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Protein

Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1

Gene

Asz1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Its association with pi-bodies suggests a participation in the primary piRNAs metabolic process. Required prior to the pachytene stage to facilitate the production of multiple types of piRNAs, including those associated with repeats involved in regulation of retrotransposons. May act by mediating protein-protein interactions during germ cell maturation.1 Publication

GO - Molecular functioni

  • signal transducer activity Source: UniProtKB

GO - Biological processi

  • DNA methylation involved in gamete generation Source: UniProtKB
  • gene silencing by RNA Source: UniProtKB-KW
  • germ cell development Source: UniProtKB
  • male meiosis Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • piRNA metabolic process Source: UniProtKB
  • spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Meiosis, RNA-mediated gene silencing, Spermatogenesis

Enzyme and pathway databases

ReactomeiR-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1
Alternative name(s):
Germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein
Gene namesi
Name:Asz1Imported
Synonyms:GaszImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1921318. Asz1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: Reactome
  • pi-body Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Mice are viable but show profound defect in male meiosis leading to male sterility. Testes display increased hypomethylation of retrotransposons and their subsequent expression as well as piRNAs suppression.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1PRO_0000066970Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171PhosphoserineCombined sources
Modified residuei18 – 181PhosphoserineCombined sources
Modified residuei20 – 201PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VD46.
PaxDbiQ8VD46.
PRIDEiQ8VD46.

PTM databases

iPTMnetiQ8VD46.
PhosphoSiteiQ8VD46.

Expressioni

Tissue specificityi

Expressed exclusively in testis and ovary with higher levels in testis.1 Publication

Developmental stagei

Expressed in pachytene spermatocytes and early spermatids in the developing and adult testes and in oocytes in all stages of oogenesis in the ovary. Also expressed in preimplantive embryos.1 Publication

Gene expression databases

BgeeiENSMUSG00000010796.
ExpressionAtlasiQ8VD46. baseline and differential.
GenevisibleiQ8VD46. MM.

Interactioni

Subunit structurei

Interacts with DDX4, PIWIL1, RANBP9 and TDRD1.1 Publication

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010940.

Structurei

3D structure databases

ProteinModelPortaliQ8VD46.
SMRiQ8VD46. Positions 28-234.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati45 – 7430ANK 1CuratedAdd
BLAST
Repeati78 – 10730ANK 2CuratedAdd
BLAST
Repeati110 – 14435ANK 3CuratedAdd
BLAST
Repeati148 – 17730ANK 4CuratedAdd
BLAST
Repeati181 – 21030ANK 5CuratedAdd
BLAST
Repeati214 – 24330ANK 6CuratedAdd
BLAST
Domaini272 – 33463SAMCuratedAdd
BLAST

Sequence similaritiesi

Contains 6 ANK repeats.PROSITE-ProRule annotationCurated

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129724.
HOGENOMiHOG000034114.
HOVERGENiHBG050599.
InParanoidiQ8VD46.
KOiK18410.
OMAiFTKNGIT.
OrthoDBiEOG091G0TMP.
TreeFamiTF352216.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 5 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VD46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGTLRGLA VAGGGESSDS EDDGWDIGYL DRSSQKLKRS LPVEEKNETF
60 70 80 90 100
KKALTTGDIS LVKELLDSGI NVDSSFRYGW TPLMYAASVA NAELVRFLLD
110 120 130 140 150
RGANASFDKD KLTILISACS ARGSEEQVLK CVELLLSRNA DPNTACRRLM
160 170 180 190 200
TPIMYAARDG HTQVVALLVA HGAEVNAQDE NGYTALTWAA RQGHKNVILK
210 220 230 240 250
LLELGANKML QTKDGRTPSE IAKRNKHLEI FNFLSLTLNP LEGKLQQLTK
260 270 280 290 300
EETICKLLAT DSDKEKDHIF SPYTAFGDLE IFLHGLGLEH MTDSLKEKDI
310 320 330 340 350
TLRHLLTMKK DELTKNGIAS KDQQKILAAL KELEVEEINF GKLPEVTKLE
360 370 380 390 400
ISGDEFLNFL LKLNKQCGHL ITAVQNIITE LPVNSHKIVL EWASPRNFTS
410 420 430 440 450
VCEELVSNVE DLNEEVCRLK ELIQKMQNER ENDPTHIPLV EEVSTWKTRI
460 470
LKRSAVTVCG FGLLLFIGKL TLQRK
Length:475
Mass (Da):52,984
Last modified:April 18, 2012 - v2
Checksum:i8B3BA5F357B26732
GO

Sequence cautioni

The sequence AAF30297 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471N → T in AAL67487 (PubMed:12040005).Curated
Sequence conflicti249 – 2491T → A in AAH58859 (PubMed:15489334).Curated
Sequence conflicti352 – 3521S → R in AAF30297 (PubMed:10655503).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF459789 mRNA. Translation: AAL67487.1.
AF162137 Genomic DNA. Translation: AAF30297.1. Sequence problems.
AC068561 Genomic DNA. No translation available.
AC158647 Genomic DNA. No translation available.
CH466533 Genomic DNA. Translation: EDL13867.1.
BC058859 mRNA. Translation: AAH58859.1.
CCDSiCCDS19929.1.
RefSeqiNP_076218.3. NM_023729.3.
UniGeneiMm.73091.

Genome annotation databases

EnsembliENSMUST00000010940; ENSMUSP00000010940; ENSMUSG00000010796.
GeneIDi74068.
KEGGimmu:74068.
UCSCiuc009bah.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF459789 mRNA. Translation: AAL67487.1.
AF162137 Genomic DNA. Translation: AAF30297.1. Sequence problems.
AC068561 Genomic DNA. No translation available.
AC158647 Genomic DNA. No translation available.
CH466533 Genomic DNA. Translation: EDL13867.1.
BC058859 mRNA. Translation: AAH58859.1.
CCDSiCCDS19929.1.
RefSeqiNP_076218.3. NM_023729.3.
UniGeneiMm.73091.

3D structure databases

ProteinModelPortaliQ8VD46.
SMRiQ8VD46. Positions 28-234.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000010940.

PTM databases

iPTMnetiQ8VD46.
PhosphoSiteiQ8VD46.

Proteomic databases

MaxQBiQ8VD46.
PaxDbiQ8VD46.
PRIDEiQ8VD46.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010940; ENSMUSP00000010940; ENSMUSG00000010796.
GeneIDi74068.
KEGGimmu:74068.
UCSCiuc009bah.2. mouse.

Organism-specific databases

CTDi136991.
MGIiMGI:1921318. Asz1.

Phylogenomic databases

eggNOGiKOG0504. Eukaryota.
COG0666. LUCA.
GeneTreeiENSGT00840000129724.
HOGENOMiHOG000034114.
HOVERGENiHBG050599.
InParanoidiQ8VD46.
KOiK18410.
OMAiFTKNGIT.
OrthoDBiEOG091G0TMP.
TreeFamiTF352216.

Enzyme and pathway databases

ReactomeiR-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.

Miscellaneous databases

PROiQ8VD46.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000010796.
ExpressionAtlasiQ8VD46. baseline and differential.
GenevisibleiQ8VD46. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
1.25.40.20. 1 hit.
InterProiIPR002110. Ankyrin_rpt.
IPR020683. Ankyrin_rpt-contain_dom.
IPR001660. SAM.
IPR013761. SAM/pointed.
[Graphical view]
PfamiPF12796. Ank_2. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
PRINTSiPR01415. ANKYRIN.
SMARTiSM00248. ANK. 5 hits.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF48403. SSF48403. 1 hit.
PROSITEiPS50297. ANK_REP_REGION. 1 hit.
PS50088. ANK_REPEAT. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASZ1_MOUSE
AccessioniPrimary (citable) accession number: Q8VD46
Secondary accession number(s): G3X8S0, Q6PD92, Q9JKQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2003
Last sequence update: April 18, 2012
Last modified: September 7, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.