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Protein

Nuclear receptor-binding factor 2

Gene

Nrbf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May modulate transcriptional activation by target nuclear receptors. Can act as transcriptional activator (in vitro) (By similarity).By similarity
Involved in starvation-induced autophagy probably by its association with PI3K complex I (PI3KC3-C1). However, effects has been described variably. Involved in the induction of starvation-induced autophagy (By similarity). Stabilzes PI3KC3-C1 assembly and enhances ATG14-linked lipid kinase activity of PIK3C3 (PubMed:24849286). Proposed to negatively regulate basal and starvation-induced autophagy and to inhibit PIK3C3 activity by modulating interactions in PI3KC3-C1 (By similarity). May be involved in autophagosome biogenesis (By similarity). May play a role in neural progenitor cell survival during differentiation (PubMed:18619852).By similarity2 Publications
Involved in the induction of starvation-induced autophagy. Modulates ATG14-linked lipid kinase activity of PIK3C3 and stabilzes PI3K complex I (PI3KC3-C1) assembly (PubMed:24849286). May play a role in neural progenitor cell survival during differentiation (PubMed:18619852).By similarity2 Publications

GO - Molecular functioni

GO - Biological processi

  • autophagy Source: UniProtKB-KW
  • regulation of autophagy Source: UniProtKB
  • regulation of lipid kinase activity Source: UniProtKB
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to endoplasmic reticulum stress Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Autophagy, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear receptor-binding factor 2
Short name:
NRBF-2
Gene namesi
Name:Nrbf2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1354950. Nrbf2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Nuclear receptor-binding factor 2PRO_0000235817Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei268 – 2681PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ8VCQ3.
MaxQBiQ8VCQ3.
PaxDbiQ8VCQ3.
PRIDEiQ8VCQ3.

PTM databases

iPTMnetiQ8VCQ3.
PhosphoSiteiQ8VCQ3.

Expressioni

Developmental stagei

During embryonic brain development detected in the cerebral cortex at E14; expressed in the walls of the third and fourth ventricles, and in the hippocampus. In the adult brain, predominantly expressed in Purkinje cells of the cerebellum and neurons in the CA3 region of the hippocampus (PubMed:18619852).1 Publication

Gene expression databases

BgeeiQ8VCQ3.
CleanExiMM_NRBF2.
ExpressionAtlasiQ8VCQ3. baseline.
GenevisibleiQ8VCQ3. MM.

Interactioni

Subunit structurei

Interacts with RRARA, PPARD and PPARG. Interacts with THRB, RARA, RARG and RXRA in the presence of bound ligand (By similarity). Interacts with SCOC (By similarity). Associates with the PI3K complex I (PI3KC3-C1); the direct binding partner in the complex is reported variably as PIK3R4 or ATG14 (PubMed:24849286).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Atg14Q8CDJ37EBI-2365563,EBI-3506699
Becn1O885975EBI-2365563,EBI-643716

GO - Molecular functioni

Protein-protein interaction databases

BioGridi565935. 1 interaction.
IntActiQ8VCQ3. 8 interactions.
STRINGi10090.ENSMUSP00000097254.

Structurei

Secondary structure

1
287
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2317Combined sources
Helixi27 – 4519Combined sources
Helixi51 – 8535Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CRBNMR-A4-87[»]
ProteinModelPortaliQ8VCQ3.
SMRiQ8VCQ3. Positions 4-87.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ8VCQ3.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili168 – 21548Sequence analysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi141 – 1455Nuclear receptor interaction motifBy similarity

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IJBN. Eukaryota.
ENOG4111SDN. LUCA.
GeneTreeiENSGT00390000000984.
HOGENOMiHOG000293418.
HOVERGENiHBG056976.
InParanoidiQ8VCQ3.
OMAiTWQKFAS.
OrthoDBiEOG7XDBH8.
PhylomeDBiQ8VCQ3.
TreeFamiTF328627.

Family and domain databases

InterProiIPR015056. NRBF2.
IPR033393. NRBF2_MIT.
[Graphical view]
PfamiPF08961. NRBF2. 1 hit.
PF17169. NRBF2_MIT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCQ3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVMEGPLNL AHQQSRRADR LLAAGKYEEA ISCHRKATTY LSEAMKLTES
60 70 80 90 100
EQAHLSLELQ RDSHMKQLLL IQERWKRAKR EERLKAQQST ERDGAPHLQA
110 120 130 140 150
PPRPSEDAEG QSPLLSQPYI PSTERRLPEV QGVFDRDPDT LLFLLQQKNE
160 170 180 190 200
PSEPCIGSKA PKDDKTIIEE QATKIADLKR HVEFLVAENE RLRKENKQLK
210 220 230 240 250
AEKARLLKGT AEKELDVDAD FVEKSELWGL PSHSESAAAS STWQKFAANT
260 270 280
GKAKDIPIPN LPPLDFPSPE LPLMELSEDI LKGFMND
Length:287
Mass (Da):32,501
Last modified:March 1, 2002 - v1
Checksum:i81B1C0A0CB575D2E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti203 – 2031K → E in BAB22375 (PubMed:16141072).Curated
Sequence conflicti245 – 26420KFAAN…NLPPL → SLQLTRGSQGHSNTQPSSS in BAB22375 (PubMed:16141072).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002810 mRNA. Translation: BAB22375.1.
BC019448 mRNA. Translation: AAH19448.1.
CCDSiCCDS23902.1.
RefSeqiNP_001031370.1. NM_001036293.2.
UniGeneiMm.136223.

Genome annotation databases

EnsembliENSMUST00000077839; ENSMUSP00000097254; ENSMUSG00000075000.
GeneIDi641340.
KEGGimmu:641340.
UCSCiuc007flw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002810 mRNA. Translation: BAB22375.1.
BC019448 mRNA. Translation: AAH19448.1.
CCDSiCCDS23902.1.
RefSeqiNP_001031370.1. NM_001036293.2.
UniGeneiMm.136223.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2CRBNMR-A4-87[»]
ProteinModelPortaliQ8VCQ3.
SMRiQ8VCQ3. Positions 4-87.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi565935. 1 interaction.
IntActiQ8VCQ3. 8 interactions.
STRINGi10090.ENSMUSP00000097254.

PTM databases

iPTMnetiQ8VCQ3.
PhosphoSiteiQ8VCQ3.

Proteomic databases

EPDiQ8VCQ3.
MaxQBiQ8VCQ3.
PaxDbiQ8VCQ3.
PRIDEiQ8VCQ3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000077839; ENSMUSP00000097254; ENSMUSG00000075000.
GeneIDi641340.
KEGGimmu:641340.
UCSCiuc007flw.1. mouse.

Organism-specific databases

CTDi29982.
MGIiMGI:1354950. Nrbf2.

Phylogenomic databases

eggNOGiENOG410IJBN. Eukaryota.
ENOG4111SDN. LUCA.
GeneTreeiENSGT00390000000984.
HOGENOMiHOG000293418.
HOVERGENiHBG056976.
InParanoidiQ8VCQ3.
OMAiTWQKFAS.
OrthoDBiEOG7XDBH8.
PhylomeDBiQ8VCQ3.
TreeFamiTF328627.

Enzyme and pathway databases

ReactomeiR-MMU-383280. Nuclear Receptor transcription pathway.

Miscellaneous databases

EvolutionaryTraceiQ8VCQ3.
PROiQ8VCQ3.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCQ3.
CleanExiMM_NRBF2.
ExpressionAtlasiQ8VCQ3. baseline.
GenevisibleiQ8VCQ3. MM.

Family and domain databases

InterProiIPR015056. NRBF2.
IPR033393. NRBF2_MIT.
[Graphical view]
PfamiPF08961. NRBF2. 1 hit.
PF17169. NRBF2_MIT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. "Nuclear receptor binding protein 2 is induced during neural progenitor differentiation and affects cell survival."
    Larsson J., Forsberg M., Brannvall K., Zhang X.Q., Enarsson M., Hedborg F., Forsberg-Nilsson K.
    Mol. Cell. Neurosci. 39:32-39(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  4. "NRBF2 regulates autophagy and prevents liver injury by modulating Atg14L-linked phosphatidylinositol-3 kinase III activity."
    Lu J., He L., Behrends C., Araki M., Araki K., Jun Wang Q., Catanzaro J.M., Friedman S.L., Zong W.X., Fiel M.I., Li M., Yue Z.
    Nat. Commun. 5:3920-3920(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ATG14, SUBUNIT.
  5. "Solution structure of MIT domain from mouse Nrbf-2."
    RIKEN structural genomics initiative (RSGI)
    Submitted (NOV-2005) to the PDB data bank
    Cited for: STRUCTURE BY NMR OF 4-86.

Entry informationi

Entry nameiNRBF2_MOUSE
AccessioniPrimary (citable) accession number: Q8VCQ3
Secondary accession number(s): Q9DCG3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 16, 2006
Last sequence update: March 1, 2002
Last modified: June 8, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.