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Protein

3-oxo-5-beta-steroid 4-dehydrogenase

Gene

Akr1d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.By similarity

Catalytic activityi

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.By similarity
17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26SubstrateBy similarity1
Binding sitei52NADPBy similarity1
Active sitei57Proton donorBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei88SubstrateBy similarity1
Binding sitei119SubstrateBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei192NADPBy similarity1
Binding sitei229SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 26NADPBy similarity5
Nucleotide bindingi168 – 169NADPBy similarity2
Nucleotide bindingi218 – 223NADPBy similarity6
Nucleotide bindingi272 – 282NADPBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase (EC:1.3.1.3)
Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase
Gene namesi
Name:Akr1d1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2384785. Akr1d1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246701 – 3253-oxo-5-beta-steroid 4-dehydrogenaseAdd BLAST325

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei228PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ8VCX1.
PaxDbiQ8VCX1.
PeptideAtlasiQ8VCX1.
PRIDEiQ8VCX1.

PTM databases

iPTMnetiQ8VCX1.
PhosphoSitePlusiQ8VCX1.
SwissPalmiQ8VCX1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000038641.
CleanExiMM_AKR1D1.
GenevisibleiQ8VCX1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8VCX1. 3 interactors.
MINTiMINT-1868906.
STRINGi10090.ENSMUSP00000048830.

Structurei

3D structure databases

ProteinModelPortaliQ8VCX1.
SMRiQ8VCX1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ8VCX1.
KOiK00251.
OMAiKTQAQIV.
OrthoDBiEOG091G0D69.
PhylomeDBiQ8VCX1.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH
60 70 80 90 100
IDGAYVYHNE HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA
110 120 130 140 150
LERTLKALKL DYIDLYIIEL PMAFKPGKEI YPRDENGRII YDKTNLCATW
160 170 180 190 200
EALEACKDAG LVKSLGVSNF NRRQLELILN KPGLKYKPVT NQVECHPYFT
210 220 230 240 250
QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND ELLTSLGKKY
260 270 280 290 300
NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA
310 320
LNKNVRYVEL LMWSDHPEYP FHDEY
Length:325
Mass (Da):37,290
Last modified:March 1, 2002 - v1
Checksum:i1866A0DA4C5DDA98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018333 mRNA. Translation: AAH18333.1.
CCDSiCCDS20007.1.
RefSeqiNP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGeneiMm.262635.

Genome annotation databases

EnsembliENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneIDi208665.
KEGGimmu:208665.
UCSCiuc009bjg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018333 mRNA. Translation: AAH18333.1.
CCDSiCCDS20007.1.
RefSeqiNP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGeneiMm.262635.

3D structure databases

ProteinModelPortaliQ8VCX1.
SMRiQ8VCX1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCX1. 3 interactors.
MINTiMINT-1868906.
STRINGi10090.ENSMUSP00000048830.

PTM databases

iPTMnetiQ8VCX1.
PhosphoSitePlusiQ8VCX1.
SwissPalmiQ8VCX1.

Proteomic databases

MaxQBiQ8VCX1.
PaxDbiQ8VCX1.
PeptideAtlasiQ8VCX1.
PRIDEiQ8VCX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneIDi208665.
KEGGimmu:208665.
UCSCiuc009bjg.2. mouse.

Organism-specific databases

CTDi6718.
MGIiMGI:2384785. Akr1d1.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ8VCX1.
KOiK00251.
OMAiKTQAQIV.
OrthoDBiEOG091G0D69.
PhylomeDBiQ8VCX1.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiR-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-MMU-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Miscellaneous databases

ChiTaRSiAkr1d1. mouse.
PROiQ8VCX1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038641.
CleanExiMM_AKR1D1.
GenevisibleiQ8VCX1. MM.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAK1D1_MOUSE
AccessioniPrimary (citable) accession number: Q8VCX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.