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Protein

GRIP1-associated protein 1

Gene

Gripap1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulates the endosomal recycling back to the neuronal plasma membrane, possibly by connecting early and late recycling endosomal domains and promoting segregation of recycling endosomes from early endosomal membranes. Involved in the localization of recycling endosomes to dendritic spines, thereby playing a role in the maintenance of dendritic spine morphology. Required for the activity-induced AMPA receptor recycling to dendrite membranes and for long-term potentiation and synaptic plasticity (By similarity).By similarity
GRASP-1 C-terminal chain: Functions as a scaffold protein to facilitate MAP3K1/MEKK1-mediated activation of the JNK1 kinase by phosphorylation, possibly by bringing MAP3K1/MEKK1 and JNK1 in close proximity (By similarity).By similarity

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
GRIP1-associated protein 1
Short name:
GRASP-1
Alternative name(s):
HCMV-interacting protein
Cleaved into the following chain:
GRASP-1 C-terminal chainBy similarity
Alternative name(s):
30kDa C-terminus form
Gene namesi
Name:Gripap1
Synonyms:DXImx47e, Kiaa1167
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1859616. Gripap1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell projection, Endosome, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000875822 – 806GRIP1-associated protein 1Add BLAST805
ChainiPRO_0000441812564 – 806GRASP-1 C-terminal chainAdd BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei620PhosphoserineCombined sources1
Modified residuei631PhosphoserineCombined sources1
Modified residuei633PhosphoserineCombined sources1
Modified residuei634PhosphoserineCombined sources1
Modified residuei653PhosphoserineBy similarity1
Modified residuei655PhosphoserineBy similarity1
Modified residuei656PhosphoserineCombined sources1
Modified residuei657PhosphoserineCombined sources1
Modified residuei795PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically cleaved by caspase-3. A minor C-terminal proteolytic fragment of 30 kDa is produced. Proteolytic cleavage is required for JNK signaling activation.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei563 – 564Cleavage; by caspase-3By similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ8VD04.
MaxQBiQ8VD04.
PaxDbiQ8VD04.
PeptideAtlasiQ8VD04.
PRIDEiQ8VD04.

PTM databases

iPTMnetiQ8VD04.
PhosphoSitePlusiQ8VD04.

Expressioni

Tissue specificityi

Expressed in the central nervous system, including cortex, cerebellum, midbrain and spinal cord, and in primary cultured hippocampal neurons but absent in non-neuronal tissues and cell types with the exception of neuroendocrine insulinoma cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000031153.
CleanExiMM_GRIPAP1.
ExpressionAtlasiQ8VD04. baseline and differential.
GenevisibleiQ8VD04. MM.

Interactioni

Subunit structurei

Interacts with GRIP1, GRIP2 and AMPA receptors. Interacts (via C-terminus) with MAPK8/JNK1 and MAP3K1/MEKK1; the interaction promotes MAP3K1-mediated phosphorylation of MAPK8. Interacts (via N-terminus) with RAB4A (in GTP-bound form) (By similarity). Interacts (via C-terminus) with STX12 (PubMed:20098723).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Mapk8A3KBF52EBI-7585099,EBI-7585074

Protein-protein interaction databases

IntActiQ8VD04. 3 interactors.
MINTiQ8VD04.
STRINGi10090.ENSMUSP00000068789.

Structurei

3D structure databases

ProteinModelPortaliQ8VD04.
SMRiQ8VD04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili4 – 158Sequence analysisAdd BLAST155
Coiled coili204 – 606Sequence analysisAdd BLAST403
Coiled coili666 – 700Sequence analysisAdd BLAST35
Coiled coili750 – 779Sequence analysisAdd BLAST30

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IKP9. Eukaryota.
ENOG410YB89. LUCA.
GeneTreeiENSGT00720000108868.
HOGENOMiHOG000231369.
HOVERGENiHBG080243.
InParanoidiQ8VD04.
PhylomeDBiQ8VD04.
TreeFamiTF329006.

Family and domain databases

InterProiView protein in InterPro
IPR026204. GRIPAP1.
PANTHERiPTHR18978. PTHR18978. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VD04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQALSEEEF QRMQTQLLEL RTNNYQLSDE LRKNGVELSS LRQKVAYLDK
60 70 80 90 100
EFSKAQKALS KSKKAQEVEV LLSENEMLQA KLHSQEEDFR LQNSTLMAEF
110 120 130 140 150
SKLCSQLEQL ELENRQLKEG VPGAAGAHVD GELLRLQAEN TALQKNMAAL
160 170 180 190 200
QERYGKEAVR PSAVGEGQGD PPGDVLPTPL APMPLAEVEL KWEMEREEKK
210 220 230 240 250
LLWEQLQGLE SSKQAETSRL QEELAKLSEK LKKKQESFCR LQTEKETLFN
260 270 280 290 300
DSRNKIEELQ QRKEADLKAQ LARTQKLQQE LEAANQSLAE LRDQRQGERL
310 320 330 340 350
EHAAALRALQ DQIQTAKTQE LNMLREQTSE LASELQHRQA EYEELMGQKD
360 370 380 390 400
DLNSQLQESL RANSRLLEQL QEIGQEKEQL TQDLQEARKS AEKRKVMLDE
410 420 430 440 450
LAMETLQEKS QHKEELGAVR LRHEKELLGV RARYERELRE LHEDKKRQEE
460 470 480 490 500
ELRGQIREEK ARTRELENLQ HTVEELQAQV HSMDGAKGWF ERRLKEAEES
510 520 530 540 550
LQQQQQEQEE TLKLCREEHA AELKGKDEEL QNVREQLQQA QEERDGHVKT
560 570 580 590 600
ISNLKQEVKD TVDGQRILEK KGSAVLKDLK RQLHLERKRA DKLQERLQEI
610 620 630 640 650
LTNSKSRTGL EELVLSEMNS PSRTQTGDSS SVSSFSYREI LKEKESSAIP
660 670 680 690 700
ARSLSSSPQA QPPRPAELSD EEVAELFQRL AETQQEKWML EEKVKHLEVS
710 720 730 740 750
SASMAEDLCR KSAIIETYVM DSRIDVSVAA GHTDRSGLGS VLRDLVKPGD
760 770 780 790 800
ENLREMNKKL QNMLEEQLTK NMHLHKDMEV LSQEIVRLSK ECVGSPDPDL

EPGEAN
Length:806
Mass (Da):92,715
Last modified:March 1, 2002 - v1
Checksum:i766F7C5B44589020
GO

Sequence cautioni

The sequence CAA66184 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti4A → R in CAA66184 (Ref. 3) Curated1
Sequence conflicti502Q → L in BAE33608 (PubMed:16141072).Curated1
Sequence conflicti502Q → L in BAE43101 (PubMed:16141072).Curated1
Sequence conflicti502Q → L in BAE43133 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK156165 mRNA. Translation: BAE33608.1.
AK172623 mRNA. Translation: BAE43101.1.
AK172699 mRNA. Translation: BAE43133.1.
AL671995 Genomic DNA. No translation available.
X97571 mRNA. Translation: CAA66184.1. Different initiation.
AK173119 Transcribed RNA. Translation: BAD32397.1.
CCDSiCCDS40844.1.
RefSeqiNP_997553.1. NM_207670.2.
UniGeneiMm.194811.

Genome annotation databases

EnsembliENSMUST00000065932; ENSMUSP00000068789; ENSMUSG00000031153.
GeneIDi54645.
KEGGimmu:54645.
UCSCiuc009smo.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiGRAP1_MOUSE
AccessioniPrimary (citable) accession number: Q8VD04
Secondary accession number(s): A2AEW7
, O35693, Q3T9C3, Q69ZP9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2005
Last sequence update: March 1, 2002
Last modified: February 28, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome