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Protein

Protein unc-93 homolog B1

Gene

Unc93b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in innate and adaptive immunity by regulating nucleotide-sensing Toll-like receptor (TLR) signaling. Required for the transport of a subset of TLRs (including TLR3, TLR7 and TLR9) from the endoplasmic reticulum to endolysosomes where they can engage pathogen nucleotides and activate signaling cascades. May play a role in autoreactive B-cells removal.3 Publications

GO - Molecular functioni

  • protein transporter activity Source: CACAO
  • Toll-like receptor binding Source: UniProtKB

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation Source: MGI
  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
  • cell morphogenesis Source: MGI
  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • intracellular protein transport Source: UniProtKB
  • positive regulation of interleukin-12 secretion Source: MGI
  • positive regulation of interleukin-6 secretion Source: MGI
  • toll-like receptor 3 signaling pathway Source: UniProtKB
  • toll-like receptor 7 signaling pathway Source: UniProtKB
  • toll-like receptor 9 signaling pathway Source: UniProtKB
  • toll-like receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Antiviral defense, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-93 homolog B1
Short name:
Unc-93B1
Gene namesi
Name:Unc93b1
Synonyms:Unc93b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1859307. Unc93b1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei64 – 8421HelicalSequence analysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence analysisAdd
BLAST
Transmembranei132 – 15221HelicalSequence analysisAdd
BLAST
Transmembranei160 – 18021HelicalSequence analysisAdd
BLAST
Transmembranei223 – 24321HelicalSequence analysisAdd
BLAST
Transmembranei285 – 30521HelicalSequence analysisAdd
BLAST
Transmembranei343 – 36321HelicalSequence analysisAdd
BLAST
Transmembranei378 – 39821HelicalSequence analysisAdd
BLAST
Transmembranei403 – 42321HelicalSequence analysisAdd
BLAST
Transmembranei428 – 44821HelicalSequence analysisAdd
BLAST
Transmembranei469 – 48921HelicalSequence analysisAdd
BLAST
Transmembranei495 – 51521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • early phagosome Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • endosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi34 – 341D → A: Increases the TLR7 response and decreases the TLR9 response. Enhances interaction with TLR7, TLR8 and TLR13 and TLR7 transport to endolysosomes in presence of single-stranded RNA. Decreases affinity for TLR9 and its transport to endolysosomes in presence of DNA. 1 Publication
Mutagenesisi36 – 361L → A: Increases the TLR7 response and decreases the TLR9 response.
Mutagenesisi412 – 4121H → R: Loss of function in TLR signaling. Impaired in its own transport from endoplasmic reticulum to endosome, lysosome and phagosome. Loss of the interaction with TLR3, TLR7, TLR9 and TLR13 and of the ability to transport them to endolysosome. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 598598Protein unc-93 homolog B1PRO_0000190041Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi251 – 2511N-linked (GlcNAc...)Sequence analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence analysis
Glycosylationi449 – 4491N-linked (GlcNAc...)Sequence analysis
Modified residuei547 – 5471PhosphoserineBy similarity
Modified residuei550 – 5501PhosphoserineBy similarity

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8VCW4.
MaxQBiQ8VCW4.
PaxDbiQ8VCW4.
PRIDEiQ8VCW4.

PTM databases

iPTMnetiQ8VCW4.
PhosphoSiteiQ8VCW4.

Expressioni

Inductioni

Upon interleukin-4 treatment in B-cells.1 Publication

Gene expression databases

BgeeiQ8VCW4.
CleanExiMM_UNC93B1.

Interactioni

Subunit structurei

Interacts with TLR3, TLR7, TLR8, TLR9 and TLR13 (probably via transmembrane domain).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CLPTM1O960052EBI-6116986,EBI-2873194From a different organism.
IRAK1P516172EBI-6116986,EBI-358664From a different organism.
MBOAT7Q96N662EBI-6116986,EBI-6116499From a different organism.
SURF4O152602EBI-6116986,EBI-1044848From a different organism.

GO - Molecular functioni

  • Toll-like receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8VCW4. 27 interactions.
STRINGi10090.ENSMUSP00000124272.

Structurei

3D structure databases

ProteinModelPortaliQ8VCW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the unc-93 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3097. Eukaryota.
ENOG410XTFP. LUCA.
HOGENOMiHOG000132956.
HOVERGENiHBG084638.
InParanoidiQ8VCW4.

Family and domain databases

InterProiIPR010291. Ion_channel_UNC-93.
[Graphical view]
PfamiPF05978. UNC-93. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VCW4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVEPPLYPV AGAAGPQGDE DRHGVPDGPE APLDELVGAY PNYNEEEEER
60 70 80 90 100
RYYRRKRLGV VKNVLAASTG VTLTYGVYLG LLQMQLILHY DETYREVKYG
110 120 130 140 150
NMGLPDIDSK MLMGINVTPI AALLYTPVLI RFFGTKWMMF LAVGIYALFV
160 170 180 190 200
STNYWERYYT LVPSAVALGM AIVPLWASMG NYITRMSQKY YEYSHYKEQD
210 220 230 240 250
EQGPQQRPPR GSHAPYLLVF QAIFYSFFHL SFACAQLPMI YFLNNYLYDL
260 270 280 290 300
NHTLINVQSC GTKSQGILNG FNKTVLRTLP RSKNLIVVES VLMAVAFLAM
310 320 330 340 350
LMVLGLCGAA YRPTEEIDLR SVGWGNIFQL PFKHVRDFRL RHLVPFFIYS
360 370 380 390 400
GFEVLFACTG FALGYGVCSM GLERLAYLLI AYSLGASASS VLGLLGLWLP
410 420 430 440 450
RSVPLVAGAG LHLLLTLSLF FWAPAPRVLQ HSWIFYFVAA LWGVGSALNK
460 470 480 490 500
TGLSTLLGIL YEDKERQDFI FTIYHWWQAV AIFVVYLGSS LPMKAKLAVL
510 520 530 540 550
LVTLVAAAAS YLWMEQKLQQ GLVPRQPRIP KPQHKVRGYR YLEEDNSDES
560 570 580 590
DMEGEQGQGD CAEDEAPQAG PLGAEPAGPC RKPCPYEQAL GGDGPEEQ
Length:598
Mass (Da):66,981
Last modified:July 27, 2011 - v2
Checksum:iE0E8AC1C3BBD9E10
GO
Isoform 2 (identifier: Q8VCW4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-292: Missing.

Note: No experimental confirmation available.
Show »
Length:306
Mass (Da):33,733
Checksum:iC470AC99734F2BA9
GO

Sequence cautioni

The sequence AAH25587.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti325 – 3262GN → RS in AAC36530 (PubMed:9798653).Curated
Sequence conflicti338 – 3381F → S in AAC36530 (PubMed:9798653).Curated
Sequence conflicti347 – 3471F → L in AAC36530 (PubMed:9798653).Curated
Sequence conflicti380 – 3801I → T in AAC36530 (PubMed:9798653).Curated
Sequence conflicti401 – 4033RSV → HSA in AAC36530 (PubMed:9798653).Curated
Sequence conflicti574 – 5741A → T in AAH18388 (PubMed:15489334).Curated
Sequence conflicti574 – 5741A → T in AAH23731 (PubMed:15489334).Curated
Sequence conflicti574 – 5741A → T in AAH25587 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 292292Missing in isoform 2. 1 PublicationVSP_014036Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK165060 mRNA. Translation: BAE38019.1.
BC018388 mRNA. Translation: AAH18388.1.
BC023731 mRNA. Translation: AAH23731.1.
BC025587 mRNA. Translation: AAH25587.1. Different initiation.
AJ422144 mRNA. Translation: CAD19524.1.
U89424 mRNA. Translation: AAC36530.1.
RefSeqiNP_001154900.1. NM_001161428.1.
NP_062322.2. NM_019449.2.
UniGeneiMm.262094.

Genome annotation databases

GeneIDi54445.
KEGGimmu:54445.
UCSCiuc008fxt.1. mouse. [Q8VCW4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK165060 mRNA. Translation: BAE38019.1.
BC018388 mRNA. Translation: AAH18388.1.
BC023731 mRNA. Translation: AAH23731.1.
BC025587 mRNA. Translation: AAH25587.1. Different initiation.
AJ422144 mRNA. Translation: CAD19524.1.
U89424 mRNA. Translation: AAC36530.1.
RefSeqiNP_001154900.1. NM_001161428.1.
NP_062322.2. NM_019449.2.
UniGeneiMm.262094.

3D structure databases

ProteinModelPortaliQ8VCW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCW4. 27 interactions.
STRINGi10090.ENSMUSP00000124272.

PTM databases

iPTMnetiQ8VCW4.
PhosphoSiteiQ8VCW4.

Proteomic databases

EPDiQ8VCW4.
MaxQBiQ8VCW4.
PaxDbiQ8VCW4.
PRIDEiQ8VCW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54445.
KEGGimmu:54445.
UCSCiuc008fxt.1. mouse. [Q8VCW4-1]

Organism-specific databases

CTDi81622.
MGIiMGI:1859307. Unc93b1.

Phylogenomic databases

eggNOGiKOG3097. Eukaryota.
ENOG410XTFP. LUCA.
HOGENOMiHOG000132956.
HOVERGENiHBG084638.
InParanoidiQ8VCW4.

Miscellaneous databases

NextBioi311310.
PROiQ8VCW4.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCW4.
CleanExiMM_UNC93B1.

Family and domain databases

InterProiIPR010291. Ion_channel_UNC-93.
[Graphical view]
PfamiPF05978. UNC-93. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J.
    Tissue: Oviduct.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Colon and Mammary tumor.
  3. "Cloning and characterization of mammalian homologs of unc-93 from Caenorhabditis elegans, a protein relevant for muscle contraction."
    Kollewe A.
    Thesis (2001), University of Hannover, Germany
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 264-315 (ISOFORM 1).
    Strain: C57BL/6 X CBA.
    Tissue: Spleen.
  4. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 317-434 (ISOFORM 1), INDUCTION.
    Strain: BALB/cJ.
    Tissue: Spleen.
  5. Cited for: FUNCTION, MUTAGENESIS OF HIS-412, SUBCELLULAR LOCATION.
  6. "The interaction between the ER membrane protein UNC93B and TLR3, 7, and 9 is crucial for TLR signaling."
    Brinkmann M.M., Spooner E., Hoebe K., Beutler B., Ploegh H.L., Kim Y.M.
    J. Cell Biol. 177:265-275(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, GLYCOSYLATION, INTERACTION WITH TLR3; TLR7; TLR9 AND TLR13.
  7. "UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes."
    Kim Y.M., Brinkmann M.M., Paquet M.E., Ploegh H.L.
    Nature 452:234-238(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  8. "Unc93B1 biases Toll-like receptor responses to nucleic acid in dendritic cells toward DNA- but against RNA-sensing."
    Fukui R., Saitoh S., Matsumoto F., Kozuka-Hata H., Oyama M., Tabeta K., Beutler B., Miyake K.
    J. Exp. Med. 206:1339-1350(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASP-34, INTERACTION WITH TLR7; TLR8 AND TLR13.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney, Lung and Spleen.

Entry informationi

Entry nameiUN93B_MOUSE
AccessioniPrimary (citable) accession number: Q8VCW4
Secondary accession number(s): O89077
, Q3TNR9, Q710D2, Q8CIK1, Q8R3D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 27, 2011
Last modified: March 16, 2016
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.