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Protein

Zinc finger protein GLIS2

Gene

Glis2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can act either as a transcriptional repressor or as a transcriptional activator, depending on the cell context. Acts as a repressor of the Hedgehog signaling pathway. Represses the Hedgehog-dependent expression of Wnt4. Necessary to maintain the differentiated epithelial phenotype in renal cells through the inhibition of SNAI1, which itself induces the epithelial-to-mesenchymal transition. Represses transcriptional activation by CTNNB1 in the Wnt signaling pathway. May act by recruiting the corepressors CTBP1 and HDAC3. May be involved in neuron differentiation.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri168 – 193C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri202 – 229C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri235 – 257C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri263 – 287C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri293 – 317C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

GO - Molecular functioni

  • DNA binding Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • RNA polymerase II regulatory region sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: BHF-UCL
  • transcription factor activity, sequence-specific DNA binding Source: MGI
  • transcription regulatory region DNA binding Source: BHF-UCL

GO - Biological processi

  • cell differentiation involved in kidney development Source: MGI
  • kidney development Source: MGI
  • negative regulation of sequence-specific DNA binding transcription factor activity Source: BHF-UCL
  • negative regulation of smoothened signaling pathway Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • negative regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • nervous system development Source: UniProtKB-KW
  • positive regulation of protein localization to nucleus Source: MGI
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • regulation of transcription from RNA polymerase II promoter involved in kidney development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein, Repressor

Keywords - Biological processi

Differentiation, Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger protein GLIS2
Alternative name(s):
GLI-similar 2
Neuronal Krueppel-like protein
Zinc finger protein GLI5
Gene namesi
Name:Glis2
Synonyms:Gli5, Nkl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1932535. Glis2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • non-motile cilium Source: MGI
  • nuclear speck Source: UniProtKB
  • nucleus Source: BHF-UCL
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi170C → A: Impairs DNA-binding. 1 Publication1
Mutagenesisi175C → A: Abolishes interaction with CTNNB1. No effect on nuclear localization. 1 Publication1
Mutagenesisi288Y → A: No effect on C-terminus cleavage. 1 Publication1
Mutagenesisi290D → A: Impairs C-terminus cleavage. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002869841 – 521Zinc finger protein GLIS2Add BLAST521

Post-translational modificationi

C-terminus cleavage is induced by interaction with CTNND1 and enhances by Src tyrosine kinase.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei287 – 288Cleavage2

Proteomic databases

PaxDbiQ8VDL9.
PRIDEiQ8VDL9.

PTM databases

PhosphoSitePlusiQ8VDL9.

Expressioni

Tissue specificityi

Expressed at high levels in kidney, and at lower levels in heart and lung.1 Publication

Developmental stagei

Expression begins at E9.5 in cranial ganglia, dorsal root ganglia and neural tube. At E10.5, broadly expressed in the intermediate zone of the hindbrain, spinal cord and dorsal root ganglia. By E12.5, expression in the spinal cord becomes restricted to a narrow band of cells in the ventricular zone.1 Publication

Gene expression databases

BgeeiENSMUSG00000014303.
CleanExiMM_GLIS2.
ExpressionAtlasiQ8VDL9. baseline and differential.
GenevisibleiQ8VDL9. MM.

Interactioni

Subunit structurei

Interacts with CTBP1 and HDAC3. Interacts with CTNNB1 and CTNND1. Interacts with SUFU.4 Publications

Protein-protein interaction databases

BioGridi219913. 2 interactors.
STRINGi10090.ENSMUSP00000014447.

Structurei

3D structure databases

ProteinModelPortaliQ8VDL9.
SMRiQ8VDL9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 174Interaction with CTNND11 PublicationAdd BLAST140
Regioni71 – 137Transcription activationAdd BLAST67
Regioni148 – 171Transcription repressionAdd BLAST24

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi333 – 378Pro-richAdd BLAST46
Compositional biasi387 – 447Gly-richAdd BLAST61

Domaini

The C2H2-type zinc finger 1 has a major repressor function and is required for CTNNB1 binding.

Sequence similaritiesi

Contains 5 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri168 – 193C2H2-type 1PROSITE-ProRule annotationAdd BLAST26
Zinc fingeri202 – 229C2H2-type 2; atypicalPROSITE-ProRule annotationAdd BLAST28
Zinc fingeri235 – 257C2H2-type 3PROSITE-ProRule annotationAdd BLAST23
Zinc fingeri263 – 287C2H2-type 4PROSITE-ProRule annotationAdd BLAST25
Zinc fingeri293 – 317C2H2-type 5PROSITE-ProRule annotationAdd BLAST25

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000065778.
HOVERGENiHBG101807.
InParanoidiQ8VDL9.
KOiK09233.
OMAiDKCLSPE.
OrthoDBiEOG091G0IZP.
PhylomeDBiQ8VDL9.
TreeFamiTF351425.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VDL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHSLDEPLDL KLSITKLRAA REKRERTLGV VRHHALHREL GLVDDSPAPG
60 70 80 90 100
SPGSPPPGFL LNPKFPEKVD GRFSAAPLVD LSLSPPSGLD SPNGSSSLSP
110 120 130 140 150
ECQGNGDLPP LPTAVDFQPL RYLDGVPSSF QFFLPLGSGG ALHLPASSFL
160 170 180 190 200
PPPKDKCLSP ELPLAKQLVC RWAKCNQLFE LLQDLVDHVN DHHVKPEQDA
210 220 230 240 250
RYCCHWEGCA RHGRGFNARY KMLIHIRTHT NEKPHRCPTC NKSFSRLENL
260 270 280 290 300
KIHNRSHTGE KPYVCPYEGC NKRYSNSSDR FKHTRTHYVD KPYYCKMPGC
310 320 330 340 350
HKRYTDPSSL RKHIKAHGHF VSHEQQELLQ LRPPPKPPLP TPDSGSYVSG
360 370 380 390 400
AQIIIPNPAA LFGGPSLPGL PLPLPPGPLD LSALACGNGG GGGGGIGPGL
410 420 430 440 450
PGSVLPLNLA KNPLLPSPFG AGGLGLPVVS LLGGSAGSKA EGEKGRGSVP
460 470 480 490 500
ARVLGLEDHK TPLERTERSR SRPSPDGLPL LPGTVLDLST GNSAASSPEV
510 520
LTPGWVVIPP GSVLLKPAVV N
Length:521
Mass (Da):55,842
Last modified:March 1, 2002 - v1
Checksum:i6B5F78F4A742C72D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 88SG → CE in AAL93213 (PubMed:11741991).Curated2
Sequence conflicti208G → D in AAK00953 (PubMed:11741991).Curated1
Sequence conflicti210Missing in AAK28410 (PubMed:11262234).Curated1
Sequence conflicti249N → K in AAL93213 (PubMed:11741991).Curated1
Sequence conflicti315 – 318KAHG → RPW in AAL93213 (PubMed:11741991).Curated4
Sequence conflicti387G → A in AAK28410 (PubMed:11262234).Curated1
Sequence conflicti458D → N in AAL93213 (PubMed:11741991).Curated1
Sequence conflicti458D → N in AAK00953 (PubMed:11741991).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249340 mRNA. Translation: AAK28410.1.
AF325913 mRNA. Translation: AAK00953.1.
AF336135 Genomic DNA. Translation: AAL93213.1.
BC021517 mRNA. Translation: AAH21517.1.
CCDSiCCDS27920.1.
RefSeqiNP_112461.2. NM_031184.3.
XP_006522831.1. XM_006522768.3.
UniGeneiMm.134072.

Genome annotation databases

EnsembliENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303.
GeneIDi83396.
KEGGimmu:83396.
UCSCiuc007xzw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249340 mRNA. Translation: AAK28410.1.
AF325913 mRNA. Translation: AAK00953.1.
AF336135 Genomic DNA. Translation: AAL93213.1.
BC021517 mRNA. Translation: AAH21517.1.
CCDSiCCDS27920.1.
RefSeqiNP_112461.2. NM_031184.3.
XP_006522831.1. XM_006522768.3.
UniGeneiMm.134072.

3D structure databases

ProteinModelPortaliQ8VDL9.
SMRiQ8VDL9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219913. 2 interactors.
STRINGi10090.ENSMUSP00000014447.

PTM databases

PhosphoSitePlusiQ8VDL9.

Proteomic databases

PaxDbiQ8VDL9.
PRIDEiQ8VDL9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000014447; ENSMUSP00000014447; ENSMUSG00000014303.
GeneIDi83396.
KEGGimmu:83396.
UCSCiuc007xzw.1. mouse.

Organism-specific databases

CTDi84662.
MGIiMGI:1932535. Glis2.

Phylogenomic databases

eggNOGiKOG1721. Eukaryota.
COG5048. LUCA.
GeneTreeiENSGT00760000118771.
HOGENOMiHOG000065778.
HOVERGENiHBG101807.
InParanoidiQ8VDL9.
KOiK09233.
OMAiDKCLSPE.
OrthoDBiEOG091G0IZP.
PhylomeDBiQ8VDL9.
TreeFamiTF351425.

Miscellaneous databases

PROiQ8VDL9.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000014303.
CleanExiMM_GLIS2.
ExpressionAtlasiQ8VDL9. baseline and differential.
GenevisibleiQ8VDL9. MM.

Family and domain databases

Gene3Di3.30.160.60. 5 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
PfamiPF13912. zf-C2H2_6. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 5 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 4 hits.
PS50157. ZINC_FINGER_C2H2_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGLIS2_MOUSE
AccessioniPrimary (citable) accession number: Q8VDL9
Secondary accession number(s): Q8R4X9, Q99MY6, Q99P73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 15, 2007
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.