Q8VCX1 (AK1D1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase
EC=1.3.1.3

Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase

Gene names

Name:

Akr1d1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
Catalytic activity	5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH. 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Bile acid catabolism Lipid degradation Lipid metabolism Steroid metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	C21-steroid hormone metabolic process Inferred from electronic annotation. Source: Compara androgen metabolic process Inferred from electronic annotation. Source: Compara bile acid biosynthetic process Inferred from electronic annotation. Source: Compara bile acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW cholesterol catabolic process Inferred from electronic annotation. Source: Compara digestion Inferred from electronic annotation. Source: Compara
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara
Molecular_function	delta4-3-oxosteroid 5beta-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	3-oxo-5-beta-steroid 4-dehydrogenase		PRO_0000124670
Regions
	Nucleotide binding	168 – 169	2	NADP By similarity
	Nucleotide binding	219 – 223	5	NADP By similarity
	Nucleotide binding	272 – 282	11	NADP By similarity
Sites
	Active site	57	1	Proton donor By similarity
	Binding site	52	1	NADP By similarity
	Binding site	192	1	NADP By similarity
	Binding site	229	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VCX1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 1866A0DA4C5DDA98	FASTA	325	37,290
	10 20 30 40 50 60 MNLSAAHHQI SLSDGNNIPL IGLGTYSDPR PVPGKTYVAV KTAIDEGYRH IDGAYVYHNE 70 80 90 100 110 120 HEVGEAIREK IAEGKVKREE IFYCGKLWNT EHVPSMVLPA LERTLKALKL DYIDLYIIEL 130 140 150 160 170 180 PMAFKPGKEI YPRDENGRII YDKTNLCATW EALEACKDAG LVKSLGVSNF NRRQLELILN 190 200 210 220 230 240 KPGLKYKPVT NQVECHPYFT QTKLLKFCQQ HDIVIVAHSP LGTCRNPSWV NVSSPPLLND 250 260 270 280 290 300 ELLTSLGKKY NKTQAQIVLR FNIQRGIVVI PKSFTPERIK ENFQIFDFSL TEEEMKDIDA 310 320 LNKNVRYVEL LMWSDHPEYP FHDEY

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC018333 mRNA. Translation: AAH18333.1.
IPI	IPI00122657.
RefSeq	NP_663339.1. NM_145364.2.
UniGene	Mm.262635.
3D structure databases
ProteinModelPortal	Q8VCX1.
SMR	Q8VCX1. Positions 2-325.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000048830.
PTM databases
PhosphoSite	Q8VCX1.
Proteomic databases
PaxDb	Q8VCX1.
PRIDE	Q8VCX1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000040987; ENSMUSP00000048830; ENSMUSG00000038641.
GeneID	208665.
KEGG	mmu:208665.
UCSC	uc009bjg.2. mouse.
Organism-specific databases
CTD	6718.
MGI	MGI:2384785. Akr1d1.
Phylogenomic databases
eggNOG	COG0656.
GeneTree	ENSGT00550000074107.
HOGENOM	HOG000250272.
HOVERGEN	HBG000020.
InParanoid	Q8VCX1.
KO	K00251.
OMA	LNANIRT.
OrthoDB	EOG4Q2DG2.
Gene expression databases
ArrayExpress	Q8VCX1.
Bgee	Q8VCX1.
CleanEx	MM_AKR1D1.
Genevestigator	Q8VCX1.
GermOnline	ENSMUSG00000038641. Mus musculus.
Family and domain databases
Gene3D	3.20.20.100. 1 hit.
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
PANTHER	PTHR11732. PTHR11732. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	AKR1D1. mouse.
NextBio	372375.
SOURCE	Search...

Entry information

Entry name

AK1D1_MOUSE

Accession

Primary (citable) accession number: Q8VCX1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program