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Protein

Myosin light chain kinase 2, skeletal/cardiac muscle

Gene

Mylk2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain (By similarity).By similarity

Catalytic activityi

ATP + [myosin light-chain] = ADP + [myosin light-chain] phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei331 – 3311ATPPROSITE-ProRule annotation
Active sitei423 – 4231Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi308 – 3169ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cardiac muscle cell differentiation Source: UniProtKB
  • cardiac muscle tissue morphogenesis Source: MGI
  • neuromuscular synaptic transmission Source: MGI
  • peptidyl-threonine phosphorylation Source: MGI
  • positive regulation of gene expression Source: MGI
  • protein autophosphorylation Source: MGI
  • regulation of muscle filament sliding Source: MGI
  • skeletal muscle cell differentiation Source: UniProtKB
  • skeletal muscle satellite cell differentiation Source: UniProtKB
  • striated muscle contraction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain kinase 2, skeletal/cardiac muscle (EC:2.7.11.18)
Short name:
MLCK2
Gene namesi
Name:Mylk2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:2139434. Mylk2.

Subcellular locationi

  • Cytoplasm By similarity

  • Note: Colocalizes with phosphorylated myosin light chain (RLCP) at filaments of the myofibrils.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 613612Myosin light chain kinase 2, skeletal/cardiac musclePRO_0000086409Add
BLAST

Proteomic databases

MaxQBiQ8VCR8.
PaxDbiQ8VCR8.
PRIDEiQ8VCR8.

PTM databases

PhosphoSiteiQ8VCR8.

Expressioni

Gene expression databases

BgeeiQ8VCR8.
GenevisibleiQ8VCR8. MM.

Interactioni

Subunit structurei

May interact with centrin.By similarity

Protein-protein interaction databases

IntActiQ8VCR8. 2 interactions.
STRINGi10090.ENSMUSP00000028970.

Structurei

3D structure databases

ProteinModelPortaliQ8VCR8.
SMRiQ8VCR8. Positions 217-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini302 – 557256Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni591 – 60313Calmodulin-bindingBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi96 – 1038Poly-Gly
Compositional biasi278 – 2858Poly-Pro

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118877.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
InParanoidiQ8VCR8.
KOiK00907.
OMAiYVMKRRW.
OrthoDBiEOG73FQMV.
TreeFamiTF314166.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VCR8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTENGAVEL GSQSLSTEQT PKAAAGDGPS ASEKEPSAPA TEKDLSPPNA
60 70 80 90 100
KKDPGAPDPK NNPDPPSLKK DPAKAPGPEK KGDPVPASAS SQGPSGEGDG
110 120 130 140 150
GGGPAEGSEG PPAALPLPTA TAEASIQKLD PTQAPSGNQG SGEAKAGKKA
160 170 180 190 200
AECREAGRRG SPAFLHSPSC PAIISCSEKT LAVKPLSETT DLVFTGVSVT
210 220 230 240 250
PDPQDPGPVK AGGTNALAEK KKEEAEKASG QAGQAKVQGD TPQRIGFQAV
260 270 280 290 300
PSERVEVGQA LCLTAREEDC FQILDDCPPP PAPFPHRIVE LRTGNVNSEF
310 320 330 340 350
SMNSKEALGG GKFGAVCTCT ERATGLKLAA KVIKKQTPKD KEMVLLEIEV
360 370 380 390 400
MNQLNHRNLI QLYAAIETSH EIILFMEYIE GGELFERIVD EDYHLTEVDT
410 420 430 440 450
MVFVRQICDG ILFMHKMRVL HLDLKPENIL CVNTTGHLVK IIDFGLARRY
460 470 480 490 500
NPNEKLKVNF GTPEFLSPEV VNYDQISDKT DMWSLGVITY MLLSGLSPFL
510 520 530 540 550
GDDDTETLNN VLSANWYFDE ETFEAVSDEA KDFVSNLLTK DQSARMSAEQ
560 570 580 590 600
CLAHPWLNNL AEKAKRCNRR LKSQILLKKY LMKRRWKKNF IAVSAANRFK
610
KISSSGALMA LGV
Length:613
Mass (Da):65,990
Last modified:July 10, 2007 - v2
Checksum:i1FAC64C8B09DAE25
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL833801 Genomic DNA. Translation: CAM23678.1.
AK079396 mRNA. No translation available.
BC019408 mRNA. Translation: AAH19408.1.
CCDSiCCDS38282.2.
RefSeqiNP_001074513.2. NM_001081044.2.
UniGeneiMm.250604.

Genome annotation databases

EnsembliENSMUST00000028970; ENSMUSP00000028970; ENSMUSG00000027470.
GeneIDi228785.
KEGGimmu:228785.
UCSCiuc008ngs.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL833801 Genomic DNA. Translation: CAM23678.1.
AK079396 mRNA. No translation available.
BC019408 mRNA. Translation: AAH19408.1.
CCDSiCCDS38282.2.
RefSeqiNP_001074513.2. NM_001081044.2.
UniGeneiMm.250604.

3D structure databases

ProteinModelPortaliQ8VCR8.
SMRiQ8VCR8. Positions 217-608.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCR8. 2 interactions.
STRINGi10090.ENSMUSP00000028970.

PTM databases

PhosphoSiteiQ8VCR8.

Proteomic databases

MaxQBiQ8VCR8.
PaxDbiQ8VCR8.
PRIDEiQ8VCR8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028970; ENSMUSP00000028970; ENSMUSG00000027470.
GeneIDi228785.
KEGGimmu:228785.
UCSCiuc008ngs.2. mouse.

Organism-specific databases

CTDi85366.
MGIiMGI:2139434. Mylk2.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000118877.
HOGENOMiHOG000233016.
HOVERGENiHBG080416.
InParanoidiQ8VCR8.
KOiK00907.
OMAiYVMKRRW.
OrthoDBiEOG73FQMV.
TreeFamiTF314166.

Miscellaneous databases

ChiTaRSiMylk2. mouse.
NextBioi379136.
PROiQ8VCR8.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCR8.
GenevisibleiQ8VCR8. MM.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-591.
    Strain: C57BL/6J.
    Tissue: Bone.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 374-613.
    Tissue: Salivary gland.

Entry informationi

Entry nameiMYLK2_MOUSE
AccessioniPrimary (citable) accession number: Q8VCR8
Secondary accession number(s): A2APB9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: July 10, 2007
Last modified: July 22, 2015
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.