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Protein

Aminopeptidase B

Gene

Rnpep

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg0-Leu-enkephalin, Arg0-Met-enkephalin and Arg(-1)-Lys0-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity).By similarity

Catalytic activityi

Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi325Zinc; catalyticPROSITE-ProRule annotation1
Active sitei326Proton acceptorPROSITE-ProRule annotation1
Metal bindingi329Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi348Zinc; catalyticPROSITE-ProRule annotation1
Sitei414Transition state stabilizerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiM01.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase B (EC:3.4.11.6)
Short name:
AP-B
Alternative name(s):
Arginine aminopeptidase
Arginyl aminopeptidase
Cytosol aminopeptidase IV
Gene namesi
Name:Rnpep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2384902. Rnpep.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2836.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000950891 – 650Aminopeptidase BAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei446N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8VCT3.
MaxQBiQ8VCT3.
PaxDbiQ8VCT3.
PeptideAtlasiQ8VCT3.
PRIDEiQ8VCT3.

PTM databases

iPTMnetiQ8VCT3.
PhosphoSitePlusiQ8VCT3.
SwissPalmiQ8VCT3.

Expressioni

Gene expression databases

BgeeiENSMUSG00000041926.
CleanExiMM_RNPEP.
ExpressionAtlasiQ8VCT3. baseline and differential.
GenevisibleiQ8VCT3. MM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi229640. 2 interactors.
IntActiQ8VCT3. 2 interactors.
MINTiMINT-4087642.
STRINGi10090.ENSMUSP00000076564.

Chemistry databases

BindingDBiQ8VCT3.

Structurei

3D structure databases

ProteinModelPortaliQ8VCT3.
SMRiQ8VCT3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni298 – 302Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiKOG1047. Eukaryota.
COG0308. LUCA.
GeneTreeiENSGT00530000063003.
HOGENOMiHOG000293296.
HOVERGENiHBG001274.
InParanoidiQ8VCT3.
KOiK01260.
OMAiEGPGVCW.
OrthoDBiEOG091G02UX.
TreeFamiTF300758.

Family and domain databases

CDDicd09599. M1_LTA4H. 1 hit.
InterProiView protein in InterPro
IPR016024. ARM-type_fold.
IPR034015. M1_LTA4H.
IPR015571. Pept_M1_aminopeptidase-B.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF231. PTHR11533:SF231. 1 hit.
PfamiView protein in Pfam
PF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
PRINTSiPR00756. ALADIPTASE.
SMARTiView protein in SMART
SM01263. Leuk-A4-hydro_C. 1 hit.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiView protein in PROSITE
PS00142. ZINC_PROTEASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8VCT3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MESGGPGNYS AAARRPLHSA QAVDVASASS FRAFEILHLH LDLRAEFGPP
60 70 80 90 100
GPGPGSRGLS GTATLELRCL LPEGASELRL DSHSCLEVTA ATLRRGQPGD
110 120 130 140 150
QQAPAEPVPF HTQPFSHYGQ ALCVAFRQPC GAADRFELEL TYRVGEGPGV
160 170 180 190 200
CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKCT YSALIEVPDG
210 220 230 240 250
FTAVMSADTW EKRGPNKFFF QMSHPIPSYL IALAIGDLAS AEVGPRSRVW
260 270 280 290 300
AEPCLIEAAK EEYSGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM
310 320 330 340 350
ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF
360 370 380 390 400
TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMNVSGEEN PLNKLRVKIE
410 420 430 440 450
PGVDPDDTYN ETPYEKGYCF VSYLAHLVGD QDQFDKFLKA YVDEFKFQSI
460 470 480 490 500
LAEDFLEFYL EYFPELKKKG VDSIPGFEFD RWLNTPGWPP YLPDLSPGDS
510 520 530 540 550
LMKPAEELAE LWVTSEPDMQ AIEAVAISTW KTYQLVYFLD KILQKSPLPP
560 570 580 590 600
GNVKKLGETY PKISNAQNAE LRLRWGQIIL KNDYQEEFQK VKDFLQSQGK
610 620 630 640 650
QKYTLPLYHA MMGGSEMART LAKDTFAATA SQLHSNVVNY VQQILAPKDS
Length:650
Mass (Da):72,416
Last modified:July 27, 2011 - v2
Checksum:i95138D47E57E3C61
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11A → G in AAH19200 (PubMed:15489334).Curated1
Sequence conflicti127R → P in AAH19200 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK159195 mRNA. Translation: BAE34890.1.
AK159445 mRNA. Translation: BAE35089.1.
AK159625 mRNA. Translation: BAE35239.1.
CH466520 Genomic DNA. Translation: EDL39584.1.
BC019200 mRNA. Translation: AAH19200.1.
CCDSiCCDS15317.1.
RefSeqiNP_001153096.1. NM_001159624.1.
NP_663392.2. NM_145417.3.
UniGeneiMm.291048.

Genome annotation databases

EnsembliENSMUST00000077340; ENSMUSP00000076564; ENSMUSG00000041926.
GeneIDi215615.
KEGGimmu:215615.
UCSCiuc007csy.2. mouse.

Similar proteinsi

Entry informationi

Entry nameiAMPB_MOUSE
AccessioniPrimary (citable) accession number: Q8VCT3
Secondary accession number(s): Q3TX27
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 27, 2011
Last modified: August 30, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families