Q8VCH0 (THIKB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-ketoacyl-CoA thiolase B, peroxisomal
EC=2.3.1.16

Alternative name(s):
Acetyl-CoA acyltransferase B
Beta-ketothiolase B
Peroxisomal 3-oxoacyl-CoA thiolase B

Gene names

Name:	Acaa1b
Synonyms:	Acaa1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA.
Pathway	Lipid metabolism; fatty acid metabolism.
Subunit structure	Homodimer By similarity.
Subcellular location	Peroxisome By similarity.
Tissue specificity	Mainly expressed in liver; weaker levels in kidney, intestine and white adipose tissue. Ref.1
Sequence similarities	Belongs to the thiolase family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Peroxisome
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid metabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrion Inferred from direct assay PubMed 18614015. Source: MGI peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	acetyl-CoA C-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 26	26	Peroxisome By similarity
	Chain	27 – 424	398	3-ketoacyl-CoA thiolase B, peroxisomal		PRO_0000034069
Sites
	Active site	123	1	Acyl-thioester intermediate By similarity
	Active site	377	1	Proton acceptor By similarity
	Active site	408	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8VCH0 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 29BFFFF02DD43BAC	FASTA	424	43,995
	10 20 30 40 50 60 MHRLQVVLGH LAGRPESSSA LQAAPCSAGF LQASASDVVV VHGRRTPIGR ASRGCFKDTT 70 80 90 100 110 120 PDELLSAVLT AVLQDVKLKP EQLGDISVGN VLQPGAGAIM ARIAQFLSGI PETVPLSTVN 130 140 150 160 170 180 RQCSSGLQAV ANIAGGIRNG SYDIGMACGV ESMTLSQRGN HGNISSRLLE NEKARDCLIP 190 200 210 220 230 240 MGITSENVAE RFGVSRQKQD AFALASQQKA ASAQSRGCFH AEIVPVTTTV LNDKGDKKTI 250 260 270 280 290 300 TVSQDEGVRP STTMQGLAKL KPAFKDGGST TAGNSSQVSD GAAAVLLARR SKAEELGLPI 310 320 330 340 350 360 LGVLRSYAVV GVPPDVMGIG PAYAIPAALQ KAGLTVNDID IFEINEAFAS QAVYCVEKLG 370 380 390 400 410 420 IPAEKVNPLG GAIALGHPLG CTGARQVVTL LNELKRRGRR AYGVVSMCIG TGMGAAAVFE YPGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, gene structure and expression profile of two mouse peroxisomal 3-ketoacyl-CoA thiolase genes." Chevillard G., Clemencet M.-C., Etienne P., Martin P., Pineau T., Latruffe N., Nicolas-Frances V. BMC Biochem. 5:3-3(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: C57BL/6J. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY273812 mRNA. Translation: AAP31669.1. BC019882 mRNA. Translation: AAH19882.1.
IPI	IPI00122139.
RefSeq	NP_666342.1. NM_146230.3.
UniGene	Mm.379402.
3D structure databases
ProteinModelPortal	Q8VCH0.
SMR	Q8VCH0. Positions 32-424.
ModBase	Search...
PTM databases
PhosphoSite	Q8VCH0.
Proteomic databases
PaxDb	Q8VCH0.
PRIDE	Q8VCH0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000010795; ENSMUSP00000010795; ENSMUSG00000010651.
GeneID	235674.
KEGG	mmu:235674.
Organism-specific databases
CTD	235674.
MGI	MGI:3605455. Acaa1b.
Phylogenomic databases
eggNOG	COG0183.
GeneTree	ENSGT00620000087956.
HOGENOM	HOG000012239.
HOVERGEN	HBG003112.
InParanoid	Q8VCH0.
KO	K07513.
OMA	WAGDPET.
OrthoDB	EOG45QHDC.
Enzyme and pathway databases
BRENDA	2.3.1.16. 3474.
UniPathway	UPA00199.
Gene expression databases
ArrayExpress	Q8VCH0.
Bgee	Q8VCH0.
Genevestigator	Q8VCH0.
GermOnline	ENSMUSG00000010651. Mus musculus.
Family and domain databases
Gene3D	3.40.47.10. 4 hits.
InterPro	IPR002155. Thiolase. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. IPR020615. Thiolase_acyl_enz_int_AS. IPR020610. Thiolase_AS. IPR020617. Thiolase_C. IPR020613. Thiolase_CS. IPR020616. Thiolase_N. [Graphical view]
PANTHER	PTHR18919. PTHR18919. 1 hit.
Pfam	PF02803. Thiolase_C. 1 hit. PF00108. Thiolase_N. 1 hit. [Graphical view]
PIRSF	PIRSF000429. Ac-CoA_Ac_transf. 1 hit.
SUPFAM	SSF53901. Thiolase-like. 2 hits.
TIGRFAMs	TIGR01930. AcCoA-C-Actrans. 1 hit.
PROSITE	PS00098. THIOLASE_1. 1 hit. PS00737. THIOLASE_2. 1 hit. PS00099. THIOLASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	Acaa1b. mouse.
NextBio	382837.
SOURCE	Search...

Entry information

Entry name

THIKB_MOUSE

Accession

Primary (citable) accession number: Q8VCH0

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program