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Protein

Probable leucine--tRNA ligase, mitochondrial

Gene

Lars2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei641 – 6411ATPBy similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. leucine-tRNA ligase activity Source: GO_Central

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable leucine--tRNA ligase, mitochondrial (EC:6.1.1.4)
Alternative name(s):
Leucyl-tRNA synthetase
Short name:
LeuRS
Gene namesi
Name:Lars2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:2142973. Lars2.

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 902Probable leucine--tRNA ligase, mitochondrialPRO_0000035807
Transit peptidei1 – ?MitochondrionSequence Analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671N6-acetyllysine1 Publication
Modified residuei235 – 2351N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8VDC0.
PaxDbiQ8VDC0.
PRIDEiQ8VDC0.

PTM databases

PhosphoSiteiQ8VDC0.

Expressioni

Gene expression databases

BgeeiQ8VDC0.
CleanExiMM_LARS2.
ExpressionAtlasiQ8VDC0. baseline and differential.
GenevestigatoriQ8VDC0.

Interactioni

Protein-protein interaction databases

IntActiQ8VDC0. 1 interaction.
MINTiMINT-4136305.

Structurei

3D structure databases

ProteinModelPortaliQ8VDC0.
SMRiQ8VDC0. Positions 52-891.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi91 – 10111"HIGH" regionAdd
BLAST
Motifi638 – 6425"KMSKS" region

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000015114.
HOGENOMiHOG000200747.
HOVERGENiHBG017877.
InParanoidiQ8VDC0.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG754HNN.
PhylomeDBiQ8VDC0.
TreeFamiTF105662.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VDC0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTCQRLSF YVSPLKRQLV SRPPVILWER LIPGCSRSIY SATGKWTKEY
60 70 80 90 100
TLQTRKDVEK WWHQQIKEQA SRVSEEDKLK PKFYLLSMFP YPSGKLHMGH
110 120 130 140 150
VRVYTLSDTI ARFQKMRGMQ VINPMGWDAF GLPAENAAIE RNLHPESWTQ
160 170 180 190 200
SNIKHMRKQL DRLGLCFSWD REITTCLPDY YKWTQYLFIK LYEAGLAYQK
210 220 230 240 250
EALVNWDPVD QTVLANEQVN EYGCSWRSGA KVEKKYLRQW FIKTTAYAKA
260 270 280 290 300
MQDALADLPE WYGIKGMQAH WIGDCVGCHL DFTLKVDGED TGEKLTAYTA
310 320 330 340 350
TPEAIYGISH VAISPSHGLL HGCSSVKKAL QKALVPGRDC LTPVMAVSML
360 370 380 390 400
TLQEVPIVIM ANPDLEGSLD SKIGIPSTSS EDTRLAQALG LPYSEVIEAS
410 420 430 440 450
PDGTERLSGS AEFTGMTRQD AFVALTRKAR GMRVGGHVTS NKLKDWLISR
460 470 480 490 500
QRYWGTPIPI VHCPACGPVP VPLQDLPVIL PSIASLTGRG GSPLATALEW
510 520 530 540 550
VNCSCPRCKG SAKRETDTMD TFVDSAWYYF RYTDPHNTQS PFGSALADFW
560 570 580 590 600
MPVDLYIGGK EHAVMHLFYA RFLSHFCHDQ KMVKHREPFH KLLAQGLIKG
610 620 630 640 650
QTFRLPSGQC LKKEDIDFTG PAPVCAKTKE KLEVTWEKMS KSKHNGVDPE
660 670 680 690 700
EIVAQYGIDT IRLYILFAAP PEKDILWDVK TDALPGVLRW QQRLWSLTTR
710 720 730 740 750
FIEARTSGTV PQPQLLNSKE KTKAQNLWEY KNAVIAQVTT HFTEDFALNS
760 770 780 790 800
VVSQLMGLSS ALSQASQRVV LHSPEFEDAL CALLVMAAPL APHVTSELWA
810 820 830 840 850
GLTLVPSKLC DHYAWDSGVM LQAWPTVDSQ FLQKPDMVQM AVLINNKACG
860 870 880 890 900
KIPVPQHVAQ DQDKVHELVL QSELGMKLLQ GRSIKKAFLS PRTALINFLV

QE
Length:902
Mass (Da):101,480
Last modified:March 1, 2002 - v1
Checksum:iF5ED21E130F3A5FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ428066 mRNA. Translation: CAD20988.1.
BC080303 mRNA. Translation: AAH80303.1.
BC138206 mRNA. Translation: AAI38207.1.
BC145982 mRNA. Translation: AAI45983.1.
CCDSiCCDS23659.1.
RefSeqiNP_694808.1. NM_153168.2.
UniGeneiMm.276076.

Genome annotation databases

EnsembliENSMUST00000038863; ENSMUSP00000036710; ENSMUSG00000035202.
GeneIDi102436.
KEGGimmu:102436.
UCSCiuc009sgd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ428066 mRNA. Translation: CAD20988.1.
BC080303 mRNA. Translation: AAH80303.1.
BC138206 mRNA. Translation: AAI38207.1.
BC145982 mRNA. Translation: AAI45983.1.
CCDSiCCDS23659.1.
RefSeqiNP_694808.1. NM_153168.2.
UniGeneiMm.276076.

3D structure databases

ProteinModelPortaliQ8VDC0.
SMRiQ8VDC0. Positions 52-891.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VDC0. 1 interaction.
MINTiMINT-4136305.

PTM databases

PhosphoSiteiQ8VDC0.

Proteomic databases

MaxQBiQ8VDC0.
PaxDbiQ8VDC0.
PRIDEiQ8VDC0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000038863; ENSMUSP00000036710; ENSMUSG00000035202.
GeneIDi102436.
KEGGimmu:102436.
UCSCiuc009sgd.1. mouse.

Organism-specific databases

CTDi23395.
MGIiMGI:2142973. Lars2.

Phylogenomic databases

eggNOGiCOG0495.
GeneTreeiENSGT00390000015114.
HOGENOMiHOG000200747.
HOVERGENiHBG017877.
InParanoidiQ8VDC0.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG754HNN.
PhylomeDBiQ8VDC0.
TreeFamiTF105662.

Miscellaneous databases

NextBioi355474.
PROiQ8VDC0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VDC0.
CleanExiMM_LARS2.
ExpressionAtlasiQ8VDC0. baseline and differential.
GenevestigatoriQ8VDC0.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Comparative human/murine sequence analysis of the common eliminated region 1 from human 3p21.3."
    Kiss H., Darai E., Kiss C., Kost-Alimova M., Klein G., Dumanski J.P., Imreh S.
    Mamm. Genome 13:646-655(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSYLM_MOUSE
AccessioniPrimary (citable) accession number: Q8VDC0
Secondary accession number(s): A6H6S4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: March 1, 2002
Last modified: March 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.