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Q8VDC0

- SYLM_MOUSE

UniProt

Q8VDC0 - SYLM_MOUSE

Protein

Probable leucine--tRNA ligase, mitochondrial

Gene

Lars2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei641 – 6411ATPBy similarity

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. leucine-tRNA ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: InterPro

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
    Alternative name(s):
    Leucyl-tRNA synthetase
    Short name:
    LeuRS
    Gene namesi
    Name:Lars2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:2142973. Lars2.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell
    2. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 902Probable leucine--tRNA ligase, mitochondrialPRO_0000035807
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei67 – 671N6-acetyllysine1 Publication
    Modified residuei235 – 2351N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8VDC0.
    PaxDbiQ8VDC0.
    PRIDEiQ8VDC0.

    PTM databases

    PhosphoSiteiQ8VDC0.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8VDC0.
    BgeeiQ8VDC0.
    CleanExiMM_LARS2.
    GenevestigatoriQ8VDC0.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8VDC0. 1 interaction.
    MINTiMINT-4136305.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VDC0.
    SMRiQ8VDC0. Positions 52-891.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi91 – 10111"HIGH" regionAdd
    BLAST
    Motifi638 – 6425"KMSKS" region

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0495.
    GeneTreeiENSGT00390000015114.
    HOGENOMiHOG000200747.
    HOVERGENiHBG017877.
    InParanoidiA6H6S4.
    KOiK01869.
    OMAiHISHELW.
    OrthoDBiEOG754HNN.
    PhylomeDBiQ8VDC0.
    TreeFamiTF105662.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VDC0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASTCQRLSF YVSPLKRQLV SRPPVILWER LIPGCSRSIY SATGKWTKEY    50
    TLQTRKDVEK WWHQQIKEQA SRVSEEDKLK PKFYLLSMFP YPSGKLHMGH 100
    VRVYTLSDTI ARFQKMRGMQ VINPMGWDAF GLPAENAAIE RNLHPESWTQ 150
    SNIKHMRKQL DRLGLCFSWD REITTCLPDY YKWTQYLFIK LYEAGLAYQK 200
    EALVNWDPVD QTVLANEQVN EYGCSWRSGA KVEKKYLRQW FIKTTAYAKA 250
    MQDALADLPE WYGIKGMQAH WIGDCVGCHL DFTLKVDGED TGEKLTAYTA 300
    TPEAIYGISH VAISPSHGLL HGCSSVKKAL QKALVPGRDC LTPVMAVSML 350
    TLQEVPIVIM ANPDLEGSLD SKIGIPSTSS EDTRLAQALG LPYSEVIEAS 400
    PDGTERLSGS AEFTGMTRQD AFVALTRKAR GMRVGGHVTS NKLKDWLISR 450
    QRYWGTPIPI VHCPACGPVP VPLQDLPVIL PSIASLTGRG GSPLATALEW 500
    VNCSCPRCKG SAKRETDTMD TFVDSAWYYF RYTDPHNTQS PFGSALADFW 550
    MPVDLYIGGK EHAVMHLFYA RFLSHFCHDQ KMVKHREPFH KLLAQGLIKG 600
    QTFRLPSGQC LKKEDIDFTG PAPVCAKTKE KLEVTWEKMS KSKHNGVDPE 650
    EIVAQYGIDT IRLYILFAAP PEKDILWDVK TDALPGVLRW QQRLWSLTTR 700
    FIEARTSGTV PQPQLLNSKE KTKAQNLWEY KNAVIAQVTT HFTEDFALNS 750
    VVSQLMGLSS ALSQASQRVV LHSPEFEDAL CALLVMAAPL APHVTSELWA 800
    GLTLVPSKLC DHYAWDSGVM LQAWPTVDSQ FLQKPDMVQM AVLINNKACG 850
    KIPVPQHVAQ DQDKVHELVL QSELGMKLLQ GRSIKKAFLS PRTALINFLV 900
    QE 902
    Length:902
    Mass (Da):101,480
    Last modified:March 1, 2002 - v1
    Checksum:iF5ED21E130F3A5FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ428066 mRNA. Translation: CAD20988.1.
    BC080303 mRNA. Translation: AAH80303.1.
    BC138206 mRNA. Translation: AAI38207.1.
    BC145982 mRNA. Translation: AAI45983.1.
    CCDSiCCDS23659.1.
    RefSeqiNP_694808.1. NM_153168.2.
    UniGeneiMm.276076.

    Genome annotation databases

    EnsembliENSMUST00000038863; ENSMUSP00000036710; ENSMUSG00000035202.
    GeneIDi102436.
    KEGGimmu:102436.
    UCSCiuc009sgd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ428066 mRNA. Translation: CAD20988.1 .
    BC080303 mRNA. Translation: AAH80303.1 .
    BC138206 mRNA. Translation: AAI38207.1 .
    BC145982 mRNA. Translation: AAI45983.1 .
    CCDSi CCDS23659.1.
    RefSeqi NP_694808.1. NM_153168.2.
    UniGenei Mm.276076.

    3D structure databases

    ProteinModelPortali Q8VDC0.
    SMRi Q8VDC0. Positions 52-891.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VDC0. 1 interaction.
    MINTi MINT-4136305.

    PTM databases

    PhosphoSitei Q8VDC0.

    Proteomic databases

    MaxQBi Q8VDC0.
    PaxDbi Q8VDC0.
    PRIDEi Q8VDC0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000038863 ; ENSMUSP00000036710 ; ENSMUSG00000035202 .
    GeneIDi 102436.
    KEGGi mmu:102436.
    UCSCi uc009sgd.1. mouse.

    Organism-specific databases

    CTDi 23395.
    MGIi MGI:2142973. Lars2.

    Phylogenomic databases

    eggNOGi COG0495.
    GeneTreei ENSGT00390000015114.
    HOGENOMi HOG000200747.
    HOVERGENi HBG017877.
    InParanoidi A6H6S4.
    KOi K01869.
    OMAi HISHELW.
    OrthoDBi EOG754HNN.
    PhylomeDBi Q8VDC0.
    TreeFami TF105662.

    Miscellaneous databases

    NextBioi 355474.
    PROi Q8VDC0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VDC0.
    Bgeei Q8VDC0.
    CleanExi MM_LARS2.
    Genevestigatori Q8VDC0.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 3 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative human/murine sequence analysis of the common eliminated region 1 from human 3p21.3."
      Kiss H., Darai E., Kiss C., Kost-Alimova M., Klein G., Dumanski J.P., Imreh S.
      Mamm. Genome 13:646-655(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
      Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
      Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiSYLM_MOUSE
    AccessioniPrimary (citable) accession number: Q8VDC0
    Secondary accession number(s): A6H6S4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2004
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3