Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dual specificity testis-specific protein kinase 2

Gene

Tesk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by autophosphorylation on Ser-219.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei87 – 871ATPPROSITE-ProRule annotation
Active sitei176 – 1761Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi64 – 729ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine/tyrosine kinase activity Source: UniProtKB-EC
  4. protein serine/threonine kinase activity Source: UniProtKB
  5. protein tyrosine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. actin cytoskeleton organization Source: UniProtKB
  2. focal adhesion assembly Source: UniProtKB
  3. protein phosphorylation Source: UniProtKB
  4. spermatogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity testis-specific protein kinase 2 (EC:2.7.12.1)
Alternative name(s):
Testicular protein kinase 2
Gene namesi
Name:Tesk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:2385204. Tesk2.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleoplasm Source: MGI
  3. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 570570Dual specificity testis-specific protein kinase 2PRO_0000086750Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei219 – 2191Phosphoserine; by autocatalysisBy similarity
Modified residuei456 – 4561PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ8VCT9.
PRIDEiQ8VCT9.

PTM databases

PhosphoSiteiQ8VCT9.

Expressioni

Gene expression databases

BgeeiQ8VCT9.
CleanExiMM_TESK2.
ExpressionAtlasiQ8VCT9. baseline and differential.
GenevestigatoriQ8VCT9.

Structurei

3D structure databases

ProteinModelPortaliQ8VCT9.
SMRiQ8VCT9. Positions 62-378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 313256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiQ8VCT9.
KOiK08842.
OMAiNVLDPYY.
OrthoDBiEOG7V7664.
PhylomeDBiQ8VCT9.
TreeFamiTF318014.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCT9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDRSKRNSIA GFPPRVERLE EFEGGGGGDG NTVQVGRVSS SSYRAIISAF
60 70 80 90 100
SRLTSLDDFT REKIGSGFFS EVFKVRHRAS GQVMALKMNT LSSNRANLLK
110 120 130 140 150
EMQLMNRLSH PNILRFMGVC VHQGQLHALT EYINSGNLEQ LLDSDLYLPW
160 170 180 190 200
TVRVKLAYDI AVGLSYLHFK GIFHRDLTSK NCLIKRDENG YSAVVADFGL
210 220 230 240 250
AEKIPDASIG REKLAVVGSP FWMAPEVLRD EPYNEKADVF SYGIILCEII
260 270 280 290 300
ARIQADPDYL PRTENFGLDY DAFQNMVGDC PSDFLQLTFN CCNMDPKLRP
310 320 330 340 350
SFEEIGKTLK EIMSRLPEEE LERDRKLQPT AKGPLEKVPG GKRLSSLDDK
360 370 380 390 400
IPHKSPRPRR TIWLSRSQSD IFSHKPPRTV SVLDPYYQPR DGATHTPKVN
410 420 430 440 450
PFSARQDLKG GKVKFFDLPS KSVISLVFDL DAPGPGSTTL ADCQEPLAMS
460 470 480 490 500
SRRWRSLPGS PEFLHQACPF MGCEESLSDG PPPRLSSLKY GVREIPPFRT
510 520 530 540 550
SALSAASGHE AMDCSNPQEE NGFGPRLKGT SLCTGAASEE MEVEEERPRR
560 570
ASVYFSISGI SLQTQAKQDG
Length:570
Mass (Da):63,470
Last modified:March 1, 2002 - v1
Checksum:i7B5A48E16655EAD1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019149 mRNA. Translation: AAH19149.1.
BC029766 mRNA. Translation: AAH29766.1.
CCDSiCCDS18516.1.
RefSeqiNP_666263.3. NM_146151.4.
UniGeneiMm.482431.

Genome annotation databases

EnsembliENSMUST00000045542; ENSMUSP00000041009; ENSMUSG00000033985.
GeneIDi230661.
KEGGimmu:230661.
UCSCiuc008uhj.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC019149 mRNA. Translation: AAH19149.1.
BC029766 mRNA. Translation: AAH29766.1.
CCDSiCCDS18516.1.
RefSeqiNP_666263.3. NM_146151.4.
UniGeneiMm.482431.

3D structure databases

ProteinModelPortaliQ8VCT9.
SMRiQ8VCT9. Positions 62-378.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8VCT9.

Proteomic databases

PaxDbiQ8VCT9.
PRIDEiQ8VCT9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045542; ENSMUSP00000041009; ENSMUSG00000033985.
GeneIDi230661.
KEGGimmu:230661.
UCSCiuc008uhj.2. mouse.

Organism-specific databases

CTDi10420.
MGIiMGI:2385204. Tesk2.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00530000063025.
HOGENOMiHOG000231415.
HOVERGENiHBG058204.
InParanoidiQ8VCT9.
KOiK08842.
OMAiNVLDPYY.
OrthoDBiEOG7V7664.
PhylomeDBiQ8VCT9.
TreeFamiTF318014.

Miscellaneous databases

ChiTaRSiTesk2. mouse.
NextBioi380033.
PROiQ8VCT9.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCT9.
CleanExiMM_TESK2.
ExpressionAtlasiQ8VCT9. baseline and differential.
GenevestigatoriQ8VCT9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008266. Tyr_kinase_AS.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
PRINTSiPR00109. TYRKINASE.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiTESK2_MOUSE
AccessioniPrimary (citable) accession number: Q8VCT9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: March 4, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.