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Q8VCS0 (PGRP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetylmuramoyl-L-alanine amidase

EC=3.5.1.28
Alternative name(s):
Peptidoglycan recognition protein 2
Peptidoglycan recognition protein long
Short name=PGRP-L
TagL
Gene names
Name:Pglyrp2
Synonyms:Pglyrpl, Pgrpl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May play a scavenger role by digesting biologically active peptidoglycan (PGN) into biologically inactive fragments. Has no direct bacteriolytic activity.

Catalytic activity

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactor

Zinc By similarity.

Subcellular location

Secreted. Membrane.

Tissue specificity

Strongly expressed in liver and fetal liver.

Sequence similarities

Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family.

Ontologies

Keywords
   Biological processImmunity
   Cellular componentMembrane
Secreted
   Coding sequence diversityAlternative splicing
   DomainSignal
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processdefense response to Gram-positive bacterium

Inferred from sequence or structural similarity. Source: UniProtKB

detection of bacterium

Inferred from sequence or structural similarity. Source: UniProtKB

growth of symbiont in host

Inferred from mutant phenotype PubMed 20709292. Source: MGI

negative regulation of interferon-gamma production

Inferred from mutant phenotype PubMed 20709292. Source: MGI

negative regulation of natural killer cell differentiation involved in immune response

Inferred from mutant phenotype PubMed 20709292. Source: MGI

pattern recognition receptor signaling pathway

Inferred from sequence orthology PubMed 11461926. Source: GOC

peptidoglycan catabolic process

Inferred from electronic annotation. Source: InterPro

regulation of inflammatory response

Inferred from mutant phenotype PubMed 19218085PubMed 22048773. Source: MGI

   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionN-acetylmuramoyl-L-alanine amidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

peptidoglycan binding

Inferred from sequence or structural similarity. Source: UniProtKB

peptidoglycan receptor activity

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VCS0-1)

Also known as: TagL-alpha;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VCS0-2)

Also known as: TagL-beta;

The sequence of this isoform differs from the canonical sequence as follows:
     338-366: Missing.
Isoform 3 (identifier: Q8VCS0-3)

Also known as: TagL-epsilon;

The sequence of this isoform differs from the canonical sequence as follows:
     428-450: SFVVGSDGYLYQGRGWHWVGAHT → RLKTKNSFERPLKIQEVLSLMIL
     451-530: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.1
Chain23 – 530508N-acetylmuramoyl-L-alanine amidase
PRO_0000023921

Sites

Metal binding3911Zinc By similarity
Metal binding4271Zinc By similarity
Metal binding5021Zinc By similarity
Metal binding5101Zinc By similarity

Amino acid modifications

Modified residue2191Phosphoserine By similarity
Glycosylation611N-linked (GlcNAc...) Potential
Glycosylation801N-linked (GlcNAc...) Potential
Glycosylation1741N-linked (GlcNAc...) Potential
Glycosylation3351N-linked (GlcNAc...) Ref.4
Glycosylation4651N-linked (GlcNAc...) By similarity
Disulfide bond399 ↔ 405 By similarity

Natural variations

Alternative sequence338 – 36629Missing in isoform 2.
VSP_009081
Alternative sequence428 – 45023SFVVG…VGAHT → RLKTKNSFERPLKIQEVLSL MIL in isoform 3.
VSP_009079
Alternative sequence451 – 53080Missing in isoform 3.
VSP_009080

Experimental info

Sequence conflict4861Missing in AAF22233. Ref.2
Sequence conflict4861Missing in AAF22234. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (TagL-alpha) [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: D3BF52597CE5D1F9

FASTA53057,707
        10         20         30         40         50         60 
MKAWGALWIV LGLLLWPEPG AASSLPLLMD SIIQALAELE QKVPVTEASI TASAWILSAK 

        70         80         90        100        110        120 
NSSTHNSLHQ RLLLKAPSHN TTEPDPHSLS PELQALISEV AQHDVQNGRE YGVVLAPDGS 

       130        140        150        160        170        180 
TVAVKPLLFG LEAGLQAHSV ANLPSDCLAI PCDTGDTLAN IRATWPGLMD AFPNASSPDV 

       190        200        210        220        230        240 
GATLPNDKAK TPTTVDRLLA ITLAGDLGLT FLHRSQTWSP PGLGTEGCWD QLTAPRVFTL 

       250        260        270        280        290        300 
LDPQASRLTM AFLNGALDGA LLGNHLSQIP RPHPPLSHLL REYYGAGVNG DPVFRSNFRR 

       310        320        330        340        350        360 
QNGAALTSAP TLAQQVWEAL VLLQKLEPEH LQLQNISQEQ LAQVATLATK EFTEAFLGCP 

       370        380        390        400        410        420 
AIHPRCRWGA APYRGHPTPL RLPLGFLYVH HTYVPAPPCT TFQSCAADMR SMQRFHQDVR 

       430        440        450        460        470        480 
KWDDIGYSFV VGSDGYLYQG RGWHWVGAHT RGYNSRGFGV AFVGNYTGSL PNEAALNTVR 

       490        500        510        520        530 
DALPSCAIRA GLLRPDYKLL GHRQLVLTHC PGNALFNLLR TWPHFTEVEN 

« Hide

Isoform 2 (TagL-beta) [UniParc].

Checksum: 83886A94BEA80347
Show »

FASTA50154,551
Isoform 3 (TagL-epsilon) [UniParc].

Checksum: A5C5E0D48E4287B1
Show »

FASTA45049,063

References

« Hide 'large scale' references
[1]"A mammalian peptidoglycan recognition protein with N-acetylmuramoyl-L-alanine amidase activity."
Gelius E., Persson C., Karlsson J., Steiner H.
Biochem. Biophys. Res. Commun. 306:988-994(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 23-37.
Strain: C57BL/6J.
[2]"The differentially spliced mouse tagL gene, homolog of tag7/PGRP gene family in mammals and Drosophila, can recognize Gram-positive and Gram-negative bacterial cell wall independently of T phage lysozyme homology domain."
Kibardin A.V., Mirkina I.I., Baranova E.V., Zakeyeva I.R., Georgiev G.P., Kiselev S.L.
J. Mol. Biol. 326:467-474(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Liver.
[4]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-335.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AY282722 mRNA. Translation: AAP22283.1.
AF392055 mRNA. Translation: AAM73674.1.
AF149837 mRNA. Translation: AAF22233.1.
AF149838 mRNA. Translation: AAF22234.1.
BC019396 mRNA. Translation: AAH19396.1.
CCDSCCDS37557.1. [Q8VCS0-1]
RefSeqNP_001258405.1. NM_001271476.1. [Q8VCS0-1]
NP_001258407.1. NM_001271478.1. [Q8VCS0-3]
NP_001258408.1. NM_001271479.1.
NP_067294.2. NM_021319.5. [Q8VCS0-1]
XP_006524777.1. XM_006524714.1. [Q8VCS0-3]
UniGeneMm.86752.

3D structure databases

ProteinModelPortalQ8VCS0.
SMRQ8VCS0. Positions 359-525.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PaxDbQ8VCS0.
PRIDEQ8VCS0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000114455; ENSMUSP00000110099; ENSMUSG00000079563. [Q8VCS0-1]
ENSMUST00000170392; ENSMUSP00000129964; ENSMUSG00000079563. [Q8VCS0-1]
GeneID57757.
KEGGmmu:57757.
UCSCuc008bxa.2. mouse. [Q8VCS0-1]
uc008bxc.2. mouse. [Q8VCS0-3]

Organism-specific databases

CTD114770.
MGIMGI:1928099. Pglyrp2.

Phylogenomic databases

eggNOGNOG248951.
GeneTreeENSGT00390000016833.
HOGENOMHOG000276878.
HOVERGENHBG053578.
InParanoidQ8VCS0.
KOK01446.
OMAMAFLNGA.
OrthoDBEOG757CZ5.
PhylomeDBQ8VCS0.
TreeFamTF323898.

Gene expression databases

ArrayExpressQ8VCS0.
BgeeQ8VCS0.
CleanExMM_PGLYRP2.
GenevestigatorQ8VCS0.

Family and domain databases

Gene3D3.40.80.10. 1 hit.
InterProIPR002502. Amidase_domain.
IPR015510. PGRP.
IPR006619. PGRP_domain_met/bac.
[Graphical view]
PANTHERPTHR11022. PTHR11022. 1 hit.
PfamPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTSM00644. Ami_2. 1 hit.
SM00701. PGRP. 1 hit.
[Graphical view]
SUPFAMSSF55846. SSF55846. 1 hit.
ProtoNetSearch...

Other

NextBio313928.
PROQ8VCS0.
SOURCESearch...

Entry information

Entry namePGRP2_MOUSE
AccessionPrimary (citable) accession number: Q8VCS0
Secondary accession number(s): Q8K4I8, Q9QXZ1, Q9QXZ2
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot