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Protein

N-acetylmuramoyl-L-alanine amidase

Gene

Pglyrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi391 – 3911ZincBy similarity
Metal bindingi427 – 4271ZincBy similarity
Metal bindingi502 – 5021ZincBy similarity
Metal bindingi510 – 5101ZincBy similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC
  2. peptidoglycan binding Source: UniProtKB
  3. peptidoglycan receptor activity Source: UniProtKB
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. defense response to Gram-positive bacterium Source: UniProtKB
  2. detection of bacterium Source: UniProtKB
  3. growth of symbiont in host Source: MGI
  4. negative regulation of interferon-gamma production Source: MGI
  5. negative regulation of natural killer cell differentiation involved in immune response Source: MGI
  6. pattern recognition receptor signaling pathway Source: MGI
  7. peptidoglycan catabolic process Source: InterPro
  8. regulation of inflammatory response Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Peptidoglycan recognition protein 2
Peptidoglycan recognition protein long
Short name:
PGRP-L
TagL
Gene namesi
Name:Pglyrp2
Synonyms:Pglyrpl, Pgrpl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1928099. Pglyrp2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 22221 PublicationAdd
BLAST
Chaini23 – 530508N-acetylmuramoyl-L-alanine amidasePRO_0000023921Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
Modified residuei219 – 2191PhosphoserineBy similarity
Glycosylationi335 – 3351N-linked (GlcNAc...)1 Publication
Disulfide bondi399 ↔ 405By similarity
Glycosylationi465 – 4651N-linked (GlcNAc...)By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ8VCS0.
PaxDbiQ8VCS0.
PRIDEiQ8VCS0.

Expressioni

Tissue specificityi

Strongly expressed in liver and fetal liver.

Gene expression databases

BgeeiQ8VCS0.
CleanExiMM_PGLYRP2.
ExpressionAtlasiQ8VCS0. baseline and differential.
GenevestigatoriQ8VCS0.

Structurei

3D structure databases

ProteinModelPortaliQ8VCS0.
SMRiQ8VCS0. Positions 359-525.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG248951.
GeneTreeiENSGT00390000016833.
HOGENOMiHOG000276878.
HOVERGENiHBG053578.
InParanoidiQ8VCS0.
KOiK01446.
OMAiTEAFLGC.
OrthoDBiEOG757CZ5.
PhylomeDBiQ8VCS0.
TreeFamiTF323898.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VCS0-1) [UniParc]FASTAAdd to basket

Also known as: TagL-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKAWGALWIV LGLLLWPEPG AASSLPLLMD SIIQALAELE QKVPVTEASI
60 70 80 90 100
TASAWILSAK NSSTHNSLHQ RLLLKAPSHN TTEPDPHSLS PELQALISEV
110 120 130 140 150
AQHDVQNGRE YGVVLAPDGS TVAVKPLLFG LEAGLQAHSV ANLPSDCLAI
160 170 180 190 200
PCDTGDTLAN IRATWPGLMD AFPNASSPDV GATLPNDKAK TPTTVDRLLA
210 220 230 240 250
ITLAGDLGLT FLHRSQTWSP PGLGTEGCWD QLTAPRVFTL LDPQASRLTM
260 270 280 290 300
AFLNGALDGA LLGNHLSQIP RPHPPLSHLL REYYGAGVNG DPVFRSNFRR
310 320 330 340 350
QNGAALTSAP TLAQQVWEAL VLLQKLEPEH LQLQNISQEQ LAQVATLATK
360 370 380 390 400
EFTEAFLGCP AIHPRCRWGA APYRGHPTPL RLPLGFLYVH HTYVPAPPCT
410 420 430 440 450
TFQSCAADMR SMQRFHQDVR KWDDIGYSFV VGSDGYLYQG RGWHWVGAHT
460 470 480 490 500
RGYNSRGFGV AFVGNYTGSL PNEAALNTVR DALPSCAIRA GLLRPDYKLL
510 520 530
GHRQLVLTHC PGNALFNLLR TWPHFTEVEN
Length:530
Mass (Da):57,707
Last modified:March 1, 2002 - v1
Checksum:iD3BF52597CE5D1F9
GO
Isoform 2 (identifier: Q8VCS0-2) [UniParc]FASTAAdd to basket

Also known as: TagL-beta

The sequence of this isoform differs from the canonical sequence as follows:
     338-366: Missing.

Show »
Length:501
Mass (Da):54,551
Checksum:i83886A94BEA80347
GO
Isoform 3 (identifier: Q8VCS0-3) [UniParc]FASTAAdd to basket

Also known as: TagL-epsilon

The sequence of this isoform differs from the canonical sequence as follows:
     428-450: SFVVGSDGYLYQGRGWHWVGAHT → RLKTKNSFERPLKIQEVLSLMIL
     451-530: Missing.

Show »
Length:450
Mass (Da):49,063
Checksum:iA5C5E0D48E4287B1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti486 – 4861Missing in AAF22233 (PubMed:12559914).Curated
Sequence conflicti486 – 4861Missing in AAF22234 (PubMed:12559914).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei338 – 36629Missing in isoform 2. 1 PublicationVSP_009081Add
BLAST
Alternative sequencei428 – 45023SFVVG…VGAHT → RLKTKNSFERPLKIQEVLSL MIL in isoform 3. 1 PublicationVSP_009079Add
BLAST
Alternative sequencei451 – 53080Missing in isoform 3. 1 PublicationVSP_009080Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY282722 mRNA. Translation: AAP22283.1.
AF392055 mRNA. Translation: AAM73674.1.
AF149837 mRNA. Translation: AAF22233.1.
AF149838 mRNA. Translation: AAF22234.1.
BC019396 mRNA. Translation: AAH19396.1.
CCDSiCCDS37557.1. [Q8VCS0-1]
RefSeqiNP_001258405.1. NM_001271476.1. [Q8VCS0-1]
NP_001258407.1. NM_001271478.1. [Q8VCS0-3]
NP_001258408.1. NM_001271479.1.
NP_067294.2. NM_021319.5. [Q8VCS0-1]
XP_006524777.1. XM_006524714.1. [Q8VCS0-3]
UniGeneiMm.86752.

Genome annotation databases

EnsembliENSMUST00000114455; ENSMUSP00000110099; ENSMUSG00000079563. [Q8VCS0-1]
ENSMUST00000170392; ENSMUSP00000129964; ENSMUSG00000079563. [Q8VCS0-1]
GeneIDi57757.
KEGGimmu:57757.
UCSCiuc008bxa.2. mouse. [Q8VCS0-1]
uc008bxc.2. mouse. [Q8VCS0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY282722 mRNA. Translation: AAP22283.1.
AF392055 mRNA. Translation: AAM73674.1.
AF149837 mRNA. Translation: AAF22233.1.
AF149838 mRNA. Translation: AAF22234.1.
BC019396 mRNA. Translation: AAH19396.1.
CCDSiCCDS37557.1. [Q8VCS0-1]
RefSeqiNP_001258405.1. NM_001271476.1. [Q8VCS0-1]
NP_001258407.1. NM_001271478.1. [Q8VCS0-3]
NP_001258408.1. NM_001271479.1.
NP_067294.2. NM_021319.5. [Q8VCS0-1]
XP_006524777.1. XM_006524714.1. [Q8VCS0-3]
UniGeneiMm.86752.

3D structure databases

ProteinModelPortaliQ8VCS0.
SMRiQ8VCS0. Positions 359-525.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

MaxQBiQ8VCS0.
PaxDbiQ8VCS0.
PRIDEiQ8VCS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114455; ENSMUSP00000110099; ENSMUSG00000079563. [Q8VCS0-1]
ENSMUST00000170392; ENSMUSP00000129964; ENSMUSG00000079563. [Q8VCS0-1]
GeneIDi57757.
KEGGimmu:57757.
UCSCiuc008bxa.2. mouse. [Q8VCS0-1]
uc008bxc.2. mouse. [Q8VCS0-3]

Organism-specific databases

CTDi114770.
MGIiMGI:1928099. Pglyrp2.

Phylogenomic databases

eggNOGiNOG248951.
GeneTreeiENSGT00390000016833.
HOGENOMiHOG000276878.
HOVERGENiHBG053578.
InParanoidiQ8VCS0.
KOiK01446.
OMAiTEAFLGC.
OrthoDBiEOG757CZ5.
PhylomeDBiQ8VCS0.
TreeFamiTF323898.

Miscellaneous databases

NextBioi313928.
PROiQ8VCS0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCS0.
CleanExiMM_PGLYRP2.
ExpressionAtlasiQ8VCS0. baseline and differential.
GenevestigatoriQ8VCS0.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A mammalian peptidoglycan recognition protein with N-acetylmuramoyl-L-alanine amidase activity."
    Gelius E., Persson C., Karlsson J., Steiner H.
    Biochem. Biophys. Res. Commun. 306:988-994(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 23-37.
    Strain: C57BL/6J.
  2. "The differentially spliced mouse tagL gene, homolog of tag7/PGRP gene family in mammals and Drosophila, can recognize Gram-positive and Gram-negative bacterial cell wall independently of T phage lysozyme homology domain."
    Kibardin A.V., Mirkina I.I., Baranova E.V., Zakeyeva I.R., Georgiev G.P., Kiselev S.L.
    J. Mol. Biol. 326:467-474(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Liver.
  4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-335.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiPGRP2_MOUSE
AccessioniPrimary (citable) accession number: Q8VCS0
Secondary accession number(s): Q8K4I8, Q9QXZ1, Q9QXZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2002
Last modified: March 4, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.