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Protein

General transcription factor IIH subunit 3

Gene

Gtf2h3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri269 – 286C4-typeAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 3
Alternative name(s):
Basic transcription factor 2 34 kDa subunit
Short name:
BTF2 p34
General transcription factor IIH polypeptide 3
TFIIH basal transcription factor complex p34 subunit
Gene namesi
Name:Gtf2h3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1277143 Gtf2h3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192521 – 309General transcription factor IIH subunit 3Add BLAST309

Proteomic databases

EPDiQ8VD76
MaxQBiQ8VD76
PaxDbiQ8VD76
PRIDEiQ8VD76

PTM databases

iPTMnetiQ8VD76
PhosphoSitePlusiQ8VD76

Expressioni

Gene expression databases

BgeeiENSMUSG00000029387
CleanExiMM_GTF2H3
ExpressionAtlasiQ8VD76 baseline and differential
GenevisibleiQ8VD76 MM

Interactioni

Subunit structurei

Part of a TFIID-containing RNA polymerase II pre-initiation complex that is composed of TBP and at least GTF2A1, GTF2A2, GTF2E1, GTF2E2, GTF2F1, GTF2H2, GTF2H3, GTF2H4, GTF2H5, GTF2B, TCEA1, ERCC2, ERCC3, TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription (By similarity). Interacts with RARA; the interaction requires prior phosphorylation of RARA on 'Ser-369' which then enhances interaction of RARA with CDK7 (PubMed:9230306).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi229071, 3 interactors
STRINGi10090.ENSMUSP00000031333

Structurei

3D structure databases

ProteinModelPortaliQ8VD76
SMRiQ8VD76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB4 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri269 – 286C4-typeAdd BLAST18

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG2487 Eukaryota
COG5242 LUCA
GeneTreeiENSGT00390000013143
HOGENOMiHOG000175900
HOVERGENiHBG054271
InParanoidiQ8VD76
KOiK03143
OMAiFGNAHLM
OrthoDBiEOG091G0GIC
PhylomeDBiQ8VD76
TreeFamiTF314336

Family and domain databases

Gene3Di3.40.50.410, 1 hit
InterProiView protein in InterPro
IPR004600 TFIIH_Tfb4/GTF2H3
IPR036465 vWFA_dom_sf
PANTHERiPTHR12831 PTHR12831, 1 hit
PfamiView protein in Pfam
PF03850 Tfb4, 1 hit
TIGRFAMsiTIGR00627 tfb4, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VD76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAADEDELNL LVIIVDTNPI WWGKQALKES QFTLSKCMDA VMVLANSHLF
60 70 80 90 100
MNRSNQLAVI ASHIQESRLL YPGKNGGLGD FFGDPGNALP DCNPSGSKDG
110 120 130 140 150
KYELLTVANE VIAEEIKDLM TKSDIKGQHT ETLLAGSLAK ALCYIHRVNK
160 170 180 190 200
AVKDNQEMKS RILVIKAAED SALQYMNFMN VIFAAQKQNI LIDACVLDSD
210 220 230 240 250
SGLLQQACDI TGGLYLKVPQ MPSLLQYLLW VFLPDQDQRS QLILPPPIHV
260 270 280 290 300
DYRAACFCHR SLIEIGYVCS VCLSIFCNFS PICTTCETAF KISLPPVLKA

KKKKQKVSL
Length:309
Mass (Da):34,244
Last modified:March 1, 2002 - v1
Checksum:i39CD6D1B8CD86E2D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC017515 mRNA Translation: AAH17515.1
CCDSiCCDS19680.1
RefSeqiNP_852075.1, NM_181410.3
UniGeneiMm.185467

Genome annotation databases

EnsembliENSMUST00000031333; ENSMUSP00000031333; ENSMUSG00000029387
GeneIDi209357
KEGGimmu:209357
UCSCiuc012edo.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTF2H3_MOUSE
AccessioniPrimary (citable) accession number: Q8VD76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: March 1, 2002
Last modified: March 28, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health