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Protein

Pyridoxal phosphate phosphatase

Gene

Pdxp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein serine phosphatase that dephosphorylates 'Ser-3' in cofilin and probably also dephosphorylates phospho-serine residues in DSTN. Regulates cofilin-dependent actin cytoskeleton reorganization. Required for normal progress through mitosis and normal cytokinesis. Does not dephosphorylate phospho-threonines in LIMK1. Does not dephosphorylate peptides containing phospho-tyrosine. Pyridoxal phosphate phosphatase. Has some activity towards pyridoxal 5'-phosphate (PLP), pyridoxine 5'-phosphate (PMP) and pyridoxine 5'-phosphate (PNP), with a highest activity with PLP followed by PNP.By similarity

Catalytic activityi

Pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate.By similarity
O-phospho-L(or D)-serine + H2O = L(or D)-serine + phosphate.By similarity

Cofactori

Mg2+By similarityNote: Divalent metal ions. Mg2+ is the most effective.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei25 – 251NucleophileBy similarity
Metal bindingi25 – 251MagnesiumBy similarity
Active sitei27 – 271Proton donorBy similarity
Metal bindingi27 – 271Magnesium; via carbonyl oxygenBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei209 – 2091SubstrateBy similarity
Metal bindingi234 – 2341MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal phosphate phosphatase (EC:3.1.3.3By similarity, EC:3.1.3.74By similarity)
Short name:
PLP phosphatase
Alternative name(s):
Chronophin
Reg I-binding protein 1
Gene namesi
Name:Pdxp
Synonyms:Cin, Plp, Plpp, Rbp1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1586212. Pdxp.

Subcellular locationi

  • Cytoplasmcytosol By similarity
  • Cytoplasmcytoskeleton By similarity
  • Cell projectionruffle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell projectionlamellipodium membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Colocalizes with the actin cytoskeleton in membrane ruffles and lamellipodia. Diffusely distributed throughout the cytosol during pro-metaphase and metaphase. Detected at the dynamic cell poles during telophase. Detected at the cleavage furrow and contractile ring during cytokinesis. Transiently detected at the plasma membrane in late stages of cytokinesis. Detected at the midbody.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Pyridoxal phosphate phosphatasePRO_0000068839Add
BLAST

Proteomic databases

PaxDbiQ8VD52.
PRIDEiQ8VD52.

Expressioni

Gene expression databases

BgeeiENSRNOG00000009570.

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with REG1.By similarity

GO - Molecular functioni

  • growth factor binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064092.

Structurei

3D structure databases

ProteinModelPortaliQ8VD52.
SMRiQ8VD52. Positions 2-290.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni58 – 625Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the HAD-like hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiKOG2882. Eukaryota.
COG0647. LUCA.
HOGENOMiHOG000068104.
HOVERGENiHBG049429.
InParanoidiQ8VD52.
PhylomeDBiQ8VD52.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
PfamiPF13344. Hydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF000915. PGP-type_phosphatase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VD52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MARCERLRGA ALRDVLGQAQ GVLFDCDGVL WNGERIVPGA PELLQRLAQA
60 70 80 90 100
GKATLFVSNN SRRARPELAL RFARLGFTGL RAEELFSSAV CAARLLRQRL
110 120 130 140 150
PGPPDAPGAV FVLGGEGLRA ELRAAGLRLA GDPGDDPRVR AVLVGYDEHF
160 170 180 190 200
SFAKLTEACA HLRDPDCLLV ATDRDPWHPL TDGSRTPGTG SLAAAVETAS
210 220 230 240 250
GRQALVVGKP SPYMFQCITE DFSVDPARML MVGDRLETDI LFGHRCGMTT
260 270 280 290 300
VLTLTGVSSL EEAQAYLAAG QHDLVPHYYV ESIADLMEGL GGLSPPPQFP

DPVDGGYRP
Length:309
Mass (Da):33,115
Last modified:January 10, 2006 - v2
Checksum:iE609DAA5250151C4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti106 – 1083APG → GTR (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03056024 Genomic DNA. No translation available.
AF318578 mRNA. Translation: AAL37168.1.
UniGeneiRn.222285.

Genome annotation databases

UCSCiRGD:1586212. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03056024 Genomic DNA. No translation available.
AF318578 mRNA. Translation: AAL37168.1.
UniGeneiRn.222285.

3D structure databases

ProteinModelPortaliQ8VD52.
SMRiQ8VD52. Positions 2-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000064092.

Proteomic databases

PaxDbiQ8VD52.
PRIDEiQ8VD52.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:1586212. rat.

Organism-specific databases

RGDi1586212. Pdxp.

Phylogenomic databases

eggNOGiKOG2882. Eukaryota.
COG0647. LUCA.
HOGENOMiHOG000068104.
HOVERGENiHBG049429.
InParanoidiQ8VD52.
PhylomeDBiQ8VD52.

Miscellaneous databases

PROiQ8VD52.

Gene expression databases

BgeeiENSRNOG00000009570.

Family and domain databases

Gene3Di3.40.50.1000. 2 hits.
3.40.50.10410. 1 hit.
InterProiIPR023214. HAD-like_dom.
IPR006357. HAD-SF_hydro_IIA.
IPR023215. NPhePase-like_dom.
IPR006349. PGP_euk.
[Graphical view]
PfamiPF13344. Hydrolase_6. 1 hit.
[Graphical view]
PIRSFiPIRSF000915. PGP-type_phosphatase. 1 hit.
SUPFAMiSSF56784. SSF56784. 1 hit.
TIGRFAMsiTIGR01460. HAD-SF-IIA. 1 hit.
TIGR01452. PGP_euk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPLPP_RAT
AccessioniPrimary (citable) accession number: Q8VD52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: January 10, 2006
Last modified: September 7, 2016
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.