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Q8VCB3

- GYS2_MOUSE

UniProt

Q8VCB3 - GYS2_MOUSE

Protein

Glycogen [starch] synthase, liver

Gene

Gys2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Transfers the glycosyl residue from UDP-Glc to the non-reducing end of alpha-1,4-glucan.

    Catalytic activityi

    UDP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = UDP + ((1->4)-alpha-D-glucosyl)(n+1).

    Enzyme regulationi

    Allosteric activation by glucose-6-phosphate. Phosphorylation reduces the activity towards UDP-glucose. When in the non-phosphorylated state, glycogen synthase does not require glucose-6-phosphate as an allosteric activator; when phosphorylated it does By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei40 – 401UDP-glucoseBy similarity

    GO - Molecular functioni

    1. glycogen (starch) synthase activity Source: UniProtKB

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB
    2. response to glucose Source: UniProtKB

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Glycogen biosynthesis

    Enzyme and pathway databases

    ReactomeiREACT_188939. Glycogen synthesis.
    REACT_203841. Myoclonic epilepsy of Lafora.
    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiGT3. Glycosyltransferase Family 3.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycogen [starch] synthase, liver (EC:2.4.1.11)
    Gene namesi
    Name:Gys2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:2385254. Gys2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB
    2. cortical actin cytoskeleton Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. cytoskeleton Source: UniProtKB
    5. cytosol Source: UniProtKB
    6. ectoplasm Source: UniProtKB

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 704704Glycogen [starch] synthase, liverPRO_0000274489Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei641 – 6411Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
    Modified residuei645 – 6451Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
    Modified residuei649 – 6491Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
    Modified residuei653 – 6531Phosphoserine; by GSK3-alpha and GSK3-betaBy similarity
    Modified residuei657 – 6571Phosphoserine; by CK2By similarity
    Modified residuei684 – 6841Phosphoserine1 Publication

    Post-translational modificationi

    Primed phosphorylation at Ser-657 (site 5) by CSNK2A1 and CSNK2A2 is required for inhibitory phosphorylation at Ser-641 (site 3a), Ser-645 (site 3b), Ser-649 (site 3c) and Ser-653 (site 4) by GSK3A an GSK3B. Dephosphorylation at Ser-641 and Ser-645 by PP1 activates the enzyme By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ8VCB3.
    PaxDbiQ8VCB3.
    PRIDEiQ8VCB3.

    PTM databases

    PhosphoSiteiQ8VCB3.

    Expressioni

    Inductioni

    Expression in the liver oscillates in a circadian manner with peak levels during the night.1 Publication

    Gene expression databases

    BgeeiQ8VCB3.
    CleanExiMM_GYS2.
    GenevestigatoriQ8VCB3.

    Interactioni

    Protein-protein interaction databases

    IntActiQ8VCB3. 2 interactions.
    MINTiMINT-4119490.
    STRINGi10090.ENSMUSP00000032371.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VCB3.
    SMRiQ8VCB3. Positions 25-621.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 3 family.Curated

    Phylogenomic databases

    eggNOGiCOG0438.
    GeneTreeiENSGT00390000018612.
    HOGENOMiHOG000160890.
    HOVERGENiHBG001960.
    InParanoidiQ3UTY0.
    KOiK00693.
    OMAiEVSWEVT.
    OrthoDBiEOG741Z1N.
    TreeFamiTF300306.

    Family and domain databases

    InterProiIPR008631. Glycogen_synth.
    [Graphical view]
    PANTHERiPTHR10176. PTHR10176. 1 hit.
    PfamiPF05693. Glycogen_syn. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VCB3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRGRSLSVT SLGGLPVWEA ERLPVEDLLL FEVSWEVTNK VGGICTVIQT    50
    KAKTTADEWG ENYFLIGPYF EHNMKTQVEQ CEPTNDAVRK AVDAMNKHGC 100
    QVHFGRWLIE GSPYVVLFDI SSSAWNLDRW KGDFWEACGV GIPHHDREAN 150
    DMLIFGSLTA WFLKEVTDHA DGKHVIAQFH EWQAGTGLIL SRARKLPIAT 200
    VFTTHATLLG RYLCAANIDF YNQLDKFDID KEAGERQIYH RYCMERASVH 250
    CAHVFTTVSE ITAIEAEHML KRKPDVVTPN GLNVKKFSAV HEFQNLHAMY 300
    KARIQDFVRG HFYGHLDFDL EKTLFLFIAG RYEFSNKGAD IFLESLSRLN 350
    FLLRMHKSNV TVVVFFIMPA KTNNFNVETL KGQAVRKQLW DTVHCLKEKF 400
    GKKLYDGLLR GEIPDMNSIL DRDDLTIMKR AIFSTQRQSL PPVTTHNMID 450
    DSTDPILSTI RRIGLFNNRA DRVKVILHPE FLSSTSPLLP MDYEEFVRGC 500
    HLGVFPSYYE PWGYTPAECT VMGIPSVTTN LSGFGCFVQE HVADPTAYGI 550
    YIVDRRFRSP DDSCNQLTQF LYGFCKQSRR QRIIQRNRTE RLSDLLDWRY 600
    LGRYYQHARH LTLSRAFPDK FHLEPTSPPT TDGFKYPRPS SVPPSPSGSQ 650
    ASSPQCSDAE DEEDEDERYD EEEEAERDRL NIKSPFSLNH FPKGKKKLHG 700
    EYKN 704
    Length:704
    Mass (Da):80,871
    Last modified:July 27, 2011 - v2
    Checksum:iC4B87195B5FC41FE
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti630 – 6301T → M in AAH21322. (PubMed:15489334)Curated
    Sequence conflicti656 – 6561C → S in AAH21322. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK138992 mRNA. Translation: BAE23850.1.
    BC021322 mRNA. Translation: AAH21322.1.
    BC158081 mRNA. Translation: AAI58082.1.
    CCDSiCCDS20683.1.
    RefSeqiNP_663547.2. NM_145572.2.
    UniGeneiMm.275975.

    Genome annotation databases

    EnsembliENSMUST00000032371; ENSMUSP00000032371; ENSMUSG00000030244.
    GeneIDi232493.
    KEGGimmu:232493.
    UCSCiuc009epi.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK138992 mRNA. Translation: BAE23850.1 .
    BC021322 mRNA. Translation: AAH21322.1 .
    BC158081 mRNA. Translation: AAI58082.1 .
    CCDSi CCDS20683.1.
    RefSeqi NP_663547.2. NM_145572.2.
    UniGenei Mm.275975.

    3D structure databases

    ProteinModelPortali Q8VCB3.
    SMRi Q8VCB3. Positions 25-621.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q8VCB3. 2 interactions.
    MINTi MINT-4119490.
    STRINGi 10090.ENSMUSP00000032371.

    Protein family/group databases

    CAZyi GT3. Glycosyltransferase Family 3.

    PTM databases

    PhosphoSitei Q8VCB3.

    Proteomic databases

    MaxQBi Q8VCB3.
    PaxDbi Q8VCB3.
    PRIDEi Q8VCB3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032371 ; ENSMUSP00000032371 ; ENSMUSG00000030244 .
    GeneIDi 232493.
    KEGGi mmu:232493.
    UCSCi uc009epi.2. mouse.

    Organism-specific databases

    CTDi 2998.
    MGIi MGI:2385254. Gys2.

    Phylogenomic databases

    eggNOGi COG0438.
    GeneTreei ENSGT00390000018612.
    HOGENOMi HOG000160890.
    HOVERGENi HBG001960.
    InParanoidi Q3UTY0.
    KOi K00693.
    OMAi EVSWEVT.
    OrthoDBi EOG741Z1N.
    TreeFami TF300306.

    Enzyme and pathway databases

    UniPathwayi UPA00164 .
    Reactomei REACT_188939. Glycogen synthesis.
    REACT_203841. Myoclonic epilepsy of Lafora.

    Miscellaneous databases

    NextBioi 381135.
    PROi Q8VCB3.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VCB3.
    CleanExi MM_GYS2.
    Genevestigatori Q8VCB3.

    Family and domain databases

    InterProi IPR008631. Glycogen_synth.
    [Graphical view ]
    PANTHERi PTHR10176. PTHR10176. 1 hit.
    Pfami PF05693. Glycogen_syn. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Aorta and Vein.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-684, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "CLOCK regulates circadian rhythms of hepatic glycogen synthesis through transcriptional activation of Gys2."
      Doi R., Oishi K., Ishida N.
      J. Biol. Chem. 285:22114-22121(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiGYS2_MOUSE
    AccessioniPrimary (citable) accession number: Q8VCB3
    Secondary accession number(s): Q3UTY0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 6, 2007
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3