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Q8VCM2

- NOXO1_MOUSE

UniProt

Q8VCM2 - NOXO1_MOUSE

Protein

NADPH oxidase organizer 1

Gene

Noxo1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (21 Mar 2006)
      Previous versions | rss
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    Functioni

    Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity.2 Publications

    GO - Molecular functioni

    1. phosphatidylinositol binding Source: InterPro
    2. superoxide-generating NADPH oxidase activator activity Source: MGI

    GO - Biological processi

    1. extracellular matrix disassembly Source: UniProtKB
    2. positive regulation of catalytic activity Source: GOC
    3. superoxide metabolic process Source: MGI

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADPH oxidase organizer 1
    Short name:
    Nox organizer 1
    Alternative name(s):
    Sorting nexin-28
    Gene namesi
    Name:Noxo1
    Synonyms:Snx28
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1919143. Noxo1.

    Subcellular locationi

    Cell membrane By similarity
    Note: Associates with the plasma membrane in a lipid-dependent manner.By similarity

    GO - Cellular componenti

    1. NADPH oxidase complex Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Mice display balance defects being unable to orient themselves with respect to the gravitational force. This is associated with a defect in otoconia biogenesis in the inner ear.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 349349NADPH oxidase organizer 1PRO_0000227595Add
    BLAST

    Proteomic databases

    PRIDEiQ8VCM2.

    PTM databases

    PhosphoSiteiQ8VCM2.

    Expressioni

    Tissue specificityi

    Strongly expressed by colon epithelial cells and to a lower extent in small intestine, uterus, stomach and testis. Expressed in different parts of the inner ear including sensory and nonsensory cell layers of the saccule, ampullae of the semicircular canals, the stria vascularis and the spiral glanglion neurons.3 Publications

    Developmental stagei

    Strongly expressed in inner ear during embryogenesis.1 Publication

    Gene expression databases

    ArrayExpressiQ8VCM2.
    BgeeiQ8VCM2.
    CleanExiMM_NOXO1.
    GenevestigatoriQ8VCM2.

    Interactioni

    Subunit structurei

    Interacts with NOX1, NOXA1, CYBA/p22phox and NCF2/p67phox. Interacts with SH3PXD2A and SH3PXD2B.By similarity

    Protein-protein interaction databases

    BioGridi215012. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCM2.
    SMRiQ8VCM2. Positions 2-143, 184-309.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 126126PXPROSITE-ProRule annotationAdd
    BLAST
    Domaini157 – 21963SH3 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini231 – 29060SH3 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni315 – 32410Proline-rich region; mediates mutually exclusive interactions with itself and NOXA1By similarity

    Domaini

    The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation.By similarity
    The SH3 domains mediate interaction with CYBA/p22phox.By similarity

    Sequence similaritiesi

    Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation
    Contains 2 SH3 domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH3 domain

    Phylogenomic databases

    eggNOGiNOG150477.
    GeneTreeiENSGT00530000063010.
    HOGENOMiHOG000113833.
    HOVERGENiHBG082053.
    InParanoidiQ8VCM2.
    KOiK17934.
    OMAiTFVRRSW.
    OrthoDBiEOG70GMG8.
    PhylomeDBiQ8VCM2.
    TreeFamiTF329347.

    Family and domain databases

    Gene3Di3.30.1520.10. 1 hit.
    InterProiIPR001683. Phox.
    IPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00787. PX. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00312. PX. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 2 hits.
    SSF64268. SSF64268. 1 hit.
    PROSITEiPS50195. PX. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VCM2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MASPRHPVSA HAVALVQMDR LQTFAFSVCW SDNSDTFVRR SWDEFRQLQK    50
    TLKKTFPVEA GLLRRSEQVL PKLPDAPLLT RRGHTGRGLV RLRLLDTYVQ 100
    ALLATSEHIL RSSALHGFFV PKPLDLEPML PPGSLVILPT PEEPLSQPRG 150
    SLDIHSLEAQ SIPCVQPFHT LDIRDRPFHT KAQEILDILL RHPSGWWLVE 200
    NKDQQVAWFP APYLEEVATC QGQESGLALQ GSGRQFCTTQ AYEGSRSDEL 250
    SVPSGARVHV LETSDRGWWL CRYNGRTGLL PAMSLQPEGL GSLLGRPGFP 300
    DSAGADKVAE DRTIPPVVPT RPCMSAIQSR CCSITRRALG QEQGTRVPR 349
    Length:349
    Mass (Da):38,827
    Last modified:March 21, 2006 - v2
    Checksum:i500F1E1247454101
    GO
    Isoform 2 (identifier: Q8VCM2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         234-239: RQFCTT → MSPSLL
         240-349: Missing.

    Show »
    Length:239
    Mass (Da):26,844
    Checksum:iE514D86B1CB07516
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti13 – 131V → I in BAE34305. (PubMed:16141072)Curated
    Sequence conflicti195 – 1951G → S in AAK94017. 1 PublicationCurated
    Sequence conflicti195 – 1951G → S in BAB26387. (PubMed:16141072)Curated
    Sequence conflicti239 – 2391T → I in BAE34305. (PubMed:16141072)Curated
    Sequence conflicti340 – 3401G → A in AAH19525. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei234 – 2396RQFCTT → MSPSLL in isoform 2. 2 PublicationsVSP_017562
    Alternative sequencei240 – 349110Missing in isoform 2. 2 PublicationsVSP_017563Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF539797 mRNA. Translation: AAN75142.1.
    AF399754 mRNA. Translation: AAK94017.1.
    AK009605 mRNA. Translation: BAB26387.1.
    AK088226 mRNA. Translation: BAC40222.1.
    AK157993 mRNA. Translation: BAE34305.1.
    BC019525 mRNA. Translation: AAH19525.1.
    CCDSiCCDS50018.1. [Q8VCM2-1]
    RefSeqiNP_082264.2. NM_027988.4. [Q8VCM2-1]
    XP_006525012.1. XM_006524949.1. [Q8VCM2-1]
    XP_006525013.1. XM_006524950.1. [Q8VCM2-1]
    UniGeneiMm.390971.

    Genome annotation databases

    EnsembliENSMUST00000019464; ENSMUSP00000019464; ENSMUSG00000019320. [Q8VCM2-1]
    GeneIDi71893.
    KEGGimmu:71893.
    UCSCiuc008axs.2. mouse. [Q8VCM2-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF539797 mRNA. Translation: AAN75142.1 .
    AF399754 mRNA. Translation: AAK94017.1 .
    AK009605 mRNA. Translation: BAB26387.1 .
    AK088226 mRNA. Translation: BAC40222.1 .
    AK157993 mRNA. Translation: BAE34305.1 .
    BC019525 mRNA. Translation: AAH19525.1 .
    CCDSi CCDS50018.1. [Q8VCM2-1 ]
    RefSeqi NP_082264.2. NM_027988.4. [Q8VCM2-1 ]
    XP_006525012.1. XM_006524949.1. [Q8VCM2-1 ]
    XP_006525013.1. XM_006524950.1. [Q8VCM2-1 ]
    UniGenei Mm.390971.

    3D structure databases

    ProteinModelPortali Q8VCM2.
    SMRi Q8VCM2. Positions 2-143, 184-309.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 215012. 2 interactions.

    PTM databases

    PhosphoSitei Q8VCM2.

    Proteomic databases

    PRIDEi Q8VCM2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000019464 ; ENSMUSP00000019464 ; ENSMUSG00000019320 . [Q8VCM2-1 ]
    GeneIDi 71893.
    KEGGi mmu:71893.
    UCSCi uc008axs.2. mouse. [Q8VCM2-1 ]

    Organism-specific databases

    CTDi 124056.
    MGIi MGI:1919143. Noxo1.

    Phylogenomic databases

    eggNOGi NOG150477.
    GeneTreei ENSGT00530000063010.
    HOGENOMi HOG000113833.
    HOVERGENi HBG082053.
    InParanoidi Q8VCM2.
    KOi K17934.
    OMAi TFVRRSW.
    OrthoDBi EOG70GMG8.
    PhylomeDBi Q8VCM2.
    TreeFami TF329347.

    Miscellaneous databases

    NextBioi 334872.
    PROi Q8VCM2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VCM2.
    Bgeei Q8VCM2.
    CleanExi MM_NOXO1.
    Genevestigatori Q8VCM2.

    Family and domain databases

    Gene3Di 3.30.1520.10. 1 hit.
    InterProi IPR001683. Phox.
    IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00787. PX. 1 hit.
    PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00312. PX. 1 hit.
    SM00326. SH3. 2 hits.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 2 hits.
    SSF64268. SSF64268. 1 hit.
    PROSITEi PS50195. PX. 1 hit.
    PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Two novel proteins activate superoxide generation by the NADPH oxidase NOX1."
      Banfi B., Clark R.A., Steger K., Krause K.-H.
      J. Biol. Chem. 278:3510-3513(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Colon.
    2. Hong W.
      Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
      Strain: C57BL/6J and NOD.
      Tissue: Inner ear and Thymus.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Colon.
    5. "Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3."
      Cheng G., Lambeth J.D.
      Gene 356:118-126(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. "Inactivation of NADPH oxidase organizer 1 results in severe imbalance."
      Kiss P.J., Knisz J., Zhang Y., Baltrusaitis J., Sigmund C.D., Thalmann R., Smith R.J.H., Verpy E., Banfi B.
      Curr. Biol. 16:208-213(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiNOXO1_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCM2
    Secondary accession number(s): Q3TZA4, Q8BH41, Q9D747
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 21, 2006
    Last sequence update: March 21, 2006
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3