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Q8VC88

- GRAN_MOUSE

UniProt

Q8VC88 - GRAN_MOUSE

Protein

Grancalcin

Gene

Gca

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi105 – 116121PROSITE-ProRule annotationAdd
    BLAST
    Calcium bindingi135 – 146122PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. calcium-dependent cysteine-type endopeptidase activity Source: RefGenome
    2. calcium ion binding Source: UniProtKB

    GO - Biological processi

    1. proteolysis Source: RefGenome

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Grancalcin
    Gene namesi
    Name:Gca
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:1918521. Gca.

    Subcellular locationi

    Cytoplasm By similarity. Cytoplasmic granule membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Primarily cytosolic in the absence of calcium or magnesium ions. Relocates to granules and other membranes in response to elevated calcium and magnesium levels By similarity.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. cytosol Source: MGI
    3. plasma membrane Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Not essential for normal resistance to microbial infections. Grancalcin-deficient neutrophils exhibit decreased adhesion to fibronectin, and a strongly reduced number of focal adhesion complexes.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 220220GrancalcinPRO_0000073722Add
    BLAST

    Proteomic databases

    MaxQBiQ8VC88.
    PaxDbiQ8VC88.
    PRIDEiQ8VC88.

    2D gel databases

    REPRODUCTION-2DPAGEIPI00308789.

    PTM databases

    PhosphoSiteiQ8VC88.

    Expressioni

    Gene expression databases

    BgeeiQ8VC88.
    CleanExiMM_GCA.
    GenevestigatoriQ8VC88.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with SRI and LCP1 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VC88.
    SMRiQ8VC88. Positions 56-220.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini51 – 8636EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini92 – 12736EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini122 – 15736EF-hand 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini158 – 19336EF-hand 4PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 4 EF-hand domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG298587.
    GeneTreeiENSGT00620000087734.
    HOGENOMiHOG000231982.
    HOVERGENiHBG004492.
    InParanoidiQ8VC88.
    OMAiSAGDPMW.
    OrthoDBiEOG7RV9FM.
    PhylomeDBiQ8VC88.
    TreeFamiTF314682.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view]
    SMARTiSM00054. EFh. 2 hits.
    [Graphical view]
    PROSITEiPS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VC88-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYPGYGGAF GNFSGQIPGM QMQMGQPMPG AGPNMFSGGY PGYLGYSDSY    50
    SPADDSMWTY FTAVAGQDGE VDAEELQRCL TQSGISGTYA PFSLETCRIM 100
    IAMLDRDYTG KMGFNEFKEL WAALNAWKQN FMTIDQDQSG TVEHHELSQA 150
    IALMGYRLSP QTLAAIVRRY SKNGRIFFDD YVACCVKLRA LTDFFRRRDH 200
    LQQGIVNFMY EDFLQGTMTI 220
    Length:220
    Mass (Da):24,663
    Last modified:March 1, 2002 - v1
    Checksum:i694DCA7D6ADF8279
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti187 – 1882KL → NV in AAM66720. (PubMed:12529388)Curated
    Sequence conflicti217 – 2171T → P in BAC32686. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF518325 mRNA. Translation: AAM66720.1.
    AB088388 mRNA. Translation: BAC07231.1.
    AK033740 mRNA. Translation: BAC28458.1.
    AK046347 mRNA. Translation: BAC32686.1.
    AK049317 mRNA. Translation: BAC33679.1.
    AK050540 mRNA. Translation: BAC34315.1.
    AK078915 mRNA. Translation: BAC37457.1.
    BC021450 mRNA. Translation: AAH21450.1.
    CCDSiCCDS16069.1.
    RefSeqiNP_663498.1. NM_145523.3.
    UniGeneiMm.219877.

    Genome annotation databases

    EnsembliENSMUST00000028257; ENSMUSP00000028257; ENSMUSG00000026893.
    GeneIDi227960.
    KEGGimmu:227960.
    UCSCiuc008jvp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF518325 mRNA. Translation: AAM66720.1 .
    AB088388 mRNA. Translation: BAC07231.1 .
    AK033740 mRNA. Translation: BAC28458.1 .
    AK046347 mRNA. Translation: BAC32686.1 .
    AK049317 mRNA. Translation: BAC33679.1 .
    AK050540 mRNA. Translation: BAC34315.1 .
    AK078915 mRNA. Translation: BAC37457.1 .
    BC021450 mRNA. Translation: AAH21450.1 .
    CCDSi CCDS16069.1.
    RefSeqi NP_663498.1. NM_145523.3.
    UniGenei Mm.219877.

    3D structure databases

    ProteinModelPortali Q8VC88.
    SMRi Q8VC88. Positions 56-220.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q8VC88.

    2D gel databases

    REPRODUCTION-2DPAGE IPI00308789.

    Proteomic databases

    MaxQBi Q8VC88.
    PaxDbi Q8VC88.
    PRIDEi Q8VC88.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028257 ; ENSMUSP00000028257 ; ENSMUSG00000026893 .
    GeneIDi 227960.
    KEGGi mmu:227960.
    UCSCi uc008jvp.1. mouse.

    Organism-specific databases

    CTDi 25801.
    MGIi MGI:1918521. Gca.

    Phylogenomic databases

    eggNOGi NOG298587.
    GeneTreei ENSGT00620000087734.
    HOGENOMi HOG000231982.
    HOVERGENi HBG004492.
    InParanoidi Q8VC88.
    OMAi SAGDPMW.
    OrthoDBi EOG7RV9FM.
    PhylomeDBi Q8VC88.
    TreeFami TF314682.

    Miscellaneous databases

    NextBioi 378876.
    PROi Q8VC88.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VC88.
    CleanExi MM_GCA.
    Genevestigatori Q8VC88.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    [Graphical view ]
    SMARTi SM00054. EFh. 2 hits.
    [Graphical view ]
    PROSITEi PS00018. EF_HAND_1. 1 hit.
    PS50222. EF_HAND_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Granulocyte function in grancalcin-deficient mice."
      Roes J., Choi B.K., Power D., Xu P., Segal A.W.
      Mol. Cell. Biol. 23:826-830(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
    2. "Characterization of murine grancalcin specifically expressed in leukocytes and its possible role in host defense against bacterial infection."
      Liu F., Shinomiya H., Kirikae T., Hirata H., Asano Y.
      Biosci. Biotechnol. Biochem. 68:894-902(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cecum, Corpora quadrigemina and Pancreas.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Kidney.
    5. "The role of grancalcin in adhesion of neutrophils."
      Xu P., Roes J., Segal A.W., Radulovic M.
      Cell. Immunol. 240:116-121(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry nameiGRAN_MOUSE
    AccessioniPrimary (citable) accession number: Q8VC88
    Secondary accession number(s): Q8BL53, Q8K3Z6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 102 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This protein has been shown to bind calcium with high affinity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3