: AAH33435.1.
    BC033471 mRNA. Translation: AAH33471.1.
    BC042435 mRNA. Translation: AAH42435.1.
    CCDSiCCDS17683.1.
    RefSeqiNP_659149.1. NM_144900.2.
    UniGeneiMm.280103.

    Genome annotation databases

    EnsembliENSMUST00000036493; ENSMUSP00000039657; ENSMUSG00000033161.
    GeneIDi11928.
    KEGGimmu:11928.
    UCSCiuc008qrj.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC010319 mRNA. Translation: AAH10319.1 .
    BC021496 mRNA. Translation: AAH21496.1 .
    BC023794 mRNA. Translation: AAH23794.1 .
    BC025618 mRNA. Translation: AAH25618.1 .
    BC025627 mRNA. Translation: AAH25627.1 .
    BC025811 mRNA. Translation: AAH25811.1 .
    BC032187 mRNA. Translation: AAH32187.1 .
    BC033435 mRNA. Translation: AAH33435.1 .
    BC033471 mRNA. Translation: AAH33471.1 .
    BC042435 mRNA. Translation: AAH42435.1 .
    CCDSi CCDS17683.1.
    RefSeqi NP_659149.1. NM_144900.2.
    UniGenei Mm.280103.

    3D structure databases

    ProteinModelPortali Q8VDN2.
    SMRi Q8VDN2. Positions 30-1023.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198243. 8 interactions.
    DIPi DIP-31885N.
    IntActi Q8VDN2. 22 interactions.
    MINTi MINT-1797662.

    PTM databases

    PhosphoSitei Q8VDN2.

    Proteomic databases

    MaxQBi Q8VDN2.
    PaxDbi Q8VDN2.
    PRIDEi Q8VDN2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000036493 ; ENSMUSP00000039657 ; ENSMUSG00000033161 .
    GeneIDi 11928.
    KEGGi mmu:11928.
    UCSCi uc008qrj.2. mouse.

    Organism-specific databases

    CTDi 476.
    MGIi MGI:88105. Atp1a1.

    Phylogenomic databases

    eggNOGi COG0474.
    GeneTreei ENSGT00560000076866.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    InParanoidi Q8VDN2.
    KOi K01539.
    OMAi FLPTHLL.
    OrthoDBi EOG7327N0.
    PhylomeDBi Q8VDN2.
    TreeFami TF312838.

    Miscellaneous databases

    ChiTaRSi ATP1A1. mouse.
    NextBioi 280013.
    PROi Q8VDN2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VDN2.
    Bgeei Q8VDN2.
    CleanExi MM_ATP1A1.
    Genevestigatori Q8VDN2.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 1 hit.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver and Mammary gland.
    2. Lubec G., Kang S.U., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Strain: C57BL/6 and OF1.
      Tissue: Brain and Hippocampus.
    3. "Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations."
      Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.
      Mol. Cell. Proteomics 6:283-293(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain cortex.
    4. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-260, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.
    5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-9 AND LYS-21, SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-661, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiAT1A1_MOUSE
    AccessioniPrimary (citable) accession number: Q8VDN2
    Secondary accession number(s): Q91Z09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 5, 2004
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3