Q8VDG6 (M3KL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 102.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Mitogen-activated protein kinase kinase kinase MLK4 EC=2.7.11.25 Alternative name(s): Mixed lineage kinase 4 | ||||
| Gene names |
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| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 1002 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways By similarity. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. UniProtKB P80192 |
| Cofactor | Magnesium By similarity. UniProtKB P80192 |
| Enzyme regulation | Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity. UniProtKB P80192 |
| Subunit structure | Homodimer By similarity. Interacts with TLR4 By similarity. UniProtKB P80192 |
| Post-translational modification | Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation By similarity. UniProtKB P80192 |
| Sequence similarities | Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. UniProtKB P80192 Contains 1 protein kinase domain. Contains 1 SH3 domain. |
| Sequence caution | The sequence BAD90469.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAD90469.1 differs from that shown. Reason: Frameshift at position 724. |
Ontologies
| Keywords | |
|---|---|
| Domain | Repeat SH3 domain |
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
| Technical term | Complete proteome Reference proteome |
| Gene Ontology (GO) | |
| Biological_process | MAPK cascade Inferred from electronic annotation. Source: GOC activation of MAPKK activityInferred from electronic annotation. Source: GOC |
| Molecular_function | ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activityInferred from electronic annotation. Source: EC protein tyrosine kinase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1002 | 1002 | Mitogen-activated protein kinase kinase kinase MLK4 | PRO_0000302761 | |||||
Regions | |||||||||
| Domain | 24 – 88 | 65 | SH3 | ||||||
| Domain | 110 – 390 | 281 | Protein kinase | ||||||
| Nucleotide binding | 116 – 124 | 9 | ATP By similarity UniProtKB P28523 | ||||||
| Region | 409 – 430 | 22 | Leucine-zipper 1 | ||||||
| Region | 444 – 466 | 23 | Leucine-zipper 2 | ||||||
| Compositional bias | 85 – 102 | 18 | Pro-rich | ||||||
Sites | |||||||||
| Active site | 247 | 1 | Proton acceptor By similarity UniProtKB P28523 | ||||||
| Binding site | 137 | 1 | ATP By similarity UniProtKB P28523 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 283 | 1 | Phosphothreonine; by autocatalysis By similarity UniProtKB P80192 | ||||||
| Modified residue | 287 | 1 | Phosphoserine; by autocatalysis and MAP4K1 By similarity UniProtKB P80192 | ||||||
| Modified residue | 512 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 576 | 1 | Phosphothreonine By similarity | ||||||
| Modified residue | 598 | 1 | Phosphoserine By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 411 | 1 | Missing in AAH21891. Ref.2 | ||||||
Sequences
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References
| [1] | "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryonic intestine. |
| [2] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AK220424 mRNA. Translation: BAD90469.1. Sequence problems. BC021891 mRNA. Translation: AAH21891.1. BC046448 mRNA. Translation: AAH46448.1. |
| IPI | IPI00123268. |
| RefSeq | NP_663583.2. NM_145608.2. |
| UniGene | Mm.216458. |
3D structure databases | |
| ProteinModelPortal | Q8VDG6. |
| SMR | Q8VDG6. Positions 28-85, 103-489. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | Q8VDG6. |
Proteomic databases | |
| PaxDb | Q8VDG6. |
| PRIDE | Q8VDG6. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000034316; ENSMUSP00000034316; ENSMUSG00000031853. |
| GeneID | 234878. |
| KEGG | mmu:234878. |
| UCSC | uc009nyq.1. mouse. |
Organism-specific databases | |
| MGI | MGI:2385307. BC021891. |
| Rouge | Search... |
Phylogenomic databases | |
| eggNOG | COG0515. |
| GeneTree | ENSGT00700000104091. |
| HOGENOM | HOG000060081. |
| HOVERGEN | HBG067662. |
| InParanoid | Q8VDG6. |
| OMA | ESALCEC. |
| OrthoDB | EOG44F68G. |
Gene expression databases | |
| Bgee | Q8VDG6. |
| CleanEx | MM_BC021891. |
| Genevestigator | Q8VDG6. |
Family and domain databases | |
| InterPro | IPR011009. Kinase-like_dom. IPR016231. MAPKKK9/10/11. IPR015785. MAPKKK9/10/11-like. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. IPR001452. SH3_domain. IPR020635. Tyr_kinase_cat_dom. [Graphical view] |
| PANTHER | PTHR23257:SF87. PTHR23257:SF87. 1 hit. |
| Pfam | PF07714. Pkinase_Tyr. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view] |
| PIRSF | PIRSF000556. MAPKKK9_11. 1 hit. |
| PRINTS | PR00452. SH3DOMAIN. PR00109. TYRKINASE. |
| SMART | SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view] |
| SUPFAM | SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit. |
| PROSITE | PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50002. SH3. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 382421. |
| SOURCE | Search... |
Entry information
| Entry name | M3KL4_MOUSE | ||||||||
| Accession | Primary (citable) accession number: Q8VDG6 Secondary accession number(s): Q5DTU6, Q811F5 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| Human and mouse protein kinases Human and mouse protein kinases: classification and index |
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with
