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Q8VCR8

- MYLK2_MOUSE

UniProt

Q8VCR8 - MYLK2_MOUSE

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Protein
Myosin light chain kinase 2, skeletal/cardiac muscle
Gene
Mylk2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain By similarity.

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei331 – 3311ATP By similarity
Active sitei423 – 4231Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi308 – 3169ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. myosin light chain kinase activity Source: UniProtKB-EC
  3. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. cardiac muscle cell differentiation Source: UniProtKB
  2. cardiac muscle tissue morphogenesis Source: Ensembl
  3. neuromuscular synaptic transmission Source: MGI
  4. peptidyl-threonine phosphorylation Source: Ensembl
  5. positive regulation of gene expression Source: Ensembl
  6. protein autophosphorylation Source: Ensembl
  7. regulation of muscle filament sliding Source: Ensembl
  8. satellite cell differentiation Source: UniProtKB
  9. skeletal muscle cell differentiation Source: UniProtKB
  10. striated muscle contraction Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
Short name:
MLCK2
Gene namesi
Name:Mylk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:2139434. Mylk2.

Subcellular locationi

Cytoplasm By similarity
Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 613612Myosin light chain kinase 2, skeletal/cardiac muscle
PRO_0000086409Add
BLAST

Proteomic databases

MaxQBiQ8VCR8.
PaxDbiQ8VCR8.
PRIDEiQ8VCR8.

PTM databases

PhosphoSiteiQ8VCR8.

Expressioni

Gene expression databases

BgeeiQ8VCR8.
GenevestigatoriQ8VCR8.

Interactioni

Subunit structurei

May interact with centrin By similarity.

Protein-protein interaction databases

IntActiQ8VCR8. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliQ8VCR8.
SMRiQ8VCR8. Positions 217-608.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini302 – 557256Protein kinase
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni591 – 60313Calmodulin-binding By similarity
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi96 – 1038Poly-Gly
Compositional biasi278 – 2858Poly-Pro

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117629.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
KOiK00907.
OMAiCFQILXG.
OrthoDBiEOG73FQMV.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VCR8-1 [UniParc]FASTAAdd to Basket

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MTTENGAVEL GSQSLSTEQT PKAAAGDGPS ASEKEPSAPA TEKDLSPPNA    50
KKDPGAPDPK NNPDPPSLKK DPAKAPGPEK KGDPVPASAS SQGPSGEGDG 100
GGGPAEGSEG PPAALPLPTA TAEASIQKLD PTQAPSGNQG SGEAKAGKKA 150
AECREAGRRG SPAFLHSPSC PAIISCSEKT LAVKPLSETT DLVFTGVSVT 200
PDPQDPGPVK AGGTNALAEK KKEEAEKASG QAGQAKVQGD TPQRIGFQAV 250
PSERVEVGQA LCLTAREEDC FQILDDCPPP PAPFPHRIVE LRTGNVNSEF 300
SMNSKEALGG GKFGAVCTCT ERATGLKLAA KVIKKQTPKD KEMVLLEIEV 350
MNQLNHRNLI QLYAAIETSH EIILFMEYIE GGELFERIVD EDYHLTEVDT 400
MVFVRQICDG ILFMHKMRVL HLDLKPENIL CVNTTGHLVK IIDFGLARRY 450
NPNEKLKVNF GTPEFLSPEV VNYDQISDKT DMWSLGVITY MLLSGLSPFL 500
GDDDTETLNN VLSANWYFDE ETFEAVSDEA KDFVSNLLTK DQSARMSAEQ 550
CLAHPWLNNL AEKAKRCNRR LKSQILLKKY LMKRRWKKNF IAVSAANRFK 600
KISSSGALMA LGV 613
Length:613
Mass (Da):65,990
Last modified:July 10, 2007 - v2
Checksum:i1FAC64C8B09DAE25
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL833801 Genomic DNA. Translation: CAM23678.1.
AK079396 mRNA. No translation available.
BC019408 mRNA. Translation: AAH19408.1.
CCDSiCCDS38282.2.
RefSeqiNP_001074513.2. NM_001081044.2.
UniGeneiMm.250604.

Genome annotation databases

EnsembliENSMUST00000028970; ENSMUSP00000028970; ENSMUSG00000027470.
GeneIDi228785.
KEGGimmu:228785.
UCSCiuc008ngs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL833801 Genomic DNA. Translation: CAM23678.1 .
AK079396 mRNA. No translation available.
BC019408 mRNA. Translation: AAH19408.1 .
CCDSi CCDS38282.2.
RefSeqi NP_001074513.2. NM_001081044.2.
UniGenei Mm.250604.

3D structure databases

ProteinModelPortali Q8VCR8.
SMRi Q8VCR8. Positions 217-608.
ModBasei Search...

Protein-protein interaction databases

IntActi Q8VCR8. 2 interactions.

PTM databases

PhosphoSitei Q8VCR8.

Proteomic databases

MaxQBi Q8VCR8.
PaxDbi Q8VCR8.
PRIDEi Q8VCR8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028970 ; ENSMUSP00000028970 ; ENSMUSG00000027470 .
GeneIDi 228785.
KEGGi mmu:228785.
UCSCi uc008ngs.2. mouse.

Organism-specific databases

CTDi 85366.
MGIi MGI:2139434. Mylk2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117629.
HOGENOMi HOG000233016.
HOVERGENi HBG080416.
KOi K00907.
OMAi CFQILXG.
OrthoDBi EOG73FQMV.
TreeFami TF314166.

Miscellaneous databases

ChiTaRSi MYLK2. mouse.
NextBioi 379136.
PROi Q8VCR8.
SOURCEi Search...

Gene expression databases

Bgeei Q8VCR8.
Genevestigatori Q8VCR8.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-591.
    Strain: C57BL/6J.
    Tissue: Bone.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 374-613.
    Tissue: Salivary gland.

Entry informationi

Entry nameiMYLK2_MOUSE
AccessioniPrimary (citable) accession number: Q8VCR8
Secondary accession number(s): A2APB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: July 10, 2007
Last modified: July 9, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi