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Q8VCM2 (NOXO1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADPH oxidase organizer 1

Short name=Nox organizer 1
Alternative name(s):
Sorting nexin-28
Gene names
Name:Noxo1
Synonyms:Snx28
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length349 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity. Ref.1 Ref.6

Subunit structure

Interacts with NOX1, NOXA1, CYBA/p22phox and NCF2/p67phox By similarity. Interacts with SH3PXD2A and SH3PXD2B By similarity.

Subcellular location

Cell membrane By similarity. Note: Associates with the plasma membrane in a lipid-dependent manner By similarity.

Tissue specificity

Strongly expressed by colon epithelial cells and to a lower extent in small intestine, uterus, stomach and testis. Expressed in different parts of the inner ear including sensory and nonsensory cell layers of the saccule, ampullae of the semicircular canals, the stria vascularis and the spiral glanglion neurons. Ref.1 Ref.5 Ref.6

Developmental stage

Strongly expressed in inner ear during embryogenesis. Ref.6

Domain

The PX domain mediates lipid-binding, localization to the plasma membrane and is required for NOX1 activation By similarity.

The SH3 domains mediate interaction with CYBA/p22phox By similarity.

Disruption phenotype

Mice display balance defects being unable to orient themselves with respect to the gravitational force. This is associated with a defect in otoconia biogenesis in the inner ear. Ref.6

Sequence similarities

Contains 1 PX (phox homology) domain.

Contains 2 SH3 domains.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q8VCM2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q8VCM2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     234-239: RQFCTT → MSPSLL
     240-349: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 349349NADPH oxidase organizer 1
PRO_0000227595

Regions

Domain1 – 126126PX
Domain157 – 21963SH3 1
Domain231 – 29060SH3 2
Region315 – 32410Proline-rich region; mediates mutually exclusive interactions with itself and NOXA1 By similarity

Natural variations

Alternative sequence234 – 2396RQFCTT → MSPSLL in isoform 2.
VSP_017562
Alternative sequence240 – 349110Missing in isoform 2.
VSP_017563

Experimental info

Sequence conflict131V → I in BAE34305. Ref.3
Sequence conflict1951G → S in AAK94017. Ref.2
Sequence conflict1951G → S in BAB26387. Ref.3
Sequence conflict2391T → I in BAE34305. Ref.3
Sequence conflict3401G → A in AAH19525. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified March 21, 2006. Version 2.
Checksum: 500F1E1247454101

FASTA34938,827
        10         20         30         40         50         60 
MASPRHPVSA HAVALVQMDR LQTFAFSVCW SDNSDTFVRR SWDEFRQLQK TLKKTFPVEA 

        70         80         90        100        110        120 
GLLRRSEQVL PKLPDAPLLT RRGHTGRGLV RLRLLDTYVQ ALLATSEHIL RSSALHGFFV 

       130        140        150        160        170        180 
PKPLDLEPML PPGSLVILPT PEEPLSQPRG SLDIHSLEAQ SIPCVQPFHT LDIRDRPFHT 

       190        200        210        220        230        240 
KAQEILDILL RHPSGWWLVE NKDQQVAWFP APYLEEVATC QGQESGLALQ GSGRQFCTTQ 

       250        260        270        280        290        300 
AYEGSRSDEL SVPSGARVHV LETSDRGWWL CRYNGRTGLL PAMSLQPEGL GSLLGRPGFP 

       310        320        330        340 
DSAGADKVAE DRTIPPVVPT RPCMSAIQSR CCSITRRALG QEQGTRVPR 

« Hide

Isoform 2 [UniParc].

Checksum: E514D86B1CB07516
Show »

FASTA23926,844

References

« Hide 'large scale' references
[1]"Two novel proteins activate superoxide generation by the NADPH oxidase NOX1."
Banfi B., Clark R.A., Steger K., Krause K.-H.
J. Biol. Chem. 278:3510-3513(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Colon.
[2]Hong W.
Submitted (JUL-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Strain: C57BL/6J.
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: C57BL/6J and NOD.
Tissue: Inner ear and Thymus.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Colon.
[5]"Alternative mRNA splice forms of NOXO1: differential tissue expression and regulation of Nox1 and Nox3."
Cheng G., Lambeth J.D.
Gene 356:118-126(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Inactivation of NADPH oxidase organizer 1 results in severe imbalance."
Kiss P.J., Knisz J., Zhang Y., Baltrusaitis J., Sigmund C.D., Thalmann R., Smith R.J.H., Verpy E., Banfi B.
Curr. Biol. 16:208-213(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF539797 mRNA. Translation: AAN75142.1.
AF399754 mRNA. Translation: AAK94017.1.
AK009605 mRNA. Translation: BAB26387.1.
AK088226 mRNA. Translation: BAC40222.1.
AK157993 mRNA. Translation: BAE34305.1.
BC019525 mRNA. Translation: AAH19525.1.
CCDSCCDS50018.1. [Q8VCM2-1]
RefSeqNP_082264.2. NM_027988.4. [Q8VCM2-1]
XP_006525012.1. XM_006524949.1. [Q8VCM2-1]
XP_006525013.1. XM_006524950.1. [Q8VCM2-1]
UniGeneMm.390971.

3D structure databases

ProteinModelPortalQ8VCM2.
SMRQ8VCM2. Positions 2-143, 184-309.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid215012. 2 interactions.

PTM databases

PhosphoSiteQ8VCM2.

Proteomic databases

PRIDEQ8VCM2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000019464; ENSMUSP00000019464; ENSMUSG00000019320. [Q8VCM2-1]
GeneID71893.
KEGGmmu:71893.
UCSCuc008axs.2. mouse. [Q8VCM2-1]

Organism-specific databases

CTD124056.
MGIMGI:1919143. Noxo1.

Phylogenomic databases

eggNOGNOG150477.
GeneTreeENSGT00530000063010.
HOGENOMHOG000113833.
HOVERGENHBG082053.
InParanoidQ8VCM2.
KOK17934.
OMATFVRRSW.
OrthoDBEOG70GMG8.
PhylomeDBQ8VCM2.
TreeFamTF329347.

Gene expression databases

ArrayExpressQ8VCM2.
BgeeQ8VCM2.
CleanExMM_NOXO1.
GenevestigatorQ8VCM2.

Family and domain databases

Gene3D3.30.1520.10. 1 hit.
InterProIPR001683. Phox.
IPR001452. SH3_domain.
[Graphical view]
PfamPF00787. PX. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00312. PX. 1 hit.
SM00326. SH3. 2 hits.
[Graphical view]
SUPFAMSSF50044. SSF50044. 2 hits.
SSF64268. SSF64268. 1 hit.
PROSITEPS50195. PX. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio334872.
PROQ8VCM2.
SOURCESearch...

Entry information

Entry nameNOXO1_MOUSE
AccessionPrimary (citable) accession number: Q8VCM2
Secondary accession number(s): Q3TZA4, Q8BH41, Q9D747
Entry history
Integrated into UniProtKB/Swiss-Prot: March 21, 2006
Last sequence update: March 21, 2006
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot