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Protein

ADP-dependent glucokinase

Gene

Adpgk

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.

Catalytic activityi

ADP + D-glucose = AMP + D-glucose 6-phosphate.

Cofactori

Mg2+PROSITE-ProRule annotationNote: Binds 1 Mg2+ ion per subunit.PROSITE-ProRule annotation

Kineticsi

  1. KM=96 µM for D-glucose (at 37 degrees Celsius)1 Publication
  2. KM=0.28 mM for ADP (at 37 degrees Celsius)1 Publication
  3. KM=20 mM for ADP (at 37 degrees Celsius)1 Publication
  1. Vmax=160 µmol/min/mg enzyme with glucose and ADP as substrates (at 37 degrees Celsius)1 Publication

pH dependencei

Optimum pH is 5.75-6.5 and 8.75-9.0.1 Publication

Pathwayi: glycolysis

This protein is involved in the pathway glycolysis, which is part of Carbohydrate degradation.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi297 – 2971MagnesiumPROSITE-ProRule annotation
Metal bindingi328 – 3281MagnesiumPROSITE-ProRule annotation
Active sitei481 – 4811Proton acceptorPROSITE-ProRule annotation
Metal bindingi481 – 4811MagnesiumPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi2.7.1.147. 3474.
SABIO-RKQ8VDL4.
UniPathwayiUPA00109.

Names & Taxonomyi

Protein namesi
Recommended name:
ADP-dependent glucokinase (EC:2.7.1.147)
Short name:
ADP-GK
Short name:
ADPGK
Gene namesi
Name:Adpgk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1919391. Adpgk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 496474ADP-dependent glucokinasePRO_0000184777Add
BLAST

Proteomic databases

EPDiQ8VDL4.
MaxQBiQ8VDL4.
PaxDbiQ8VDL4.
PRIDEiQ8VDL4.

PTM databases

PhosphoSiteiQ8VDL4.
SwissPalmiQ8VDL4.

Expressioni

Gene expression databases

BgeeiQ8VDL4.
CleanExiMM_ADPGK.

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

IntActiQ8VDL4. 1 interaction.
MINTiMINT-4126462.
STRINGi10090.ENSMUSP00000026266.

Structurei

Secondary structure

1
496
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi53 – 6412Combined sources
Beta strandi73 – 797Combined sources
Beta strandi82 – 887Combined sources
Helixi89 – 957Combined sources
Beta strandi107 – 1093Combined sources
Helixi112 – 12514Combined sources
Beta strandi129 – 1335Combined sources
Helixi136 – 14813Combined sources
Beta strandi153 – 1586Combined sources
Helixi159 – 16810Combined sources
Beta strandi173 – 1775Combined sources
Helixi183 – 1886Combined sources
Helixi197 – 1993Combined sources
Beta strandi206 – 2127Combined sources
Beta strandi227 – 2326Combined sources
Helixi235 – 2384Combined sources
Turni239 – 2424Combined sources
Helixi243 – 25311Combined sources
Beta strandi256 – 2605Combined sources
Helixi263 – 2664Combined sources
Helixi271 – 28717Combined sources
Beta strandi294 – 2974Combined sources
Helixi304 – 3129Combined sources
Helixi316 – 3183Combined sources
Beta strandi320 – 3245Combined sources
Helixi326 – 33510Combined sources
Turni339 – 3424Combined sources
Helixi352 – 36615Combined sources
Turni370 – 3723Combined sources
Beta strandi378 – 3825Combined sources
Beta strandi384 – 3929Combined sources
Beta strandi395 – 3973Combined sources
Helixi399 – 41517Combined sources
Beta strandi424 – 4274Combined sources
Beta strandi431 – 4355Combined sources
Turni437 – 4404Combined sources
Beta strandi443 – 4453Combined sources
Beta strandi453 – 4564Combined sources
Beta strandi458 – 4669Combined sources
Beta strandi468 – 4725Combined sources
Helixi479 – 48911Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5CCFX-ray2.10A51-496[»]
5CK7X-ray2.99A51-496[»]
ProteinModelPortaliQ8VDL4.
SMRiQ8VDL4. Positions 109-488.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 496445ADPKPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ADP-dependent glucokinase family.Curated
Contains 1 ADPK (ADP-dependent kinase) domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4184. Eukaryota.
ENOG410Z7J9. LUCA.
HOGENOMiHOG000233731.
HOVERGENiHBG050461.
InParanoidiQ8VDL4.
KOiK08074.
OrthoDBiEOG7V1FRC.
PhylomeDBiQ8VDL4.
TreeFamiTF313401.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR007666. ADP_PFK/GK.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VDL4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP
60 70 80 90 100
LSPESRLAAA WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP
110 120 130 140 150
GSGKDHAILH SRSDLEEAFL YFMGKGAAAE RFFSDKETFH DIAQAASEFP
160 170 180 190 200
GAQHYVGGNA ALIGQRFAAN TDLKVLLCGP IGPKLHELLD DNVFVPPESL
210 220 230 240 250
QEEDEFHLIL EYLAGEEWGP FKAPHANRFI FSHDLSNGAM NMLEVFVSSL
260 270 280 290 300
EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTAISDIPT GIPVHLELAS
310 320 330 340 350
MTNRELMSSI VHQQVFPAVA SLGLNEQELL FLSQSASGPH SSLSSWDGVP
360 370 380 390 400
DVGMVSDILF WILKEHGRSE NRASDLTRIH FHTLVYHILA TVDGHWANQL
410 420 430 440 450
AAVAAGARVA GTQACATETI DTNRVSLRAP QEFTTSHLES GSRIVLNPDK
460 470 480 490
PVVEWHREGI TFHFTPVLVC KDPVRTVGLG DAISAEGLFY SEARPD
Length:496
Mass (Da):53,902
Last modified:April 26, 2005 - v2
Checksum:iCAFCB41D50131A54
GO
Isoform 2 (identifier: Q8VDL4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     154-159: HYVGGN → VCPTHQ
     160-496: Missing.

Show »
Length:159
Mass (Da):16,864
Checksum:i0910744000BDE52B
GO
Isoform 3 (identifier: Q8VDL4-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     313-313: Missing.

Show »
Length:495
Mass (Da):53,774
Checksum:iB1341DD422E7076D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti318 – 3181A → T in BAB27619 (PubMed:16141072).Curated
Sequence conflicti341 – 3411S → A in BAB27619 (PubMed:16141072).Curated
Sequence conflicti373 – 3731A → S in AAH21526 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei154 – 1596HYVGGN → VCPTHQ in isoform 2. 1 PublicationVSP_013555
Alternative sequencei160 – 496337Missing in isoform 2. 1 PublicationVSP_013556Add
BLAST
Alternative sequencei313 – 3131Missing in isoform 3. 1 PublicationVSP_013557

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011434 mRNA. Translation: BAB27619.1.
AK048904 mRNA. Translation: BAC33486.1.
BC021526 mRNA. Translation: AAH21526.1.
CCDSiCCDS23248.1. [Q8VDL4-1]
RefSeqiNP_082397.1. NM_028121.2.
UniGeneiMm.22456.

Genome annotation databases

GeneIDi72141.
KEGGimmu:72141.
UCSCiuc009pxo.1. mouse. [Q8VDL4-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK011434 mRNA. Translation: BAB27619.1.
AK048904 mRNA. Translation: BAC33486.1.
BC021526 mRNA. Translation: AAH21526.1.
CCDSiCCDS23248.1. [Q8VDL4-1]
RefSeqiNP_082397.1. NM_028121.2.
UniGeneiMm.22456.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5CCFX-ray2.10A51-496[»]
5CK7X-ray2.99A51-496[»]
ProteinModelPortaliQ8VDL4.
SMRiQ8VDL4. Positions 109-488.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VDL4. 1 interaction.
MINTiMINT-4126462.
STRINGi10090.ENSMUSP00000026266.

PTM databases

PhosphoSiteiQ8VDL4.
SwissPalmiQ8VDL4.

Proteomic databases

EPDiQ8VDL4.
MaxQBiQ8VDL4.
PaxDbiQ8VDL4.
PRIDEiQ8VDL4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi72141.
KEGGimmu:72141.
UCSCiuc009pxo.1. mouse. [Q8VDL4-2]

Organism-specific databases

CTDi83440.
MGIiMGI:1919391. Adpgk.

Phylogenomic databases

eggNOGiKOG4184. Eukaryota.
ENOG410Z7J9. LUCA.
HOGENOMiHOG000233731.
HOVERGENiHBG050461.
InParanoidiQ8VDL4.
KOiK08074.
OrthoDBiEOG7V1FRC.
PhylomeDBiQ8VDL4.
TreeFamiTF313401.

Enzyme and pathway databases

UniPathwayiUPA00109.
BRENDAi2.7.1.147. 3474.
SABIO-RKQ8VDL4.

Miscellaneous databases

NextBioi335548.
PROiQ8VDL4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VDL4.
CleanExiMM_ADPGK.

Family and domain databases

Gene3Di3.40.1190.20. 1 hit.
InterProiIPR007666. ADP_PFK/GK.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR21208. PTHR21208. 1 hit.
PfamiPF04587. ADP_PFK_GK. 1 hit.
[Graphical view]
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS51255. ADPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Cerebellum and Vagina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  3. "Cloning and biochemical characterization of a novel mouse ADP-dependent glucokinase."
    Ronimus R.S., Morgan H.W.
    Biochem. Biophys. Res. Commun. 315:652-658(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiADPGK_MOUSE
AccessioniPrimary (citable) accession number: Q8VDL4
Secondary accession number(s): Q8C7Y9, Q9D0H2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: April 26, 2005
Last modified: March 16, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.