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Protein

ADP-dependent glucokinase

Gene

Adpgk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.

Catalytic activityi

ADP + D-glucose = AMP + D-glucose 6-phosphate.

Cofactori

Mg2+PROSITE-ProRule annotationNote: Binds 1 Mg2+ ion per subunit.PROSITE-ProRule annotation

Kineticsi

  1. KM=96 µM for D-glucose (at 37 degrees Celsius)1 Publication
  2. KM=0.28 mM for ADP (at 37 degrees Celsius)1 Publication
  3. KM=20 mM for ADP (at 37 degrees Celsius)1 Publication
  1. Vmax=160 µmol/min/mg enzyme with glucose and ADP as substrates (at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 5.75-6.5 and 8.75-9.0.1 Publication

Pathwayi: glycolysis

This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi297MagnesiumPROSITE-ProRule annotation1
Metal bindingi328MagnesiumPROSITE-ProRule annotation1
Active sitei481Proton acceptorPROSITE-ProRule annotation1
Metal bindingi481MagnesiumPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.1.147. 3474.
SABIO-RKQ8VDL4.
UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent glucokinase (EC:2.7.1.147)
Short name:
ADP-GK
Short name:
ADPGK
Gene namesi
Name:Adpgk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919391. Adpgk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000018477723 – 496ADP-dependent glucokinaseAdd BLAST474

Proteomic databases

PaxDbiQ8VDL4.
PeptideAtlasiQ8VDL4.
PRIDEiQ8VDL4.

PTM databases

PhosphoSitePlusiQ8VDL4.
SwissPalmiQ8VDL4.

Expressioni

Gene expression databases

BgeeiENSMUSG00000025236.
CleanExiMM_ADPGK.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiQ8VDL4. 1 interactor.
MINTiMINT-4126462.
STRINGi10090.ENSMUSP00000026266.

Structurei

Secondary structure

1496
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi53 – 64Combined sources12
Beta strandi73 – 79Combined sources7
Beta strandi82 – 88Combined sources7
Helixi89 – 95Combined sources7
Beta strandi107 – 109Combined sources3
Helixi112 – 125Combined sources14
Beta strandi129 – 133Combined sources5
Helixi136 – 148Combined sources13
Beta strandi153 – 158Combined sources6
Helixi159 – 168Combined sources10
Beta strandi173 – 177Combined sources5
Helixi183 – 188Combined sources6
Helixi197 – 199Combined sources3
Beta strandi206 – 212Combined sources7
Beta strandi227 – 232Combined sources6
Helixi235 – 238Combined sources4
Turni239 – 242Combined sources4
Helixi243 – 253Combined sources11
Beta strandi256 – 260Combined sources5
Helixi263 – 266Combined sources4
Helixi271 – 287Combined sources17
Beta strandi294 – 297Combined sources4
Helixi304 – 312Combined sources9
Helixi316 – 318Combined sources3
Beta strandi320 – 324Combined sources5
Helixi326 – 335Combined sources10
Turni339 – 342Combined sources4
Helixi352 – 366Combined sources15
Turni370 – 372Combined sources3
Beta strandi378 – 382Combined sources5
Beta strandi384 – 392Combined sources9
Beta strandi395 – 397Combined sources3
Helixi399 – 415Combined sources17
Beta strandi424 – 427Combined sources4
Beta strandi431 – 435Combined sources5
Turni437 – 440Combined sources4
Beta strandi443 – 445Combined sources3
Beta strandi453 – 456Combined sources4
Beta strandi458 – 466Combined sources9
Beta strandi468 – 472Combined sources5
Helixi479 – 489Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CCFX-ray2.10A51-496[»]
5CK7X-ray2.99A51-496[»]
ProteinModelPortaliQ8VDL4.
SMRiQ8VDL4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 496ADPKPROSITE-ProRule annotationAdd BLAST445

Sequence similaritiesi

Belongs to the ADP-dependent glucokinase family.Curated
Contains 1 ADPK (ADP-dependent kinase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4184. Eukaryota.
ENOG410Z7J9. LUCA.
HOGENOMiHOG000233731.
HOVERGENiHBG050461.
InParanoidiQ8VDL4.
KOiK08074.
PhylomeDBiQ8VDL4.
TreeFamiTF313401.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR007666. ADP_PFK/GK.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VDL4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP
60 70 80 90 100
LSPESRLAAA WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP
110 120 130 140 150
GSGKDHAILH SRSDLEEAFL YFMGKGAAAE RFFSDKETFH DIAQAASEFP
160 170 180 190 200
GAQHYVGGNA ALIGQRFAAN TDLKVLLCGP IGPKLHELLD DNVFVPPESL
210 220 230 240 250
QEEDEFHLIL EYLAGEEWGP FKAPHANRFI FSHDLSNGAM NMLEVFVSSL
260 270 280 290 300
EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTAISDIPT GIPVHLELAS
310 320 330 340 350
MTNRELMSSI VHQQVFPAVA SLGLNEQELL FLSQSASGPH SSLSSWDGVP
360 370 380 390 400
DVGMVSDILF WILKEHGRSE NRASDLTRIH FHTLVYHILA TVDGHWANQL
410 420 430 440 450
AAVAAGARVA GTQACATETI DTNRVSLRAP QEFTTSHLES GSRIVLNPDK
460 470 480 490
PVVEWHREGI TFHFTPVLVC KDPVRTVGLG DAISAEGLFY SEARPD
Length:496
Mass (Da):53,902
Last modified:April 26, 2005 - v2
Checksum:iCAFCB41D50131A54
GO
Isoform 2 (identifier: Q8VDL4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-159: HYVGGN → VCPTHQ
     160-496: Missing.

Show »
Length:159
Mass (Da):16,864
Checksum:i0910744000BDE52B
GO
Isoform 3 (identifier: Q8VDL4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     313-313: Missing.

Show »
Length:495
Mass (Da):53,774
Checksum:iB1341DD422E7076D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti318A → T in BAB27619 (PubMed:16141072).Curated1
Sequence conflicti341S → A in BAB27619 (PubMed:16141072).Curated1
Sequence conflicti373A → S in AAH21526 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_013555154 – 159HYVGGN → VCPTHQ in isoform 2. 1 Publication6
Alternative sequenceiVSP_013556160 – 496Missing in isoform 2. 1 PublicationAdd BLAST337
Alternative sequenceiVSP_013557313Missing in isoform 3. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011434 mRNA. Translation: BAB27619.1.
AK048904 mRNA. Translation: BAC33486.1.
BC021526 mRNA. Translation: AAH21526.1.
CCDSiCCDS23248.1. [Q8VDL4-1]
RefSeqiNP_082397.1. NM_028121.2.
UniGeneiMm.22456.

Genome annotation databases

GeneIDi72141.
KEGGimmu:72141.
UCSCiuc009pxo.1. mouse. [Q8VDL4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011434 mRNA. Translation: BAB27619.1.
AK048904 mRNA. Translation: BAC33486.1.
BC021526 mRNA. Translation: AAH21526.1.
CCDSiCCDS23248.1. [Q8VDL4-1]
RefSeqiNP_082397.1. NM_028121.2.
UniGeneiMm.22456.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CCFX-ray2.10A51-496[»]
5CK7X-ray2.99A51-496[»]
ProteinModelPortaliQ8VDL4.
SMRiQ8VDL4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VDL4. 1 interactor.
MINTiMINT-4126462.
STRINGi10090.ENSMUSP00000026266.

PTM databases

PhosphoSitePlusiQ8VDL4.
SwissPalmiQ8VDL4.

Proteomic databases

PaxDbiQ8VDL4.
PeptideAtlasiQ8VDL4.
PRIDEiQ8VDL4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72141.
KEGGimmu:72141.
UCSCiuc009pxo.1. mouse. [Q8VDL4-2]

Organism-specific databases

CTDi83440.
MGIiMGI:1919391. Adpgk.

Phylogenomic databases

eggNOGiKOG4184. Eukaryota.
ENOG410Z7J9. LUCA.
HOGENOMiHOG000233731.
HOVERGENiHBG050461.
InParanoidiQ8VDL4.
KOiK08074.
PhylomeDBiQ8VDL4.
TreeFamiTF313401.

Enzyme and pathway databases

UniPathwayiUPA00109.
BRENDAi2.7.1.147. 3474.
SABIO-RKQ8VDL4.

Miscellaneous databases

PROiQ8VDL4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025236.
CleanExiMM_ADPGK.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR007666. ADP_PFK/GK.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADPGK_MOUSE
AccessioniPrimary (citable) accession number: Q8VDL4
Secondary accession number(s): Q8C7Y9, Q9D0H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: November 2, 2016
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.