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Protein

Grancalcin

Gene

Gca

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi105 – 116121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi135 – 146122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Grancalcin
Gene namesi
Name:Gca
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1918521. Gca.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytosol Source: MGI
  • extracellular exosome Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Not essential for normal resistance to microbial infections. Grancalcin-deficient neutrophils exhibit decreased adhesion to fibronectin, and a strongly reduced number of focal adhesion complexes.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 220220GrancalcinPRO_0000073722Add
BLAST

Proteomic databases

MaxQBiQ8VC88.
PaxDbiQ8VC88.
PRIDEiQ8VC88.

2D gel databases

REPRODUCTION-2DPAGEIPI00308789.

PTM databases

PhosphoSiteiQ8VC88.

Expressioni

Gene expression databases

BgeeiQ8VC88.
CleanExiMM_GCA.
GenevestigatoriQ8VC88.

Interactioni

Subunit structurei

Homodimer. Interacts with SRI and LCP1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ8VC88.
SMRiQ8VC88. Positions 56-220.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini51 – 8636EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini92 – 12736EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini122 – 15736EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini158 – 19336EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG298587.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000231982.
HOVERGENiHBG004492.
InParanoidiQ8VC88.
OMAiFMTIDQD.
OrthoDBiEOG7RV9FM.
PhylomeDBiQ8VC88.
TreeFamiTF314682.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8VC88-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYPGYGGAF GNFSGQIPGM QMQMGQPMPG AGPNMFSGGY PGYLGYSDSY
60 70 80 90 100
SPADDSMWTY FTAVAGQDGE VDAEELQRCL TQSGISGTYA PFSLETCRIM
110 120 130 140 150
IAMLDRDYTG KMGFNEFKEL WAALNAWKQN FMTIDQDQSG TVEHHELSQA
160 170 180 190 200
IALMGYRLSP QTLAAIVRRY SKNGRIFFDD YVACCVKLRA LTDFFRRRDH
210 220
LQQGIVNFMY EDFLQGTMTI
Length:220
Mass (Da):24,663
Last modified:March 1, 2002 - v1
Checksum:i694DCA7D6ADF8279
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti187 – 1882KL → NV in AAM66720 (PubMed:12529388).Curated
Sequence conflicti217 – 2171T → P in BAC32686 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF518325 mRNA. Translation: AAM66720.1.
AB088388 mRNA. Translation: BAC07231.1.
AK033740 mRNA. Translation: BAC28458.1.
AK046347 mRNA. Translation: BAC32686.1.
AK049317 mRNA. Translation: BAC33679.1.
AK050540 mRNA. Translation: BAC34315.1.
AK078915 mRNA. Translation: BAC37457.1.
BC021450 mRNA. Translation: AAH21450.1.
CCDSiCCDS16069.1.
RefSeqiNP_663498.1. NM_145523.3.
UniGeneiMm.219877.

Genome annotation databases

EnsembliENSMUST00000028257; ENSMUSP00000028257; ENSMUSG00000026893.
GeneIDi227960.
KEGGimmu:227960.
UCSCiuc008jvp.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF518325 mRNA. Translation: AAM66720.1.
AB088388 mRNA. Translation: BAC07231.1.
AK033740 mRNA. Translation: BAC28458.1.
AK046347 mRNA. Translation: BAC32686.1.
AK049317 mRNA. Translation: BAC33679.1.
AK050540 mRNA. Translation: BAC34315.1.
AK078915 mRNA. Translation: BAC37457.1.
BC021450 mRNA. Translation: AAH21450.1.
CCDSiCCDS16069.1.
RefSeqiNP_663498.1. NM_145523.3.
UniGeneiMm.219877.

3D structure databases

ProteinModelPortaliQ8VC88.
SMRiQ8VC88. Positions 56-220.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ8VC88.

2D gel databases

REPRODUCTION-2DPAGEIPI00308789.

Proteomic databases

MaxQBiQ8VC88.
PaxDbiQ8VC88.
PRIDEiQ8VC88.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028257; ENSMUSP00000028257; ENSMUSG00000026893.
GeneIDi227960.
KEGGimmu:227960.
UCSCiuc008jvp.1. mouse.

Organism-specific databases

CTDi25801.
MGIiMGI:1918521. Gca.

Phylogenomic databases

eggNOGiNOG298587.
GeneTreeiENSGT00620000087734.
HOGENOMiHOG000231982.
HOVERGENiHBG004492.
InParanoidiQ8VC88.
OMAiFMTIDQD.
OrthoDBiEOG7RV9FM.
PhylomeDBiQ8VC88.
TreeFamiTF314682.

Miscellaneous databases

NextBioi378876.
PROiQ8VC88.
SOURCEiSearch...

Gene expression databases

BgeeiQ8VC88.
CleanExiMM_GCA.
GenevestigatoriQ8VC88.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SMARTiSM00054. EFh. 2 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Granulocyte function in grancalcin-deficient mice."
    Roes J., Choi B.K., Power D., Xu P., Segal A.W.
    Mol. Cell. Biol. 23:826-830(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
  2. "Characterization of murine grancalcin specifically expressed in leukocytes and its possible role in host defense against bacterial infection."
    Liu F., Shinomiya H., Kirikae T., Hirata H., Asano Y.
    Biosci. Biotechnol. Biochem. 68:894-902(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum, Corpora quadrigemina and Pancreas.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Kidney.
  5. "The role of grancalcin in adhesion of neutrophils."
    Xu P., Roes J., Segal A.W., Radulovic M.
    Cell. Immunol. 240:116-121(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiGRAN_MOUSE
AccessioniPrimary (citable) accession number: Q8VC88
Secondary accession number(s): Q8BL53, Q8K3Z6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2002
Last modified: May 27, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

This protein has been shown to bind calcium with high affinity.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.