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Q8VCT3

- AMPB_MOUSE

UniProt

Q8VCT3 - AMPB_MOUSE

Protein

Aminopeptidase B

Gene

Rnpep

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) By similarity.By similarity

    Catalytic activityi

    Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi325 – 3251Zinc; catalyticPROSITE-ProRule annotation
    Active sitei326 – 3261Proton acceptorPROSITE-ProRule annotation
    Metal bindingi329 – 3291Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi348 – 3481Zinc; catalyticPROSITE-ProRule annotation
    Sitei414 – 4141Transition state stabilizerBy similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. leukotriene biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM01.014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aminopeptidase B (EC:3.4.11.6)
    Short name:
    AP-B
    Alternative name(s):
    Arginine aminopeptidase
    Arginyl aminopeptidase
    Cytosol aminopeptidase IV
    Gene namesi
    Name:Rnpep
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:2384902. Rnpep.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: InterPro
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 650649Aminopeptidase BPRO_0000095089Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei446 – 4461N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiQ8VCT3.
    PaxDbiQ8VCT3.
    PRIDEiQ8VCT3.

    PTM databases

    PhosphoSiteiQ8VCT3.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8VCT3.
    BgeeiQ8VCT3.
    CleanExiMM_RNPEP.
    GenevestigatoriQ8VCT3.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    BioGridi229640. 1 interaction.
    IntActiQ8VCT3. 2 interactions.
    MINTiMINT-4087642.
    STRINGi10090.ENSMUSP00000076564.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCT3.
    SMRiQ8VCT3. Positions 61-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni298 – 3025Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the peptidase M1 family.Curated

    Phylogenomic databases

    eggNOGiCOG0308.
    GeneTreeiENSGT00530000063003.
    HOGENOMiHOG000293296.
    HOVERGENiHBG001274.
    InParanoidiQ3TX27.
    KOiK01260.
    OMAiPPGNVKK.
    OrthoDBiEOG7SJD42.
    TreeFamiTF300758.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR015571. Pept_M1_aminopeptidase-B.
    IPR001930. Peptidase_M1.
    IPR015211. Peptidase_M1_C.
    IPR014782. Peptidase_M1_N.
    [Graphical view]
    PANTHERiPTHR11533. PTHR11533. 1 hit.
    PTHR11533:SF153. PTHR11533:SF153. 1 hit.
    PfamiPF09127. Leuk-A4-hydro_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view]
    PRINTSiPR00756. ALADIPTASE.
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VCT3-1 [UniParc]FASTAAdd to Basket

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    MESGGPGNYS AAARRPLHSA QAVDVASASS FRAFEILHLH LDLRAEFGPP    50
    GPGPGSRGLS GTATLELRCL LPEGASELRL DSHSCLEVTA ATLRRGQPGD 100
    QQAPAEPVPF HTQPFSHYGQ ALCVAFRQPC GAADRFELEL TYRVGEGPGV 150
    CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKCT YSALIEVPDG 200
    FTAVMSADTW EKRGPNKFFF QMSHPIPSYL IALAIGDLAS AEVGPRSRVW 250
    AEPCLIEAAK EEYSGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM 300
    ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF 350
    TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMNVSGEEN PLNKLRVKIE 400
    PGVDPDDTYN ETPYEKGYCF VSYLAHLVGD QDQFDKFLKA YVDEFKFQSI 450
    LAEDFLEFYL EYFPELKKKG VDSIPGFEFD RWLNTPGWPP YLPDLSPGDS 500
    LMKPAEELAE LWVTSEPDMQ AIEAVAISTW KTYQLVYFLD KILQKSPLPP 550
    GNVKKLGETY PKISNAQNAE LRLRWGQIIL KNDYQEEFQK VKDFLQSQGK 600
    QKYTLPLYHA MMGGSEMART LAKDTFAATA SQLHSNVVNY VQQILAPKDS 650
    Length:650
    Mass (Da):72,416
    Last modified:July 27, 2011 - v2
    Checksum:i95138D47E57E3C61
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 111A → G in AAH19200. (PubMed:15489334)Curated
    Sequence conflicti127 – 1271R → P in AAH19200. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK159195 mRNA. Translation: BAE34890.1.
    AK159445 mRNA. Translation: BAE35089.1.
    AK159625 mRNA. Translation: BAE35239.1.
    CH466520 Genomic DNA. Translation: EDL39584.1.
    BC019200 mRNA. Translation: AAH19200.1.
    CCDSiCCDS15317.1.
    RefSeqiNP_001153096.1. NM_001159624.1.
    NP_663392.2. NM_145417.3.
    UniGeneiMm.291048.

    Genome annotation databases

    EnsembliENSMUST00000077340; ENSMUSP00000076564; ENSMUSG00000041926.
    GeneIDi215615.
    KEGGimmu:215615.
    UCSCiuc007csy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK159195 mRNA. Translation: BAE34890.1 .
    AK159445 mRNA. Translation: BAE35089.1 .
    AK159625 mRNA. Translation: BAE35239.1 .
    CH466520 Genomic DNA. Translation: EDL39584.1 .
    BC019200 mRNA. Translation: AAH19200.1 .
    CCDSi CCDS15317.1.
    RefSeqi NP_001153096.1. NM_001159624.1.
    NP_663392.2. NM_145417.3.
    UniGenei Mm.291048.

    3D structure databases

    ProteinModelPortali Q8VCT3.
    SMRi Q8VCT3. Positions 61-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 229640. 1 interaction.
    IntActi Q8VCT3. 2 interactions.
    MINTi MINT-4087642.
    STRINGi 10090.ENSMUSP00000076564.

    Chemistry

    BindingDBi Q8VCT3.
    ChEMBLi CHEMBL2836.

    Protein family/group databases

    MEROPSi M01.014.

    PTM databases

    PhosphoSitei Q8VCT3.

    Proteomic databases

    MaxQBi Q8VCT3.
    PaxDbi Q8VCT3.
    PRIDEi Q8VCT3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000077340 ; ENSMUSP00000076564 ; ENSMUSG00000041926 .
    GeneIDi 215615.
    KEGGi mmu:215615.
    UCSCi uc007csy.2. mouse.

    Organism-specific databases

    CTDi 6051.
    MGIi MGI:2384902. Rnpep.

    Phylogenomic databases

    eggNOGi COG0308.
    GeneTreei ENSGT00530000063003.
    HOGENOMi HOG000293296.
    HOVERGENi HBG001274.
    InParanoidi Q3TX27.
    KOi K01260.
    OMAi PPGNVKK.
    OrthoDBi EOG7SJD42.
    TreeFami TF300758.

    Miscellaneous databases

    NextBioi 374775.
    PROi Q8VCT3.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VCT3.
    Bgeei Q8VCT3.
    CleanExi MM_RNPEP.
    Genevestigatori Q8VCT3.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR015571. Pept_M1_aminopeptidase-B.
    IPR001930. Peptidase_M1.
    IPR015211. Peptidase_M1_C.
    IPR014782. Peptidase_M1_N.
    [Graphical view ]
    PANTHERi PTHR11533. PTHR11533. 1 hit.
    PTHR11533:SF153. PTHR11533:SF153. 1 hit.
    Pfami PF09127. Leuk-A4-hydro_C. 1 hit.
    PF01433. Peptidase_M1. 1 hit.
    [Graphical view ]
    PRINTSi PR00756. ALADIPTASE.
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiAMPB_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCT3
    Secondary accession number(s): Q3TX27
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 11, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3