Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8VCX1

- AK1D1_MOUSE

UniProt

Q8VCX1 - AK1D1_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

3-oxo-5-beta-steroid 4-dehydrogenase

Gene

Akr1d1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.

Catalytic activityi

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.
17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstrateBy similarity
Binding sitei52 – 521NADPBy similarity
Active sitei57 – 571Proton donorBy similarity
Binding sitei57 – 571SubstrateBy similarity
Binding sitei88 – 881SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei192 – 1921NADPBy similarity
Binding sitei229 – 2291SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 265NADPBy similarity
Nucleotide bindingi168 – 1692NADPBy similarity
Nucleotide bindingi218 – 2236NADPBy similarity
Nucleotide bindingi272 – 28211NADPBy similarityAdd
BLAST

GO - Molecular functioni

  1. delta4-3-oxosteroid 5beta-reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. androgen metabolic process Source: Ensembl
  2. bile acid biosynthetic process Source: Ensembl
  3. bile acid catabolic process Source: UniProtKB-KW
  4. C21-steroid hormone metabolic process Source: Ensembl
  5. cholesterol catabolic process Source: Ensembl
  6. digestion Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase (EC:1.3.1.3)
Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase
Gene namesi
Name:Akr1d1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:2384785. Akr1d1.

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3253253-oxo-5-beta-steroid 4-dehydrogenasePRO_0000124670Add
BLAST

Proteomic databases

MaxQBiQ8VCX1.
PaxDbiQ8VCX1.
PRIDEiQ8VCX1.

PTM databases

PhosphoSiteiQ8VCX1.

Expressioni

Gene expression databases

BgeeiQ8VCX1.
CleanExiMM_AKR1D1.
ExpressionAtlasiQ8VCX1. baseline and differential.
GenevestigatoriQ8VCX1.

Interactioni

Protein-protein interaction databases

IntActiQ8VCX1. 3 interactions.
MINTiMINT-1868906.
STRINGi10090.ENSMUSP00000048830.

Structurei

3D structure databases

ProteinModelPortaliQ8VCX1.
SMRiQ8VCX1. Positions 2-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ8VCX1.
KOiK00251.
OMAiVYHNEHE.
OrthoDBiEOG70KGQF.
PhylomeDBiQ8VCX1.
TreeFamiTF106492.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCX1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH
60 70 80 90 100
IDGAYVYHNE HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA
110 120 130 140 150
LERTLKALKL DYIDLYIIEL PMAFKPGKEI YPRDENGRII YDKTNLCATW
160 170 180 190 200
EALEACKDAG LVKSLGVSNF NRRQLELILN KPGLKYKPVT NQVECHPYFT
210 220 230 240 250
QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND ELLTSLGKKY
260 270 280 290 300
NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA
310 320
LNKNVRYVEL LMWSDHPEYP FHDEY
Length:325
Mass (Da):37,290
Last modified:March 1, 2002 - v1
Checksum:i1866A0DA4C5DDA98
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC018333 mRNA. Translation: AAH18333.1.
CCDSiCCDS20007.1.
RefSeqiNP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGeneiMm.262635.

Genome annotation databases

EnsembliENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneIDi208665.
KEGGimmu:208665.
UCSCiuc009bjg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC018333 mRNA. Translation: AAH18333.1 .
CCDSi CCDS20007.1.
RefSeqi NP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGenei Mm.262635.

3D structure databases

ProteinModelPortali Q8VCX1.
SMRi Q8VCX1. Positions 2-325.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q8VCX1. 3 interactions.
MINTi MINT-1868906.
STRINGi 10090.ENSMUSP00000048830.

PTM databases

PhosphoSitei Q8VCX1.

Proteomic databases

MaxQBi Q8VCX1.
PaxDbi Q8VCX1.
PRIDEi Q8VCX1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000040987 ; ENSMUSP00000048830 ; ENSMUSG00000038641 .
GeneIDi 208665.
KEGGi mmu:208665.
UCSCi uc009bjg.2. mouse.

Organism-specific databases

CTDi 6718.
MGIi MGI:2384785. Akr1d1.

Phylogenomic databases

eggNOGi COG0656.
GeneTreei ENSGT00760000119041.
HOGENOMi HOG000250272.
HOVERGENi HBG000020.
InParanoidi Q8VCX1.
KOi K00251.
OMAi VYHNEHE.
OrthoDBi EOG70KGQF.
PhylomeDBi Q8VCX1.
TreeFami TF106492.

Enzyme and pathway databases

Reactomei REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_226440. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.

Miscellaneous databases

ChiTaRSi AKR1D1. mouse.
NextBioi 372375.
PROi Q8VCX1.
SOURCEi Search...

Gene expression databases

Bgeei Q8VCX1.
CleanExi MM_AKR1D1.
ExpressionAtlasi Q8VCX1. baseline and differential.
Genevestigatori Q8VCX1.

Family and domain databases

Gene3Di 3.20.20.100. 1 hit.
InterProi IPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view ]
PANTHERi PTHR11732. PTHR11732. 1 hit.
Pfami PF00248. Aldo_ket_red. 1 hit.
[Graphical view ]
PIRSFi PIRSF000097. AKR. 1 hit.
PRINTSi PR00069. ALDKETRDTASE.
SUPFAMi SSF51430. SSF51430. 1 hit.
PROSITEi PS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiAK1D1_MOUSE
AccessioniPrimary (citable) accession number: Q8VCX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2002
Last modified: October 29, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3