Mitogen-activated protein kinase kinase kinase MLK4

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Q8VDG6 (M3KL4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitogen-activated protein kinase kinase kinase MLK4
EC=2.7.11.25

Alternative name(s):
Mixed lineage kinase 4

Gene names

Name:	Mlk4
Synonyms:	Kiaa1804

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1002 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Negative regulator of TLR4 signaling. Does not activate JNK1/MAPK8 pathway, p38/MAPK14, nor ERK2/MAPK1 pathways By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB P80192
Cofactor	Magnesium By similarity. UniProtKB P80192
Enzyme regulation	Homodimerization via the leucine zipper domains is required for autophosphorylation and subsequent activation By similarity. UniProtKB P80192
Subunit structure	Homodimer By similarity. Interacts with TLR4 By similarity. UniProtKB P80192
Post-translational modification	Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation By similarity. UniProtKB P80192
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. UniProtKB P80192 Contains 1 protein kinase domain. Contains 1 SH3 domain.
Sequence caution	The sequence BAD90469.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. The sequence BAD90469.1 differs from that shown. Reason: Frameshift at position 724.

Ontologies

Keywords
Domain	Repeat SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	MAPK cascade Inferred from electronic annotation. Source: GOC activation of MAPKK activity Inferred from electronic annotation. Source: GOC
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1002

1002

Mitogen-activated protein kinase kinase kinase MLK4

PRO_0000302761

Regions

Domain

24 – 88

SH3

Domain

110 – 390

281

Protein kinase

Nucleotide binding

116 – 124

ATP By similarity UniProtKB P28523

Region

409 – 430

Leucine-zipper 1

Region

444 – 466

Leucine-zipper 2

Compositional bias

85 – 102

Pro-rich

Sites

Active site

247

Proton acceptor By similarity UniProtKB P28523

Binding site

137

ATP By similarity UniProtKB P28523

Amino acid modifications

Modified residue

283

Phosphothreonine; by autocatalysis By similarity UniProtKB P80192

Modified residue

287

Phosphoserine; by autocatalysis and MAP4K1 By similarity UniProtKB P80192

Modified residue

512

Phosphoserine By similarity

Modified residue

576

Phosphothreonine By similarity

Modified residue

598

Phosphoserine By similarity

Experimental info

Sequence conflict

411

Missing in AAH21891. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

Q8VDG6 [UniParc].

Last modified September 11, 2007. Version 2.
Checksum: C4E706833A578C78
Show »

FASTA

1,002

110,112

        10         20         30         40         50         60 
MALPVAEGTA DTPLSPARDD SGSTSSGMWA ALYDYEARGE DELSLRRGQL VEVLSQDAAV 

        70         80         90        100        110        120 
SGDEGWWAGQ VQRRLGIFPA SYVAPCGPVP PPAPPPPRPC SPVHVDFERL ELKELIGAGG 

       130        140        150        160        170        180 
FGQVYRATWQ GQEVAVKAAR RDPEQDAAAA AESVRREARL FAMLRHPNII QLRGVCLRQP 

       190        200        210        220        230        240 
HLCLVLEFAR GGALNRALAA AASDPRAPGP RRARRIPPQV LVNWAVQIAR GMLYLHEEAV 

       250        260        270        280        290        300 
VPILHRDLKS SNILLLEKIE HDDICNKTLK ITDFGLAREW HRTTRMSAAG TYAWMAPEVI 

       310        320        330        340        350        360 
RSSLFSKGSD IWSYGVLLWE LLTGEVPYRG IDGLAVAYGV AVNKLTLPIP STCPEPFAKL 

       370        380        390        400        410        420 
MKECWEQDPH IRPSFALILQ QLTAIEEAVL TNMPQESFHS MQEDWKLEIQ QMFSELRTKE 

       430        440        450        460        470        480 
KELRSREEEL SRAALQQKSQ ELLLRRREQQ LAEREIDVLE RELNVLIFQL SQEAPHVKKR 

       490        500        510        520        530        540 
KGRFRRGRLR LKDGHRISLP SDFQHKITVQ ASPTLDKRRS SDSGLCSPPG SPLMLPRLRA 

       550        560        570        580        590        600 
IQLTSDENNK TRGRNMVFRQ EDFEDVKRSF KKKGCTWGPS SVQTKERPEG RERVRPLSDG 

       610        620        630        640        650        660 
NSPWSSLLIK SQKTTPLASL FVDQPGSCEE QKLVPEGLEH RKPKQTKFPG QAHVGLPLCK 

       670        680        690        700        710        720 
DSQREDSSEA ESREEGSPKG SPVNNVGAPM LRKKTESALC ECGMLLASMA LGLDVRKLHG 

       730        740        750        760        770        780 
AQAPAKPSPK MEKKEEGALQ PASRCQSSPS SLLRQPSAGR APSGGSTLLL PSAPSHSSKS 

       790        800        810        820        830        840 
SLSMKCLLQA GKEESSLGNA RDLCGPTTLT PDPGSAAPES GCELIPGLRP KTDYGVLRSM 

       850        860        870        880        890        900 
PHAILEQTGE RLPGCAIVGD KGCHHMQMGS EETPLWLQSA PEDSGLPHSP SPGPQRDLAS 

       910        920        930        940        950        960 
QASLVKPEGV LGECQACPAL PQRPHTASVR TTSPPTWVCD KDHQVPALAC LLGAQERSRC 

       970        980        990       1000 
QTPSLLDASI EGQKKDCAMP LCRVKSVMCQ PSIYALEKDF LT

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References

[1]	"Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries." Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H. Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryonic intestine.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK220424 mRNA. Translation: BAD90469.1. Sequence problems. BC021891 mRNA. Translation: AAH21891.1. BC046448 mRNA. Translation: AAH46448.1.
IPI	IPI00123268.
RefSeq	NP_663583.2. NM_145608.2.
UniGene	Mm.216458.
3D structure databases
ProteinModelPortal	Q8VDG6.
SMR	Q8VDG6. Positions 28-85, 103-489.
ModBase	Search...
PTM databases
PhosphoSite	Q8VDG6.
Proteomic databases
PaxDb	Q8VDG6.
PRIDE	Q8VDG6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034316; ENSMUSP00000034316; ENSMUSG00000031853.
GeneID	234878.
KEGG	mmu:234878.
UCSC	uc009nyq.1. mouse.
Organism-specific databases
MGI	MGI:2385307. BC021891.
Rouge	Search...
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104091.
HOGENOM	HOG000060081.
HOVERGEN	HBG067662.
InParanoid	Q8VDG6.
OMA	ESALCEC.
OrthoDB	EOG44F68G.
Gene expression databases
Bgee	Q8VDG6.
CleanEx	MM_BC021891.
Genevestigator	Q8VDG6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR016231. MAPKKK9/10/11. IPR015785. MAPKKK9/10/11-like. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR008271. Ser/Thr_kinase_AS. IPR001452. SH3_domain. IPR020635. Tyr_kinase_cat_dom. [Graphical view]
PANTHER	PTHR23257:SF87. PTHR23257:SF87. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PIRSF	PIRSF000556. MAPKKK9_11. 1 hit.
PRINTS	PR00452. SH3DOMAIN. PR00109. TYRKINASE.
SMART	SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF50044. SH3. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	382421.
SOURCE	Search...

Entry information

Entry name

M3KL4_MOUSE

Accession

Primary (citable) accession number: Q8VDG6
Secondary accession number(s): Q5DTU6, Q811F5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 11, 2007
Last sequence update:	September 11, 2007
Last modified:	May 29, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents