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Protein

Alkaline ceramidase 2

Gene

Acer2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.By similarity

Catalytic activityi

N-acylsphingosine + H2O = a carboxylate + sphingosine.

Enzyme regulationi

Specifically activated by lumenal, but not cytosolic Ca2+.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism

Enzyme and pathway databases

BRENDAi3.5.1.23. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase 2 (EC:3.5.1.23)
Short name:
AlkCDase 2
Short name:
Alkaline CDase 2
Short name:
maCER2
Alternative name(s):
Acylsphingosine deacylase 3-like
Cancer-related gene liver 1 protein
Short name:
CRG-L1
N-acylsphingosine amidohydrolase 3-like
Gene namesi
Name:Acer2
Synonyms:Asah3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1920932. Acer2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232LumenalSequence analysisAdd
BLAST
Transmembranei33 – 5321HelicalSequence analysisAdd
BLAST
Topological domaini54 – 629CytoplasmicSequence analysis
Transmembranei63 – 8321HelicalSequence analysisAdd
BLAST
Topological domaini84 – 863LumenalSequence analysis
Transmembranei87 – 10721HelicalSequence analysisAdd
BLAST
Topological domaini108 – 12417CytoplasmicSequence analysisAdd
BLAST
Transmembranei125 – 14218HelicalSequence analysisAdd
BLAST
Topological domaini143 – 1431LumenalSequence analysis
Transmembranei144 – 16421HelicalSequence analysisAdd
BLAST
Topological domaini165 – 1739CytoplasmicSequence analysis
Transmembranei174 – 19421HelicalSequence analysisAdd
BLAST
Topological domaini195 – 21117LumenalSequence analysisAdd
BLAST
Transmembranei212 – 23221HelicalSequence analysisAdd
BLAST
Topological domaini233 – 27543CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 275275Alkaline ceramidase 2PRO_0000247749Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi23 – 231N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VD53.
PRIDEiQ8VD53.

Expressioni

Gene expression databases

BgeeiQ8VD53.
CleanExiMM_ASAH3L.
GenevisibleiQ8VD53. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040048.

Structurei

3D structure databases

ProteinModelPortaliQ8VD53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline ceramidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000110920.
HOGENOMiHOG000220878.
InParanoidiQ8VD53.
KOiK01441.
OMAiSERWAFI.
OrthoDBiEOG7F5127.
PhylomeDBiQ8VD53.
TreeFamiTF313019.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VD53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAPHWWDHL RAGSSEVDWC EDNYTIVPAI AEFYNTISNV LFFILPPICM
60 70 80 90 100
CLFRQYATCF NSGIYLIWTL LVVVGIGSVY FHATLSFLGQ MLDELAILWV
110 120 130 140 150
LMCALAMWFP RRYLPKIFRN DRGRFKAVVC VLSAITTCLA FIKPAINNIS
160 170 180 190 200
LMILGLPCTA LLVAELKRCD NVRVFKLGLF SGLWWTLALF CWISDQAFCE
210 220 230 240 250
LLSSFHFPYL HCVWHILICL ASYLGCVCFA YFDAASEIPE QGPVIRFWPS
260 270
EKWAFIGVPY VSLLCAHKKS PVKIT
Length:275
Mass (Da):31,369
Last modified:March 1, 2002 - v1
Checksum:i8FCC755B4949B9DF
GO
Isoform 2 (identifier: Q8VD53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-167: Missing.

Note: No experimental confirmation available.
Show »
Length:229
Mass (Da):26,500
Checksum:iD396EFEA1F3B70A1
GO

Sequence cautioni

The sequence BAC39416.1 differs from that shown.Chimera.Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei122 – 16746Missing in isoform 2. 1 PublicationVSP_020036Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282864 mRNA. Translation: AAL40408.1.
AY312515 mRNA. Translation: AAQ85131.1.
BC059819 mRNA. Translation: AAH59819.1.
AK085306 mRNA. Translation: BAC39416.1. Sequence problems.
CCDSiCCDS18311.1. [Q8VD53-1]
CCDS71417.1. [Q8VD53-2]
RefSeqiNP_001277470.1. NM_001290541.1. [Q8VD53-2]
NP_001277472.1. NM_001290543.1.
NP_647467.1. NM_139306.3. [Q8VD53-1]
UniGeneiMm.45019.
Mm.458684.

Genome annotation databases

EnsembliENSMUST00000045224; ENSMUSP00000040048; ENSMUSG00000038007. [Q8VD53-1]
ENSMUST00000084433; ENSMUSP00000081473; ENSMUSG00000038007. [Q8VD53-2]
GeneIDi230379.
KEGGimmu:230379.
UCSCiuc008tmf.3. mouse. [Q8VD53-1]
uc012dgs.2. mouse. [Q8VD53-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282864 mRNA. Translation: AAL40408.1.
AY312515 mRNA. Translation: AAQ85131.1.
BC059819 mRNA. Translation: AAH59819.1.
AK085306 mRNA. Translation: BAC39416.1. Sequence problems.
CCDSiCCDS18311.1. [Q8VD53-1]
CCDS71417.1. [Q8VD53-2]
RefSeqiNP_001277470.1. NM_001290541.1. [Q8VD53-2]
NP_001277472.1. NM_001290543.1.
NP_647467.1. NM_139306.3. [Q8VD53-1]
UniGeneiMm.45019.
Mm.458684.

3D structure databases

ProteinModelPortaliQ8VD53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040048.

Proteomic databases

PaxDbiQ8VD53.
PRIDEiQ8VD53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045224; ENSMUSP00000040048; ENSMUSG00000038007. [Q8VD53-1]
ENSMUST00000084433; ENSMUSP00000081473; ENSMUSG00000038007. [Q8VD53-2]
GeneIDi230379.
KEGGimmu:230379.
UCSCiuc008tmf.3. mouse. [Q8VD53-1]
uc012dgs.2. mouse. [Q8VD53-2]

Organism-specific databases

CTDi340485.
MGIiMGI:1920932. Acer2.

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000110920.
HOGENOMiHOG000220878.
InParanoidiQ8VD53.
KOiK01441.
OMAiSERWAFI.
OrthoDBiEOG7F5127.
PhylomeDBiQ8VD53.
TreeFamiTF313019.

Enzyme and pathway databases

BRENDAi3.5.1.23. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

NextBioi379885.
PROiQ8VD53.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VD53.
CleanExiMM_ASAH3L.
GenevisibleiQ8VD53. MM.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression profiling and identification of novel genes in hepatocellular carcinomas."
    Graveel C.R., Jatkoe T., Madore S.J., Holt A.L., Farnham P.J.
    Oncogene 20:2704-2712(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Hepatoma.
  2. "Cloning and characterization of a Homo sapiens Golgi alkaline ceramidase: an enzyme that regulates the levels of sphingosine-1-P, and cell growth and survival."
    Xu R., Hu W., Obeid L.M., Mao C.
    Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: C57BL/6J.
    Tissue: Brain.
  4. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-215.
    Strain: C57BL/6J.
    Tissue: Kidney.

Entry informationi

Entry nameiACER2_MOUSE
AccessioniPrimary (citable) accession number: Q8VD53
Secondary accession number(s): Q6PB92, Q8BUG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2002
Last modified: November 11, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Up-regulated in hepatocellular carcinomas.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.