Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q8VCR8

- MYLK2_MOUSE

UniProt

Q8VCR8 - MYLK2_MOUSE

Protein

Myosin light chain kinase 2, skeletal/cardiac muscle

Gene

Mylk2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 2 (10 Jul 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain By similarity.By similarity

    Catalytic activityi

    ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei331 – 3311ATPPROSITE-ProRule annotation
    Active sitei423 – 4231Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi308 – 3169ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. myosin light chain kinase activity Source: UniProtKB-EC
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. cardiac muscle cell differentiation Source: UniProtKB
    2. cardiac muscle tissue morphogenesis Source: Ensembl
    3. neuromuscular synaptic transmission Source: MGI
    4. peptidyl-threonine phosphorylation Source: Ensembl
    5. positive regulation of gene expression Source: Ensembl
    6. protein autophosphorylation Source: Ensembl
    7. regulation of muscle filament sliding Source: Ensembl
    8. skeletal muscle cell differentiation Source: UniProtKB
    9. skeletal muscle satellite cell differentiation Source: UniProtKB
    10. striated muscle contraction Source: Ensembl

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
    Short name:
    MLCK2
    Gene namesi
    Name:Mylk2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:2139434. Mylk2.

    Subcellular locationi

    Cytoplasm By similarity
    Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 613612Myosin light chain kinase 2, skeletal/cardiac musclePRO_0000086409Add
    BLAST

    Proteomic databases

    MaxQBiQ8VCR8.
    PaxDbiQ8VCR8.
    PRIDEiQ8VCR8.

    PTM databases

    PhosphoSiteiQ8VCR8.

    Expressioni

    Gene expression databases

    BgeeiQ8VCR8.
    GenevestigatoriQ8VCR8.

    Interactioni

    Subunit structurei

    May interact with centrin.By similarity

    Protein-protein interaction databases

    IntActiQ8VCR8. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCR8.
    SMRiQ8VCR8. Positions 217-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini302 – 557256Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni591 – 60313Calmodulin-bindingBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi96 – 1038Poly-Gly
    Compositional biasi278 – 2858Poly-Pro

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117629.
    HOGENOMiHOG000233016.
    HOVERGENiHBG080416.
    KOiK00907.
    OMAiCFQILXG.
    OrthoDBiEOG73FQMV.
    TreeFamiTF314166.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q8VCR8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTENGAVEL GSQSLSTEQT PKAAAGDGPS ASEKEPSAPA TEKDLSPPNA    50
    KKDPGAPDPK NNPDPPSLKK DPAKAPGPEK KGDPVPASAS SQGPSGEGDG 100
    GGGPAEGSEG PPAALPLPTA TAEASIQKLD PTQAPSGNQG SGEAKAGKKA 150
    AECREAGRRG SPAFLHSPSC PAIISCSEKT LAVKPLSETT DLVFTGVSVT 200
    PDPQDPGPVK AGGTNALAEK KKEEAEKASG QAGQAKVQGD TPQRIGFQAV 250
    PSERVEVGQA LCLTAREEDC FQILDDCPPP PAPFPHRIVE LRTGNVNSEF 300
    SMNSKEALGG GKFGAVCTCT ERATGLKLAA KVIKKQTPKD KEMVLLEIEV 350
    MNQLNHRNLI QLYAAIETSH EIILFMEYIE GGELFERIVD EDYHLTEVDT 400
    MVFVRQICDG ILFMHKMRVL HLDLKPENIL CVNTTGHLVK IIDFGLARRY 450
    NPNEKLKVNF GTPEFLSPEV VNYDQISDKT DMWSLGVITY MLLSGLSPFL 500
    GDDDTETLNN VLSANWYFDE ETFEAVSDEA KDFVSNLLTK DQSARMSAEQ 550
    CLAHPWLNNL AEKAKRCNRR LKSQILLKKY LMKRRWKKNF IAVSAANRFK 600
    KISSSGALMA LGV 613
    Length:613
    Mass (Da):65,990
    Last modified:July 10, 2007 - v2
    Checksum:i1FAC64C8B09DAE25
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL833801 Genomic DNA. Translation: CAM23678.1.
    AK079396 mRNA. No translation available.
    BC019408 mRNA. Translation: AAH19408.1.
    CCDSiCCDS38282.2.
    RefSeqiNP_001074513.2. NM_001081044.2.
    UniGeneiMm.250604.

    Genome annotation databases

    EnsembliENSMUST00000028970; ENSMUSP00000028970; ENSMUSG00000027470.
    GeneIDi228785.
    KEGGimmu:228785.
    UCSCiuc008ngs.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL833801 Genomic DNA. Translation: CAM23678.1 .
    AK079396 mRNA. No translation available.
    BC019408 mRNA. Translation: AAH19408.1 .
    CCDSi CCDS38282.2.
    RefSeqi NP_001074513.2. NM_001081044.2.
    UniGenei Mm.250604.

    3D structure databases

    ProteinModelPortali Q8VCR8.
    SMRi Q8VCR8. Positions 217-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VCR8. 2 interactions.

    PTM databases

    PhosphoSitei Q8VCR8.

    Proteomic databases

    MaxQBi Q8VCR8.
    PaxDbi Q8VCR8.
    PRIDEi Q8VCR8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028970 ; ENSMUSP00000028970 ; ENSMUSG00000027470 .
    GeneIDi 228785.
    KEGGi mmu:228785.
    UCSCi uc008ngs.2. mouse.

    Organism-specific databases

    CTDi 85366.
    MGIi MGI:2139434. Mylk2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117629.
    HOGENOMi HOG000233016.
    HOVERGENi HBG080416.
    KOi K00907.
    OMAi CFQILXG.
    OrthoDBi EOG73FQMV.
    TreeFami TF314166.

    Miscellaneous databases

    ChiTaRSi MYLK2. mouse.
    NextBioi 379136.
    PROi Q8VCR8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VCR8.
    Genevestigatori Q8VCR8.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-591.
      Strain: C57BL/6J.
      Tissue: Bone.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 374-613.
      Tissue: Salivary gland.

    Entry informationi

    Entry nameiMYLK2_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCR8
    Secondary accession number(s): A2APB9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2002
    Last sequence update: July 10, 2007
    Last modified: October 1, 2014
    This is version 110 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3