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Q8VCD5 (MED17_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mediator of RNA polymerase II transcription subunit 17
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 6
Short name=CRSP complex subunit 6
Mediator complex subunit 17
Thyroid hormone receptor-associated protein complex 80 kDa component
Gene names
Name:Med17
Synonyms:Crsp6, Trap80
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length649 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors By similarity.

Subunit structure

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with STAT2 By similarity. Interacts with GATA1 and PPARG. Ref.3 Ref.4

Subcellular location

Nucleus Probable.

Sequence similarities

Belongs to the Mediator complex subunit 17 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 649649Mediator of RNA polymerase II transcription subunit 17
PRO_0000304700

Experimental info

Sequence conflict5501S → G in BAC30005. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q8VCD5 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 924D5C2D4150B4B5

FASTA64972,462
        10         20         30         40         50         60 
MSGVRAVRIS IESACEKQVQ EVGLDGTETY LQPLSMSQNL ARLAQRIDFS QGSGSEEEEA 

        70         80         90        100        110        120 
AGPDGDAPDW GGAGADQDDE EGLVKFQPSL WPWDSVRNNL RSALTEMCVL YDVLSIVRDK 

       130        140        150        160        170        180 
KFMTLDPVSQ DALPPKQSPQ TLQLISKKKS LAGAAQILLK GAERLTKSVA ENQENKLQRD 

       190        200        210        220        230        240 
FNSELLRLRQ HWKLRKVGDK ILGDLSYRSA GSLFPHHGTF EVIKNTDIDL DKKIPEDYCP 

       250        260        270        280        290        300 
LDVQIPSDLE GSAYIKVSIQ KQAPDIGDLG TVNLFKRPLP KSKPGSPHWQ TKLEAAQNVL 

       310        320        330        340        350        360 
LCKEIFAQLS REAVQIKSQI PHIVVKNQII SQPFPSLQLS ISLCHSSDDK KSQKCAAEKP 

       370        380        390        400        410        420 
GQEDHLYVLE HNLHLLIREF HKQTLSSIVM PHPASAPFGH KRMRLSGPQA FDKNEINSIQ 

       430        440        450        460        470        480 
STEGLLEKII KQAKHIFLRS RTAATIDSLA SRIEDPQIQA HWSNINDVYE SSVKVLITSQ 

       490        500        510        520        530        540 
GYEQICKSIQ LQLNIGVEQV RVVHRDGRVI MLSHQEQELQ DFLLSQMSQH QVHAVQQLAK 

       550        560        570        580        590        600 
VMGWQVLSFS NHVGLGPIES IGNASAITVA SPSGDYAISV RNGPESGSKI MVQFPRNQCK 

       610        620        630        640 
DLPKSDVLQD SKWSHLRGPF KEVQWNKMEG RNFVYKMELL MSALSPCLL 

« Hide

References

« Hide 'large scale' references
[1]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Bone marrow, Hypothalamus and Testis.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Czech II.
Tissue: Eye and Mammary tumor.
[3]"Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis."
Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M., Roeder R.G.
Nature 417:563-567(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PPARG.
[4]"The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220."
Stumpf M., Waskow C., Kroetschel M., van Essen D., Rodriguez P., Zhang X., Guyot B., Roeder R.G., Borggrefe T.
Proc. Natl. Acad. Sci. U.S.A. 103:18504-18509(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GATA1, ASSOCIATION WITH PROMOTER REGIONS.
[5]Erratum
Stumpf M., Waskow C., Kroetschel M., van Essen D., Rodriguez P., Zhang X., Guyot B., Roeder R.G., Borggrefe T.
Proc. Natl. Acad. Sci. U.S.A. 104:1442-1442(2007)
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK033151 mRNA. Translation: BAC28172.1.
AK038451 mRNA. Translation: BAC30005.1.
AK049112 mRNA. Translation: BAC33548.1.
AK151377 mRNA. Translation: BAE30349.1.
BC004062 mRNA. Translation: AAH04062.1.
BC020185 mRNA. Translation: AAH20185.1.
RefSeqNP_659182.1. NM_144933.1.
UniGeneMm.44151.

3D structure databases

ProteinModelPortalQ8VCD5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid231602. 4 interactions.
DIPDIP-48407N.
IntActQ8VCD5. 5 interactions.
STRING10090.ENSMUSP00000034411.

PTM databases

PhosphoSiteQ8VCD5.

Proteomic databases

PaxDbQ8VCD5.
PRIDEQ8VCD5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000034411; ENSMUSP00000034411; ENSMUSG00000031935.
GeneID234959.
KEGGmmu:234959.
UCSCuc009ofl.1. mouse.

Organism-specific databases

CTD9440.
MGIMGI:2182585. Med17.

Phylogenomic databases

eggNOGNOG310166.
GeneTreeENSGT00390000011810.
HOGENOMHOG000008073.
HOVERGENHBG103247.
InParanoidQ8VCD5.
KOK15133.
OMAPPKQNPQ.
OrthoDBEOG7W9RT5.
PhylomeDBQ8VCD5.
TreeFamTF323615.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Gene expression databases

BgeeQ8VCD5.
CleanExMM_MED17.
GenevestigatorQ8VCD5.

Family and domain databases

InterProIPR019313. Mediator_Med17.
[Graphical view]
PfamPF10156. Med17. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio382444.
PROQ8VCD5.
SOURCESearch...

Entry information

Entry nameMED17_MOUSE
AccessionPrimary (citable) accession number: Q8VCD5
Secondary accession number(s): Q8BYS6, Q99KQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2002
Last modified: April 16, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot