Reviewed, UniProtKB/Swiss-Prot Q8VCX1 (AK1D1_MOUSE)

Names and origin

Protein names

Recommended name:
    3-oxo-5-beta-steroid 4-dehydrogenase
    EC=1.3.1.3
Alternative name(s):
    Delta(4)-3-ketosteroid 5-beta-reductase
    Delta(4)-3-oxosteroid 5-beta-reductase
    Aldo-keto reductase family 1 member D1

Gene names

Name:

Akr1d1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
Catalytic activity	5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH. 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Bile acid catabolism Lipid metabolism Steroid metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	bile acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	delta4-3-oxosteroid 5beta-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	3-oxo-5-beta-steroid 4-dehydrogenase		PRO_0000124670
Regions
	Nucleotide binding	168 – 169	2	NADP By similarity
	Nucleotide binding	219 – 223	5	NADP By similarity
	Nucleotide binding	272 – 282	11	NADP By similarity
Sites
	Active site	57	1	Proton donor By similarity
	Binding site	52	1	NADP By similarity
	Binding site	192	1	NADP By similarity
	Binding site	229	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VCX1-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1866A0DA4C5DDA98	FASTA	325	37,290
	10 20 30 40 50 60 MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH IDGAYVYHNE 70 80 90 100 110 120 HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA LERTLKALKL DYIDLYIIEL 130 140 150 160 170 180 PMAFKPGKEI YPRDENGRII YDKTNLCATW EALEACKDAG LVKSLGVSNF NRRQLELILN 190 200 210 220 230 240 KPGLKYKPVT NQVECHPYFT QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND 250 260 270 280 290 300 ELLTSLGKKY NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA 310 320 LNKNVRYVEL LMWSDHPEYP FHDEY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BC018333 mRNA. Translation: AAH18333.1.
IPI	IPI00122657.
RefSeq	NP_663339.1.
UniGene	Mm.262635
3D structure databases
HSSP	HSSP built from PDB template 1J96 based on UniProtKB P52895.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000038641. Mus musculus. [Contig view]
GeneID	208665.
KEGG	mmu:208665.
Organism-specific databases
MGI	MGI:2384785. Akr1d1.
Phylogenomic databases
HOGENOM	Q8VCX1.
HOVERGEN	Q8VCX1.
OMA	Q8VCX1. EACKDAS.
Enzyme and pathway databases
BRENDA	1.3.1.3. 244.
Gene expression databases
ArrayExpress	Q8VCX1.
Bgee	Q8VCX1.
CleanEx	MM_AKR1D1.
GermOnline	ENSMUSG00000038641. Mus musculus.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	372375.
SOURCE	Search...

Entry information

Entry name

AK1D1_MOUSE

Accession

Primary (citable) accession number: Q8VCX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2002
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)