Q8VCZ6 (SGSM3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small G protein signaling modulator 3

Alternative name(s):
RUN and TBC1 domain-containing protein 3

Gene names

Name:	Sgsm3
Synonyms:	Cip85, Rutbc3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	750 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a cooperative role in NF2-mediated growth suppression of cells By similarity. Ref.4 UniProtKB Q96HU1
Subunit structure	Interacts with GJA1. Interaction with GJA1 induces its degradation. Interacts via its RUN domain with the C-terminal region of NF2. Interacts with RAB3A, RAB4A, RAB5A, RAB8A, RAB11A, RAP1A, RAP1B, RAP2A, RAP2B and PDCD6I. No interaction with RAB27A By similarity. Ref.1 UniProtKB Q96HU1
Subcellular location	Cytoplasm By similarity UniProtKB Q96HU1.
Tissue specificity	Widely expressed. Ref.4
Sequence similarities	Belongs to the small G protein signaling modulator family. Contains 1 Rab-GAP TBC domain. Contains 1 RUN domain. Contains 1 SH3 domain.
Sequence caution	The sequence BAE42041.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cell cycle Growth arrest
Cellular component	Cytoplasm
Domain	Coiled coil SH3 domain
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rap protein signal transduction Inferred from sequence or structural similarity. Source: UniProtKB cell cycle arrest Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of protein catabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell gap junction Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	Rab GTPase activator activity Inferred from sequence orthology PubMed 16923123. Source: MGI Rab GTPase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Gja1	P08050	4	EBI-525155,EBI-476947	From a different organism.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 750

750

Small G protein signaling modulator 3

PRO_0000307811

Regions

Domain

114 – 305

192

Rab-GAP TBC

Domain

480 – 539

60

SH3

Domain

555 – 718

164

RUN

Coiled coil

415 – 439

25

Potential

Amino acid modifications

Modified residue

410

1

Phosphoserine Ref.5

Experimental info

Sequence conflict

622

1

D → G in BAE42041. Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

750

Helix Strand Turn

Details...

Beta strand

483 – 489

7

Beta strand

506 – 511

6

Beta strand

514 – 522

9

Beta strand

525 – 530

6

Helix

531 – 533

3

Beta strand

534 – 537

4

Sequences

	Sequence		Length	Mass (Da)
	Q8VCZ6 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: C0306BE346808B07	FASTA	750	85,490
	10 20 30 40 50 60 MSGNHTPSAS GPFSALTPSI WPQEILAKYS QKEESSEQPE LCYDEFGFRV DKEGSEPGCS 70 80 90 100 110 120 QMTGSPLVED PPQRLRWQAH LEFTHNHDVG DLTWDKIAVS LPRSEKLRSL VLAGIPHGMR 130 140 150 160 170 180 PQLWMRLSGA LQKKKNSELS YREIIKNSSN DETIAAKQIE KDLLRTMPSN ACFANVNSIG 190 200 210 220 230 240 VPRLRRVLRA LAWLYPEIGY CQGTGMVAAC LLLFLEEEDA FWMMCAIIED LLPASYFSTT 250 260 270 280 290 300 LLGVQTDQRV LRHLIVQYLP RLDKLLQEHD IELSLITLHW FLTAFASVVH IRLLLRIWDL 310 320 330 340 350 360 FFYEGSLVLF QTTLGMLRLK EEELIQSENS ASIFNTLSDI PAQMDDAELL LGEAMRLAGS 370 380 390 400 410 420 LTDVAVETQR RKHLAYLIAD QGQTLGTGTT TNLSQVVRRR TQRRKSGITS LLFGEDDLEA 430 440 450 460 470 480 LKAKNIKQTE LVADLREAIL RVARHFQCTD PKNCSVELTP DYSMESHQRD HENYVACLRS 490 500 510 520 530 540 HRRRAKALLD FERHDDDELG FRKNDIITII SQKDEHCWVG ELNGLRGWFP AKFVEVLDER 550 560 570 580 590 600 SKEYSIAGDD SVTEGVTDLV RGTLCPALKA LFEHGLKKPS LLGGACHPWL FIEEAAGREV 610 620 630 640 650 660 ERDFDSVYSR LVLCKTYRLD EDGKVLTPEE LLYRAVQSVN VTHDAAHAQM DVKLRSLICV 670 680 690 700 710 720 GLNEQVLHLW LEVLCSSLPT VEKWYQPWSF LRSPGWVQIK CELRVLCCFA FSLSQDWELP 730 740 750 ARREEEKQPL KEGVQDMLVK HHLFSWDIDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel rab GAP-like protein, CIP85, interacts with connexin43 and induces its degradation." Lan Z., Kurata W.E., Martyn K.D., Jin C., Lau A.F. Biochemistry 44:2385-2396(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH GJA1. Tissue: Embryo.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Dendritic cell.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver, Mammary tumor and Salivary gland.
[4]	"Identification of three novel proteins (SGSM1, 2, 3) which modulate small G protein (RAP and RAB)-mediated signaling pathway." Yang H., Sasaki T., Minoshima S., Shimizu N. Genomics 90:249-260(2007) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-410, MASS SPECTROMETRY. Tissue: Liver.
[6]	"Solution structure of the SH3 domain of mouse RUN and TBC1 domain containing 3." RIKEN structural genomics initiative (RSGI) Submitted (OCT-2007) to the PDB data bank Cited for: STRUCTURE BY NMR OF 483-549.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AY382616 mRNA. Translation: AAR89983.1.
AK170806 mRNA. Translation: BAE42041.1. Different initiation.
BC018197 mRNA. Translation: AAH18197.1.
BC024122 mRNA. Translation: AAH24122.1.
BC025561 mRNA. Translation: AAH25561.1.

IPI

IPI00311375.

RefSeq

NP_598852.2. NM_134091.2.

UniGene

Mm.274943.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YUO	NMR	-	A	483-547	[»]

ProteinModelPortal

Q8VCZ6.

SMR

Q8VCZ6. Positions 92-349, 480-551.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q8VCZ6. 1 interaction.

MINT

MINT-1326710.

PTM databases

PhosphoSite

Q8VCZ6.

Proteomic databases

PaxDb

Q8VCZ6.

PRIDE

Q8VCZ6.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000139517; ENSMUSP00000122543; ENSMUSG00000042303.

GeneID

105835.

KEGG

mmu:105835.

UCSC

uc007wwb.2. mouse.

Organism-specific databases

CTD

27352.

MGI

MGI:1916329. Sgsm3.

Phylogenomic databases

eggNOG

COG5210.

GeneTree

ENSGT00700000104043.

HOGENOM

HOG000007875.

HOVERGEN

HBG108484.

InParanoid

Q8VCZ6.

OrthoDB

EOG4X97GJ.

Gene expression databases

ArrayExpress

Q8VCZ6.

Bgee

Q8VCZ6.

CleanEx

MM_SGSM3.

Genevestigator

Q8VCZ6.

Family and domain databases

InterPro

IPR000195. Rab-GTPase-TBC_dom.
IPR004012. Run.
IPR001452. SH3_domain.
[Graphical view]

Pfam

PF00566. RabGAP-TBC. 1 hit.
PF02759. RUN. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]

SMART

SM00593. RUN. 1 hit.
SM00326. SH3. 1 hit.
SM00164. TBC. 1 hit.
[Graphical view]

SUPFAM

SSF47923. RabGAP_TBC. 2 hits.
SSF50044. SH3. 1 hit.

PROSITE

PS50826. RUN. 1 hit.
PS50002. SH3. 1 hit.
PS50086. TBC_RABGAP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q8VCZ6.

NextBio

357910.

SOURCE

Search...

Entry information

Entry name

SGSM3_MOUSE

Accession

Primary (citable) accession number: Q8VCZ6
Secondary accession number(s): Q3TCB6

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 23, 2007
Last sequence update:	March 1, 2002
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program