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Protein

3-oxo-5-beta-steroid 4-dehydrogenase

Gene

Akr1d1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.By similarity

Catalytic activityi

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.By similarity
17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstrateBy similarity
Binding sitei52 – 521NADPBy similarity
Active sitei57 – 571Proton donorBy similarity
Binding sitei57 – 571SubstrateBy similarity
Binding sitei88 – 881SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei192 – 1921NADPBy similarity
Binding sitei229 – 2291SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 265NADPBy similarity
Nucleotide bindingi168 – 1692NADPBy similarity
Nucleotide bindingi218 – 2236NADPBy similarity
Nucleotide bindingi272 – 28211NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiREACT_283453. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
REACT_292237. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_325766. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase (EC:1.3.1.3)
Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase
Gene namesi
Name:Akr1d1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2384785. Akr1d1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3253253-oxo-5-beta-steroid 4-dehydrogenasePRO_0000124670Add
BLAST

Proteomic databases

MaxQBiQ8VCX1.
PaxDbiQ8VCX1.
PRIDEiQ8VCX1.

PTM databases

PhosphoSiteiQ8VCX1.

Expressioni

Gene expression databases

BgeeiQ8VCX1.
CleanExiMM_AKR1D1.
ExpressionAtlasiQ8VCX1. baseline and differential.
GenevisibleiQ8VCX1. MM.

Interactioni

Protein-protein interaction databases

IntActiQ8VCX1. 3 interactions.
MINTiMINT-1868906.
STRINGi10090.ENSMUSP00000048830.

Structurei

3D structure databases

ProteinModelPortaliQ8VCX1.
SMRiQ8VCX1. Positions 2-325.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ8VCX1.
KOiK00251.
OMAiVYHNEHE.
OrthoDBiEOG70KGQF.
PhylomeDBiQ8VCX1.
TreeFamiTF106492.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCX1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH
60 70 80 90 100
IDGAYVYHNE HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA
110 120 130 140 150
LERTLKALKL DYIDLYIIEL PMAFKPGKEI YPRDENGRII YDKTNLCATW
160 170 180 190 200
EALEACKDAG LVKSLGVSNF NRRQLELILN KPGLKYKPVT NQVECHPYFT
210 220 230 240 250
QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND ELLTSLGKKY
260 270 280 290 300
NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA
310 320
LNKNVRYVEL LMWSDHPEYP FHDEY
Length:325
Mass (Da):37,290
Last modified:March 1, 2002 - v1
Checksum:i1866A0DA4C5DDA98
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018333 mRNA. Translation: AAH18333.1.
CCDSiCCDS20007.1.
RefSeqiNP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGeneiMm.262635.

Genome annotation databases

EnsembliENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneIDi208665.
KEGGimmu:208665.
UCSCiuc009bjg.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC018333 mRNA. Translation: AAH18333.1.
CCDSiCCDS20007.1.
RefSeqiNP_663339.1. NM_145364.2.
XP_006505888.1. XM_006505825.1.
UniGeneiMm.262635.

3D structure databases

ProteinModelPortaliQ8VCX1.
SMRiQ8VCX1. Positions 2-325.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCX1. 3 interactions.
MINTiMINT-1868906.
STRINGi10090.ENSMUSP00000048830.

PTM databases

PhosphoSiteiQ8VCX1.

Proteomic databases

MaxQBiQ8VCX1.
PaxDbiQ8VCX1.
PRIDEiQ8VCX1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneIDi208665.
KEGGimmu:208665.
UCSCiuc009bjg.2. mouse.

Organism-specific databases

CTDi6718.
MGIiMGI:2384785. Akr1d1.

Phylogenomic databases

eggNOGiCOG0656.
GeneTreeiENSGT00760000119041.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiQ8VCX1.
KOiK00251.
OMAiVYHNEHE.
OrthoDBiEOG70KGQF.
PhylomeDBiQ8VCX1.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiREACT_283453. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
REACT_292237. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_325766. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.

Miscellaneous databases

ChiTaRSiAkr1d1. mouse.
NextBioi372375.
PROiQ8VCX1.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCX1.
CleanExiMM_AKR1D1.
ExpressionAtlasiQ8VCX1. baseline and differential.
GenevisibleiQ8VCX1. MM.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase_subgr.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 1 hit.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.

Entry informationi

Entry nameiAK1D1_MOUSE
AccessioniPrimary (citable) accession number: Q8VCX1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: March 1, 2002
Last modified: June 24, 2015
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.