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Protein

Hydroxyproline dehydrogenase

Gene

Prodh2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. Can also use proline as a substrate but with a very much lower efficiency. Does not react with other diastereomers of Hyp: trans-4-D-hydroxyproline and cis-4-L-hydroxyproline. Ubiquininone analogs such as menadione, duroquinone and ubiquinone-1 react more efficiently than oxygen as the terminal electron acceptor during catalysis.By similarity

Catalytic activityi

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol.By similarity
trans-4-hydroxy-L-proline + a quinone = (3R,5S)-1-pyrroline-3-hydroxy-5-carboxylate + a quinol.By similarity

Cofactori

FADBy similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processProline metabolism
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-389661. Glyoxylate metabolism and glycine degradation.
R-MMU-70688. Proline catabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyproline dehydrogenaseBy similarity (EC:1.5.5.-By similarity)
Short name:
HYPDHBy similarity
Alternative name(s):
Kidney and liver proline oxidase 1Curated
MmPOX1Curated
Probable proline dehydrogenase 2By similarity (EC:1.5.5.2By similarity)
Probable proline oxidase 2
Proline oxidase-like protein
Short name:
PO
Short name:
Proline oxidase
Gene namesi
Name:Prodh2Imported
Synonyms:HypdhBy similarity
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1929093. Prodh2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003086241 – 456Hydroxyproline dehydrogenaseAdd BLAST456

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei310N6-acetyllysineCombined sources1
Modified residuei320N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8VCZ9.
PaxDbiQ8VCZ9.
PRIDEiQ8VCZ9.

PTM databases

iPTMnetiQ8VCZ9.
PhosphoSitePlusiQ8VCZ9.
SwissPalmiQ8VCZ9.

Expressioni

Developmental stagei

Expressed in liver at E14. Expression level increases at P5 and decreases after P21.1 Publication

Inductioni

In liver, by TCF1 and HNF4A.1 Publication

Gene expression databases

BgeeiENSMUSG00000036892.
CleanExiMM_PRODH2.
ExpressionAtlasiQ8VCZ9. baseline and differential.
GenevisibleiQ8VCZ9. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8VCZ9. 1 interactor.
MINTiMINT-4119643.
STRINGi10090.ENSMUSP00000062214.

Structurei

3D structure databases

ProteinModelPortaliQ8VCZ9.
SMRiQ8VCZ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the proline oxidase family.Curated

Phylogenomic databases

eggNOGiKOG0186. Eukaryota.
COG0506. LUCA.
GeneTreeiENSGT00390000006265.
HOGENOMiHOG000233406.
HOVERGENiHBG108294.
InParanoidiQ8VCZ9.
KOiK11394.
OMAiIDAEESW.
OrthoDBiEOG091G09N3.
PhylomeDBiQ8VCZ9.
TreeFamiTF313544.

Family and domain databases

Gene3Di3.20.20.220. 1 hit.
InterProiView protein in InterPro
IPR029041. FAD-linked_oxidoreductase-like.
IPR002872. Proline_DH_dom.
IPR015659. Proline_oxidase.
PANTHERiPTHR13914. PTHR13914. 1 hit.
PfamiView protein in Pfam
PF01619. Pro_dh. 1 hit.
SUPFAMiSSF51730. SSF51730. 2 hits.

Sequencei

Sequence statusi: Complete.

Q8VCZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIWTRLPLYG PSKPSTGGWQ PLRFDGGAFH VKGTAELARA LLVLRLCAWP
60 70 80 90 100
PLVTHGLAFQ AWSQRLLGSR LSGALLRASI YGQFVAGETA EEVRNCVGQL
110 120 130 140 150
QALGLQPLLA VPTEEEPDST AKTSEVWYEE NLSAMLRCVD LSRALVDAHG
160 170 180 190 200
PARNSLMQLK VTALASTRLC KELSAWIQRP RGSSELSPER LAEAMDSGRN
210 220 230 240 250
LQLSCLSTEQ NQHLQASLSR LHRVAQHARA KCVRLLVDAE YTFINPALSL
260 270 280 290 300
LVAALAVRWN SPEEGGPWVW NTYQAYLKDT HQRLEQDAEA AHKAGLAFGV
310 320 330 340 350
KLVRGAYLDK ERSMTQLQGK EDCTQPDYEA TSRSYSRCLE LMLRCVSNHG
360 370 380 390 400
PPCHLMVASH NEESVRQATK RMWELGIPLD GPVCFGQLLG MCDHVSLALG
410 420 430 440 450
QAGYMVYKSI PYGCLEEVIP YLIRRAQENR SVLQGARREQ ALLSQELWRR

LLGRTA
Length:456
Mass (Da):50,723
Last modified:March 1, 2002 - v1
Checksum:iCD02C1474AADBD56
GO

Sequence cautioni

The sequence AAF21466 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAQ13907 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti337R → H in AAF21466 (Ref. 1) Curated1
Sequence conflicti416E → K in AAF21466 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80019 mRNA. Translation: AAF21466.1. Different initiation.
AF222851 mRNA. Translation: AAQ13907.1. Different initiation.
AK050141 mRNA. Translation: BAC34090.1.
BC018182 mRNA. Translation: AAH18182.1.
CCDSiCCDS21093.1.
RefSeqiNP_062419.2. NM_019546.5.
UniGeneiMm.270525.

Genome annotation databases

EnsembliENSMUST00000058280; ENSMUSP00000062214; ENSMUSG00000036892.
GeneIDi56189.
KEGGimmu:56189.
UCSCiuc009geo.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiHYPDH_MOUSE
AccessioniPrimary (citable) accession number: Q8VCZ9
Secondary accession number(s): Q2V058, Q9QX62
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: March 1, 2002
Last modified: August 30, 2017
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families