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Q8VCX1 (AK1D1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
3-oxo-5-beta-steroid 4-dehydrogenase

EC=1.3.1.3
Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase
Gene names
Name:Akr1d1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length325 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.

Catalytic activity

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.

17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the aldo/keto reductase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3253253-oxo-5-beta-steroid 4-dehydrogenase
PRO_0000124670

Regions

Nucleotide binding22 – 265NADP By similarity
Nucleotide binding168 – 1692NADP By similarity
Nucleotide binding218 – 2236NADP By similarity
Nucleotide binding272 – 28211NADP By similarity

Sites

Active site571Proton donor By similarity
Binding site261Substrate By similarity
Binding site521NADP By similarity
Binding site571Substrate By similarity
Binding site881Substrate By similarity
Binding site1191Substrate By similarity
Binding site1311Substrate By similarity
Binding site1921NADP By similarity
Binding site2291Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8VCX1 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 1866A0DA4C5DDA98

FASTA32537,290
        10         20         30         40         50         60 
MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH IDGAYVYHNE 

        70         80         90        100        110        120 
HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA LERTLKALKL DYIDLYIIEL 

       130        140        150        160        170        180 
PMAFKPGKEI YPRDENGRII YDKTNLCATW EALEACKDAG LVKSLGVSNF NRRQLELILN 

       190        200        210        220        230        240 
KPGLKYKPVT NQVECHPYFT QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND 

       250        260        270        280        290        300 
ELLTSLGKKY NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA 

       310        320 
LNKNVRYVEL LMWSDHPEYP FHDEY 

« Hide

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC018333 mRNA. Translation: AAH18333.1.
RefSeqNP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGeneMm.262635.

3D structure databases

ProteinModelPortalQ8VCX1.
SMRQ8VCX1. Positions 2-325.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActQ8VCX1. 3 interactions.
MINTMINT-1868906.
STRING10090.ENSMUSP00000048830.

PTM databases

PhosphoSiteQ8VCX1.

Proteomic databases

PaxDbQ8VCX1.
PRIDEQ8VCX1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneID208665.
KEGGmmu:208665.
UCSCuc009bjg.2. mouse.

Organism-specific databases

CTD6718.
MGIMGI:2384785. Akr1d1.

Phylogenomic databases

eggNOGCOG0656.
GeneTreeENSGT00550000074107.
HOGENOMHOG000250272.
HOVERGENHBG000020.
InParanoidQ8VCX1.
KOK00251.
OMAXVNISSP.
OrthoDBEOG70KGQF.
TreeFamTF106492.

Gene expression databases

ArrayExpressQ8VCX1.
BgeeQ8VCX1.
CleanExMM_AKR1D1.
GenevestigatorQ8VCX1.

Family and domain databases

Gene3D3.20.20.100. 1 hit.
InterProIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERPTHR11732. PTHR11732. 1 hit.
PfamPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFPIRSF000097. AKR. 1 hit.
PRINTSPR00069. ALDKETRDTASE.
SUPFAMSSF51430. SSF51430. 1 hit.
PROSITEPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAKR1D1. mouse.
NextBio372375.
PROQ8VCX1.
SOURCESearch...

Entry information

Entry nameAK1D1_MOUSE
AccessionPrimary (citable) accession number: Q8VCX1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2002
Last modified: March 19, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot