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Q8VCX1

- AK1D1_MOUSE

UniProt

Q8VCX1 - AK1D1_MOUSE

Protein

3-oxo-5-beta-steroid 4-dehydrogenase

Gene

Akr1d1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.

    Catalytic activityi

    5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.
    17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei26 – 261SubstrateBy similarity
    Binding sitei52 – 521NADPBy similarity
    Active sitei57 – 571Proton donorBy similarity
    Binding sitei57 – 571SubstrateBy similarity
    Binding sitei88 – 881SubstrateBy similarity
    Binding sitei119 – 1191SubstrateBy similarity
    Binding sitei131 – 1311SubstrateBy similarity
    Binding sitei192 – 1921NADPBy similarity
    Binding sitei229 – 2291SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi22 – 265NADPBy similarity
    Nucleotide bindingi168 – 1692NADPBy similarity
    Nucleotide bindingi218 – 2236NADPBy similarity
    Nucleotide bindingi272 – 28211NADPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. delta4-3-oxosteroid 5beta-reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. androgen metabolic process Source: Ensembl
    2. bile acid biosynthetic process Source: Ensembl
    3. bile acid catabolic process Source: UniProtKB-KW
    4. C21-steroid hormone metabolic process Source: Ensembl
    5. cholesterol catabolic process Source: Ensembl
    6. digestion Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

    Keywords - Ligandi

    NADP

    Enzyme and pathway databases

    ReactomeiREACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
    REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxo-5-beta-steroid 4-dehydrogenase (EC:1.3.1.3)
    Alternative name(s):
    Aldo-keto reductase family 1 member D1
    Delta(4)-3-ketosteroid 5-beta-reductase
    Delta(4)-3-oxosteroid 5-beta-reductase
    Gene namesi
    Name:Akr1d1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:2384785. Akr1d1.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytosol Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3253253-oxo-5-beta-steroid 4-dehydrogenasePRO_0000124670Add
    BLAST

    Proteomic databases

    MaxQBiQ8VCX1.
    PaxDbiQ8VCX1.
    PRIDEiQ8VCX1.

    PTM databases

    PhosphoSiteiQ8VCX1.

    Expressioni

    Gene expression databases

    BgeeiQ8VCX1.
    CleanExiMM_AKR1D1.
    GenevestigatoriQ8VCX1.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8VCX1. 3 interactions.
    MINTiMINT-1868906.
    STRINGi10090.ENSMUSP00000048830.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCX1.
    SMRiQ8VCX1. Positions 2-325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiCOG0656.
    GeneTreeiENSGT00750000117547.
    HOGENOMiHOG000250272.
    HOVERGENiHBG000020.
    InParanoidiQ8VCX1.
    KOiK00251.
    OMAiVYHNEHE.
    OrthoDBiEOG70KGQF.
    PhylomeDBiQ8VCX1.
    TreeFamiTF106492.

    Family and domain databases

    Gene3Di3.20.20.100. 1 hit.
    InterProiIPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view]
    PANTHERiPTHR11732. PTHR11732. 1 hit.
    PfamiPF00248. Aldo_ket_red. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000097. AKR. 1 hit.
    PRINTSiPR00069. ALDKETRDTASE.
    SUPFAMiSSF51430. SSF51430. 1 hit.
    PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VCX1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH    50
    IDGAYVYHNE HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA 100
    LERTLKALKL DYIDLYIIEL PMAFKPGKEI YPRDENGRII YDKTNLCATW 150
    EALEACKDAG LVKSLGVSNF NRRQLELILN KPGLKYKPVT NQVECHPYFT 200
    QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND ELLTSLGKKY 250
    NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA 300
    LNKNVRYVEL LMWSDHPEYP FHDEY 325
    Length:325
    Mass (Da):37,290
    Last modified:March 1, 2002 - v1
    Checksum:i1866A0DA4C5DDA98
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC018333 mRNA. Translation: AAH18333.1.
    CCDSiCCDS20007.1.
    RefSeqiNP_663339.1. NM_145364.2.
    XP_006505888.1. XM_006505825.1.
    UniGeneiMm.262635.

    Genome annotation databases

    EnsembliENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
    GeneIDi208665.
    KEGGimmu:208665.
    UCSCiuc009bjg.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC018333 mRNA. Translation: AAH18333.1 .
    CCDSi CCDS20007.1.
    RefSeqi NP_663339.1. NM_145364.2.
    XP_006505888.1. XM_006505825.1.
    UniGenei Mm.262635.

    3D structure databases

    ProteinModelPortali Q8VCX1.
    SMRi Q8VCX1. Positions 2-325.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VCX1. 3 interactions.
    MINTi MINT-1868906.
    STRINGi 10090.ENSMUSP00000048830.

    PTM databases

    PhosphoSitei Q8VCX1.

    Proteomic databases

    MaxQBi Q8VCX1.
    PaxDbi Q8VCX1.
    PRIDEi Q8VCX1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000040987 ; ENSMUSP00000048830 ; ENSMUSG00000038641 .
    GeneIDi 208665.
    KEGGi mmu:208665.
    UCSCi uc009bjg.2. mouse.

    Organism-specific databases

    CTDi 6718.
    MGIi MGI:2384785. Akr1d1.

    Phylogenomic databases

    eggNOGi COG0656.
    GeneTreei ENSGT00750000117547.
    HOGENOMi HOG000250272.
    HOVERGENi HBG000020.
    InParanoidi Q8VCX1.
    KOi K00251.
    OMAi VYHNEHE.
    OrthoDBi EOG70KGQF.
    PhylomeDBi Q8VCX1.
    TreeFami TF106492.

    Enzyme and pathway databases

    Reactomei REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
    REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

    Miscellaneous databases

    ChiTaRSi AKR1D1. mouse.
    NextBioi 372375.
    PROi Q8VCX1.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VCX1.
    CleanExi MM_AKR1D1.
    Genevestigatori Q8VCX1.

    Family and domain databases

    Gene3Di 3.20.20.100. 1 hit.
    InterProi IPR001395. Aldo/ket_red.
    IPR018170. Aldo/ket_reductase_CS.
    IPR020471. Aldo/keto_reductase_subgr.
    IPR023210. NADP_OxRdtase_dom.
    [Graphical view ]
    PANTHERi PTHR11732. PTHR11732. 1 hit.
    Pfami PF00248. Aldo_ket_red. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000097. AKR. 1 hit.
    PRINTSi PR00069. ALDKETRDTASE.
    SUPFAMi SSF51430. SSF51430. 1 hit.
    PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
    PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
    PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiAK1D1_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCX1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 7, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3