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Protein

NADPH oxidase organizer 1

Gene

Noxo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.2 Publications

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-5668599. RHO GTPases Activate NADPH Oxidases.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH oxidase organizer 1
Short name:
Nox organizer 1
Alternative name(s):
Sorting nexin-28
Gene namesi
Name:Noxo1
Synonyms:Snx28
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1919143. Noxo1.

Subcellular locationi

  • Cell membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity

  • Note: Associates with the plasma membrane in a lipid-dependent manner.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice display balance defects being unable to orient themselves with respect to the gravitational force. This is associated with a defect in otoconia biogenesis in the inner ear.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002275951 – 349NADPH oxidase organizer 1Add BLAST349

Proteomic databases

PaxDbiQ8VCM2.
PRIDEiQ8VCM2.

PTM databases

PhosphoSitePlusiQ8VCM2.

Expressioni

Tissue specificityi

Strongly expressed by colon epithelial cells and to a lower extent in small intestine, uterus, stomach and testis. Expressed in different parts of the inner ear including sensory and nonsensory cell layers of the saccule, ampullae of the semicircular canals, the stria vascularis and the spiral glanglion neurons.3 Publications

Developmental stagei

Strongly expressed in inner ear during embryogenesis.1 Publication

Gene expression databases

BgeeiENSMUSG00000019320.
CleanExiMM_NOXO1.
GenevisibleiQ8VCM2. MM.

Interactioni

Subunit structurei

Interacts with NOX1, NOXA1, CYBA/p22phox and NCF2/p67phox. Interacts with SH3PXD2A and SH3PXD2B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi215012. 2 interactors.
STRINGi10090.ENSMUSP00000019464.

Structurei

3D structure databases

ProteinModelPortaliQ8VCM2.
SMRiQ8VCM2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 126PXPROSITE-ProRule annotationAdd BLAST126
Domaini157 – 219SH3 1PROSITE-ProRule annotationAdd BLAST63
Domaini231 – 290SH3 2PROSITE-ProRule annotationAdd BLAST60

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni315 – 324Proline-rich region; mediates mutually exclusive interactions with itself and NOXA1By similarity10

Domaini

The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation.By similarity
The SH3 domains mediate interaction with CYBA/p22phox.By similarity

Sequence similaritiesi

Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
Contains 2 SH3 domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH3 domain

Phylogenomic databases

eggNOGiENOG410IX9P. Eukaryota.
ENOG410ZVBX. LUCA.
GeneTreeiENSGT00530000063010.
HOGENOMiHOG000113833.
HOVERGENiHBG082053.
InParanoidiQ8VCM2.
KOiK17934.
OMAiTFVRRSW.
OrthoDBiEOG091G0HF0.
PhylomeDBiQ8VCM2.
TreeFamiTF329347.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VCM2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPRHPVSA HAVALVQMDR LQTFAFSVCW SDNSDTFVRR SWDEFRQLQK
60 70 80 90 100
TLKKTFPVEA GLLRRSEQVL PKLPDAPLLT RRGHTGRGLV RLRLLDTYVQ
110 120 130 140 150
ALLATSEHIL RSSALHGFFV PKPLDLEPML PPGSLVILPT PEEPLSQPRG
160 170 180 190 200
SLDIHSLEAQ SIPCVQPFHT LDIRDRPFHT KAQEILDILL RHPSGWWLVE
210 220 230 240 250
NKDQQVAWFP APYLEEVATC QGQESGLALQ GSGRQFCTTQ AYEGSRSDEL
260 270 280 290 300
SVPSGARVHV LETSDRGWWL CRYNGRTGLL PAMSLQPEGL GSLLGRPGFP
310 320 330 340
DSAGADKVAE DRTIPPVVPT RPCMSAIQSR CCSITRRALG QEQGTRVPR
Length:349
Mass (Da):38,827
Last modified:March 21, 2006 - v2
Checksum:i500F1E1247454101
GO
Isoform 2 (identifier: Q8VCM2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     234-239: RQFCTT → MSPSLL
     240-349: Missing.

Show »
Length:239
Mass (Da):26,844
Checksum:iE514D86B1CB07516
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13V → I in BAE34305 (PubMed:16141072).Curated1
Sequence conflicti195G → S in AAK94017 (Ref. 2) Curated1
Sequence conflicti195G → S in BAB26387 (PubMed:16141072).Curated1
Sequence conflicti239T → I in BAE34305 (PubMed:16141072).Curated1
Sequence conflicti340G → A in AAH19525 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017562234 – 239RQFCTT → MSPSLL in isoform 2. 2 Publications6
Alternative sequenceiVSP_017563240 – 349Missing in isoform 2. 2 PublicationsAdd BLAST110

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539797 mRNA. Translation: AAN75142.1.
AF399754 mRNA. Translation: AAK94017.1.
AK009605 mRNA. Translation: BAB26387.1.
AK088226 mRNA. Translation: BAC40222.1.
AK157993 mRNA. Translation: BAE34305.1.
BC019525 mRNA. Translation: AAH19525.1.
CCDSiCCDS50018.1. [Q8VCM2-1]
RefSeqiNP_082264.2. NM_027988.4. [Q8VCM2-1]
XP_006525012.1. XM_006524949.2. [Q8VCM2-1]
XP_011244931.1. XM_011246629.2. [Q8VCM2-1]
UniGeneiMm.390971.

Genome annotation databases

EnsembliENSMUST00000019464; ENSMUSP00000019464; ENSMUSG00000019320. [Q8VCM2-1]
GeneIDi71893.
KEGGimmu:71893.
UCSCiuc008axs.2. mouse. [Q8VCM2-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF539797 mRNA. Translation: AAN75142.1.
AF399754 mRNA. Translation: AAK94017.1.
AK009605 mRNA. Translation: BAB26387.1.
AK088226 mRNA. Translation: BAC40222.1.
AK157993 mRNA. Translation: BAE34305.1.
BC019525 mRNA. Translation: AAH19525.1.
CCDSiCCDS50018.1. [Q8VCM2-1]
RefSeqiNP_082264.2. NM_027988.4. [Q8VCM2-1]
XP_006525012.1. XM_006524949.2. [Q8VCM2-1]
XP_011244931.1. XM_011246629.2. [Q8VCM2-1]
UniGeneiMm.390971.

3D structure databases

ProteinModelPortaliQ8VCM2.
SMRiQ8VCM2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215012. 2 interactors.
STRINGi10090.ENSMUSP00000019464.

PTM databases

PhosphoSitePlusiQ8VCM2.

Proteomic databases

PaxDbiQ8VCM2.
PRIDEiQ8VCM2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000019464; ENSMUSP00000019464; ENSMUSG00000019320. [Q8VCM2-1]
GeneIDi71893.
KEGGimmu:71893.
UCSCiuc008axs.2. mouse. [Q8VCM2-1]

Organism-specific databases

CTDi124056.
MGIiMGI:1919143. Noxo1.

Phylogenomic databases

eggNOGiENOG410IX9P. Eukaryota.
ENOG410ZVBX. LUCA.
GeneTreeiENSGT00530000063010.
HOGENOMiHOG000113833.
HOVERGENiHBG082053.
InParanoidiQ8VCM2.
KOiK17934.
OMAiTFVRRSW.
OrthoDBiEOG091G0HF0.
PhylomeDBiQ8VCM2.
TreeFamiTF329347.

Enzyme and pathway databases

ReactomeiR-MMU-5668599. RHO GTPases Activate NADPH Oxidases.

Miscellaneous databases

PROiQ8VCM2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019320.
CleanExiMM_NOXO1.
GenevisibleiQ8VCM2. MM.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamiPF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 2 hits.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNOXO1_MOUSE
AccessioniPrimary (citable) accession number: Q8VCM2
Secondary accession number(s): Q3TZA4, Q8BH41, Q9D747
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: November 30, 2016
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.