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Protein

Kelch-like protein 20

Gene

Klhl20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis. The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination. Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking. Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (By similarity).By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processProtein transport, Transport, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-8951664 Neddylation
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayiUPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Kelch-like protein 20
Alternative name(s):
Kelch-like ECT2-interacting protein
Gene namesi
Name:Klhl20
Synonyms:Kiaa4210, Kleip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:2444855 Klhl20

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Cytoplasm, Golgi apparatus, Nucleus

Pathology & Biotechi

Disruption phenotypei

Approximately 50% of mice die until day 28 of postnatal development (P28). Mice that survive beyond P28 are indistinguishable at birth, but show a progressive corneal dystrophy, associated with an epithelial hyperplasia and an altered corneal epithelial cell differentiation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001191241 – 604Kelch-like protein 20Add BLAST604

Proteomic databases

MaxQBiQ8VCK5
PaxDbiQ8VCK5
PRIDEiQ8VCK5

PTM databases

iPTMnetiQ8VCK5
PhosphoSitePlusiQ8VCK5

Expressioni

Gene expression databases

BgeeiENSMUSG00000026705
CleanExiMM_KLHL20
ExpressionAtlasiQ8VCK5 baseline and differential
GenevisibleiQ8VCK5 MM

Interactioni

Subunit structurei

Component of the BCR(KLHL20) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL20 and RBX1. Interacts with PDZ-RhoGEF/ARHGEF11, DAPK1, PML and CORO7. Interacts with F-actin. Interacts with IFN-gamma (IFNG) (By similarity). Interacts (via kelch repeats) with IVNS1ABP (via kelch repeats); this interaction blocks the assembly of CUL3-KLHL20 complex (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000107238

Structurei

3D structure databases

ProteinModelPortaliQ8VCK5
SMRiQ8VCK5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini63 – 130BTBPROSITE-ProRule annotationAdd BLAST68
Domaini165 – 267BACKAdd BLAST103
Repeati314 – 360Kelch 1Add BLAST47
Repeati362 – 408Kelch 2Add BLAST47
Repeati409 – 455Kelch 3Add BLAST47
Repeati457 – 502Kelch 4Add BLAST46
Repeati504 – 549Kelch 5Add BLAST46
Repeati551 – 596Kelch 6Add BLAST46

Keywords - Domaini

Kelch repeat, Repeat

Phylogenomic databases

eggNOGiKOG4441 Eukaryota
ENOG410XNX8 LUCA
GeneTreeiENSGT00760000118931
HOGENOMiHOG000230814
HOVERGENiHBG014286
InParanoidiQ8VCK5
KOiK10457
OMAiNSWSPIV
OrthoDBiEOG091G04QJ
PhylomeDBiQ8VCK5
TreeFamiTF329218

Family and domain databases

Gene3Di2.120.10.80, 2 hits
InterProiView protein in InterPro
IPR011705 BACK
IPR017096 BTB-kelch_protein
IPR000210 BTB/POZ_dom
IPR011043 Gal_Oxase/kelch_b-propeller
IPR015915 Kelch-typ_b-propeller
IPR006652 Kelch_1
IPR011333 SKP1/BTB/POZ_sf
PfamiView protein in Pfam
PF07707 BACK, 1 hit
PF00651 BTB, 1 hit
PF01344 Kelch_1, 6 hits
PIRSFiPIRSF037037 Kelch-like_protein_gigaxonin, 1 hit
SMARTiView protein in SMART
SM00875 BACK, 1 hit
SM00225 BTB, 1 hit
SM00612 Kelch, 6 hits
SUPFAMiSSF50965 SSF50965, 1 hit
SSF54695 SSF54695, 1 hit
PROSITEiView protein in PROSITE
PS50097 BTB, 1 hit

Sequencei

Sequence statusi: Complete.

Q8VCK5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRRCTNIRPG ETGMDVTSRC TLGDPNKLPE GVPQPARMPY ISDKHPRQTL
60 70 80 90 100
EVINLLRKHR ELCDVVLVVG AKKIYAHRVI LSACSPYFRA MFTGELAESR
110 120 130 140 150
QTEVVIRDID ERAMELLIDF AYTSQITVEE GNVQTLLPAA CLLQLAEIQE
160 170 180 190 200
ACCEFLKRQL DPSNCLGIRA FADTHSCREL LRIADKFTQH NFQEVMESEE
210 220 230 240 250
FMLLPANQLI DIISSDELNV RSEEQVFNAV MAWVKYSIQE RRPQLPQVLQ
260 270 280 290 300
HVRLPLLSPK FLVGTVGSDP LIKSDEECRD LVDEAKNYLL LPQERPLMQG
310 320 330 340 350
PRTRPRKPIR CGEVLFAVGG WCSGDAISSV ERYDPQTNEW RMVASMSKRR
360 370 380 390 400
CGVGVSVLDD LLYAVGGHDG SSYLNSVERY DPKTNQWSSD VAPTSTCRTS
410 420 430 440 450
VGVAVLGGFL YAVGGQDGVS CLNIVERYDP KENKWTRVAS MSTRRLGVAV
460 470 480 490 500
AVLGGFLYAV GGSDGTSPLN TVERYNPQEN RWHTIAPMGT RRKHLGCAVY
510 520 530 540 550
QDMIYAVGGR DDTTELSSAE RYNPRTNQWS PVVAMTSRRS GVGLAVVNGQ
560 570 580 590 600
LMAVGGFDGT TYLKTIEVFD PDANTWRLYG GMNYRRLGGG VGVIKMTHCE

SHIW
Length:604
Mass (Da):67,412
Last modified:March 1, 2004 - v2
Checksum:i3A87B3DC714B1BC9
GO

Sequence cautioni

The sequence BAD90319 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK220547 mRNA Translation: BAD90319.1 Different initiation.
AK053102 mRNA Translation: BAC35266.1
BC019571 mRNA Translation: AAH19571.2
CCDSiCCDS35748.1
RefSeqiNP_001034571.1, NM_001039482.1
UniGeneiMm.255165

Genome annotation databases

EnsembliENSMUST00000111611; ENSMUSP00000107238; ENSMUSG00000026705
ENSMUST00000117467; ENSMUSP00000114044; ENSMUSG00000026705
GeneIDi226541
KEGGimmu:226541
UCSCiuc007dff.1 mouse

Similar proteinsi

Entry informationi

Entry nameiKLH20_MOUSE
AccessioniPrimary (citable) accession number: Q8VCK5
Secondary accession number(s): Q5DTH3, Q8BWA2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: March 1, 2004
Last modified: May 23, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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