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Q8VDG5 (PPCS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphopantothenate--cysteine ligase

EC=6.3.2.5
Alternative name(s):
Phosphopantothenoylcysteine synthetase
Short name=PPC synthetase
Gene names
Name:Ppcs
Synonyms:Coab
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine By similarity.

Catalytic activity

CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Pathway

Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 2/5.

Subunit structure

Homodimer By similarity.

Miscellaneous

The mammalian enzyme has a preference for ATP over CTP, in contrast to the E.coli ortholog By similarity.

Sequence similarities

Belongs to the PPC synthetase family.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   Molecular functionLigase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcoenzyme A biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionphosphopantothenate--cysteine ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VDG5-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VDG5-2)

The sequence of this isoform differs from the canonical sequence as follows:
     2-173: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 311310Phosphopantothenate--cysteine ligase
PRO_0000182041

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Natural variations

Alternative sequence2 – 173172Missing in isoform 2.
VSP_010244

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 082E03CB2E3A11E6

FASTA31133,794
        10         20         30         40         50         60 
MAEMDLVAEL PRPAGAARWA EVMARFAARL GEQGRRVVLI TSGGTKVPLE ARAVRFLDNF 

        70         80         90        100        110        120 
SSGRRGAASA EVFLAAGYGV LFLYRARSAF PYAHRFPPQA WLSALRPSGP AQSGKLSLEA 

       130        140        150        160        170        180 
EENALPGFAA ALQSYQEAAA AGTFLAVEFT TLADYLHLLQ AAALALSPLG SSAMFYLAAA 

       190        200        210        220        230        240 
VSDFYIPVSE MPEHKIHSSG GPLQITMKMV PKMLSPLVKD WAPKAFVVSF KLETDPDIII 

       250        260        270        280        290        300 
SRARNALEVY QHQVVVANIL ESIKSFVIIV TKDSETELLL SEEEVAKGLV IEEKIVDDLR 

       310 
SRHTAFICDK N 

« Hide

Isoform 2 [UniParc].

Checksum: EC8707370992774E
Show »

FASTA13915,675

References

[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J.
Tissue: Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: Czech II.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK006290 mRNA. Translation: BAB24509.1.
AK159952 mRNA. Translation: BAE35508.1.
BC021894 mRNA. Translation: AAH21894.1.
CCDSCCDS18581.1. [Q8VDG5-1]
RefSeqNP_080770.2. NM_026494.3. [Q8VDG5-1]
XP_006502700.1. XM_006502637.1.
XP_006502701.1. XM_006502638.1.
UniGeneMm.27245.

3D structure databases

ProteinModelPortalQ8VDG5.
SMRQ8VDG5. Positions 7-307.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8VDG5. 1 interaction.
MINTMINT-4108416.

PTM databases

PhosphoSiteQ8VDG5.

2D gel databases

REPRODUCTION-2DPAGEIPI00135484.

Proteomic databases

MaxQBQ8VDG5.
PaxDbQ8VDG5.
PRIDEQ8VDG5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030385; ENSMUSP00000030385; ENSMUSG00000028636. [Q8VDG5-1]
ENSMUST00000106316; ENSMUSP00000101923; ENSMUSG00000028636.
GeneID106564.
KEGGmmu:106564.
UCSCuc008umm.1. mouse. [Q8VDG5-1]

Organism-specific databases

CTD79717.
MGIMGI:1915237. Ppcs.

Phylogenomic databases

eggNOGCOG0452.
GeneTreeENSGT00390000015263.
HOGENOMHOG000194726.
HOVERGENHBG049438.
InParanoidQ8VDG5.
KOK01922.
OMARSAFPYA.
OrthoDBEOG7TTQ82.
PhylomeDBQ8VDG5.
TreeFamTF105615.

Enzyme and pathway databases

UniPathwayUPA00241; UER00353.

Gene expression databases

BgeeQ8VDG5.
CleanExMM_PPCS.
GenevestigatorQ8VDG5.

Family and domain databases

Gene3D3.40.50.10300. 1 hit.
InterProIPR007085. DNA/pantothenate-metab_flavo_C.
[Graphical view]
PfamPF04127. DFP. 2 hits.
[Graphical view]
SUPFAMSSF102645. SSF102645. 1 hit.
ProtoNetSearch...

Other

NextBio358258.
PROQ8VDG5.
SOURCESearch...

Entry information

Entry namePPCS_MOUSE
AccessionPrimary (citable) accession number: Q8VDG5
Secondary accession number(s): Q3TVW0, Q9D376, Q9DA06
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot