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Q8VC88 (GRAN_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Grancalcin
Gene names
Name:Gca
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Calcium-binding protein that may play a role in the adhesion of neutrophils to fibronectin. May play a role in the formation of focal adhesions. Ref.5

Subunit structure

Homodimer. Interacts with SRI and LCP1 By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmic granule membrane; Peripheral membrane protein; Cytoplasmic side By similarity. Note: Primarily cytosolic in the absence of calcium or magnesium ions. Relocates to granules and other membranes in response to elevated calcium and magnesium levels By similarity.

Disruption phenotype

No visible phenotype. Not essential for normal resistance to microbial infections. Grancalcin-deficient neutrophils exhibit decreased adhesion to fibronectin, and a strongly reduced number of focal adhesion complexes. Ref.1 Ref.5

Miscellaneous

This protein has been shown to bind calcium with high affinity By similarity.

Sequence similarities

Contains 4 EF-hand domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Grancalcin
PRO_0000073722

Regions

Domain51 – 8636EF-hand 1
Domain92 – 12736EF-hand 2
Domain122 – 15736EF-hand 3
Domain158 – 19336EF-hand 4
Calcium binding105 – 116121 Potential
Calcium binding135 – 146122 Potential

Experimental info

Sequence conflict187 – 1882KL → NV in AAM66720. Ref.1
Sequence conflict2171T → P in BAC32686. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Q8VC88 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: 694DCA7D6ADF8279

FASTA22024,663
        10         20         30         40         50         60 
MAYPGYGGAF GNFSGQIPGM QMQMGQPMPG AGPNMFSGGY PGYLGYSDSY SPADDSMWTY 

        70         80         90        100        110        120 
FTAVAGQDGE VDAEELQRCL TQSGISGTYA PFSLETCRIM IAMLDRDYTG KMGFNEFKEL 

       130        140        150        160        170        180 
WAALNAWKQN FMTIDQDQSG TVEHHELSQA IALMGYRLSP QTLAAIVRRY SKNGRIFFDD 

       190        200        210        220 
YVACCVKLRA LTDFFRRRDH LQQGIVNFMY EDFLQGTMTI 

« Hide

References

« Hide 'large scale' references
[1]"Granulocyte function in grancalcin-deficient mice."
Roes J., Choi B.K., Power D., Xu P., Segal A.W.
Mol. Cell. Biol. 23:826-830(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], DISRUPTION PHENOTYPE.
[2]"Characterization of murine grancalcin specifically expressed in leukocytes and its possible role in host defense against bacterial infection."
Liu F., Shinomiya H., Kirikae T., Hirata H., Asano Y.
Biosci. Biotechnol. Biochem. 68:894-902(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Cecum, Corpora quadrigemina and Pancreas.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Kidney.
[5]"The role of grancalcin in adhesion of neutrophils."
Xu P., Roes J., Segal A.W., Radulovic M.
Cell. Immunol. 240:116-121(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF518325 mRNA. Translation: AAM66720.1.
AB088388 mRNA. Translation: BAC07231.1.
AK033740 mRNA. Translation: BAC28458.1.
AK046347 mRNA. Translation: BAC32686.1.
AK049317 mRNA. Translation: BAC33679.1.
AK050540 mRNA. Translation: BAC34315.1.
AK078915 mRNA. Translation: BAC37457.1.
BC021450 mRNA. Translation: AAH21450.1.
CCDSCCDS16069.1.
RefSeqNP_663498.1. NM_145523.3.
UniGeneMm.219877.

3D structure databases

ProteinModelPortalQ8VC88.
SMRQ8VC88. Positions 56-220.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ8VC88.

2D gel databases

REPRODUCTION-2DPAGEIPI00308789.

Proteomic databases

MaxQBQ8VC88.
PaxDbQ8VC88.
PRIDEQ8VC88.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028257; ENSMUSP00000028257; ENSMUSG00000026893.
GeneID227960.
KEGGmmu:227960.
UCSCuc008jvp.1. mouse.

Organism-specific databases

CTD25801.
MGIMGI:1918521. Gca.

Phylogenomic databases

eggNOGNOG298587.
GeneTreeENSGT00620000087734.
HOGENOMHOG000231982.
HOVERGENHBG004492.
InParanoidQ8VC88.
OMASAGDPMW.
OrthoDBEOG7RV9FM.
PhylomeDBQ8VC88.
TreeFamTF314682.

Gene expression databases

BgeeQ8VC88.
CleanExMM_GCA.
GenevestigatorQ8VC88.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
SMARTSM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio378876.
PROQ8VC88.
SOURCESearch...

Entry information

Entry nameGRAN_MOUSE
AccessionPrimary (citable) accession number: Q8VC88
Secondary accession number(s): Q8BL53, Q8K3Z6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2002
Last modified: July 9, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot