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Protein

Alkaline ceramidase 2

Gene

Acer2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid. Unsaturated long-chain ceramides are the best substrates, saturated long-chain ceramides and unsaturated very long-chain ceramides are good substrates, whereas saturated very long-chain ceramides and short-chain ceramides were poor substrates. Inhibits the maturation of protein glycosylation in the Golgi complex, including that of integrin beta-1 (ITGB1) and of LAMP1, by increasing the levels of sphingosine. Inhibits cell adhesion by reducing the level of ITGB1 in the cell surface. May have a role in cell proliferation and apoptosis that seems to depend on the balance between sphingosine and sphingosine-1-phosphate.By similarity

Catalytic activityi

N-acylsphingosine + H2O = a carboxylate + sphingosine.

Enzyme regulationi

Specifically activated by lumenal, but not cytosolic Ca2+.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid metabolism

Enzyme and pathway databases

BRENDAi3.5.1.23. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline ceramidase 2 (EC:3.5.1.23)
Short name:
AlkCDase 2
Short name:
Alkaline CDase 2
Short name:
maCER2
Alternative name(s):
Acylsphingosine deacylase 3-like
Cancer-related gene liver 1 protein
Short name:
CRG-L1
N-acylsphingosine amidohydrolase 3-like
Gene namesi
Name:Acer2
Synonyms:Asah3l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1920932. Acer2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 32LumenalSequence analysisAdd BLAST32
Transmembranei33 – 53HelicalSequence analysisAdd BLAST21
Topological domaini54 – 62CytoplasmicSequence analysis9
Transmembranei63 – 83HelicalSequence analysisAdd BLAST21
Topological domaini84 – 86LumenalSequence analysis3
Transmembranei87 – 107HelicalSequence analysisAdd BLAST21
Topological domaini108 – 124CytoplasmicSequence analysisAdd BLAST17
Transmembranei125 – 142HelicalSequence analysisAdd BLAST18
Topological domaini143LumenalSequence analysis1
Transmembranei144 – 164HelicalSequence analysisAdd BLAST21
Topological domaini165 – 173CytoplasmicSequence analysis9
Transmembranei174 – 194HelicalSequence analysisAdd BLAST21
Topological domaini195 – 211LumenalSequence analysisAdd BLAST17
Transmembranei212 – 232HelicalSequence analysisAdd BLAST21
Topological domaini233 – 275CytoplasmicSequence analysisAdd BLAST43

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002477491 – 275Alkaline ceramidase 2Add BLAST275

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi23N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ8VD53.
PRIDEiQ8VD53.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038007.
CleanExiMM_ASAH3L.
GenevisibleiQ8VD53. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040048.

Structurei

3D structure databases

ProteinModelPortaliQ8VD53.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline ceramidase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000110920.
HOGENOMiHOG000220878.
InParanoidiQ8VD53.
KOiK01441.
OMAiKFWPSER.
OrthoDBiEOG091G0HT1.
PhylomeDBiQ8VD53.
TreeFamiTF313019.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VD53-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAPHWWDHL RAGSSEVDWC EDNYTIVPAI AEFYNTISNV LFFILPPICM
60 70 80 90 100
CLFRQYATCF NSGIYLIWTL LVVVGIGSVY FHATLSFLGQ MLDELAILWV
110 120 130 140 150
LMCALAMWFP RRYLPKIFRN DRGRFKAVVC VLSAITTCLA FIKPAINNIS
160 170 180 190 200
LMILGLPCTA LLVAELKRCD NVRVFKLGLF SGLWWTLALF CWISDQAFCE
210 220 230 240 250
LLSSFHFPYL HCVWHILICL ASYLGCVCFA YFDAASEIPE QGPVIRFWPS
260 270
EKWAFIGVPY VSLLCAHKKS PVKIT
Length:275
Mass (Da):31,369
Last modified:March 1, 2002 - v1
Checksum:i8FCC755B4949B9DF
GO
Isoform 2 (identifier: Q8VD53-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     122-167: Missing.

Note: No experimental confirmation available.
Show »
Length:229
Mass (Da):26,500
Checksum:iD396EFEA1F3B70A1
GO

Sequence cautioni

The sequence BAC39416 differs from that shown. Chimera.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020036122 – 167Missing in isoform 2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282864 mRNA. Translation: AAL40408.1.
AY312515 mRNA. Translation: AAQ85131.1.
BC059819 mRNA. Translation: AAH59819.1.
AK085306 mRNA. Translation: BAC39416.1. Sequence problems.
CCDSiCCDS18311.1. [Q8VD53-1]
CCDS71417.1. [Q8VD53-2]
RefSeqiNP_001277470.1. NM_001290541.1. [Q8VD53-2]
NP_001277472.1. NM_001290543.1.
NP_647467.1. NM_139306.3. [Q8VD53-1]
UniGeneiMm.45019.
Mm.458684.

Genome annotation databases

EnsembliENSMUST00000045224; ENSMUSP00000040048; ENSMUSG00000038007. [Q8VD53-1]
ENSMUST00000084433; ENSMUSP00000081473; ENSMUSG00000038007. [Q8VD53-2]
GeneIDi230379.
KEGGimmu:230379.
UCSCiuc008tmf.3. mouse. [Q8VD53-1]
uc012dgs.2. mouse. [Q8VD53-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF282864 mRNA. Translation: AAL40408.1.
AY312515 mRNA. Translation: AAQ85131.1.
BC059819 mRNA. Translation: AAH59819.1.
AK085306 mRNA. Translation: BAC39416.1. Sequence problems.
CCDSiCCDS18311.1. [Q8VD53-1]
CCDS71417.1. [Q8VD53-2]
RefSeqiNP_001277470.1. NM_001290541.1. [Q8VD53-2]
NP_001277472.1. NM_001290543.1.
NP_647467.1. NM_139306.3. [Q8VD53-1]
UniGeneiMm.45019.
Mm.458684.

3D structure databases

ProteinModelPortaliQ8VD53.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000040048.

Proteomic databases

PaxDbiQ8VD53.
PRIDEiQ8VD53.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045224; ENSMUSP00000040048; ENSMUSG00000038007. [Q8VD53-1]
ENSMUST00000084433; ENSMUSP00000081473; ENSMUSG00000038007. [Q8VD53-2]
GeneIDi230379.
KEGGimmu:230379.
UCSCiuc008tmf.3. mouse. [Q8VD53-1]
uc012dgs.2. mouse. [Q8VD53-2]

Organism-specific databases

CTDi340485.
MGIiMGI:1920932. Acer2.

Phylogenomic databases

eggNOGiKOG2329. Eukaryota.
ENOG4111RPN. LUCA.
GeneTreeiENSGT00730000110920.
HOGENOMiHOG000220878.
InParanoidiQ8VD53.
KOiK01441.
OMAiKFWPSER.
OrthoDBiEOG091G0HT1.
PhylomeDBiQ8VD53.
TreeFamiTF313019.

Enzyme and pathway databases

BRENDAi3.5.1.23. 3474.
ReactomeiR-MMU-1660661. Sphingolipid de novo biosynthesis.

Miscellaneous databases

PROiQ8VD53.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038007.
CleanExiMM_ASAH3L.
GenevisibleiQ8VD53. MM.

Family and domain databases

InterProiIPR008901. Ceramidase.
[Graphical view]
PfamiPF05875. Ceramidase. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACER2_MOUSE
AccessioniPrimary (citable) accession number: Q8VD53
Secondary accession number(s): Q6PB92, Q8BUG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2006
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Up-regulated in hepatocellular carcinomas.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.