Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8VCT3

- AMPB_MOUSE

UniProt

Q8VCT3 - AMPB_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Aminopeptidase B

Gene

Rnpep

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Exopeptidase which selectively removes arginine and/or lysine residues from the N-terminus of several peptide substrates including Arg(0)-Leu-enkephalin, Arg(0)-Met-enkephalin and Arg(-1)-Lys(0)-somatostatin-14. Can hydrolyze leukotriene A4 (LTA-4) into leukotriene B4 (LTB-4) (By similarity).By similarity

Catalytic activityi

Release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys.

Cofactori

Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi325 – 3251Zinc; catalyticPROSITE-ProRule annotation
Active sitei326 – 3261Proton acceptorPROSITE-ProRule annotation
Metal bindingi329 – 3291Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi348 – 3481Zinc; catalyticPROSITE-ProRule annotation
Sitei414 – 4141Transition state stabilizerBy similarity

GO - Molecular functioni

  1. aminopeptidase activity Source: UniProtKB
  2. metallopeptidase activity Source: UniProtKB-KW
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. leukotriene biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiM01.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Aminopeptidase B (EC:3.4.11.6)
Short name:
AP-B
Alternative name(s):
Arginine aminopeptidase
Arginyl aminopeptidase
Cytosol aminopeptidase IV
Gene namesi
Name:Rnpep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:2384902. Rnpep.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
  2. extracellular space Source: InterPro
  3. extracellular vesicular exosome Source: Ensembl
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 650649Aminopeptidase BPRO_0000095089Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei446 – 4461N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiQ8VCT3.
PaxDbiQ8VCT3.
PRIDEiQ8VCT3.

PTM databases

PhosphoSiteiQ8VCT3.

Expressioni

Gene expression databases

BgeeiQ8VCT3.
CleanExiMM_RNPEP.
ExpressionAtlasiQ8VCT3. baseline and differential.
GenevestigatoriQ8VCT3.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi229640. 1 interaction.
IntActiQ8VCT3. 2 interactions.
MINTiMINT-4087642.
STRINGi10090.ENSMUSP00000076564.

Structurei

3D structure databases

ProteinModelPortaliQ8VCT3.
SMRiQ8VCT3. Positions 61-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3025Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the peptidase M1 family.Curated

Phylogenomic databases

eggNOGiCOG0308.
GeneTreeiENSGT00530000063003.
HOGENOMiHOG000293296.
HOVERGENiHBG001274.
InParanoidiQ8VCT3.
KOiK01260.
OMAiPPGNVKK.
OrthoDBiEOG7SJD42.
TreeFamiTF300758.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR015571. Pept_M1_aminopeptidase-B.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
[Graphical view]
PANTHERiPTHR11533. PTHR11533. 1 hit.
PTHR11533:SF153. PTHR11533:SF153. 1 hit.
PfamiPF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view]
PRINTSiPR00756. ALADIPTASE.
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VCT3 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MESGGPGNYS AAARRPLHSA QAVDVASASS FRAFEILHLH LDLRAEFGPP
60 70 80 90 100
GPGPGSRGLS GTATLELRCL LPEGASELRL DSHSCLEVTA ATLRRGQPGD
110 120 130 140 150
QQAPAEPVPF HTQPFSHYGQ ALCVAFRQPC GAADRFELEL TYRVGEGPGV
160 170 180 190 200
CWLAPEQTAG KKKPFVYTQG QAVLNRAFFP CFDTPAVKCT YSALIEVPDG
210 220 230 240 250
FTAVMSADTW EKRGPNKFFF QMSHPIPSYL IALAIGDLAS AEVGPRSRVW
260 270 280 290 300
AEPCLIEAAK EEYSGVIEEF LATGEKLFGP YVWGRYDLLF MPPSFPFGGM
310 320 330 340 350
ENPCLTFVTP CLLAGDRSLA DVIIHEISHS WFGNLVTNAN WGEFWLNEGF
360 370 380 390 400
TMYAQRRIST ILFGAAYTCL EAATGRALLR QHMNVSGEEN PLNKLRVKIE
410 420 430 440 450
PGVDPDDTYN ETPYEKGYCF VSYLAHLVGD QDQFDKFLKA YVDEFKFQSI
460 470 480 490 500
LAEDFLEFYL EYFPELKKKG VDSIPGFEFD RWLNTPGWPP YLPDLSPGDS
510 520 530 540 550
LMKPAEELAE LWVTSEPDMQ AIEAVAISTW KTYQLVYFLD KILQKSPLPP
560 570 580 590 600
GNVKKLGETY PKISNAQNAE LRLRWGQIIL KNDYQEEFQK VKDFLQSQGK
610 620 630 640 650
QKYTLPLYHA MMGGSEMART LAKDTFAATA SQLHSNVVNY VQQILAPKDS
Length:650
Mass (Da):72,416
Last modified:July 27, 2011 - v2
Checksum:i95138D47E57E3C61
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111A → G in AAH19200. (PubMed:15489334)Curated
Sequence conflicti127 – 1271R → P in AAH19200. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK159195 mRNA. Translation: BAE34890.1.
AK159445 mRNA. Translation: BAE35089.1.
AK159625 mRNA. Translation: BAE35239.1.
CH466520 Genomic DNA. Translation: EDL39584.1.
BC019200 mRNA. Translation: AAH19200.1.
CCDSiCCDS15317.1.
RefSeqiNP_001153096.1. NM_001159624.1.
NP_663392.2. NM_145417.3.
UniGeneiMm.291048.

Genome annotation databases

EnsembliENSMUST00000077340; ENSMUSP00000076564; ENSMUSG00000041926.
GeneIDi215615.
KEGGimmu:215615.
UCSCiuc007csy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK159195 mRNA. Translation: BAE34890.1 .
AK159445 mRNA. Translation: BAE35089.1 .
AK159625 mRNA. Translation: BAE35239.1 .
CH466520 Genomic DNA. Translation: EDL39584.1 .
BC019200 mRNA. Translation: AAH19200.1 .
CCDSi CCDS15317.1.
RefSeqi NP_001153096.1. NM_001159624.1.
NP_663392.2. NM_145417.3.
UniGenei Mm.291048.

3D structure databases

ProteinModelPortali Q8VCT3.
SMRi Q8VCT3. Positions 61-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 229640. 1 interaction.
IntActi Q8VCT3. 2 interactions.
MINTi MINT-4087642.
STRINGi 10090.ENSMUSP00000076564.

Chemistry

BindingDBi Q8VCT3.
ChEMBLi CHEMBL2836.

Protein family/group databases

MEROPSi M01.014.

PTM databases

PhosphoSitei Q8VCT3.

Proteomic databases

MaxQBi Q8VCT3.
PaxDbi Q8VCT3.
PRIDEi Q8VCT3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000077340 ; ENSMUSP00000076564 ; ENSMUSG00000041926 .
GeneIDi 215615.
KEGGi mmu:215615.
UCSCi uc007csy.2. mouse.

Organism-specific databases

CTDi 6051.
MGIi MGI:2384902. Rnpep.

Phylogenomic databases

eggNOGi COG0308.
GeneTreei ENSGT00530000063003.
HOGENOMi HOG000293296.
HOVERGENi HBG001274.
InParanoidi Q8VCT3.
KOi K01260.
OMAi PPGNVKK.
OrthoDBi EOG7SJD42.
TreeFami TF300758.

Miscellaneous databases

NextBioi 374775.
PROi Q8VCT3.
SOURCEi Search...

Gene expression databases

Bgeei Q8VCT3.
CleanExi MM_RNPEP.
ExpressionAtlasi Q8VCT3. baseline and differential.
Genevestigatori Q8VCT3.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR015571. Pept_M1_aminopeptidase-B.
IPR001930. Peptidase_M1.
IPR015211. Peptidase_M1_C.
IPR014782. Peptidase_M1_N.
[Graphical view ]
PANTHERi PTHR11533. PTHR11533. 1 hit.
PTHR11533:SF153. PTHR11533:SF153. 1 hit.
Pfami PF09127. Leuk-A4-hydro_C. 1 hit.
PF01433. Peptidase_M1. 1 hit.
[Graphical view ]
PRINTSi PR00756. ALADIPTASE.
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiAMPB_MOUSE
AccessioniPrimary (citable) accession number: Q8VCT3
Secondary accession number(s): Q3TX27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3