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Q8VC69

- S22A6_MOUSE

UniProt

Q8VC69 - S22A6_MOUSE

Protein

Solute carrier family 22 member 6

Gene

Slc22a6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Mar 2002)
      Previous versions | rss
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    Functioni

    Involved in the renal elimination of endogenous and exogenous organic anions. Functions as organic anion exchanger when the uptake of one molecule of organic anion is coupled with an efflux of one molecule of endogenous dicarboxylic acid (glutarate, ketoglutarate, etc). Mediates the sodium-independent uptake of 2,3-dimercapto-1-propanesulfonic acid (DMPS), cidofovir, adefovir, 9-(2-phosphonylmethoxyethyl) guanine (PMEG), 9-(2-phosphonylmethoxyethyl) diaminopurine (PMEDAP), ochratoxin (OTA), acyclovir (ACV), 3'-azido-3-'deoxythymidine (AZT), cimetidine (CMD), 2,4-dichloro-phenoxyacetate (2,4-D), hippurate (HA), indoleacetate (IA), indoxyl sulfate (IS) and 3-carboxy-4-methyl-5-propyl-2-furanpropionate (CMPF) and edaravone sulfate By similarity. Mediates the sodium-independent uptake of p-aminohippurate (PAH). PAH uptake is inhibited by benzothiazolylcysteine (BTC), S-chlorotrifluoroethylcysteine (CTFC), cysteine S-conjugates S-dichlorovinylcysteine (DCVC), furosemide, steviol, phorbol 12-myristate 13-acetate (PMA), calcium ionophore A23187, benzylpenicillin, bumetamide, losartan, probenecid, phenol red, urate, glutarate and alpha-ketoglutarate By similarity. PAH uptake is inhibited by p-chloromercuribenzenesulphonate (PCMBS), diethyl pyrocarbonate (DEPC), indomethacin, sulindac, diclofenac, carprofen, okadaic acid and PKC activators.By similarity3 Publications

    Kineticsi

    1. KM=37.3 µM for PAH1 Publication

    Vmax=210 pmol/min/mg enzyme for PAH uptake1 Publication

    GO - Molecular functioni

    1. chloride ion binding Source: Ensembl
    2. inorganic anion exchanger activity Source: UniProtKB
    3. organic anion transmembrane transporter activity Source: UniProtKB
    4. sodium-independent organic anion transmembrane transporter activity Source: Ensembl

    GO - Biological processi

    1. alpha-ketoglutarate transport Source: UniProtKB
    2. anion transport Source: MGI
    3. organic anion transport Source: UniProtKB
    4. protein homooligomerization Source: Ensembl
    5. renal tubular secretion Source: UniProtKB
    6. response to methotrexate Source: Ensembl
    7. sodium-independent organic anion transport Source: Ensembl

    Enzyme and pathway databases

    ReactomeiREACT_198604. Organic anion transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 6
    Alternative name(s):
    Kidney-specific transport protein
    Novel kidney transcript
    Short name:
    mNKT
    Organic anion transporter 1
    Renal organic anion transporter 1
    Short name:
    mROAT1
    Gene namesi
    Name:Slc22a6
    Synonyms:Nkt, Oat1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:892001. Slc22a6.

    Subcellular locationi

    Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication
    Note: Localized to the plasma membrane.

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. caveola Source: Ensembl
    3. integral component of plasma membrane Source: UniProtKB
    4. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi39 – 391N → Q: Complete loss of PAH transport activity. 1 Publication
    Mutagenesisi49 – 491C → A: Decrease in the level of cell surface expression and transport function. Complete loss of transport function; when associated with A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434. 1 Publication
    Mutagenesisi122 – 1221C → A: Decrease in the level of cell surface expression and transport function. Complete loss of transport function; when associated with A-49; A-78; A-99; A-172; A-183; A-200; A-362; A-335; A-379; A-402; A-427 and A-434. 1 Publication
    Mutagenesisi183 – 1831C → A: Decrease in the level of cell surface expression and transport function. Complete loss of transport function; when associated with A-49; A-78; A-99; A-122; A-172; A-200; A-362; A-335; A-379; A-402; A-427 and A-434. 1 Publication
    Mutagenesisi434 – 4341C → A: Decrease in the level of cell surface expression and transport function. 80% decrease in the level of transport activity; when associated with A-49; A-122 and A-183. Complete loss of transport function; when associated with A-49; A-78; A-99; A-122; A-172; A-183; A-200; A-362; A-335; A-379; A-402 and A-427. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 545545Solute carrier family 22 member 6PRO_0000324168Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi56 – 561N-linked (GlcNAc...)1 Publication
    Glycosylationi86 – 861N-linked (GlcNAc...)1 Publication
    Glycosylationi91 – 911N-linked (GlcNAc...)1 Publication
    Glycosylationi107 – 1071N-linked (GlcNAc...)1 Publication
    Glycosylationi178 – 1781N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Glycosylated. Glycosylation is necessary for proper targeting of the transporter to the plasma membrane.1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiQ8VC69.
    PaxDbiQ8VC69.
    PRIDEiQ8VC69.

    Expressioni

    Tissue specificityi

    Expressed in kidney; in the basolateral membrane and at much lower levels in brain.2 Publications

    Developmental stagei

    Developmentally regulated with significant expression beginning at E18 and rising just before birth.

    Gene expression databases

    BgeeiQ8VC69.
    GenevestigatoriQ8VC69.

    Interactioni

    Structurei

    3D structure databases

    ProteinModelPortaliQ8VC69.
    SMRiQ8VC69. Positions 133-503.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 99CytoplasmicSequence Analysis
    Topological domaini31 – 12999ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini151 – 1577CytoplasmicSequence Analysis
    Topological domaini178 – 1803ExtracellularSequence Analysis
    Topological domaini202 – 21817CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini240 – 2423ExtracellularSequence Analysis
    Topological domaini264 – 33168CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini353 – 36210ExtracellularSequence Analysis
    Topological domaini384 – 3896CytoplasmicSequence Analysis
    Topological domaini411 – 4199ExtracellularSequence Analysis
    Topological domaini441 – 45010CytoplasmicSequence Analysis
    Topological domaini472 – 4787ExtracellularSequence Analysis
    Topological domaini500 – 54546CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei10 – 3021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei130 – 15021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei158 – 17720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei181 – 20121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei219 – 23921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei243 – 26321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei332 – 35221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei363 – 38321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei390 – 41021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei420 – 44021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei451 – 47121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei479 – 49921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domaini

    Multiple cysteine residues are necessary for proper targeting to the plasma membrane.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00750000117345.
    HOGENOMiHOG000234569.
    HOVERGENiHBG108433.
    InParanoidiQ8VC69.
    KOiK08203.
    OMAiMIRQTGM.
    OrthoDBiEOG7C8GH9.
    PhylomeDBiQ8VC69.
    TreeFamiTF315847.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8VC69-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFNDLLKQV GGVGRFQLIQ VTMVVAPLLL MASHNTLQNF TAAIPAHHCR    50
    PPANANLSKD GGLEAWLPLD KQGRPESCLR FPFPHNGTEA NGTGVTEPCL 100
    DGWVYDNSTF PSTIVTEWNL VCSHRAFRQL AQSLFMVGVL LGAMMFGYLA 150
    DRLGRRKVLI LNYLQTAVSG TCAAYAPNYT VYCIFRLLSG MSLASIAINC 200
    MTLNMEWMPI HTRAYVGTLI GYVYSLGQFL LAGIAYAVPH WRHLQLAVSV 250
    PFFVAFIYSW FFIESARWYS SSGRLDLTLR ALQRVARING KQEEGAKLSI 300
    EVLQTSLQKE LTLNKGQASA MELLRCPTLR RLFLCLSMLW FATSFAYYGL 350
    VMDLQGFGVS MYLIQVIFGA VDLPAKFVCF LVINSMGRRP AQLASLLLAG 400
    ICILVNGIIP RGHTIIRTSL AVLGKGCLAS SFNCIFLYTG ELYPTMIRQT 450
    GLGMGSTMAR VGSIVSPLIS MTAEFYPSIP LFIFGAVPVA ASAVTALLPE 500
    TLGQPLPDTV QDLKSRSRGK QKQQQLEQQK QMIPLQVSTQ EKNGL 545
    Length:545
    Mass (Da):60,013
    Last modified:March 1, 2002 - v1
    Checksum:i827782E115705C77
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti66 – 661W → R in AAC53112. (PubMed:9045672)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52842 mRNA. Translation: AAC53112.1.
    AK035971 mRNA. Translation: BAC29261.1.
    BC021647 mRNA. Translation: AAH21647.1.
    CCDSiCCDS29538.1.
    RefSeqiNP_032792.2. NM_008766.3.
    UniGeneiMm.30090.

    Genome annotation databases

    EnsembliENSMUST00000010250; ENSMUSP00000010250; ENSMUSG00000024650.
    GeneIDi18399.
    KEGGimmu:18399.
    UCSCiuc008gme.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U52842 mRNA. Translation: AAC53112.1 .
    AK035971 mRNA. Translation: BAC29261.1 .
    BC021647 mRNA. Translation: AAH21647.1 .
    CCDSi CCDS29538.1.
    RefSeqi NP_032792.2. NM_008766.3.
    UniGenei Mm.30090.

    3D structure databases

    ProteinModelPortali Q8VC69.
    SMRi Q8VC69. Positions 133-503.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi Q8VC69.
    ChEMBLi CHEMBL5653.

    Proteomic databases

    MaxQBi Q8VC69.
    PaxDbi Q8VC69.
    PRIDEi Q8VC69.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000010250 ; ENSMUSP00000010250 ; ENSMUSG00000024650 .
    GeneIDi 18399.
    KEGGi mmu:18399.
    UCSCi uc008gme.2. mouse.

    Organism-specific databases

    CTDi 9356.
    MGIi MGI:892001. Slc22a6.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00750000117345.
    HOGENOMi HOG000234569.
    HOVERGENi HBG108433.
    InParanoidi Q8VC69.
    KOi K08203.
    OMAi MIRQTGM.
    OrthoDBi EOG7C8GH9.
    PhylomeDBi Q8VC69.
    TreeFami TF315847.

    Enzyme and pathway databases

    Reactomei REACT_198604. Organic anion transport.

    Miscellaneous databases

    ChiTaRSi SLC22A6. mouse.
    NextBioi 294008.
    PROi Q8VC69.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q8VC69.
    Genevestigatori Q8VC69.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00898. 2A0119. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of NKT, a gene product related to the organic cation transporter family that is almost exclusively expressed in the kidney."
      Lopez-Nieto C.E., You G., Bush K.T., Barros E.J., Beier D.R., Nigam S.K.
      J. Biol. Chem. 272:6471-6478(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, MUTAGENESIS OF CYS-49; CYS-78; CYS-99; CYS-122; CYS-172; CYS-183; CYS-200; CYS-326; CYS-335; CYS-379; CYS-402; CYS-427 AND CYS-434, SUBCELLULAR LOCATION.
      Strain: BALB/c.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    4. "Heterologous expression and functional characterization of a mouse renal organic anion transporter in mammalian cells."
      Kuze K., Graves P., Leahy A., Wilson P., Stuhlmann H., You G.
      J. Biol. Chem. 274:1519-1524(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
    5. "Regulation of mOAT-mediated organic anion transport by okadaic acid and protein kinase C in LLC-PK(1) cells."
      You G., Kuze K., Kohanski R.A., Amsler K., Henderson S.
      J. Biol. Chem. 275:10278-10284(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Cysteine residues in the organic anion transporter mOAT1."
      Tanaka K., Zhou F., Kuze K., You G.
      Biochem. J. 380:283-287(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Role of glycosylation in the organic anion transporter OAT1."
      Tanaka K., Xu W., Zhou F., You G.
      J. Biol. Chem. 279:14961-14966(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASN-39, GLYCOSYLATION AT ASN-56; ASN-86; ASN-91 AND ASN-107.

    Entry informationi

    Entry nameiS22A6_MOUSE
    AccessioniPrimary (citable) accession number: Q8VC69
    Secondary accession number(s): Q61185
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3