ID   SGSM3_MOUSE             Reviewed;         750 AA.
AC   Q8VCZ6; Q3TCB6;
DT   23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Small G protein signaling modulator 3;
DE   AltName: Full=RUN and TBC1 domain-containing protein 3;
GN   Name=Sgsm3; Synonyms=Cip85, Rutbc3;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH GJA1.
RC   TISSUE=Embryo;
RX   PubMed=15709751; DOI=10.1021/bi048306w;
RA   Lan Z., Kurata W.E., Martyn K.D., Jin C., Lau A.F.;
RT   "Novel rab GAP-like protein, CIP85, interacts with connexin43 and
RT   induces its degradation.";
RL   Biochemistry 44:2385-2396(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=NOD; TISSUE=Dendritic cell;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M.,
RA   Davis M.J., Wilming L.G., Aidinis V., Allen J.E.,
RA   Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L.,
RA   Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M.,
RA   Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R.,
RA   Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G.,
RA   di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G.,
RA   Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M.,
RA   Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N.,
RA   Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T.,
RA   Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H.,
RA   Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K.,
RA   Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J.,
RA   Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L.,
RA   Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K.,
RA   Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P.,
RA   Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O.,
RA   Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G.,
RA   Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M.,
RA   Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B.,
RA   Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K.,
RA   Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A.,
RA   Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K.,
RA   Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C.,
RA   Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J.,
RA   Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y.,
RA   Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T.,
RA   Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N.,
RA   Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N.,
RA   Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S.,
RA   Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J.,
RA   Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=FVB/N; TISSUE=Liver, Mammary tumor, and Salivary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [4]
RP   TISSUE SPECIFICITY.
RX   PubMed=17509819; DOI=10.1016/j.ygeno.2007.03.013;
RA   Yang H., Sasaki T., Minoshima S., Shimizu N.;
RT   "Identification of three novel proteins (SGSM1, 2, 3) which modulate
RT   small G protein (RAP and RAB)-mediated signaling pathway.";
RL   Genomics 90:249-260(2007).
RN   [5]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA   Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT   "Large-scale phosphorylation analysis of mouse liver.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN   [6]
RP   STRUCTURE BY NMR OF 483-549.
RG   RIKEN structural genomics initiative (RSGI);
RT   "Solution structure of the SH3 domain of mouse RUN and TBC1 domain
RT   containing 3.";
RL   Submitted (OCT-2007) to the PDB data bank.
CC   -!- FUNCTION: May play a cooperative role in NF2-mediated growth
CC       suppression of cells (By similarity).
CC   -!- SUBUNIT: Interacts with GJA1. Interaction with GJA1 induces its
CC       degradation. Interacts via its RUN domain with the C-terminal
CC       region of NF2. Interacts with RAB3A, RAB4A, RAB5A, RAB8A, RAB11A,
CC       RAP1A, RAP1B, RAP2A, RAP2B and PDCD6I. No interaction with RAB27A
CC       (By similarity).
CC   -!- INTERACTION:
CC       P08050:Gja1 (xeno); NbExp=1; IntAct=EBI-525155, EBI-476947;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- TISSUE SPECIFICITY: Widely expressed.
CC   -!- SIMILARITY: Belongs to the small G protein signaling modulator
CC       family.
CC   -!- SIMILARITY: Contains 1 Rab-GAP TBC domain.
CC   -!- SIMILARITY: Contains 1 RUN domain.
CC   -!- SIMILARITY: Contains 1 SH3 domain.
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DR   EMBL; AY382616; AAR89983.1; -; mRNA.
DR   EMBL; AK170806; BAE42041.1; ALT_INIT; mRNA.
DR   EMBL; BC018197; AAH18197.1; -; mRNA.
DR   EMBL; BC024122; AAH24122.1; -; mRNA.
DR   EMBL; BC025561; AAH25561.1; -; mRNA.
DR   IPI; IPI00311375; -.
DR   UniGene; Mm.274943; -.
DR   PDB; 2YUO; NMR; -; A=483-547.
DR   PDBsum; 2YUO; -.
DR   IntAct; Q8VCZ6; 1.
DR   PhosphoSite; Q8VCZ6; -.
DR   PRIDE; Q8VCZ6; -.
DR   Ensembl; ENSMUSG00000042303; Mus musculus.
DR   KEGG; mmu:105835; -.
DR   NMPDR; fig|10090.3.peg.30296; -.
DR   MGI; MGI:1916329; Sgsm3.
DR   HOVERGEN; Q8VCZ6; -.
DR   NextBio; 357910; -.
DR   ArrayExpress; Q8VCZ6; -.
DR   Bgee; Q8VCZ6; -.
DR   CleanEx; MM_SGSM3; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005921; C:gap junction; IDA:UniProtKB.
DR   GO; GO:0005097; F:Rab GTPase activator activity; IEA:InterPro.
DR   GO; GO:0017137; F:Rab GTPase binding; ISS:UniProtKB.
DR   GO; GO:0007050; P:cell cycle arrest; IEA:UniProtKB-KW.
DR   GO; GO:0045732; P:positive regulation of protein catabolic pr...; IDA:UniProtKB.
DR   GO; GO:0032486; P:Rap protein signal transduction; ISS:UniProtKB.
DR   GO; GO:0032313; P:regulation of Rab GTPase activity; IEA:InterPro.
DR   InterPro; IPR000195; RabGAP_TBC.
DR   InterPro; IPR004012; Run.
DR   InterPro; IPR001452; SH3_domain.
DR   Pfam; PF02759; RUN; 1.
DR   Pfam; PF00018; SH3_1; 1.
DR   Pfam; PF00566; TBC; 1.
DR   ProDom; PD000066; SH3; 1.
DR   SMART; SM00593; RUN; 1.
DR   SMART; SM00326; SH3; 1.
DR   SMART; SM00164; TBC; 1.
DR   PROSITE; PS50826; RUN; 1.
DR   PROSITE; PS50002; SH3; 1.
DR   PROSITE; PS50086; TBC_RABGAP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Coiled coil; Cytoplasm; Growth arrest;
KW   Phosphoprotein; SH3 domain.
FT   CHAIN         1    750       Small G protein signaling modulator 3.
FT                                /FTId=PRO_0000307811.
FT   DOMAIN      114    305       Rab-GAP TBC.
FT   DOMAIN      480    539       SH3.
FT   DOMAIN      555    718       RUN.
FT   COILED      415    439       Potential.
FT   MOD_RES     410    410       Phosphoserine.
FT   CONFLICT    622    622       D -> G (in Ref. 2; BAE42041).
FT   STRAND      483    489
FT   STRAND      506    511
FT   STRAND      514    522
FT   STRAND      525    530
FT   HELIX       531    533
FT   STRAND      534    537
SQ   SEQUENCE   750 AA;  85490 MW;  C0306BE346808B07 CRC64;
     MSGNHTPSAS GPFSALTPSI WPQEILAKYS QKEESSEQPE LCYDEFGFRV DKEGSEPGCS
     QMTGSPLVED PPQRLRWQAH LEFTHNHDVG DLTWDKIAVS LPRSEKLRSL VLAGIPHGMR
     PQLWMRLSGA LQKKKNSELS YREIIKNSSN DETIAAKQIE KDLLRTMPSN ACFANVNSIG
     VPRLRRVLRA LAWLYPEIGY CQGTGMVAAC LLLFLEEEDA FWMMCAIIED LLPASYFSTT
     LLGVQTDQRV LRHLIVQYLP RLDKLLQEHD IELSLITLHW FLTAFASVVH IRLLLRIWDL
     FFYEGSLVLF QTTLGMLRLK EEELIQSENS ASIFNTLSDI PAQMDDAELL LGEAMRLAGS
     LTDVAVETQR RKHLAYLIAD QGQTLGTGTT TNLSQVVRRR TQRRKSGITS LLFGEDDLEA
     LKAKNIKQTE LVADLREAIL RVARHFQCTD PKNCSVELTP DYSMESHQRD HENYVACLRS
     HRRRAKALLD FERHDDDELG FRKNDIITII SQKDEHCWVG ELNGLRGWFP AKFVEVLDER
     SKEYSIAGDD SVTEGVTDLV RGTLCPALKA LFEHGLKKPS LLGGACHPWL FIEEAAGREV
     ERDFDSVYSR LVLCKTYRLD EDGKVLTPEE LLYRAVQSVN VTHDAAHAQM DVKLRSLICV
     GLNEQVLHLW LEVLCSSLPT VEKWYQPWSF LRSPGWVQIK CELRVLCCFA FSLSQDWELP
     ARREEEKQPL KEGVQDMLVK HHLFSWDIDG
//