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Protein

Protein unc-93 homolog B1

Gene

Unc93b1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays an important role in innate and adaptive immunity by regulating nucleotide-sensing Toll-like receptor (TLR) signaling. Required for the transport of a subset of TLRs (including TLR3, TLR7 and TLR9) from the endoplasmic reticulum to endolysosomes where they can engage pathogen nucleotides and activate signaling cascades. May play a role in autoreactive B-cells removal.3 Publications

GO - Molecular functioni

  • protein transporter activity Source: CACAO
  • Toll-like receptor binding Source: UniProtKB

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • antigen processing and presentation Source: MGI
  • antigen processing and presentation of exogenous peptide antigen via MHC class II Source: MGI
  • cell morphogenesis Source: MGI
  • defense response to virus Source: UniProtKB-KW
  • innate immune response Source: UniProtKB-KW
  • intracellular protein transport Source: UniProtKB
  • positive regulation of interleukin-12 secretion Source: MGI
  • positive regulation of interleukin-6 secretion Source: MGI
  • toll-like receptor 3 signaling pathway Source: UniProtKB
  • toll-like receptor 7 signaling pathway Source: UniProtKB
  • toll-like receptor 9 signaling pathway Source: UniProtKB
  • toll-like receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Antiviral defense, Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-93 homolog B1
Short name:
Unc-93B1
Gene namesi
Name:Unc93b1
Synonyms:Unc93b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1859307. Unc93b1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei64 – 84HelicalSequence analysisAdd BLAST21
Transmembranei110 – 130HelicalSequence analysisAdd BLAST21
Transmembranei132 – 152HelicalSequence analysisAdd BLAST21
Transmembranei160 – 180HelicalSequence analysisAdd BLAST21
Transmembranei223 – 243HelicalSequence analysisAdd BLAST21
Transmembranei285 – 305HelicalSequence analysisAdd BLAST21
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Transmembranei378 – 398HelicalSequence analysisAdd BLAST21
Transmembranei403 – 423HelicalSequence analysisAdd BLAST21
Transmembranei428 – 448HelicalSequence analysisAdd BLAST21
Transmembranei469 – 489HelicalSequence analysisAdd BLAST21
Transmembranei495 – 515HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • early phagosome Source: UniProtKB
  • endoplasmic reticulum Source: UniProtKB
  • endoplasmic reticulum membrane Source: UniProtKB-SubCell
  • endosome Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endoplasmic reticulum, Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi34D → A: Increases the TLR7 response and decreases the TLR9 response. Enhances interaction with TLR7, TLR8 and TLR13 and TLR7 transport to endolysosomes in presence of single-stranded RNA. Decreases affinity for TLR9 and its transport to endolysosomes in presence of DNA. 1 Publication1
Mutagenesisi36L → A: Increases the TLR7 response and decreases the TLR9 response. 1
Mutagenesisi412H → R: Loss of function in TLR signaling. Impaired in its own transport from endoplasmic reticulum to endosome, lysosome and phagosome. Loss of the interaction with TLR3, TLR7, TLR9 and TLR13 and of the ability to transport them to endolysosome. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001900411 – 598Protein unc-93 homolog B1Add BLAST598

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi251N-linked (GlcNAc...)Sequence analysis1
Glycosylationi272N-linked (GlcNAc...)Sequence analysis1
Glycosylationi449N-linked (GlcNAc...)Sequence analysis1
Modified residuei547PhosphoserineBy similarity1
Modified residuei550PhosphoserineBy similarity1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8VCW4.
PaxDbiQ8VCW4.
PeptideAtlasiQ8VCW4.
PRIDEiQ8VCW4.

PTM databases

iPTMnetiQ8VCW4.
PhosphoSitePlusiQ8VCW4.

Expressioni

Inductioni

Upon interleukin-4 treatment in B-cells.1 Publication

Gene expression databases

BgeeiENSMUSG00000036908.
CleanExiMM_UNC93B1.

Interactioni

Subunit structurei

Interacts with TLR3, TLR7, TLR8, TLR9 and TLR13 (probably via transmembrane domain).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CLPTM1O960052EBI-6116986,EBI-2873194From a different organism.
IRAK1P516172EBI-6116986,EBI-358664From a different organism.
MBOAT7Q96N662EBI-6116986,EBI-6116499From a different organism.
SURF4O152602EBI-6116986,EBI-1044848From a different organism.

GO - Molecular functioni

  • Toll-like receptor binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ8VCW4. 27 interactors.
STRINGi10090.ENSMUSP00000124272.

Structurei

3D structure databases

ProteinModelPortaliQ8VCW4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the unc-93 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3097. Eukaryota.
ENOG410XTFP. LUCA.
HOGENOMiHOG000132956.
HOVERGENiHBG084638.
InParanoidiQ8VCW4.

Family and domain databases

InterProiIPR010291. Ion_channel_UNC-93.
[Graphical view]
PfamiPF05978. UNC-93. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VCW4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEVEPPLYPV AGAAGPQGDE DRHGVPDGPE APLDELVGAY PNYNEEEEER
60 70 80 90 100
RYYRRKRLGV VKNVLAASTG VTLTYGVYLG LLQMQLILHY DETYREVKYG
110 120 130 140 150
NMGLPDIDSK MLMGINVTPI AALLYTPVLI RFFGTKWMMF LAVGIYALFV
160 170 180 190 200
STNYWERYYT LVPSAVALGM AIVPLWASMG NYITRMSQKY YEYSHYKEQD
210 220 230 240 250
EQGPQQRPPR GSHAPYLLVF QAIFYSFFHL SFACAQLPMI YFLNNYLYDL
260 270 280 290 300
NHTLINVQSC GTKSQGILNG FNKTVLRTLP RSKNLIVVES VLMAVAFLAM
310 320 330 340 350
LMVLGLCGAA YRPTEEIDLR SVGWGNIFQL PFKHVRDFRL RHLVPFFIYS
360 370 380 390 400
GFEVLFACTG FALGYGVCSM GLERLAYLLI AYSLGASASS VLGLLGLWLP
410 420 430 440 450
RSVPLVAGAG LHLLLTLSLF FWAPAPRVLQ HSWIFYFVAA LWGVGSALNK
460 470 480 490 500
TGLSTLLGIL YEDKERQDFI FTIYHWWQAV AIFVVYLGSS LPMKAKLAVL
510 520 530 540 550
LVTLVAAAAS YLWMEQKLQQ GLVPRQPRIP KPQHKVRGYR YLEEDNSDES
560 570 580 590
DMEGEQGQGD CAEDEAPQAG PLGAEPAGPC RKPCPYEQAL GGDGPEEQ
Length:598
Mass (Da):66,981
Last modified:July 27, 2011 - v2
Checksum:iE0E8AC1C3BBD9E10
GO
Isoform 2 (identifier: Q8VCW4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-292: Missing.

Note: No experimental confirmation available.
Show »
Length:306
Mass (Da):33,733
Checksum:iC470AC99734F2BA9
GO

Sequence cautioni

The sequence AAH25587 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti325 – 326GN → RS in AAC36530 (PubMed:9798653).Curated2
Sequence conflicti338F → S in AAC36530 (PubMed:9798653).Curated1
Sequence conflicti347F → L in AAC36530 (PubMed:9798653).Curated1
Sequence conflicti380I → T in AAC36530 (PubMed:9798653).Curated1
Sequence conflicti401 – 403RSV → HSA in AAC36530 (PubMed:9798653).Curated3
Sequence conflicti574A → T in AAH18388 (PubMed:15489334).Curated1
Sequence conflicti574A → T in AAH23731 (PubMed:15489334).Curated1
Sequence conflicti574A → T in AAH25587 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0140361 – 292Missing in isoform 2. 1 PublicationAdd BLAST292

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK165060 mRNA. Translation: BAE38019.1.
BC018388 mRNA. Translation: AAH18388.1.
BC023731 mRNA. Translation: AAH23731.1.
BC025587 mRNA. Translation: AAH25587.1. Different initiation.
AJ422144 mRNA. Translation: CAD19524.1.
U89424 mRNA. Translation: AAC36530.1.
RefSeqiNP_001154900.1. NM_001161428.1.
NP_062322.2. NM_019449.2.
UniGeneiMm.262094.

Genome annotation databases

GeneIDi54445.
KEGGimmu:54445.
UCSCiuc008fxt.1. mouse. [Q8VCW4-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK165060 mRNA. Translation: BAE38019.1.
BC018388 mRNA. Translation: AAH18388.1.
BC023731 mRNA. Translation: AAH23731.1.
BC025587 mRNA. Translation: AAH25587.1. Different initiation.
AJ422144 mRNA. Translation: CAD19524.1.
U89424 mRNA. Translation: AAC36530.1.
RefSeqiNP_001154900.1. NM_001161428.1.
NP_062322.2. NM_019449.2.
UniGeneiMm.262094.

3D structure databases

ProteinModelPortaliQ8VCW4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCW4. 27 interactors.
STRINGi10090.ENSMUSP00000124272.

PTM databases

iPTMnetiQ8VCW4.
PhosphoSitePlusiQ8VCW4.

Proteomic databases

EPDiQ8VCW4.
PaxDbiQ8VCW4.
PeptideAtlasiQ8VCW4.
PRIDEiQ8VCW4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi54445.
KEGGimmu:54445.
UCSCiuc008fxt.1. mouse. [Q8VCW4-1]

Organism-specific databases

CTDi81622.
MGIiMGI:1859307. Unc93b1.

Phylogenomic databases

eggNOGiKOG3097. Eukaryota.
ENOG410XTFP. LUCA.
HOGENOMiHOG000132956.
HOVERGENiHBG084638.
InParanoidiQ8VCW4.

Miscellaneous databases

PROiQ8VCW4.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000036908.
CleanExiMM_UNC93B1.

Family and domain databases

InterProiIPR010291. Ion_channel_UNC-93.
[Graphical view]
PfamiPF05978. UNC-93. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUN93B_MOUSE
AccessioniPrimary (citable) accession number: Q8VCW4
Secondary accession number(s): O89077
, Q3TNR9, Q710D2, Q8CIK1, Q8R3D0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.