Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

3-ketoacyl-CoA thiolase B, peroxisomal

Gene

Acaa1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.

Pathwayi: fatty acid metabolism

This protein is involved in the pathway fatty acid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway fatty acid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei123 – 1231Acyl-thioester intermediateBy similarity
Active sitei377 – 3771Proton acceptorPROSITE-ProRule annotation
Active sitei408 – 4081Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Enzyme and pathway databases

BRENDAi2.3.1.16. 3474.
ReactomeiR-MMU-2046106. alpha-linolenic acid (ALA) metabolism.
R-MMU-390247. Beta-oxidation of very long chain fatty acids.
UniPathwayiUPA00199.

Names & Taxonomyi

Protein namesi
Recommended name:
3-ketoacyl-CoA thiolase B, peroxisomal (EC:2.3.1.16)
Alternative name(s):
Acetyl-CoA acyltransferase B
Beta-ketothiolase B
Peroxisomal 3-oxoacyl-CoA thiolase B
Gene namesi
Name:Acaa1b
Synonyms:Acaa1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:3605455. Acaa1b.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • peroxisome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2626PeroxisomeBy similarityAdd
BLAST
Chaini27 – 4243983-ketoacyl-CoA thiolase B, peroxisomalPRO_0000034069Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei173 – 1731N6-acetyllysineCombined sources
Modified residuei234 – 2341N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ8VCH0.
MaxQBiQ8VCH0.
PaxDbiQ8VCH0.
PRIDEiQ8VCH0.

PTM databases

iPTMnetiQ8VCH0.
PhosphoSiteiQ8VCH0.
SwissPalmiQ8VCH0.

Expressioni

Tissue specificityi

Mainly expressed in liver; weaker levels in kidney, intestine and white adipose tissue.1 Publication

Gene expression databases

BgeeiQ8VCH0.
ExpressionAtlasiQ8VCH0. baseline and differential.
GenevisibleiQ8VCH0. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiQ8VCH0. 5 interactions.
MINTiMINT-1858235.
STRINGi10090.ENSMUSP00000010795.

Structurei

3D structure databases

ProteinModelPortaliQ8VCH0.
SMRiQ8VCH0. Positions 32-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thiolase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1389. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00760000119318.
HOGENOMiHOG000012239.
HOVERGENiHBG003112.
InParanoidiQ8VCH0.
KOiK07513.
OMAiIQACLQV.
OrthoDBiEOG7K3TM6.
PhylomeDBiQ8VCH0.
TreeFamiTF332308.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q8VCH0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHRLQVVLGH LAGRPESSSA LQAAPCSAGF LQASASDVVV VHGRRTPIGR
60 70 80 90 100
ASRGCFKDTT PDELLSAVLT AVLQDVKLKP EQLGDISVGN VLQPGAGAIM
110 120 130 140 150
ARIAQFLSGI PETVPLSTVN RQCSSGLQAV ANIAGGIRNG SYDIGMACGV
160 170 180 190 200
ESMTLSQRGN HGNISSRLLE NEKARDCLIP MGITSENVAE RFGVSRQKQD
210 220 230 240 250
AFALASQQKA ASAQSRGCFH AEIVPVTTTV LNDKGDKKTI TVSQDEGVRP
260 270 280 290 300
STTMQGLAKL KPAFKDGGST TAGNSSQVSD GAAAVLLARR SKAEELGLPI
310 320 330 340 350
LGVLRSYAVV GVPPDVMGIG PAYAIPAALQ KAGLTVNDID IFEINEAFAS
360 370 380 390 400
QAVYCVEKLG IPAEKVNPLG GAIALGHPLG CTGARQVVTL LNELKRRGRR
410 420
AYGVVSMCIG TGMGAAAVFE YPGN
Length:424
Mass (Da):43,995
Last modified:March 1, 2002 - v1
Checksum:i29BFFFF02DD43BAC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY273812 mRNA. Translation: AAP31669.1.
BC019882 mRNA. Translation: AAH19882.1.
CCDSiCCDS23608.1.
RefSeqiNP_666342.1. NM_146230.3.
UniGeneiMm.379402.

Genome annotation databases

EnsembliENSMUST00000010795; ENSMUSP00000010795; ENSMUSG00000010651.
GeneIDi235674.
KEGGimmu:235674.
UCSCiuc009saj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY273812 mRNA. Translation: AAP31669.1.
BC019882 mRNA. Translation: AAH19882.1.
CCDSiCCDS23608.1.
RefSeqiNP_666342.1. NM_146230.3.
UniGeneiMm.379402.

3D structure databases

ProteinModelPortaliQ8VCH0.
SMRiQ8VCH0. Positions 32-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ8VCH0. 5 interactions.
MINTiMINT-1858235.
STRINGi10090.ENSMUSP00000010795.

PTM databases

iPTMnetiQ8VCH0.
PhosphoSiteiQ8VCH0.
SwissPalmiQ8VCH0.

Proteomic databases

EPDiQ8VCH0.
MaxQBiQ8VCH0.
PaxDbiQ8VCH0.
PRIDEiQ8VCH0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000010795; ENSMUSP00000010795; ENSMUSG00000010651.
GeneIDi235674.
KEGGimmu:235674.
UCSCiuc009saj.1. mouse.

Organism-specific databases

CTDi235674.
MGIiMGI:3605455. Acaa1b.

Phylogenomic databases

eggNOGiKOG1389. Eukaryota.
COG0183. LUCA.
GeneTreeiENSGT00760000119318.
HOGENOMiHOG000012239.
HOVERGENiHBG003112.
InParanoidiQ8VCH0.
KOiK07513.
OMAiIQACLQV.
OrthoDBiEOG7K3TM6.
PhylomeDBiQ8VCH0.
TreeFamiTF332308.

Enzyme and pathway databases

UniPathwayiUPA00199.
BRENDAi2.3.1.16. 3474.
ReactomeiR-MMU-2046106. alpha-linolenic acid (ALA) metabolism.
R-MMU-390247. Beta-oxidation of very long chain fatty acids.

Miscellaneous databases

ChiTaRSiAcaa1b. mouse.
NextBioi382837.
PROiQ8VCH0.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCH0.
ExpressionAtlasiQ8VCH0. baseline and differential.
GenevisibleiQ8VCH0. MM.

Family and domain databases

Gene3Di3.40.47.10. 4 hits.
InterProiIPR002155. Thiolase.
IPR016039. Thiolase-like.
IPR020615. Thiolase_acyl_enz_int_AS.
IPR020610. Thiolase_AS.
IPR020617. Thiolase_C.
IPR020613. Thiolase_CS.
IPR020616. Thiolase_N.
[Graphical view]
PfamiPF02803. Thiolase_C. 1 hit.
PF00108. Thiolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITEiPS00098. THIOLASE_1. 1 hit.
PS00737. THIOLASE_2. 1 hit.
PS00099. THIOLASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, gene structure and expression profile of two mouse peroxisomal 3-ketoacyl-CoA thiolase genes."
    Chevillard G., Clemencet M.-C., Etienne P., Martin P., Pineau T., Latruffe N., Nicolas-Frances V.
    BMC Biochem. 5:3-3(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  3. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney and Liver.
  4. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-173, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiTHIKB_MOUSE
AccessioniPrimary (citable) accession number: Q8VCH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2005
Last sequence update: March 1, 2002
Last modified: March 16, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.