Q8VCT9 (TESK2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity testis-specific protein kinase 2
EC=2.7.12.1

Alternative name(s):
Testicular protein kinase 2

Gene names

Name:

Tesk2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	570 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity. Manganese By similarity.
Enzyme regulation	Activated by autophosphorylation on Ser-219 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	actin cytoskeleton organization Inferred from sequence or structural similarity. Source: UniProtKB focal adhesion assembly Inferred from sequence or structural similarity. Source: UniProtKB spermatogenesis Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB protein serine/threonine/tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 570	570	Dual specificity testis-specific protein kinase 2		PRO_0000086750
Regions
	Domain	58 – 313	256	Protein kinase
	Nucleotide binding	64 – 72	9	ATP By similarity
Sites
	Active site	176	1	Proton acceptor By similarity
	Binding site	87	1	ATP By similarity
Amino acid modifications
	Modified residue	219	1	Phosphoserine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VCT9 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 7B5A48E16655EAD1	FASTA	570	63,470
	10 20 30 40 50 60 MDRSKRNSIA GFPPRVERLE EFEGGGGGDG NTVQVGRVSS SSYRAIISAF SRLTSLDDFT 70 80 90 100 110 120 REKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANLLK EMQLMNRLSH PNILRFMGVC 130 140 150 160 170 180 VHQGQLHALT EYINSGNLEQ LLDSDLYLPW TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK 190 200 210 220 230 240 NCLIKRDENG YSAVVADFGL AEKIPDASIG REKLAVVGSP FWMAPEVLRD EPYNEKADVF 250 260 270 280 290 300 SYGIILCEII ARIQADPDYL PRTENFGLDY DAFQNMVGDC PSDFLQLTFN CCNMDPKLRP 310 320 330 340 350 360 SFEEIGKTLK EIMSRLPEEE LERDRKLQPT AKGPLEKVPG GKRLSSLDDK IPHKSPRPRR 370 380 390 400 410 420 TIWLSRSQSD IFSHKPPRTV SVLDPYYQPR DGATHTPKVN PFSARQDLKG GKVKFFDLPS 430 440 450 460 470 480 KSVISLVFDL DAPGPGSTTL ADCQEPLAMS SRRWRSLPGS PEFLHQACPF MGCEESLSDG 490 500 510 520 530 540 PPPRLSSLKY GVREIPPFRT SALSAASGHE AMDCSNPQEE NGFGPRLKGT SLCTGAASEE 550 560 570 MEVEEERPRR ASVYFSISGI SLQTQAKQDG

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC019149 mRNA. Translation: AAH19149.1. BC029766 mRNA. Translation: AAH29766.1.
IPI	IPI00122542.
RefSeq	NP_666263.3. NM_146151.4.
UniGene	Mm.482431.
3D structure databases
ProteinModelPortal	Q8VCT9.
SMR	Q8VCT9. Positions 56-315.
ModBase	Search...
Protein-protein interaction databases
STRING	Q8VCT9.
PTM databases
PhosphoSite	Q8VCT9.
Proteomic databases
PRIDE	Q8VCT9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000045542; ENSMUSP00000041009; ENSMUSG00000033985.
GeneID	230661.
KEGG	mmu:230661.
Organism-specific databases
CTD	10420.
MGI	MGI:2385204. Tesk2.
Phylogenomic databases
GeneTree	ENSGT00530000063025.
HOGENOM	HBG446747.
HOVERGEN	HBG058204.
InParanoid	Q8VCT9.
OMA	SPEFLHQ.
OrthoDB	EOG4NZTSW.
PhylomeDB	Q8VCT9.
Gene expression databases
ArrayExpress	Q8VCT9.
Bgee	Q8VCT9.
CleanEx	MM_TESK2.
Genevestigator	Q8VCT9.
GermOnline	ENSMUSG00000033985. Mus musculus.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase. IPR008266. Tyr_kinase_AS. [Graphical view]
KO	K08842.
Pfam	PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

TESK2_MOUSE

Accession

Primary (citable) accession number: Q8VCT9

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 8, 2002
Last sequence update:	March 1, 2002
Last modified:	November 16, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program