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Protein

Mediator of RNA polymerase II transcription subunit 25

Gene

Med25

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for RARA/RXRA-mediated transcription (By similarity).By similarity

GO - Molecular functioni

  • retinoic acid receptor binding Source: UniProtKB
  • retinoid X receptor binding Source: UniProtKB
  • transcription factor binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 25
Alternative name(s):
Mediator complex subunit 25
Short name:
mMED25
Gene namesi
Name:Med25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1922863. Med25.

Subcellular locationi

GO - Cellular componenti

  • nuclear transcription factor complex Source: MGI
  • nucleoplasm Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003049531 – 745Mediator of RNA polymerase II transcription subunit 25Add BLAST745

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei723Asymmetric dimethylarginineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiQ8VCB2.
PeptideAtlasiQ8VCB2.
PRIDEiQ8VCB2.

PTM databases

iPTMnetiQ8VCB2.
PhosphoSitePlusiQ8VCB2.

Expressioni

Gene expression databases

BgeeiENSMUSG00000002968.
ExpressionAtlasiQ8VCB2. baseline and differential.
GenevisibleiQ8VCB2. MM.

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP. Interacts with CREBBP. Interacts with ESR1, GR and THRB in a ligand-dependent fashion. Binds the Herpes simplex virus activator VP16 (By similarity). Interacts with RARA and RXRA in a ligand-dependent fashion.By similarity1 Publication

GO - Molecular functioni

  • retinoic acid receptor binding Source: UniProtKB
  • retinoid X receptor binding Source: UniProtKB
  • transcription factor binding Source: MGI

Protein-protein interaction databases

BioGridi217616. 1 interactor.
IntActiQ8VCB2. 5 interactors.
MINTiMINT-5210237.
STRINGi10090.ENSMUSP00000003049.

Structurei

3D structure databases

ProteinModelPortaliQ8VCB2.
SMRiQ8VCB2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 226Interaction with the Mediator complexBy similarityAdd BLAST226
Regioni389 – 543Interaction with VP16By similarityAdd BLAST155
Regioni395 – 545Interaction with CREBBPBy similarityAdd BLAST151
Regioni563 – 652Interaction with RARABy similarityAdd BLAST90
Regioni639 – 705Interaction with RARABy similarityAdd BLAST67

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi645 – 649LXXLL motif5

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi246 – 365Pro-richAdd BLAST120
Compositional biasi546 – 611Gln-richAdd BLAST66

Sequence similaritiesi

Belongs to the Mediator complex subunit 25 family.Curated

Phylogenomic databases

eggNOGiENOG410IHD9. Eukaryota.
ENOG410Z0HI. LUCA.
GeneTreeiENSGT00520000055653.
HOGENOMiHOG000113538.
HOVERGENiHBG108127.
InParanoidiQ8VCB2.
KOiK15168.
OrthoDBiEOG091G04LS.
PhylomeDBiQ8VCB2.
TreeFamiTF329598.

Family and domain databases

InterProiIPR021394. Mediator_Med25.
IPR021406. Mediator_Med25_NR-box.
IPR021397. Mediator_Med25_SD1.
IPR021419. Mediator_Med25_VWA.
IPR002035. VWF_A.
[Graphical view]
PfamiPF11232. Med25. 1 hit.
PF11244. Med25_NR-box. 1 hit.
PF11235. Med25_SD1. 1 hit.
PF11265. Med25_VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q8VCB2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVPGSEGPAR AGGLVADVVF VIEGTANLGP YFEELRKHYL LPAIEYFNGG
60 70 80 90 100
PPAETDFGGD YGGTQYSLVV FNTVDCAPES YVQCHAPTSS AYEFVTWLDG
110 120 130 140 150
IKFMGGGGES CSLIAEGLST ALQLFDDFKK MREQIGQTHR VCLLICNSPP
160 170 180 190 200
YLLPAVESTT YSGCTTESLV QKIGERGIHF SIVSPRKLPA LRLLFEKAAP
210 220 230 240 250
PALLEPLQQP ADVSQDPRHM VLVRGLVLPV GGSSTSGSLQ TKQAVPLPPA
260 270 280 290 300
PASAATLSAA PPQALPPVPP QYQVPGNLSA AQVAAQNAVE AAKSQKAGLG
310 320 330 340 350
PRFSPINPLQ QAAPGVGPPF SQAPAPPLAP VPPGAPKPPP ASQPSLVSTV
360 370 380 390 400
APGPVLAAPA QPGAPSLAGT VTPGGVNGPS AAQLGGPALG GQQSVSNKLL
410 420 430 440 450
AWSGVLEWQE KPKPASVDAN TKLTRSLPCQ VYVNHGENLK TEQWPQKLIM
460 470 480 490 500
QLIPQQLLTT LGPLFRNSRM VQFHFTNKDL ESLKGLYRIM GNGFAGCVHF
510 520 530 540 550
PHTAPCEVRV LMLLYSSKKK IFMGLIPYDQ SGFVNGIRQV ITNHKQVQQQ
560 570 580 590 600
KLEQQRGMGA QQAPPVLGPI LEEQARPPQN LLQLRAPQPQ PQGAVGASAA
610 620 630 640 650
TGQPQPQGAT QAPTGAPQGP PGAAPGPPPS GPILRPQNPG ANPQLRSLLL
660 670 680 690 700
NPAPPQTGVP PPQASLHHLQ PPGAPTLLPP HQSMGQPQLG PQLLHPPPAQ
710 720 730 740
SWPTQLPQRA PLPGQMLLSG GPRGPVPQPG LQPSVMEDDI LMDLI
Length:745
Mass (Da):78,113
Last modified:March 1, 2002 - v1
Checksum:iA8C63CF063BECEEA
GO
Isoform 2 (identifier: Q8VCB2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-103: Missing.
     558-581: Missing.

Show »
Length:618
Mass (Da):64,506
Checksum:iFF8E7568526AC470
GO
Isoform 3 (identifier: Q8VCB2-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-102: K → KPVLGAEPYP...YEDSTTFGQM

Note: No experimental confirmation available.
Show »
Length:822
Mass (Da):86,585
Checksum:i76EE6D0B4A35AF61
GO
Isoform 4 (identifier: Q8VCB2-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     103-745: Missing.

Show »
Length:102
Mass (Da):10,940
Checksum:i5AF83A18D99C2CEC
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0281471 – 103Missing in isoform 2. 1 PublicationAdd BLAST103
Alternative sequenceiVSP_028148102K → KPVLGAEPYPDPARMSNSLS LLPANGIPRKLSSGIVKCRC LLRTSGRNPHLEFWEAPRPS LLHIRDGAYEDSTTFGQM in isoform 3. 1 Publication1
Alternative sequenceiVSP_028149103 – 745Missing in isoform 4. 1 PublicationAdd BLAST643
Alternative sequenceiVSP_028150558 – 581Missing in isoform 2. 1 PublicationAdd BLAST24

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK148368 mRNA. Translation: BAE28509.1.
AK149867 mRNA. Translation: BAE29135.1.
BC021333 mRNA. Translation: AAH21333.1.
BC031138 mRNA. Translation: AAH31138.1.
BC071206 mRNA. Translation: AAH71206.1.
CCDSiCCDS21220.1. [Q8VCB2-1]
RefSeqiNP_083641.1. NM_029365.3. [Q8VCB2-1]
UniGeneiMm.157778.

Genome annotation databases

EnsembliENSMUST00000003049; ENSMUSP00000003049; ENSMUSG00000002968. [Q8VCB2-1]
ENSMUST00000207278; ENSMUSP00000146595; ENSMUSG00000002968. [Q8VCB2-2]
GeneIDi75613.
KEGGimmu:75613.
UCSCiuc009grm.1. mouse. [Q8VCB2-2]
uc009grn.1. mouse. [Q8VCB2-1]
uc009grs.1. mouse. [Q8VCB2-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK148368 mRNA. Translation: BAE28509.1.
AK149867 mRNA. Translation: BAE29135.1.
BC021333 mRNA. Translation: AAH21333.1.
BC031138 mRNA. Translation: AAH31138.1.
BC071206 mRNA. Translation: AAH71206.1.
CCDSiCCDS21220.1. [Q8VCB2-1]
RefSeqiNP_083641.1. NM_029365.3. [Q8VCB2-1]
UniGeneiMm.157778.

3D structure databases

ProteinModelPortaliQ8VCB2.
SMRiQ8VCB2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217616. 1 interactor.
IntActiQ8VCB2. 5 interactors.
MINTiMINT-5210237.
STRINGi10090.ENSMUSP00000003049.

PTM databases

iPTMnetiQ8VCB2.
PhosphoSitePlusiQ8VCB2.

Proteomic databases

PaxDbiQ8VCB2.
PeptideAtlasiQ8VCB2.
PRIDEiQ8VCB2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000003049; ENSMUSP00000003049; ENSMUSG00000002968. [Q8VCB2-1]
ENSMUST00000207278; ENSMUSP00000146595; ENSMUSG00000002968. [Q8VCB2-2]
GeneIDi75613.
KEGGimmu:75613.
UCSCiuc009grm.1. mouse. [Q8VCB2-2]
uc009grn.1. mouse. [Q8VCB2-1]
uc009grs.1. mouse. [Q8VCB2-3]

Organism-specific databases

CTDi81857.
MGIiMGI:1922863. Med25.

Phylogenomic databases

eggNOGiENOG410IHD9. Eukaryota.
ENOG410Z0HI. LUCA.
GeneTreeiENSGT00520000055653.
HOGENOMiHOG000113538.
HOVERGENiHBG108127.
InParanoidiQ8VCB2.
KOiK15168.
OrthoDBiEOG091G04LS.
PhylomeDBiQ8VCB2.
TreeFamiTF329598.

Enzyme and pathway databases

ReactomeiR-MMU-212436. Generic Transcription Pathway.
R-MMU-381340. Transcriptional regulation of white adipocyte differentiation.
R-MMU-442533. Transcriptional Regulation of Adipocyte Differentiation in 3T3-L1 Pre-adipocytes.

Miscellaneous databases

ChiTaRSiMed25. mouse.
PROiQ8VCB2.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000002968.
ExpressionAtlasiQ8VCB2. baseline and differential.
GenevisibleiQ8VCB2. MM.

Family and domain databases

InterProiIPR021394. Mediator_Med25.
IPR021406. Mediator_Med25_NR-box.
IPR021397. Mediator_Med25_SD1.
IPR021419. Mediator_Med25_VWA.
IPR002035. VWF_A.
[Graphical view]
PfamiPF11232. Med25. 1 hit.
PF11244. Med25_NR-box. 1 hit.
PF11235. Med25_SD1. 1 hit.
PF11265. Med25_VWA. 1 hit.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMED25_MOUSE
AccessioniPrimary (citable) accession number: Q8VCB2
Secondary accession number(s): Q3UDX6, Q3UFQ1, Q6IR27
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.