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Protein

Kelch-like protein 20

Gene

Klhl20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport, Ubl conjugation pathway

Keywords - Ligandi

Actin-binding

Enzyme and pathway databases

ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 20
Alternative name(s):
Kelch-like ECT2-interacting protein
Gene namesi
Name:Klhl20
Synonyms:Kiaa4210, Kleip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2444855. Klhl20.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus, Nucleus

Pathology & Biotechi

Disruption phenotypei

Approximately 50% of mice die until day 28 of postnatal development (P28). Mice that survive beyond P28 are indistinguishable at birth, but show a progressive corneal dystrophy, associated with an epithelial hyperplasia and an altered corneal epithelial cell differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Kelch-like protein 20PRO_0000119124Add
BLAST

Proteomic databases

MaxQBiQ8VCK5.
PaxDbiQ8VCK5.
PRIDEiQ8VCK5.

PTM databases

iPTMnetiQ8VCK5.
PhosphoSiteiQ8VCK5.

Expressioni

Gene expression databases

BgeeiQ8VCK5.
CleanExiMM_KLHL20.
ExpressionAtlasiQ8VCK5. baseline and differential.
GenevisibleiQ8VCK5. MM.

Interactioni

Subunit structurei

Component of the BCR(KLHL20) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL20 and RBX1. Interacts with PDZ-RhoGEF/ARHGEF11, DAPK1 and CORO7. Interacts with F-actin. Interacts with IFN-gamma (IFNG) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107238.

Structurei

3D structure databases

ProteinModelPortaliQ8VCK5.
SMRiQ8VCK5. Positions 45-594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini63 – 13068BTBPROSITE-ProRule annotationAdd
BLAST
Domaini165 – 267103BACKAdd
BLAST
Repeati314 – 36047Kelch 1Add
BLAST
Repeati362 – 40847Kelch 2Add
BLAST
Repeati409 – 45547Kelch 3Add
BLAST
Repeati457 – 50246Kelch 4Add
BLAST
Repeati504 – 54946Kelch 5Add
BLAST
Repeati551 – 59646Kelch 6Add
BLAST

Sequence similaritiesi

Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 6 Kelch repeats.Curated

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118931.
HOGENOMiHOG000230814.
HOVERGENiHBG014286.
InParanoidiQ8VCK5.
KOiK10457.
OMAiENRWHTV.
OrthoDBiEOG7ZWD17.
PhylomeDBiQ8VCK5.
TreeFamiTF329218.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR015916. Gal_Oxidase_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF50965. SSF50965. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRCTNIRPG ETGMDVTSRC TLGDPNKLPE GVPQPARMPY ISDKHPRQTL
60 70 80 90 100
EVINLLRKHR ELCDVVLVVG AKKIYAHRVI LSACSPYFRA MFTGELAESR
110 120 130 140 150
QTEVVIRDID ERAMELLIDF AYTSQITVEE GNVQTLLPAA CLLQLAEIQE
160 170 180 190 200
ACCEFLKRQL DPSNCLGIRA FADTHSCREL LRIADKFTQH NFQEVMESEE
210 220 230 240 250
FMLLPANQLI DIISSDELNV RSEEQVFNAV MAWVKYSIQE RRPQLPQVLQ
260 270 280 290 300
HVRLPLLSPK FLVGTVGSDP LIKSDEECRD LVDEAKNYLL LPQERPLMQG
310 320 330 340 350
PRTRPRKPIR CGEVLFAVGG WCSGDAISSV ERYDPQTNEW RMVASMSKRR
360 370 380 390 400
CGVGVSVLDD LLYAVGGHDG SSYLNSVERY DPKTNQWSSD VAPTSTCRTS
410 420 430 440 450
VGVAVLGGFL YAVGGQDGVS CLNIVERYDP KENKWTRVAS MSTRRLGVAV
460 470 480 490 500
AVLGGFLYAV GGSDGTSPLN TVERYNPQEN RWHTIAPMGT RRKHLGCAVY
510 520 530 540 550
QDMIYAVGGR DDTTELSSAE RYNPRTNQWS PVVAMTSRRS GVGLAVVNGQ
560 570 580 590 600
LMAVGGFDGT TYLKTIEVFD PDANTWRLYG GMNYRRLGGG VGVIKMTHCE

SHIW
Length:604
Mass (Da):67,412
Last modified:March 1, 2004 - v2
Checksum:i3A87B3DC714B1BC9
GO

Sequence cautioni

The sequence BAD90319.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220547 mRNA. Translation: BAD90319.1. Different initiation.
AK053102 mRNA. Translation: BAC35266.1.
BC019571 mRNA. Translation: AAH19571.2.
CCDSiCCDS35748.1.
RefSeqiNP_001034571.1. NM_001039482.1.
UniGeneiMm.255165.

Genome annotation databases

EnsembliENSMUST00000111611; ENSMUSP00000107238; ENSMUSG00000026705.
ENSMUST00000117467; ENSMUSP00000114044; ENSMUSG00000026705.
GeneIDi226541.
KEGGimmu:226541.
UCSCiuc007dff.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220547 mRNA. Translation: BAD90319.1. Different initiation.
AK053102 mRNA. Translation: BAC35266.1.
BC019571 mRNA. Translation: AAH19571.2.
CCDSiCCDS35748.1.
RefSeqiNP_001034571.1. NM_001039482.1.
UniGeneiMm.255165.

3D structure databases

ProteinModelPortaliQ8VCK5.
SMRiQ8VCK5. Positions 45-594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107238.

PTM databases

iPTMnetiQ8VCK5.
PhosphoSiteiQ8VCK5.

Proteomic databases

MaxQBiQ8VCK5.
PaxDbiQ8VCK5.
PRIDEiQ8VCK5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000111611; ENSMUSP00000107238; ENSMUSG00000026705.
ENSMUST00000117467; ENSMUSP00000114044; ENSMUSG00000026705.
GeneIDi226541.
KEGGimmu:226541.
UCSCiuc007dff.1. mouse.

Organism-specific databases

CTDi27252.
MGIiMGI:2444855. Klhl20.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4441. Eukaryota.
ENOG410XNX8. LUCA.
GeneTreeiENSGT00760000118931.
HOGENOMiHOG000230814.
HOVERGENiHBG014286.
InParanoidiQ8VCK5.
KOiK10457.
OMAiENRWHTV.
OrthoDBiEOG7ZWD17.
PhylomeDBiQ8VCK5.
TreeFamiTF329218.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

NextBioi378218.
PROiQ8VCK5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCK5.
CleanExiMM_KLHL20.
ExpressionAtlasiQ8VCK5. baseline and differential.
GenevisibleiQ8VCK5. MM.

Family and domain databases

Gene3Di2.130.10.80. 1 hit.
InterProiIPR011705. BACK.
IPR017096. BTB-kelch_protein.
IPR000210. BTB/POZ_dom.
IPR011043. Gal_Oxase/kelch_b-propeller.
IPR015916. Gal_Oxidase_b-propeller.
IPR006652. Kelch_1.
IPR011333. SKP1/BTB/POZ.
[Graphical view]
PfamiPF07707. BACK. 1 hit.
PF00651. BTB. 1 hit.
PF01344. Kelch_1. 6 hits.
[Graphical view]
PIRSFiPIRSF037037. Kelch-like_protein_gigaxonin. 1 hit.
SMARTiSM00875. BACK. 1 hit.
SM00225. BTB. 1 hit.
SM00612. Kelch. 6 hits.
[Graphical view]
SUPFAMiSSF50965. SSF50965. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Fetal brain.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Head.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  4. "KLEIP deficiency in mice causes progressive corneal neovascular dystrophy."
    Hahn N., Dietz C.T., Kuhl S., Vossmerbaeumer U., Kroll J.
    Invest. Ophthalmol. Vis. Sci. 53:3260-3268(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiKLH20_MOUSE
AccessioniPrimary (citable) accession number: Q8VCK5
Secondary accession number(s): Q5DTH3, Q8BWA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2004
Last modified: May 11, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.