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Q8VCS0

- PGRP2_MOUSE

UniProt

Q8VCS0 - PGRP2_MOUSE

Protein

N-acetylmuramoyl-L-alanine amidase

Gene

Pglyrp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi391 – 3911ZincBy similarity
    Metal bindingi427 – 4271ZincBy similarity
    Metal bindingi502 – 5021ZincBy similarity
    Metal bindingi510 – 5101ZincBy similarity

    GO - Molecular functioni

    1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC
    2. peptidoglycan binding Source: UniProtKB
    3. peptidoglycan receptor activity Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. defense response to Gram-positive bacterium Source: UniProtKB
    2. detection of bacterium Source: UniProtKB
    3. growth of symbiont in host Source: MGI
    4. negative regulation of interferon-gamma production Source: MGI
    5. negative regulation of natural killer cell differentiation involved in immune response Source: MGI
    6. pattern recognition receptor signaling pathway Source: GOC
    7. peptidoglycan catabolic process Source: InterPro
    8. regulation of inflammatory response Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Immunity

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
    Alternative name(s):
    Peptidoglycan recognition protein 2
    Peptidoglycan recognition protein long
    Short name:
    PGRP-L
    TagL
    Gene namesi
    Name:Pglyrp2
    Synonyms:Pglyrpl, Pgrpl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1928099. Pglyrp2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 22221 PublicationAdd
    BLAST
    Chaini23 – 530508N-acetylmuramoyl-L-alanine amidasePRO_0000023921Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi174 – 1741N-linked (GlcNAc...)Sequence Analysis
    Modified residuei219 – 2191PhosphoserineBy similarity
    Glycosylationi335 – 3351N-linked (GlcNAc...)1 Publication
    Disulfide bondi399 ↔ 405By similarity
    Glycosylationi465 – 4651N-linked (GlcNAc...)By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ8VCS0.
    PRIDEiQ8VCS0.

    Expressioni

    Tissue specificityi

    Strongly expressed in liver and fetal liver.

    Gene expression databases

    ArrayExpressiQ8VCS0.
    BgeeiQ8VCS0.
    CleanExiMM_PGLYRP2.
    GenevestigatoriQ8VCS0.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCS0.
    SMRiQ8VCS0. Positions 359-525.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG248951.
    GeneTreeiENSGT00390000016833.
    HOGENOMiHOG000276878.
    HOVERGENiHBG053578.
    InParanoidiQ8VCS0.
    KOiK01446.
    OMAiMAFLNGA.
    OrthoDBiEOG757CZ5.
    PhylomeDBiQ8VCS0.
    TreeFamiTF323898.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    IPR015510. PGRP.
    IPR006619. PGRP_domain_met/bac.
    [Graphical view]
    PANTHERiPTHR11022. PTHR11022. 1 hit.
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    SM00701. PGRP. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VCS0-1) [UniParc]FASTAAdd to Basket

    Also known as: TagL-alpha

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKAWGALWIV LGLLLWPEPG AASSLPLLMD SIIQALAELE QKVPVTEASI    50
    TASAWILSAK NSSTHNSLHQ RLLLKAPSHN TTEPDPHSLS PELQALISEV 100
    AQHDVQNGRE YGVVLAPDGS TVAVKPLLFG LEAGLQAHSV ANLPSDCLAI 150
    PCDTGDTLAN IRATWPGLMD AFPNASSPDV GATLPNDKAK TPTTVDRLLA 200
    ITLAGDLGLT FLHRSQTWSP PGLGTEGCWD QLTAPRVFTL LDPQASRLTM 250
    AFLNGALDGA LLGNHLSQIP RPHPPLSHLL REYYGAGVNG DPVFRSNFRR 300
    QNGAALTSAP TLAQQVWEAL VLLQKLEPEH LQLQNISQEQ LAQVATLATK 350
    EFTEAFLGCP AIHPRCRWGA APYRGHPTPL RLPLGFLYVH HTYVPAPPCT 400
    TFQSCAADMR SMQRFHQDVR KWDDIGYSFV VGSDGYLYQG RGWHWVGAHT 450
    RGYNSRGFGV AFVGNYTGSL PNEAALNTVR DALPSCAIRA GLLRPDYKLL 500
    GHRQLVLTHC PGNALFNLLR TWPHFTEVEN 530
    Length:530
    Mass (Da):57,707
    Last modified:March 1, 2002 - v1
    Checksum:iD3BF52597CE5D1F9
    GO
    Isoform 2 (identifier: Q8VCS0-2) [UniParc]FASTAAdd to Basket

    Also known as: TagL-beta

    The sequence of this isoform differs from the canonical sequence as follows:
         338-366: Missing.

    Show »
    Length:501
    Mass (Da):54,551
    Checksum:i83886A94BEA80347
    GO
    Isoform 3 (identifier: Q8VCS0-3) [UniParc]FASTAAdd to Basket

    Also known as: TagL-epsilon

    The sequence of this isoform differs from the canonical sequence as follows:
         428-450: SFVVGSDGYLYQGRGWHWVGAHT → RLKTKNSFERPLKIQEVLSLMIL
         451-530: Missing.

    Show »
    Length:450
    Mass (Da):49,063
    Checksum:iA5C5E0D48E4287B1
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti486 – 4861Missing in AAF22233. (PubMed:12559914)Curated
    Sequence conflicti486 – 4861Missing in AAF22234. (PubMed:12559914)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei338 – 36629Missing in isoform 2. 1 PublicationVSP_009081Add
    BLAST
    Alternative sequencei428 – 45023SFVVG…VGAHT → RLKTKNSFERPLKIQEVLSL MIL in isoform 3. 1 PublicationVSP_009079Add
    BLAST
    Alternative sequencei451 – 53080Missing in isoform 3. 1 PublicationVSP_009080Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY282722 mRNA. Translation: AAP22283.1.
    AF392055 mRNA. Translation: AAM73674.1.
    AF149837 mRNA. Translation: AAF22233.1.
    AF149838 mRNA. Translation: AAF22234.1.
    BC019396 mRNA. Translation: AAH19396.1.
    CCDSiCCDS37557.1. [Q8VCS0-1]
    RefSeqiNP_001258405.1. NM_001271476.1. [Q8VCS0-1]
    NP_001258407.1. NM_001271478.1. [Q8VCS0-3]
    NP_001258408.1. NM_001271479.1.
    NP_067294.2. NM_021319.5. [Q8VCS0-1]
    XP_006524777.1. XM_006524714.1. [Q8VCS0-3]
    UniGeneiMm.86752.

    Genome annotation databases

    EnsembliENSMUST00000114455; ENSMUSP00000110099; ENSMUSG00000079563. [Q8VCS0-1]
    ENSMUST00000170392; ENSMUSP00000129964; ENSMUSG00000079563. [Q8VCS0-1]
    GeneIDi57757.
    KEGGimmu:57757.
    UCSCiuc008bxa.2. mouse. [Q8VCS0-1]
    uc008bxc.2. mouse. [Q8VCS0-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY282722 mRNA. Translation: AAP22283.1 .
    AF392055 mRNA. Translation: AAM73674.1 .
    AF149837 mRNA. Translation: AAF22233.1 .
    AF149838 mRNA. Translation: AAF22234.1 .
    BC019396 mRNA. Translation: AAH19396.1 .
    CCDSi CCDS37557.1. [Q8VCS0-1 ]
    RefSeqi NP_001258405.1. NM_001271476.1. [Q8VCS0-1 ]
    NP_001258407.1. NM_001271478.1. [Q8VCS0-3 ]
    NP_001258408.1. NM_001271479.1.
    NP_067294.2. NM_021319.5. [Q8VCS0-1 ]
    XP_006524777.1. XM_006524714.1. [Q8VCS0-3 ]
    UniGenei Mm.86752.

    3D structure databases

    ProteinModelPortali Q8VCS0.
    SMRi Q8VCS0. Positions 359-525.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q8VCS0.
    PRIDEi Q8VCS0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000114455 ; ENSMUSP00000110099 ; ENSMUSG00000079563 . [Q8VCS0-1 ]
    ENSMUST00000170392 ; ENSMUSP00000129964 ; ENSMUSG00000079563 . [Q8VCS0-1 ]
    GeneIDi 57757.
    KEGGi mmu:57757.
    UCSCi uc008bxa.2. mouse. [Q8VCS0-1 ]
    uc008bxc.2. mouse. [Q8VCS0-3 ]

    Organism-specific databases

    CTDi 114770.
    MGIi MGI:1928099. Pglyrp2.

    Phylogenomic databases

    eggNOGi NOG248951.
    GeneTreei ENSGT00390000016833.
    HOGENOMi HOG000276878.
    HOVERGENi HBG053578.
    InParanoidi Q8VCS0.
    KOi K01446.
    OMAi MAFLNGA.
    OrthoDBi EOG757CZ5.
    PhylomeDBi Q8VCS0.
    TreeFami TF323898.

    Miscellaneous databases

    NextBioi 313928.
    PROi Q8VCS0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VCS0.
    Bgeei Q8VCS0.
    CleanExi MM_PGLYRP2.
    Genevestigatori Q8VCS0.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    IPR015510. PGRP.
    IPR006619. PGRP_domain_met/bac.
    [Graphical view ]
    PANTHERi PTHR11022. PTHR11022. 1 hit.
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    SM00701. PGRP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A mammalian peptidoglycan recognition protein with N-acetylmuramoyl-L-alanine amidase activity."
      Gelius E., Persson C., Karlsson J., Steiner H.
      Biochem. Biophys. Res. Commun. 306:988-994(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 23-37.
      Strain: C57BL/6J.
    2. "The differentially spliced mouse tagL gene, homolog of tag7/PGRP gene family in mammals and Drosophila, can recognize Gram-positive and Gram-negative bacterial cell wall independently of T phage lysozyme homology domain."
      Kibardin A.V., Mirkina I.I., Baranova E.V., Zakeyeva I.R., Georgiev G.P., Kiselev S.L.
      J. Mol. Biol. 326:467-474(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Liver.
    4. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
      Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
      J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-335.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiPGRP2_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCS0
    Secondary accession number(s): Q8K4I8, Q9QXZ1, Q9QXZ2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2003
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3