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Q8VDB2

- ALG12_MOUSE

UniProt

Q8VDB2 - ALG12_MOUSE

Protein

Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase

Gene

Alg12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 2 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Adds the eighth mannose residue in an alpha-1,6 linkage onto the dolichol-PP-oligosaccharide precursor (dolichol-PP-Man7GlcNAc2) required for protein glycosylation.By similarity

    Catalytic activityi

    Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

    Pathwayi

    GO - Molecular functioni

    1. dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. dolichol-linked oligosaccharide biosynthetic process Source: MGI
    2. protein N-linked glycosylation Source: Ensembl

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT22. Glycosyltransferase Family 22.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferase (EC:2.4.1.260)
    Alternative name(s):
    Asparagine-linked glycosylation protein 12 homolog
    Dolichyl-P-Man:Man(7)GlcNAc(2)-PP-dolichyl-alpha-1,6-mannosyltransferase
    Mannosyltransferase ALG12 homolog
    Gene namesi
    Name:Alg12
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:2385025. Alg12.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 486486Dol-P-Man:Man(7)GlcNAc(2)-PP-Dol alpha-1,6-mannosyltransferasePRO_0000215782Add
    BLAST

    Proteomic databases

    PaxDbiQ8VDB2.
    PRIDEiQ8VDB2.

    PTM databases

    PhosphoSiteiQ8VDB2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ8VDB2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VDB2.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei94 – 11421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei123 – 14321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei213 – 23321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei265 – 28521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei291 – 31121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei313 – 33321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei344 – 36421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 22 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG256776.
    GeneTreeiENSGT00550000075005.
    HOGENOMiHOG000265945.
    HOVERGENiHBG050486.
    InParanoidiQ8VDB2.
    KOiK03847.
    OMAiFHLMFYS.
    OrthoDBiEOG7ZGX2X.
    TreeFamiTF314453.

    Family and domain databases

    InterProiIPR005599. GPI_mannosylTrfase.
    [Graphical view]
    PANTHERiPTHR22760. PTHR22760. 1 hit.
    PfamiPF03901. Glyco_transf_22. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VDB2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGKKSSGKR SWPLLGLLVT VATIHLVICP YTKVEESFNL QATHDLLYHQ    50
    LDIDKYDHHE FPGVVPRTFL GPLVIAAFSS PVVYVLSLLE VSKFYSQLIV 100
    RGVLGLGVIS GLWTLQKEVR QQFGATVAVM FCWISATQFH LMFYCTRTLP 150
    NVLALAVVLP ALTAWLQRRW ALFVWLSAFV IIGFRAELAM LLGIALLLTL 200
    YQRRLTVARV LRHAIPAGLL CLGLTVAVDS YFWRYLVWPE GVVLWYNTVL 250
    NKSSNWGTSP LLWYFYSALP RGLGCSLLFI PLGAVDRRTY ALALPSLGFV 300
    ALYSLLPHKE LRFIIYTFPV LNIMAARGCT YILNKKSWPY KVRAMLVTGH 350
    ILVNVAYTAT SLYVSHFNYP GGVAMQQLHE LVPPQTDVLL HIDVAAAQTG 400
    VSRFLQVNDD WRYDKSEDVG AAAMLNYTHI LMEAVPGHPA LYRDTHRVLA 450
    SIEGTTGISL NLMKLPPFDV NLQTKLVLLE RLLRPA 486
    Length:486
    Mass (Da):54,543
    Last modified:September 18, 2013 - v2
    Checksum:i011C1DA60919D0A3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti245 – 2451W → G in CAD22101. (PubMed:12200473)Curated
    Sequence conflicti476 – 48510LVLLERLLRP → AGTSRAAA in CAD22101. (PubMed:12200473)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ429133 mRNA. Translation: CAD22101.1.
    AK142322 mRNA. Translation: BAE25031.1.
    AC117700 Genomic DNA. No translation available.
    CCDSiCCDS49694.1.
    RefSeqiNP_001135829.1. NM_001142357.1.
    NP_663452.1. NM_145477.2.
    XP_006520905.1. XM_006520842.1.
    UniGeneiMm.41512.

    Genome annotation databases

    EnsembliENSMUST00000162183; ENSMUSP00000123935; ENSMUSG00000035845.
    GeneIDi223774.
    KEGGimmu:223774.
    UCSCiuc007xeo.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ429133 mRNA. Translation: CAD22101.1 .
    AK142322 mRNA. Translation: BAE25031.1 .
    AC117700 Genomic DNA. No translation available.
    CCDSi CCDS49694.1.
    RefSeqi NP_001135829.1. NM_001142357.1.
    NP_663452.1. NM_145477.2.
    XP_006520905.1. XM_006520842.1.
    UniGenei Mm.41512.

    3D structure databases

    ProteinModelPortali Q8VDB2.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT22. Glycosyltransferase Family 22.

    PTM databases

    PhosphoSitei Q8VDB2.

    Proteomic databases

    PaxDbi Q8VDB2.
    PRIDEi Q8VDB2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000162183 ; ENSMUSP00000123935 ; ENSMUSG00000035845 .
    GeneIDi 223774.
    KEGGi mmu:223774.
    UCSCi uc007xeo.2. mouse.

    Organism-specific databases

    CTDi 79087.
    MGIi MGI:2385025. Alg12.

    Phylogenomic databases

    eggNOGi NOG256776.
    GeneTreei ENSGT00550000075005.
    HOGENOMi HOG000265945.
    HOVERGENi HBG050486.
    InParanoidi Q8VDB2.
    KOi K03847.
    OMAi FHLMFYS.
    OrthoDBi EOG7ZGX2X.
    TreeFami TF314453.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 376890.
    PROi Q8VDB2.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q8VDB2.

    Family and domain databases

    InterProi IPR005599. GPI_mannosylTrfase.
    [Graphical view ]
    PANTHERi PTHR22760. PTHR22760. 1 hit.
    Pfami PF03901. Glyco_transf_22. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Common origin and evolution of glycosyltransferases using Dol-P-monosaccharides as donor substrate."
      Oriol R., Martinez-Duncker I., Chantret I., Mollicone R., Codogno P.
      Mol. Biol. Evol. 19:1451-1463(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: FVB/N.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Heart.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiALG12_MOUSE
    AccessioniPrimary (citable) accession number: Q8VDB2
    Secondary accession number(s): Q3UQK8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2004
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 82 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3