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Protein

Protein ABHD4

Gene

Abhd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Lysophospholipase selective for N-acyl phosphatidylethanolamine (NAPE). Contributes to the biosynthesis of N-acyl ethanolamines, including the endocannabinoid anandamide by hydrolyzing the sn-1 and sn-2 acyl chains from N-acyl phosphatidylethanolamine (NAPE) generating glycerophospho-N-acyl ethanolamine (GP-NAE), an intermediate for N-acyl ethanolamine biosynthesis. Hydrolyzes substrates bearing saturated, monounsaturated, polyunsaturated N-acyl chains. Shows no significant activity towards other lysophospholipids, including lysophosphatidylcholine, lysophosphatidylethanolamine and lysophosphatidylserine.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Protein family/group databases

ESTHERimouse-abhd4. CGI-58_ABHD5_ABHD4.
MEROPSiS33.013.

Chemistry

SwissLipidsiSLP:000001119.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein ABHD4Curated (EC:3.-.-.-)
Alternative name(s):
Alpha/beta hydrolase domain-containing protein 4Curated
Short name:
Abhydrolase domain-containing protein 4Imported
Alpha/beta-hydrolase 4
Lyso-N-acylphosphatidylethanolamine lipase
Gene namesi
Name:Abhd4Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1915938. Abhd4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Protein ABHD4PRO_0000080865Add
BLAST

Proteomic databases

EPDiQ8VD66.
MaxQBiQ8VD66.
PaxDbiQ8VD66.
PRIDEiQ8VD66.

PTM databases

iPTMnetiQ8VD66.
PhosphoSiteiQ8VD66.

Expressioni

Tissue specificityi

Highest levels in the CNS and in testis, intermediate levels in liver and kidney. Hardly detectable in heart.1 Publication

Gene expression databases

BgeeiQ8VD66.
CleanExiMM_ABHD4.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044134.

Structurei

3D structure databases

ProteinModelPortaliQ8VD66.
SMRiQ8VD66. Positions 64-268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4409. Eukaryota.
COG0596. LUCA.
HOVERGENiHBG054445.
InParanoidiQ8VD66.
KOiK13698.
PhylomeDBiQ8VD66.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VD66-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADDLEQQPQ GWLSSWLPTW RPTSMSQLKN VEARILQCLQ NKFLARYVSL
60 70 80 90 100
PNQNKIWTVT VSPEQKDRTP LVMVHGFGGG VGLWILNMDS LSARRTLHTF
110 120 130 140 150
DLLGFGRSSR PTFPRDPEGA EDEFVASIET WRETMGIPTM ILLGHSLGGF
160 170 180 190 200
LATSYSIKYP ERVKHLILVD PWGFPLRPTD PSEIRAPPTW VKAVASVLGR
210 220 230 240 250
SNPLAVLRVA GPWGPGLVQR FRPDFKRKFA DFFEDDTISE YIYHCNAQNP
260 270 280 290 300
SGETAFKAMM ESFGWARRPM LERIHLIRKD VPITMIYGAN TWIDTSTGKK
310 320 330 340
VKMQRPDSYV RDMEIEGASH HVYADQPHIF NAVVEEICNS VD
Length:342
Mass (Da):38,861
Last modified:March 1, 2002 - v1
Checksum:i902E8AA7133FFF99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049366 mRNA. Translation: BAC33711.1.
BC017532 mRNA. Translation: AAH17532.1.
RefSeqiNP_001192110.1. NM_001205181.1.
NP_598837.2. NM_134076.2.
UniGeneiMm.28771.

Genome annotation databases

GeneIDi105501.
KEGGimmu:105501.
UCSCiuc007tvo.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049366 mRNA. Translation: BAC33711.1.
BC017532 mRNA. Translation: AAH17532.1.
RefSeqiNP_001192110.1. NM_001205181.1.
NP_598837.2. NM_134076.2.
UniGeneiMm.28771.

3D structure databases

ProteinModelPortaliQ8VD66.
SMRiQ8VD66. Positions 64-268.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000044134.

Chemistry

SwissLipidsiSLP:000001119.

Protein family/group databases

ESTHERimouse-abhd4. CGI-58_ABHD5_ABHD4.
MEROPSiS33.013.

PTM databases

iPTMnetiQ8VD66.
PhosphoSiteiQ8VD66.

Proteomic databases

EPDiQ8VD66.
MaxQBiQ8VD66.
PaxDbiQ8VD66.
PRIDEiQ8VD66.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi105501.
KEGGimmu:105501.
UCSCiuc007tvo.2. mouse.

Organism-specific databases

CTDi63874.
MGIiMGI:1915938. Abhd4.

Phylogenomic databases

eggNOGiKOG4409. Eukaryota.
COG0596. LUCA.
HOVERGENiHBG054445.
InParanoidiQ8VD66.
KOiK13698.
PhylomeDBiQ8VD66.

Miscellaneous databases

NextBioi357734.
PROiQ8VD66.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VD66.
CleanExiMM_ABHD4.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  3. "Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta hydrolase 4 in this pathway."
    Simon G.M., Cravatt B.F.
    J. Biol. Chem. 281:26465-26472(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Lung and Testis.

Entry informationi

Entry nameiABHD4_MOUSE
AccessioniPrimary (citable) accession number: Q8VD66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2002
Last modified: May 11, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Thr-291 is present instead of the conserved His which is expected to be an active site residue.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.