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Protein

Mediator of RNA polymerase II transcription subunit 17

Gene

Med17

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

  1. receptor activity Source: MGI
  2. RNA polymerase II transcription cofactor activity Source: MGI
  3. thyroid hormone receptor binding Source: MGI
  4. transcription coactivator activity Source: MGI
  5. transcription cofactor activity Source: MGI

GO - Biological processi

  1. androgen receptor signaling pathway Source: MGI
  2. intracellular steroid hormone receptor signaling pathway Source: MGI
  3. positive regulation of transcription, DNA-templated Source: MGI
  4. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  5. regulation of transcription from RNA polymerase II promoter Source: MGI
  6. stem cell maintenance Source: MGI
  7. transcription initiation from RNA polymerase II promoter Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
REACT_275829. Generic Transcription Pathway.
REACT_309805. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 17
Alternative name(s):
Cofactor required for Sp1 transcriptional activation subunit 6
Short name:
CRSP complex subunit 6
Mediator complex subunit 17
Thyroid hormone receptor-associated protein complex 80 kDa component
Gene namesi
Name:Med17
Synonyms:Crsp6, Trap80
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:2182585. Med17.

Subcellular locationi

  1. Nucleus Curated

GO - Cellular componenti

  1. mediator complex Source: MGI
  2. membrane Source: MGI
  3. nucleoplasm Source: Reactome
  4. nucleus Source: MGI
  5. transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 649649Mediator of RNA polymerase II transcription subunit 17PRO_0000304700Add
BLAST

Proteomic databases

MaxQBiQ8VCD5.
PaxDbiQ8VCD5.
PRIDEiQ8VCD5.

PTM databases

PhosphoSiteiQ8VCD5.

Expressioni

Gene expression databases

BgeeiQ8VCD5.
CleanExiMM_MED17.
GenevestigatoriQ8VCD5.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with STAT2 (By similarity). Interacts with GATA1 and PPARG.By similarity2 Publications

Protein-protein interaction databases

BioGridi231602. 4 interactions.
DIPiDIP-48407N.
IntActiQ8VCD5. 5 interactions.
STRINGi10090.ENSMUSP00000034411.

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 17 family.Curated

Phylogenomic databases

eggNOGiNOG310166.
GeneTreeiENSGT00390000011810.
HOGENOMiHOG000008073.
HOVERGENiHBG103247.
InParanoidiQ8VCD5.
KOiK15133.
OMAiPPKQNPQ.
OrthoDBiEOG7W9RT5.
PhylomeDBiQ8VCD5.
TreeFamiTF323615.

Family and domain databases

InterProiIPR019313. Mediator_Med17.
[Graphical view]
PfamiPF10156. Med17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VCD5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGVRAVRIS IESACEKQVQ EVGLDGTETY LQPLSMSQNL ARLAQRIDFS
60 70 80 90 100
QGSGSEEEEA AGPDGDAPDW GGAGADQDDE EGLVKFQPSL WPWDSVRNNL
110 120 130 140 150
RSALTEMCVL YDVLSIVRDK KFMTLDPVSQ DALPPKQSPQ TLQLISKKKS
160 170 180 190 200
LAGAAQILLK GAERLTKSVA ENQENKLQRD FNSELLRLRQ HWKLRKVGDK
210 220 230 240 250
ILGDLSYRSA GSLFPHHGTF EVIKNTDIDL DKKIPEDYCP LDVQIPSDLE
260 270 280 290 300
GSAYIKVSIQ KQAPDIGDLG TVNLFKRPLP KSKPGSPHWQ TKLEAAQNVL
310 320 330 340 350
LCKEIFAQLS REAVQIKSQI PHIVVKNQII SQPFPSLQLS ISLCHSSDDK
360 370 380 390 400
KSQKCAAEKP GQEDHLYVLE HNLHLLIREF HKQTLSSIVM PHPASAPFGH
410 420 430 440 450
KRMRLSGPQA FDKNEINSIQ STEGLLEKII KQAKHIFLRS RTAATIDSLA
460 470 480 490 500
SRIEDPQIQA HWSNINDVYE SSVKVLITSQ GYEQICKSIQ LQLNIGVEQV
510 520 530 540 550
RVVHRDGRVI MLSHQEQELQ DFLLSQMSQH QVHAVQQLAK VMGWQVLSFS
560 570 580 590 600
NHVGLGPIES IGNASAITVA SPSGDYAISV RNGPESGSKI MVQFPRNQCK
610 620 630 640
DLPKSDVLQD SKWSHLRGPF KEVQWNKMEG RNFVYKMELL MSALSPCLL
Length:649
Mass (Da):72,462
Last modified:March 1, 2002 - v1
Checksum:i924D5C2D4150B4B5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti550 – 5501S → G in BAC30005 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033151 mRNA. Translation: BAC28172.1.
AK038451 mRNA. Translation: BAC30005.1.
AK049112 mRNA. Translation: BAC33548.1.
AK151377 mRNA. Translation: BAE30349.1.
BC004062 mRNA. Translation: AAH04062.1.
BC020185 mRNA. Translation: AAH20185.1.
CCDSiCCDS22834.1.
RefSeqiNP_659182.1. NM_144933.1.
UniGeneiMm.44151.

Genome annotation databases

EnsembliENSMUST00000034411; ENSMUSP00000034411; ENSMUSG00000031935.
GeneIDi234959.
KEGGimmu:234959.
UCSCiuc009ofl.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK033151 mRNA. Translation: BAC28172.1.
AK038451 mRNA. Translation: BAC30005.1.
AK049112 mRNA. Translation: BAC33548.1.
AK151377 mRNA. Translation: BAE30349.1.
BC004062 mRNA. Translation: AAH04062.1.
BC020185 mRNA. Translation: AAH20185.1.
CCDSiCCDS22834.1.
RefSeqiNP_659182.1. NM_144933.1.
UniGeneiMm.44151.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi231602. 4 interactions.
DIPiDIP-48407N.
IntActiQ8VCD5. 5 interactions.
STRINGi10090.ENSMUSP00000034411.

PTM databases

PhosphoSiteiQ8VCD5.

Proteomic databases

MaxQBiQ8VCD5.
PaxDbiQ8VCD5.
PRIDEiQ8VCD5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034411; ENSMUSP00000034411; ENSMUSG00000031935.
GeneIDi234959.
KEGGimmu:234959.
UCSCiuc009ofl.1. mouse.

Organism-specific databases

CTDi9440.
MGIiMGI:2182585. Med17.

Phylogenomic databases

eggNOGiNOG310166.
GeneTreeiENSGT00390000011810.
HOGENOMiHOG000008073.
HOVERGENiHBG103247.
InParanoidiQ8VCD5.
KOiK15133.
OMAiPPKQNPQ.
OrthoDBiEOG7W9RT5.
PhylomeDBiQ8VCD5.
TreeFamiTF323615.

Enzyme and pathway databases

ReactomeiREACT_27166. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.
REACT_275829. Generic Transcription Pathway.
REACT_309805. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

NextBioi382444.
PROiQ8VCD5.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VCD5.
CleanExiMM_MED17.
GenevestigatoriQ8VCD5.

Family and domain databases

InterProiIPR019313. Mediator_Med17.
[Graphical view]
PfamiPF10156. Med17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow, Hypothalamus and Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Eye and Mammary tumor.
  3. "Transcription coactivator TRAP220 is required for PPAR gamma 2-stimulated adipogenesis."
    Ge K., Guermah M., Yuan C.-X., Ito M., Wallberg A.E., Spiegelman B.M., Roeder R.G.
    Nature 417:563-567(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH PPARG.
  4. "The mediator complex functions as a coactivator for GATA-1 in erythropoiesis via subunit Med1/TRAP220."
    Stumpf M., Waskow C., Kroetschel M., van Essen D., Rodriguez P., Zhang X., Guyot B., Roeder R.G., Borggrefe T.
    Proc. Natl. Acad. Sci. U.S.A. 103:18504-18509(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GATA1, ASSOCIATION WITH PROMOTER REGIONS.
  5. Erratum
    Stumpf M., Waskow C., Kroetschel M., van Essen D., Rodriguez P., Zhang X., Guyot B., Roeder R.G., Borggrefe T.
    Proc. Natl. Acad. Sci. U.S.A. 104:1442-1442(2007)

Entry informationi

Entry nameiMED17_MOUSE
AccessioniPrimary (citable) accession number: Q8VCD5
Secondary accession number(s): Q8BYS6, Q99KQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: March 1, 2002
Last modified: April 1, 2015
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.