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Protein

N-acetylmuramoyl-L-alanine amidase

Gene

Pglyrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zn2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi391ZincBy similarity1
Metal bindingi427ZincBy similarity1
Metal bindingi502ZincBy similarity1
Metal bindingi510ZincBy similarity1

GO - Molecular functioni

GO - Biological processi

  • defense response to Gram-positive bacterium Source: UniProtKB
  • detection of bacterium Source: UniProtKB
  • growth of symbiont in host Source: MGI
  • negative regulation of interferon-gamma production Source: MGI
  • negative regulation of natural killer cell differentiation involved in immune response Source: MGI
  • peptidoglycan catabolic process Source: InterPro
  • regulation of inflammatory response Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Immunity

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylmuramoyl-L-alanine amidase (EC:3.5.1.28)
Alternative name(s):
Peptidoglycan recognition protein 2
Peptidoglycan recognition protein long
Short name:
PGRP-L
TagL
Gene namesi
Name:Pglyrp2
Synonyms:Pglyrpl, Pgrpl
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1928099. Pglyrp2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000002392123 – 530N-acetylmuramoyl-L-alanine amidaseAdd BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Glycosylationi174N-linked (GlcNAc...)Sequence analysis1
Modified residuei219PhosphoserineBy similarity1
Glycosylationi335N-linked (GlcNAc...)1 Publication1
Disulfide bondi399 ↔ 405By similarity
Glycosylationi465N-linked (GlcNAc...)By similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiQ8VCS0.
PaxDbiQ8VCS0.
PeptideAtlasiQ8VCS0.
PRIDEiQ8VCS0.

PTM databases

PhosphoSitePlusiQ8VCS0.

Expressioni

Tissue specificityi

Strongly expressed in liver and fetal liver.

Gene expression databases

BgeeiENSMUSG00000079563.
CleanExiMM_PGLYRP2.
GenevisibleiQ8VCS0. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110099.

Structurei

3D structure databases

ProteinModelPortaliQ8VCS0.
SMRiQ8VCS0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IIH1. Eukaryota.
ENOG4111PAY. LUCA.
GeneTreeiENSGT00390000016833.
HOGENOMiHOG000276878.
HOVERGENiHBG053578.
InParanoidiQ8VCS0.
KOiK01446.
OMAiTEAFLGC.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ8VCS0.
TreeFamiTF323898.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VCS0-1) [UniParc]FASTAAdd to basket
Also known as: TagL-alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKAWGALWIV LGLLLWPEPG AASSLPLLMD SIIQALAELE QKVPVTEASI
60 70 80 90 100
TASAWILSAK NSSTHNSLHQ RLLLKAPSHN TTEPDPHSLS PELQALISEV
110 120 130 140 150
AQHDVQNGRE YGVVLAPDGS TVAVKPLLFG LEAGLQAHSV ANLPSDCLAI
160 170 180 190 200
PCDTGDTLAN IRATWPGLMD AFPNASSPDV GATLPNDKAK TPTTVDRLLA
210 220 230 240 250
ITLAGDLGLT FLHRSQTWSP PGLGTEGCWD QLTAPRVFTL LDPQASRLTM
260 270 280 290 300
AFLNGALDGA LLGNHLSQIP RPHPPLSHLL REYYGAGVNG DPVFRSNFRR
310 320 330 340 350
QNGAALTSAP TLAQQVWEAL VLLQKLEPEH LQLQNISQEQ LAQVATLATK
360 370 380 390 400
EFTEAFLGCP AIHPRCRWGA APYRGHPTPL RLPLGFLYVH HTYVPAPPCT
410 420 430 440 450
TFQSCAADMR SMQRFHQDVR KWDDIGYSFV VGSDGYLYQG RGWHWVGAHT
460 470 480 490 500
RGYNSRGFGV AFVGNYTGSL PNEAALNTVR DALPSCAIRA GLLRPDYKLL
510 520 530
GHRQLVLTHC PGNALFNLLR TWPHFTEVEN
Length:530
Mass (Da):57,707
Last modified:March 1, 2002 - v1
Checksum:iD3BF52597CE5D1F9
GO
Isoform 2 (identifier: Q8VCS0-2) [UniParc]FASTAAdd to basket
Also known as: TagL-beta

The sequence of this isoform differs from the canonical sequence as follows:
     338-366: Missing.

Show »
Length:501
Mass (Da):54,551
Checksum:i83886A94BEA80347
GO
Isoform 3 (identifier: Q8VCS0-3) [UniParc]FASTAAdd to basket
Also known as: TagL-epsilon

The sequence of this isoform differs from the canonical sequence as follows:
     428-450: SFVVGSDGYLYQGRGWHWVGAHT → RLKTKNSFERPLKIQEVLSLMIL
     451-530: Missing.

Show »
Length:450
Mass (Da):49,063
Checksum:iA5C5E0D48E4287B1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti486Missing in AAF22233 (PubMed:12559914).Curated1
Sequence conflicti486Missing in AAF22234 (PubMed:12559914).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_009081338 – 366Missing in isoform 2. 1 PublicationAdd BLAST29
Alternative sequenceiVSP_009079428 – 450SFVVG…VGAHT → RLKTKNSFERPLKIQEVLSL MIL in isoform 3. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_009080451 – 530Missing in isoform 3. 1 PublicationAdd BLAST80

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY282722 mRNA. Translation: AAP22283.1.
AF392055 mRNA. Translation: AAM73674.1.
AF149837 mRNA. Translation: AAF22233.1.
AF149838 mRNA. Translation: AAF22234.1.
BC019396 mRNA. Translation: AAH19396.1.
CCDSiCCDS37557.1. [Q8VCS0-1]
RefSeqiNP_001258405.1. NM_001271476.1. [Q8VCS0-1]
NP_001258407.1. NM_001271478.1. [Q8VCS0-3]
NP_001258408.1. NM_001271479.1.
NP_067294.2. NM_021319.5. [Q8VCS0-1]
XP_006524777.1. XM_006524714.3. [Q8VCS0-3]
UniGeneiMm.86752.

Genome annotation databases

EnsembliENSMUST00000114455; ENSMUSP00000110099; ENSMUSG00000079563. [Q8VCS0-1]
ENSMUST00000170392; ENSMUSP00000129964; ENSMUSG00000079563. [Q8VCS0-1]
GeneIDi57757.
KEGGimmu:57757.
UCSCiuc008bxa.3. mouse. [Q8VCS0-1]
uc008bxc.3. mouse. [Q8VCS0-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY282722 mRNA. Translation: AAP22283.1.
AF392055 mRNA. Translation: AAM73674.1.
AF149837 mRNA. Translation: AAF22233.1.
AF149838 mRNA. Translation: AAF22234.1.
BC019396 mRNA. Translation: AAH19396.1.
CCDSiCCDS37557.1. [Q8VCS0-1]
RefSeqiNP_001258405.1. NM_001271476.1. [Q8VCS0-1]
NP_001258407.1. NM_001271478.1. [Q8VCS0-3]
NP_001258408.1. NM_001271479.1.
NP_067294.2. NM_021319.5. [Q8VCS0-1]
XP_006524777.1. XM_006524714.3. [Q8VCS0-3]
UniGeneiMm.86752.

3D structure databases

ProteinModelPortaliQ8VCS0.
SMRiQ8VCS0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000110099.

PTM databases

PhosphoSitePlusiQ8VCS0.

Proteomic databases

EPDiQ8VCS0.
PaxDbiQ8VCS0.
PeptideAtlasiQ8VCS0.
PRIDEiQ8VCS0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000114455; ENSMUSP00000110099; ENSMUSG00000079563. [Q8VCS0-1]
ENSMUST00000170392; ENSMUSP00000129964; ENSMUSG00000079563. [Q8VCS0-1]
GeneIDi57757.
KEGGimmu:57757.
UCSCiuc008bxa.3. mouse. [Q8VCS0-1]
uc008bxc.3. mouse. [Q8VCS0-3]

Organism-specific databases

CTDi114770.
MGIiMGI:1928099. Pglyrp2.

Phylogenomic databases

eggNOGiENOG410IIH1. Eukaryota.
ENOG4111PAY. LUCA.
GeneTreeiENSGT00390000016833.
HOGENOMiHOG000276878.
HOVERGENiHBG053578.
InParanoidiQ8VCS0.
KOiK01446.
OMAiTEAFLGC.
OrthoDBiEOG091G0O6Z.
PhylomeDBiQ8VCS0.
TreeFamiTF323898.

Enzyme and pathway databases

ReactomeiR-MMU-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiQ8VCS0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079563.
CleanExiMM_PGLYRP2.
GenevisibleiQ8VCS0. MM.

Family and domain databases

CDDicd06583. PGRP. 1 hit.
Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERiPTHR11022. PTHR11022. 1 hit.
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGRP2_MOUSE
AccessioniPrimary (citable) accession number: Q8VCS0
Secondary accession number(s): Q8K4I8, Q9QXZ1, Q9QXZ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.