Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphopantothenate--cysteine ligase

Gene

Ppcs

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the first step in the biosynthesis of coenzyme A from vitamin B5, where cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine.By similarity

Catalytic activityi

CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine.

Pathwayi

GO - Molecular functioni

  1. phosphopantothenate--cysteine ligase activity Source: MGI

GO - Biological processi

  1. coenzyme A biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Enzyme and pathway databases

ReactomeiREACT_216415. Coenzyme A biosynthesis.
UniPathwayiUPA00241; UER00353.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphopantothenate--cysteine ligase (EC:6.3.2.5)
Alternative name(s):
Phosphopantothenoylcysteine synthetase
Short name:
PPC synthetase
Gene namesi
Name:Ppcs
Synonyms:Coab
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1915237. Ppcs.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 311310Phosphopantothenate--cysteine ligasePRO_0000182041Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8VDG5.
PaxDbiQ8VDG5.
PRIDEiQ8VDG5.

2D gel databases

REPRODUCTION-2DPAGEIPI00135484.

PTM databases

PhosphoSiteiQ8VDG5.

Expressioni

Gene expression databases

BgeeiQ8VDG5.
CleanExiMM_PPCS.
GenevestigatoriQ8VDG5.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ8VDG5. 1 interaction.
MINTiMINT-4108416.

Structurei

3D structure databases

ProteinModelPortaliQ8VDG5.
SMRiQ8VDG5. Positions 7-307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPC synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0452.
GeneTreeiENSGT00390000015263.
HOGENOMiHOG000194726.
HOVERGENiHBG049438.
InParanoidiQ8VDG5.
KOiK01922.
OMAiRSAFPYA.
OrthoDBiEOG7TTQ82.
PhylomeDBiQ8VDG5.
TreeFamiTF105615.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
InterProiIPR007085. DNA/pantothenate-metab_flavo_C.
[Graphical view]
PfamiPF04127. DFP. 2 hits.
[Graphical view]
SUPFAMiSSF102645. SSF102645. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: Q8VDG5-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAEMDLVAEL PRPAGAARWA EVMARFAARL GEQGRRVVLI TSGGTKVPLE
60 70 80 90 100
ARAVRFLDNF SSGRRGAASA EVFLAAGYGV LFLYRARSAF PYAHRFPPQA
110 120 130 140 150
WLSALRPSGP AQSGKLSLEA EENALPGFAA ALQSYQEAAA AGTFLAVEFT
160 170 180 190 200
TLADYLHLLQ AAALALSPLG SSAMFYLAAA VSDFYIPVSE MPEHKIHSSG
210 220 230 240 250
GPLQITMKMV PKMLSPLVKD WAPKAFVVSF KLETDPDIII SRARNALEVY
260 270 280 290 300
QHQVVVANIL ESIKSFVIIV TKDSETELLL SEEEVAKGLV IEEKIVDDLR
310
SRHTAFICDK N
Length:311
Mass (Da):33,794
Last modified:March 1, 2002 - v1
Checksum:i082E03CB2E3A11E6
GO
Isoform 2 (identifier: Q8VDG5-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-173: Missing.

Note: No experimental confirmation available.

Show »
Length:138
Mass (Da):15,544
Checksum:i923616BF66AB774E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 173173Missing in isoform 2. 1 PublicationVSP_010244Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006290 mRNA. Translation: BAB24509.1.
AK159952 mRNA. Translation: BAE35508.1.
BC021894 mRNA. Translation: AAH21894.1.
CCDSiCCDS18581.1. [Q8VDG5-1]
RefSeqiNP_080770.2. NM_026494.3. [Q8VDG5-1]
XP_006502700.1. XM_006502637.1. [Q8VDG5-2]
XP_006502701.1. XM_006502638.1. [Q8VDG5-2]
UniGeneiMm.27245.

Genome annotation databases

EnsembliENSMUST00000030385; ENSMUSP00000030385; ENSMUSG00000028636. [Q8VDG5-1]
ENSMUST00000106316; ENSMUSP00000101923; ENSMUSG00000028636. [Q8VDG5-2]
GeneIDi106564.
KEGGimmu:106564.
UCSCiuc008umm.1. mouse. [Q8VDG5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK006290 mRNA. Translation: BAB24509.1.
AK159952 mRNA. Translation: BAE35508.1.
BC021894 mRNA. Translation: AAH21894.1.
CCDSiCCDS18581.1. [Q8VDG5-1]
RefSeqiNP_080770.2. NM_026494.3. [Q8VDG5-1]
XP_006502700.1. XM_006502637.1. [Q8VDG5-2]
XP_006502701.1. XM_006502638.1. [Q8VDG5-2]
UniGeneiMm.27245.

3D structure databases

ProteinModelPortaliQ8VDG5.
SMRiQ8VDG5. Positions 7-307.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VDG5. 1 interaction.
MINTiMINT-4108416.

PTM databases

PhosphoSiteiQ8VDG5.

2D gel databases

REPRODUCTION-2DPAGEIPI00135484.

Proteomic databases

MaxQBiQ8VDG5.
PaxDbiQ8VDG5.
PRIDEiQ8VDG5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030385; ENSMUSP00000030385; ENSMUSG00000028636. [Q8VDG5-1]
ENSMUST00000106316; ENSMUSP00000101923; ENSMUSG00000028636. [Q8VDG5-2]
GeneIDi106564.
KEGGimmu:106564.
UCSCiuc008umm.1. mouse. [Q8VDG5-1]

Organism-specific databases

CTDi79717.
MGIiMGI:1915237. Ppcs.

Phylogenomic databases

eggNOGiCOG0452.
GeneTreeiENSGT00390000015263.
HOGENOMiHOG000194726.
HOVERGENiHBG049438.
InParanoidiQ8VDG5.
KOiK01922.
OMAiRSAFPYA.
OrthoDBiEOG7TTQ82.
PhylomeDBiQ8VDG5.
TreeFamiTF105615.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00353.
ReactomeiREACT_216415. Coenzyme A biosynthesis.

Miscellaneous databases

NextBioi358258.
PROiQ8VDG5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VDG5.
CleanExiMM_PPCS.
GenevestigatoriQ8VDG5.

Family and domain databases

Gene3Di3.40.50.10300. 1 hit.
InterProiIPR007085. DNA/pantothenate-metab_flavo_C.
[Graphical view]
PfamiPF04127. DFP. 2 hits.
[Graphical view]
SUPFAMiSSF102645. SSF102645. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: Czech II.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiPPCS_MOUSE
AccessioniPrimary (citable) accession number: Q8VDG5
Secondary accession number(s): Q3TVW0, Q9D376, Q9DA06
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: March 1, 2002
Last modified: February 4, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The mammalian enzyme has a preference for ATP over CTP, in contrast to the E.coli ortholog.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.