Reviewed, UniProtKB/Swiss-Prot Q8VDL4 (ADPGK_MOUSE)

Names and origin

Protein names

Recommended name:
    ADP-dependent glucokinase
      Short name=ADP-GK
      Short name=ADPGK
    EC=2.7.1.147

Gene names

Name:

Adpgk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency.
Catalytic activity	ADP + D-glucose = AMP + D-glucose 6-phosphate.
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Carbohydrate degradation; glycolysis.
Subunit structure	Monomer. Ref.3
Subcellular location	Secreted Potential.
Sequence similarities	Belongs to the ADP-dependent glucokinase family. Contains 1 ADPK (ADP-dependent kinase) domain.
Biophysicochemical properties	Kinetic parameters: KM=96 µM for D-glucose (at 37 degrees Celsius) KM=0.28 mM for ADP (at 37 degrees Celsius) KM=20 mM for ADP (at 37 degrees Celsius) Vmax=160 µmol/min/mg enzyme with glucose and ADP as substrates (at 37 degrees Celsius) pH dependence: Optimum pH is 5.75-6.5 and 8.75-9.0.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	Signal
Ligand	Magnesium Metal-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ADP-specific glucokinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: Q8VDL4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q8VDL4-2)

The sequence of this isoform differs from the canonical sequence as follows:
     154-159: HYVGGN → VCPTHQ
     160-496: Missing.

Isoform 3 (identifier: Q8VDL4-3)

The sequence of this isoform differs from the canonical sequence as follows:
     313-313: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 496	474	ADP-dependent glucokinase		PRO_0000184777
Regions
	Domain	52 – 496	445	ADPK
Sites
	Active site	481	1	Proton acceptor By similarity
	Metal binding	297	1	Magnesium By similarity
	Metal binding	328	1	Magnesium By similarity
	Metal binding	481	1	Magnesium By similarity
Natural variations
	Alternative sequence	154 – 159	6	HYVGGN → VCPTHQ in isoform 2.		VSP_013555
	Alternative sequence	160 – 496	337	Missing in isoform 2.		VSP_013556
	Alternative sequence	313	1	Missing in isoform 3.		VSP_013557
Experimental info
	Sequence conflict	318	1	A → T in BAB27619. Ref.1
	Sequence conflict	341	1	S → A in BAB27619. Ref.1
	Sequence conflict	373	1	A → S in AAH21526. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 26, 2005. Version 2. Checksum: CAFCB41D50131A54	FASTA	496	53,902
	10 20 30 40 50 60 MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP LSPESRLAAA 70 80 90 100 110 120 WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP GSGKDHAILH SRSDLEEAFL 130 140 150 160 170 180 YFMGKGAAAE RFFSDKETFH DIAQAASEFP GAQHYVGGNA ALIGQRFAAN TDLKVLLCGP 190 200 210 220 230 240 IGPKLHELLD DNVFVPPESL QEEDEFHLIL EYLAGEEWGP FKAPHANRFI FSHDLSNGAM 250 260 270 280 290 300 NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTAISDIPT GIPVHLELAS 310 320 330 340 350 360 MTNRELMSSI VHQQVFPAVA SLGLNEQELL FLSQSASGPH SSLSSWDGVP DVGMVSDILF 370 380 390 400 410 420 WILKEHGRSE NRASDLTRIH FHTLVYHILA TVDGHWANQL AAVAAGARVA GTQACATETI 430 440 450 460 470 480 DTNRVSLRAP QEFTTSHLES GSRIVLNPDK PVVEWHREGI TFHFTPVLVC KDPVRTVGLG 490 DAISAEGLFY SEARPD
	Isoform 2. Checksum: 0910744000BDE52B	FASTA	159	16,864
	10 20 30 40 50 60 MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP LSPESRLAAA 70 80 90 100 110 120 WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP GSGKDHAILH SRSDLEEAFL 130 140 150 YFMGKGAAAE RFFSDKETFH DIAQAASEFP GAQVCPTHQ
	Isoform 3. Checksum: B1341DD422E7076D	FASTA	495	53,774
	10 20 30 40 50 60 MALWRGSACA GFLALAVGCV FLLEPELPGT ALRSLWSSLR LGPAPVPVGP LSPESRLAAA 70 80 90 100 110 120 WDALIAQPAR RWRRVAVGVN ACVDVVISGV KLLQALGLSP GSGKDHAILH SRSDLEEAFL 130 140 150 160 170 180 YFMGKGAAAE RFFSDKETFH DIAQAASEFP GAQHYVGGNA ALIGQRFAAN TDLKVLLCGP 190 200 210 220 230 240 IGPKLHELLD DNVFVPPESL QEEDEFHLIL EYLAGEEWGP FKAPHANRFI FSHDLSNGAM 250 260 270 280 290 300 NMLEVFVSSL EEFQPDLVVL SGLHMMEGQS KELQRKRLLE VVTAISDIPT GIPVHLELAS 310 320 330 340 350 360 MTNRELMSSI VHQVFPAVAS LGLNEQELLF LSQSASGPHS SLSSWDGVPD VGMVSDILFW 370 380 390 400 410 420 ILKEHGRSEN RASDLTRIHF HTLVYHILAT VDGHWANQLA AVAAGARVAG TQACATETID 430 440 450 460 470 480 TNRVSLRAPQ EFTTSHLESG SRIVLNPDKP VVEWHREGIT FHFTPVLVCK DPVRTVGLGD 490 AISAEGLFYS EARPD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6J. Tissue: Cerebellum and Vagina.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3). Strain: FVB/N. Tissue: Mammary tumor.
[3]	"Cloning and biochemical characterization of a novel mouse ADP-dependent glucokinase." Ronimus R.S., Morgan H.W. Biochem. Biophys. Res. Commun. 315:652-658(2004) [PubMed: 14975750] [Abstract] Cited for: CHARACTERIZATION, SUBUNIT, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK011434 mRNA. Translation: BAB27619.1. AK048904 mRNA. Translation: BAC33486.1. BC021526 mRNA. Translation: AAH21526.1.
IPI	IPI00132529. IPI00556819. IPI00556820.
RefSeq	NP_082397.1.
UniGene	Mm.22456
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VDL4.
Proteomic databases
PRIDE	Q8VDL4.
Genome annotation databases
Ensembl	ENSMUST00000026266; ENSMUSP00000026266; ENSMUSG00000025236; Mus musculus. [Genome view]
GeneID	72141.
KEGG	mmu:72141.
UCSC	uc009pxo.1. mouse.
Organism-specific databases
CTD	72141.
MGI	MGI:1919391. Adpgk.
Phylogenomic databases
HOGENOM	HBG444580.
HOVERGEN	Q8VDL4.
InParanoid	Q8VDL4.
Enzyme and pathway databases
BRENDA	2.7.1.147. 244.
Gene expression databases
ArrayExpress	Q8VDL4.
Bgee	Q8VDL4.
CleanEx	MM_ADPGK.
Genevestigator	Q8VDL4.
GermOnline	ENSMUSG00000025236. Mus musculus.
Family and domain databases
InterPro	IPR007666. ADP_PFK/GK. [Graphical view]
PANTHER	PTHR21208. ADP_PFK/GK. 1 hit.
Pfam	PF04587. ADP_PFK_GK. 1 hit. [Graphical view]
PROSITE	PS51255. ADPK. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	335548.
SOURCE	Search...

Entry information

Entry name

ADPGK_MOUSE

Accession

Primary (citable) accession number: Q8VDL4
Secondary accession number(s): Q8C7Y9, Q9D0H2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	April 26, 2005
Last modified:	December 15, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)