Reviewed, UniProtKB/Swiss-Prot Q8VD52 (PLPP_RAT)

Names and origin

Protein names

Recommended name:
    Pyridoxal phosphate phosphatase
      Short name=PLP phosphatase
    EC=3.1.3.74
Alternative name(s):
    Reg I-binding protein 1

Gene names

Name:	Pdxp
Synonyms:	Plp, Plpp, Rbp1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and Pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP By similarity.
Catalytic activity	Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.
Cofactor	Divalent ions. Magnesium is the most effective By similarity.
Subunit structure	Homodimer By similarity. Interacts with REG1.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW pyridoxal phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Pyridoxal phosphate phosphatase		PRO_0000068839
Sites
	Active site	25	1	Nucleophile By similarity
	Active site	27	1	Proton donor By similarity
	Metal binding	25	1	Magnesium By similarity
	Metal binding	27	1	Magnesium By similarity
	Metal binding	234	1	Magnesium By similarity
Experimental info
	Sequence conflict	106 – 108	3	APG → GTR Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q8VD52-1 [UniParc]. Last modified January 10, 2006. Version 2. Checksum: E609DAA5250151C4	FASTA	309	33,115
	10 20 30 40 50 60 MARCERLRGA ALRDVLGQAQ GVLFDCDGVL WNGERIVPGA PELLQRLAQA GKATLFVSNN 70 80 90 100 110 120 SRRARPELAL RFARLGFTGL RAEELFSSAV CAARLLRQRL PGPPDAPGAV FVLGGEGLRA 130 140 150 160 170 180 ELRAAGLRLA GDPGDDPRVR AVLVGYDEHF SFAKLTEACA HLRDPDCLLV ATDRDPWHPL 190 200 210 220 230 240 TDGSRTPGTG SLAAAVETAS GRQALVVGKP SPYMFQCITE DFSVDPARML MVGDRLETDI 250 260 270 280 290 300 LFGHRCGMTT VLTLTGVSSL EEAQAYLAAG QHDLVPHYYV ESIADLMEGL GGLSPPPQFP DPVDGGYRP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence of the Brown Norway rat yields insights into mammalian evolution." Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M. , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S. Nature 428:493-521(2004) [PubMed: 15057822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Brown Norway.
[2]	"Isolation of cDNA clone from rat pancreas cDNA library by two hybrid system, clone 13." Wu H., Zenilman M.E. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 106-309. Strain: Wistar. Tissue: Pancreas.
[3]	Lubec G., Chen W.-Q. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 129-138; 141-154; 186-202 AND 236-245, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Hippocampus.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AABR03056024 Genomic DNA. No translation available. AF318578 mRNA. Translation: AAL37168.1.
IPI	IPI00361415.
UniGene	Rn.9374
3D structure databases
SMR	Q8VD52. Positions 2-290.
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000009570. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	1586212. Pdxp.
Phylogenomic databases
HOVERGEN	Q8VD52.
Enzyme and pathway databases
BRENDA	3.1.3.74. 248.
Gene expression databases
ArrayExpress	Q8VD52.
GermOnline	ENSRNOG00000009570. Rattus norvegicus.
Family and domain databases
InterPro	IPR005834. Dehalogen-like_hydro. IPR006357. HAD-SF_hydro_IIA. IPR006349. PGP_euk. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01460. HAD-SF-IIA. 1 hit. TIGR01452. PGP_euk. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLPP_RAT

Accession

Primary (citable) accession number: Q8VD52

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	January 10, 2006
Last modified:	February 10, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)