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Q8VCC8

- RPGF3_MOUSE

UniProt

Q8VCC8 - RPGF3_MOUSE

Protein

Rap guanine nucleotide exchange factor 3

Gene

Rapgef3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 2 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Guanine nucleotide exchange factor (GEF) for RAP1A and RAP2A small GTPases that is activated by binding cAMP. Through simultaneous binding of PDE3B to RAPGEF3 and PIK3R6 is assembled in a signaling complex in which it activates the PI3K gamma complex and which is involved in angiogenesis. Plays a role in the modulation of the cAMP-induced dynamic control of endothelial barrier function through a pathway that is independent on Rho-mediated signaling. Required for the actin rearrangement at cell-cell junctions, such as stress fibers and junctional actin By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi245 – 363119cAMPAdd
    BLAST

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. cAMP-dependent protein kinase regulator activity Source: InterPro
    3. guanyl-nucleotide exchange factor activity Source: MGI
    4. Rap guanyl-nucleotide exchange factor activity Source: UniProtKB

    GO - Biological processi

    1. angiogenesis Source: UniProtKB-KW
    2. cellular response to cAMP Source: UniProtKB
    3. establishment of endothelial barrier Source: UniProtKB
    4. positive regulation of angiogenesis Source: UniProtKB
    5. positive regulation of Rap GTPase activity Source: UniProtKB
    6. positive regulation of stress fiber assembly Source: UniProtKB
    7. Rap protein signal transduction Source: UniProtKB
    8. regulation of actin cytoskeleton reorganization Source: UniProtKB

    Keywords - Molecular functioni

    Guanine-nucleotide releasing factor

    Keywords - Biological processi

    Angiogenesis

    Keywords - Ligandi

    cAMP, cAMP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rap guanine nucleotide exchange factor 3
    Alternative name(s):
    Exchange factor directly activated by cAMP 1
    Exchange protein directly activated by cAMP 1
    Short name:
    EPAC 1
    cAMP-regulated guanine nucleotide exchange factor I
    Short name:
    cAMP-GEFI
    Gene namesi
    Name:Rapgef3
    Synonyms:Epac, Epac1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:2441741. Rapgef3.

    Subcellular locationi

    Cytoplasm. Membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. cAMP-dependent protein kinase complex Source: InterPro
    2. cytoplasm Source: UniProtKB-SubCell
    3. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 918918Rap guanine nucleotide exchange factor 3PRO_0000068868Add
    BLAST

    Proteomic databases

    PaxDbiQ8VCC8.
    PRIDEiQ8VCC8.

    PTM databases

    PhosphoSiteiQ8VCC8.

    Expressioni

    Gene expression databases

    ArrayExpressiQ8VCC8.
    BgeeiQ8VCC8.
    CleanExiMM_RAPGEF3.
    GenevestigatoriQ8VCC8.

    Interactioni

    Protein-protein interaction databases

    BioGridi230205. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCC8.
    SMRiQ8VCC8. Positions 89-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini110 – 18677DEPPROSITE-ProRule annotationAdd
    BLAST
    Domaini384 – 521138N-terminal Ras-GEFPROSITE-ProRule annotationAdd
    BLAST
    Domaini665 – 884220Ras-GEFPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 24225Interaction with PDE3BBy similarityAdd
    BLAST
    Regioni398 – 42225Interaction with PDE3BBy similarityAdd
    BLAST

    Domaini

    The DEP domain is involved in membrane localization independent from regulation by cAMP.By similarity

    Sequence similaritiesi

    Contains 1 cyclic nucleotide-binding domain.PROSITE-ProRule annotation
    Contains 1 DEP domain.PROSITE-ProRule annotation
    Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
    Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG236726.
    GeneTreeiENSGT00750000117452.
    HOGENOMiHOG000230545.
    HOVERGENiHBG056985.
    InParanoidiQ8VCC8.
    KOiK08014.
    OMAiNSFFAVM.
    OrthoDBiEOG7ZD1TF.
    PhylomeDBiQ8VCC8.
    TreeFamiTF313184.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.840.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProiIPR002373. cAMP/cGMP_kin.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR000591. DEP_dom.
    IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    IPR014710. RmlC-like_jellyroll.
    IPR029071. Ubiquitin-rel_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 1 hit.
    PF00610. DEP. 1 hit.
    PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    [Graphical view]
    PRINTSiPR00103. CAMPKINASE.
    SMARTiSM00100. cNMP. 1 hit.
    SM00049. DEP. 1 hit.
    SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48366. SSF48366. 3 hits.
    SSF51206. SSF51206. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
    PS50186. DEP. 1 hit.
    PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q8VCC8-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKVSWPGENH WQVGPAVVES PAVGAPQVGG LPDVVPEGTL LNMVLKRMHR    50
    PRCCSYQLVF EHRRPSCIQG LRWTPLTNSE DSLDFRVSLE QATTEHVHKA 100
    GKLLHRHLLA TYPTLIRDRK YHLRLYRHCC SGRELVDGIL ALGLGVHSRS 150
    QAVGICQVLL DEGALCHVKH DWTFQDRDAQ FYRFPGPEPE PTGTQDVEEE 200
    LVEAMALLSQ RGPDALLTVA LRKPPGQRTD EELDLIFEEL LHIKAVAHLS 250
    NSVKRELAAV LLFEPHSKAG TVLFSQGDKG TSWYIIWKGS VNVVTHGKGL 300
    VTTLHEGDDF GQLALVNDAP RAATIILREN NCHFLRVDKQ DFNRIIKDVE 350
    AKTMRLEEHG KVVLVLERTS QGAGPSRPPT PGRNRYTVMS GTPEKILELL 400
    LEAMRPDSSA HDPTETFLSD FLLTHSVFMP STQLFTALLH HFHVEPADPA 450
    GGSEQEHSTY ICNKRQQILR LVGRWVALYS PMLHSDPVAT SFLQKLSDLV 500
    SRDARLSNLL REQYPERRRH HRLENGCGNV SPQTKARNAP VWLPNQEEPL 550
    PSSAGAIRVG DKVPYDICRP DHSVLTLHLP VTASVREVMA ALAHEDHWTK 600
    GQVLVKVNSA GDVVGLQPDA RGVATSLGLN ERLFVVDPQE VHELTPHPEQ 650
    LGPTLGSSEM LDLVSAKDLA GQLTDHDWNL FNRIHQVQEH LRDVTTANLE 700
    RFMRRFNELQ YWVATELCLC PVPGSRAQLL RKFIKLAAHL KEQKNLNSFF 750
    AVMFGLSNSA ISRLAHTWER LPHKVRKLYS ALERLLDPSW NHRVYRLALT 800
    KLSPPVIPFM PLLLKDVTFI HEGNHTLVEN LINFEKMRMM ARAVRMLHHC 850
    RSHSTAPLSP LRSRVSHIHE DSQGSRISTC SEQSLSTRSP ASTWAYVQQL 900
    KVIDNQRELS RLSRELEP 918

    Note: No experimental confirmation available.

    Length:918
    Mass (Da):103,533
    Last modified:July 1, 2008 - v2
    Checksum:iDB774BBF7CB4428E
    GO
    Isoform 2 (identifier: Q8VCC8-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         688-689: QE → ELIHYVLGPQ

    Note: No experimental confirmation available.

    Show »
    Length:926
    Mass (Da):104,426
    Checksum:iA8140C76937E5A95
    GO

    Sequence cautioni

    The sequence AAH20532.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei688 – 6892QE → ELIHYVLGPQ in isoform 2. 2 PublicationsVSP_007610

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC020532 mRNA. Translation: AAH20532.1. Different initiation.
    BC020311 mRNA. Translation: AAH20311.1.
    AK034265 mRNA. Translation: BAC28653.1.
    CCDSiCCDS37187.2. [Q8VCC8-1]
    CCDS49714.1. [Q8VCC8-2]
    RefSeqiNP_001171281.1. NM_001177810.1. [Q8VCC8-2]
    NP_001171282.1. NM_001177811.1.
    NP_659099.2. NM_144850.2. [Q8VCC8-1]
    XP_006520940.1. XM_006520877.1. [Q8VCC8-2]
    UniGeneiMm.24028.

    Genome annotation databases

    EnsembliENSMUST00000126854; ENSMUSP00000116426; ENSMUSG00000022469. [Q8VCC8-2]
    ENSMUST00000129223; ENSMUSP00000118148; ENSMUSG00000022469. [Q8VCC8-1]
    GeneIDi223864.
    KEGGimmu:223864.
    UCSCiuc007xkz.2. mouse. [Q8VCC8-2]
    uc007xla.2. mouse. [Q8VCC8-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC020532 mRNA. Translation: AAH20532.1 . Different initiation.
    BC020311 mRNA. Translation: AAH20311.1 .
    AK034265 mRNA. Translation: BAC28653.1 .
    CCDSi CCDS37187.2. [Q8VCC8-1 ]
    CCDS49714.1. [Q8VCC8-2 ]
    RefSeqi NP_001171281.1. NM_001177810.1. [Q8VCC8-2 ]
    NP_001171282.1. NM_001177811.1.
    NP_659099.2. NM_144850.2. [Q8VCC8-1 ]
    XP_006520940.1. XM_006520877.1. [Q8VCC8-2 ]
    UniGenei Mm.24028.

    3D structure databases

    ProteinModelPortali Q8VCC8.
    SMRi Q8VCC8. Positions 89-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 230205. 1 interaction.

    PTM databases

    PhosphoSitei Q8VCC8.

    Proteomic databases

    PaxDbi Q8VCC8.
    PRIDEi Q8VCC8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000126854 ; ENSMUSP00000116426 ; ENSMUSG00000022469 . [Q8VCC8-2 ]
    ENSMUST00000129223 ; ENSMUSP00000118148 ; ENSMUSG00000022469 . [Q8VCC8-1 ]
    GeneIDi 223864.
    KEGGi mmu:223864.
    UCSCi uc007xkz.2. mouse. [Q8VCC8-2 ]
    uc007xla.2. mouse. [Q8VCC8-1 ]

    Organism-specific databases

    CTDi 10411.
    MGIi MGI:2441741. Rapgef3.

    Phylogenomic databases

    eggNOGi NOG236726.
    GeneTreei ENSGT00750000117452.
    HOGENOMi HOG000230545.
    HOVERGENi HBG056985.
    InParanoidi Q8VCC8.
    KOi K08014.
    OMAi NSFFAVM.
    OrthoDBi EOG7ZD1TF.
    PhylomeDBi Q8VCC8.
    TreeFami TF313184.

    Miscellaneous databases

    ChiTaRSi RAPGEF3. mouse.
    NextBioi 376937.
    PROi Q8VCC8.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q8VCC8.
    Bgeei Q8VCC8.
    CleanExi MM_RAPGEF3.
    Genevestigatori Q8VCC8.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.840.10. 1 hit.
    2.60.120.10. 1 hit.
    InterProi IPR002373. cAMP/cGMP_kin.
    IPR018490. cNMP-bd-like.
    IPR000595. cNMP-bd_dom.
    IPR000591. DEP_dom.
    IPR000651. Ras-like_Gua-exchang_fac_N.
    IPR019804. Ras_G-nucl-exch_fac_CS.
    IPR023578. Ras_GEF_dom.
    IPR001895. RasGRF_CDC25.
    IPR014710. RmlC-like_jellyroll.
    IPR029071. Ubiquitin-rel_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 1 hit.
    PF00610. DEP. 1 hit.
    PF00617. RasGEF. 1 hit.
    PF00618. RasGEF_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00103. CAMPKINASE.
    SMARTi SM00100. cNMP. 1 hit.
    SM00049. DEP. 1 hit.
    SM00147. RasGEF. 1 hit.
    SM00229. RasGEFN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48366. SSF48366. 3 hits.
    SSF51206. SSF51206. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
    PS50186. DEP. 1 hit.
    PS00720. RASGEF. 1 hit.
    PS50009. RASGEF_CAT. 1 hit.
    PS50212. RASGEF_NTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 640-918 (ISOFORM 2).
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 466-918 (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Diencephalon.

    Entry informationi

    Entry nameiRPGF3_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCC8
    Secondary accession number(s): Q8BZK9, Q8R1R1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 106 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3